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All Journal International Journal of Biosciences and Biotechnology Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Jurnal Ilmu Lingkungan Microbiology Indonesia Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Bioeksperimen: Jurnal Penelitian Biologi SAINS TANAH - Journal of Soil Science and Agroclimatology Adi Widya : Jurnal Pengabdian Masyarakat Jurnal Sinergitas PkM & CSR Jurnal Ecolab Jurnal Litbang Provinsi Jawa Tengah Prosiding Konferensi Nasional PKM-CSR ENVIRO: Journal of Tropical Environmental Research Bioeksperimen
Retno Rosariastuti
Department Of Soil Science, Faculty Of Agriculture, Sebelas Maret University Surakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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Identification and pesticide degradation test of bacterial consortium of contaminated soil Rosariastuti, Retno; Rahmawati, Yulia; Sumani, Sumani; Hartati, Sri
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 1 (2023): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i1.62920

Abstract

Excessive use of pesticides in the process of cultivating shallots results in contamination of the soil. Indigenous bacteria in soil that contaminated with the chlorpyrifos pesticide suspected could used as bioremediation agent of soil contaminated with chlorpyrifos pesticide. The purpose of this study was to identify and obtain a consortium of bacteria capable in degrading pesticides on shallot fields contaminated with the pesticide chlorpyrifos. The method used in this research is the bacteria isolation using soil extract, morphological identification, molecullar identification using Next Generation Sequencing (NGS), analysis of bacterial diversity index and consortium bacterial degradation test in order to reduce levels of the chlorpyrifos pesticide. The results of this study found 16 isolates of bacteria at each study site that were resistant to 100ppm of the pesticide chlorpyrifos so that these isolates were chosen to be used as a consortium of bacteria. Molecular identification of the bacterial consortium showed that there were 10 genera consisting of Cutibacterium, Streptomyces, Staphylococcus, Ensifer, Ochrobactrum, Achromobacter, Escherichia shigella, Klebsiella, Acinetobacter, and Pseudomonas. The bacterial diversity index in shallot soils reached 2,040 and 1,467 on forest soils The reduction efficiency of the bacterial consortium using the method of growing cells and supernatant were 94.48% and 98.88%, respectively.
Viability of Agrobacterium sp. I26 and Agrobacterium sp. I30 in The Carrier Formula of Mocaf Solid Waste, Peat and Manure Rosariastuti, Retno; Nur Sabilla, Widya Putri; Sudadi, Sudadi; Dewi, Widyatmani Sih
ENVIRO: Journal of Tropical Environmental Research Vol 24, No 2 (2022): September
Publisher : Center for Environmental Research, LPPM, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/enviro.v24i2.70354

Abstract

Agrobacterium sp. I26 and Agrobacterium sp. I30 are bioremediation agent that can be used as functional bacteria in biofertilizers. The storage of these bacteria requires carrier. Not all carriers can support bacterial viability, so it was necessary to examine carrier formulas as basic ingredients for biofertilizers which the quality standards based on the Minister of Agriculture Regulation No. 70/2011. This research aimed to: (1) study the viability of Agrobacterium sp. I26 and Agrobacterium sp. I30 in some carrier formulas; and (2) obtain the best carrier formula to support the viability of Agrobacterium sp. I26 and Agrobacterium sp. I30. Research design was factorial using completely randomized design (CRD) as the based design, consisting of 2 factors: 1) Carrier Fornula (C): C1; C2; C3; C4; C5, 2) Bacteria (I): I1; I2; I3, so there were 15 treatment combinations each was repeated 4 times, thus there were 60 experimental units. The results showed that Agrobacterium sp. I26 has better viability than Agrobacterium sp. I30 during 90 days incubation period. The best bacterial viability with total bacterial as the indicator was C4 carrier formula: 74 x 10­13 cfu.g-1 for Agrobacterium sp. I26 and C3 carrier formula: 155 x 1012 cfu.g-1.
PENDAMPINGAN PENGEMBANGAN KEBUN BIBIT PLASMA NUTFAH UNTUK KONSERVASI TANAMAN HUTAN BERNILAI BUDAYA TANAMAN SONOKELING DI KHDTK GUNUNG BROMO Supriyadi; Malihatun Nufus; Muhammad Iqbal Nur Madjid; Manggar Arum Aristri; Purwanto; MMA Retno Rosariastuti; Widya Aryani
Jurnal Sinergitas PKM & CSR Vol. 10 No. 2 (2025): February
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v10i2.10848

Abstract

Pohon sonokeling (Dalbergia latifolia) merupakan pohon penghasil kayu yang berkualitas tinggi. Kayu ini juga menjadi komoditas unggulan ekspor di Indonesia yang tergolong kelas awet I dan kelas kuat II dengan peminat paling tinggi dari negara China. Sonokeling masuk ke dalam Appendix 2 CITES sejak tahun 1997, akan tetapi statusnya saat ini rentan punah (VUA1 cd ver 2.3) menurut IUCN Red list. Oleh karena itu, perlu upaya pelestarian pohon sonokeling untuk dapat mempertahankan keberadaannya dan tetap bisa digunakan serta dimanfaatkan sebagai kayu komoditas unggulan Indonesia. KHDTK Gunung Bromo sebelumnya merupakan kawasan pengembangan sonokeling, namun saat ini jumlahnya menurun. Berdasarkan hal tersebut, perlu pengembangan pohon sonokeling dari berbagai sisi, baik untuk menjaga kelestarian pohon sonokeling itu sendiri berupa penyimpanan plasma nutfah maupun menjadi sumber ekonomi dalam produksi kayu komersial. Melalui kegiatan pengabdian ini, tim pengabdian menggandeng pengelola KHDTK Gunung Bromo dan masyarakat sekitar kawasan, yaitu KTH Putri Serang. Tujuan dari dilakukannya kegiatan pengabdian adalah untuk meningkatkan pemahaman dan keterampilan anggota KTH dalam pengelolaan pohon sonokeling. Hal ini juga untuk mendukung upaya konservasi pohon sonokeling. Pengabdian dilakukan dengan metode pendekatan participatory rapid appraisal atau PRA. Selain itu, juga dilakukan pengembangan persemaian pohon sonokeling untuk memudahkan anggota KTH dalam pengelolaannya. Berdasarkan hasil kegiatan pengabdian, terdapat peningkatan pengetahuan dan keterampilan anggota KTH Putri Serang terkait pengelolaan pohon sonokeling.
Co-Authors ABDI LEONARDO SARAGIH Aji, Alfian Chrisna Alfian Chrisna Aji Ali Pramono Ali Pramono Aryani, Widya Azzahra, Nadine Yuki Candraningtyas, Callista Fabiola Darsowiyono, Supriyadi Dewi Handayani Dwi Priyo Ariyanto Endhah Wijoyo Utomo Hardita Librasanti Sudarmawan Hery Widijanto Irfan Dwidya Prijambada Irfan Dwidya Prijambada Jauhari Syamsiyah Joko Winarno Karisma Ana Yasinta Kharis Triyono Kokyo Oh Mahdani, Sutheta Putra Manggar Arum Aristri Marlia Pangestuti Masyithoh, Galuh Merkuria Karyantina Mohammad Masykuri MUHAMMAD ARDIAN NURSETYAWAN Muhammad Iqbal Nur Madjid N Ngadiman Ngadiman Ngadiman Nugraeni L Nur Sabilla, Widya Putri P Purwanto PRAMUSITA YOGA DANISWARA Pungky Ferina Purwanto Purwanto Purwanto Purwanto Purwanto Rahayu Rahayu Rohmatulaeny, Wahyu Zolla Rony, Zahara Tussoleha S Supriyadi Sachiko Takahi Saputri, Agustin Ayu Sari, Cynthia Permata Selly Maisyarah Sri Budiastuti Sri Hartati Sri Hartati Sri Hartati Sri Wedhastri Sudadi Sudadi Sumani . Sumani Sumani Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Umi Barokah Vita Ratri Cahyani Widya Aryani Widyatmani Sih Dewi Wiwin Widiastuti Wiwin Widiastuti Yulia Rahmawati, Yulia