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Fitoremediasi Logam Kromium di Tanah Sawah dengan Rami (Boehmeria nivea) dan Environmental Health Agriculture System (EHAS) Alfian Chrisna Aji; Mohammad Masykuri; Retno Rosariastuti
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9232

Abstract

Chromium metal is one of the heavy metal wastes from various industries and is persistent for the agricultural environment, especially in rice fields. Chromium metal can change biodiversity and ecosystem function in paddy soil. Chromium metal phytoremediation that pollutes paddy soils with hemp (Boehmeria nivea) is important because paddy soils play a role as a living medium for food crops, especially rice (Oryza sativa). One indicator of the success of phytoremediation is the reduction of chromium metal content in the soil, so it requires a policy system to maintain a healthy environmentally friendly agriculture. This study aimed to determine the ability of Boehmeria nivea to reduce levels of chromium metal in the soil and provide policy solutions to keep environmentally healthy agriculture. This study used a complete randomized block design, random sampling of chromium metal data. The results showed the initial concentration of chromium metal in the soil was 2.36 ppm, after treatment with the interaction between Agrobacterium sp. I3 with Boehmeria nivea (P0B1T1) and interaction of organic matter (compost) with Boehmeria nivea (P0B2T1) obtained Cr 1.37 ppm metal content with a decrease of 42.01%. The resulting policy solution is the Environment Health Agriculture System (EHAS). The conclusion of this study was phytoremediation of chromium metal using Boehmeria nivea combined with the Environment Health Agriculture System can create a healthy environmentally friendly agricultural system.
Remediation of Chromium Contaminated Soil by Phyto-Bio System (PBS) Application Retno Rosariastuti; Selly Maisyarah; Sudadi Sudadi; Sri Hartati; Purwanto Purwanto
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 16, No 1 (2019): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v16i1.24932

Abstract

Chromium polluted water was increased as the result of the growth of the industries, due to their industrial waste were most likely contain heavy metals, especially textile industrial waste that was discarded to the rivers. This research aimed to study the removal of chromium levels in soil used the symbiosis between plant and bacteria. Soil sample contained with 4.33 mg kg-1 and the irrigation water sample contained with 1.09 mg l-1 of total chromium. This research design was factorial with Randomized Complete Block Design as the based design There were 3 factors in this study: 1.  inorganic fertilizer (P): P0: without inorganic fertilizer, P1: with inorganic fertilizer; 2. chelator (B):B0: without chelator, B1: with chelator Rhizobium sp I3, B2: with chelator manure; 3. Plant (T): T0 without plant, T1: with plant. Data were analyzed by statistical analysis using ANOVA continued by T-test or Duncan Multiple Range test and correlation test. The result showed that the remediation process reduced chromium levels in soil with the removal effectivity up to 71.90% on the treatment combination of NPK fertilizer+manure+plant while removal effectivity on plant-only treatment was 55.66%. The chromium levels in Fimbristylis globulosa were in the range from 1.82–3.15 μ g-1, it indicated that Fimbristylis globulosa was a feasible plant for bioremediation. Fimbristylis globulosa grew well and has the ability to absorb chromium, especially by combining it with Rhizobium sp I3and the chromium uptake in roots was higher than shoots.
PERAN RHIZOBAKTERI DALAM FITOEKSTRAKSI LOGAM BERAT KROMIUM PADA TANAMAN JAGUNG MMA Retno Rosariastuti; Ali Pramono; Ngadiman Ngadiman; Irfan D Prijambada
Jurnal Ecolab Vol 6, No 1 (2012): Jurnal Ecolab
Publisher : Pusat Standardisasi Instrumen Kualitas Lingkungan Hidup Laboratorium Lingkungan (P3KLL)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jklh.2012.6.1.38-50

Abstract

Logam berat bersifat persisten dalam lingkungan dan diketahui mengubah biodiversitas, struktur dan fungsi ekosistem tanah. Remediasi tanah terkontaminasi logam menjadi penting, karena tanah sebagai media penghasil bahan pangan. Pendekatan konvensional untuk remediasi tempat yang terkontaminasi logam meliputi fisika dan kimia, namun aplikasi proses-proses ini terbatas karena kendala teknologi dan ekonomi. Oleh karena itu diperlukan metode remediasi yang murah, aman dan ramah lingkungan seperti bioremediasi. Penelitian ini bertujuan untuk mengetahui kemampuan rhizobakteri dalam membantu fitoekstraksi logam berat kromium pada tanaman jagung. Tahapan penelitian meliputi 1) uji toleransi bakteri terhadap Cr(VI), 2) uji reduksi Cr(VI), dan 3) uji serapan Cr(VI) pada tanaman jagung. Hasil penelitian menunjukkan bahwa Isolat 27 toleran terhadap toksisitas Cr(VI) pada konsentrasi 15 ppm. Isolat 27 mereduksi Cr(VI) secara sempurna dalam waktu 18 jam. Isolat 27 membantu fitoekstraksi logam kromium pada tanaman jagung sebesar 241 kali dibandingkan kontrol. Perlu penelitian lebih lanjut untuk mengetahui jenis asam yang berperan dalam mekanisme fitoekstraksi pada tanaman jagung. Isolat 27 dapat digunakan sebagai inokulan dalam fitoremediasi dan pemacu pertumbuhan tanaman.
The effectiveness of Mendong plant (Fimbrystilis globulosa) as a phytoremediator of soil contaminated with chromium of industrial waste Pungky Ferina; Retno Rosariastuti; S Supriyadi
Journal of Degraded and Mining Lands Management Vol 4, No 4 (2017)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.163 KB) | DOI: 10.15243/jdmlm.2017.044.899

Abstract

The textile industry produces sideline output in the form of dangerous waste. The textile industrial waste containing heavy metal, one of which is Chromium (Cr).  Chromium is very dangerous metal for environment, especially chromium hexavalent that has properties of soluble, carcinogenic, and toxic. The pollution of chromium in soil is a problem that the action to be taken with the technology of bioremediation. Phytoremediation of soil contaminated with chromium using Mendong plant (Fimbrystilis globulosa), combined with association of microorganisms Agrobacterium sp I3 and compost. This study was conducted in field experiment plots using a completely randomized block design. Data were analyzed using Anova followed by Duncan and correlation tests. The results showed that the Mendong plant was an effective phytoremediator of soil contaminated with chromium and it can be used as a chromium accumulator plant. The highest decrease of soil chromium content of 58.39% was observed on the combined artificial fertilizer, Agrobacterium sp I3 and Mendong plant treatment (P1B1T1). Removal effectiveness of chromium at the treatments using Mendong plant was higher than without the Mendong plant. Chromium uptake in shoots was higher than in roots of Mendong plant. Bioremediation increased the total bacterial colonies, decreased soil pH, and increased cation exchange capacity of the soil. The growth of the Mendong plant was in a good condition during the process of bioremediation.
The Utilization of Modified Cassava Flour (Mocaf) Industry Waste and Peat as Carrier of Nitrogen-Fixing Bacteria and Phosphate Solubilizing Bacteria Inoculant RETNO ROSARIASTUTI; SUMANI SUMANI; SUPRIYADI SUPRIYADI; MUHAMMAD ARDIAN NURSETYAWAN; PRAMUSITA YOGA DANISWARA
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.608 KB) | DOI: 10.5454/mi.11.4.1

Abstract

Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier for  a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers,  because of its nutrient content. The aim of this study was  determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubation  of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. There  were  three  types of  carrier  which  have different  combination. The base material were solid  waste  of  Mocaf  industry   and  peat.  All materials  of carrier  were  mixed and sterilized, than inoculated by Nitrogen- Fixing  Bacteria (NFB)  and  Phosphate Solubilizing  Bacteria (PSB) and incubated  for  60  days. The  growth of  bacterias were analyzed  every 15  days  and  the  chemical composition  of  carrier  were  analyzed  at  the begin and the  end of  research   (incubation).    The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.
The Use of Agrobacterium sp.I3 and Compost as Chelator Combined by NPK 3 Fertilizer and Mendong Plant (Fimbristylis sp.) in Bioremediation of Paddy Soil Contaminated by Lead (Pb) RETNO ROSARIASTUTI; ABDI LEONARDO SARAGIH; SUDADI SUDADI; SUPRIYADI SUPRIYADI; WIWIN WIDIASTUTI
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1888.536 KB) | DOI: 10.5454/mi.13.3.1

Abstract

Industrial waste supplies contains heavy metals such as Pb which will cause pollution in paddy fields. Remediation of paddy soil contaminated by Pb heavy metal must be done by simple, environmental friendly, cheap and sustainable technology, that is bioremediation. The purpose of this study was to study the effectiveness of bioremediation using Agrobacterium sp. I3 and compost as chelator combined by Mendong plant and NPK fertilizer, and learn the ability of Mendong in uptaking metal soil Pb. This was field experimental research, had a factorial patern, using Completly Randomized Block Design as the base design, with three factors: (1) NPK fertilizers (P0: no NPK fertilizers, P1: with NPK fertilizers), (2) Chelator (K0: no chelator; K1: with chelator Agrobacterium sp. I3 ; K2: with chelator compost); and (3) Plant (T0: without plant; T1: with Mendong plant). The results showed that Agrobacterium sp. I3 and compost were increasing Pb uptake in shoot, but decreasing Pb uptake in root. Mendong plant has highly ability in uptaking soil Pb, so decreased soil Pb, and effective as the phytoremediator. NPK fertilizer increased plant growth so increased Pb uptaken by plant. The highest Pb uptake was in treatment combination of NPK fertilizer + Mendong plant: 80.916 µg, followed by NPK fertilizer + Agrobacterium sp. I3 + Mendong Plant: 76.363 µg. The highest decreased of soil Pb (42.41%) was found in treatment combination of compost + Mendong Plant. Key words: Agrobacterium sp.I3, compost, Fimbristylis sp, Pb, phytoremediation
Pendampingan Integrasi Kacang Sacha Inchi untuk Mendukung Pengelolaan Hutan Lestari di KTH Sumber Wono, Banyurip, Jenar, Sragen DARSOWIYONO, SUPRIYADI; Purwanto, Purwanto; Rosariastuti, MMA Retno; Hartati, Sri; Masyithoh, Galuh; Nufus, Malihatun; Aryani, Widya
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 6 (2023): INOVASI PERGURUAN TINGGI & PERAN DUNIA INDUSTRI DALAM PENGUATAN EKOSISTEM DIGITAL & EK
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v6i0.1863

Abstract

Desa Banyurip merupakan salah satu desa yang termasuk dalam zona merah penduduk miskin. Desa Banyurip berada di kawasan sekitar hutan jati yang dikelola oleh BKPH Tangen, KPH Surakarta. Hal ini menyebabkan banyak masyarakat yang mengandalkan penghasilkan terhadap hasil hutan. Seiring dengan meningkatnya kesadaran masyarakat akan pentingnya jasa lingkungan yang dibentuk oleh hutan, maka masyrakat menerapkan pengelolaan hutan lestari melalui agroforestri. Pada pola agroforestri perlu dilakukan integrasi tanaman bawah tegakan yang memiliki nilai ekonomi tinggi, sehingga hutan dapat menghasilkan kayu keras, tanaman pertanian, sekaligus pakan ternak. LMDH dan KTH Sumber Wono Desa Banyurip telah merintis introduksi tanaman Sacha Inchi (Plukenetia volubilis) di bawah tegakan jati Perum Perhutani dengan sistem kemitraan. Biji yang dihasilkan merupakan superfood yang kaya vitamin, omega 3, omega 6 dan omega 9, sehingga memiliki harga yang tinggi. Tujuan dan manfaat budidaya Sacha Inchi yaitu memperoleh manfaat ekonomi yang tinggi dan hutan menjadi lestari. Oleh karena itu, pendampingan integrasi kacang sacha inchi dapat menjadi salah satu strategi dalam penyelamatan hutan dan meningkatkan perekonomian masyarakat desa. Berdasarkan diskusi dengan LMDH dan KTH Sumber Wono, diperlukan pendampingan budidaya sacha inchi dan kelembagaan pada aspek-aspek on farm dan off farm.Kabupaten Sragen memiliki jumlah penduduk miskin sebanyak 12,792% dari jumlah total penduduknya. Desa Banyurip merupakan salah satu desa yang termasuk dalam zona merah penduduk miskin. Desa Banyurip berada di kawasan sekitar hutan jati yang dikelola oleh BKPH Tangen, KPH Surakarta. Hal ini menyebabkan banyak masyarakat yang mengandalkan penghasilkan terhadap hasil hutan. Seiring dengan meningkatnya kesadaran masyarakat akan pentingnya jasa lingkungan yang dibentuk oleh hutan, maka masyrakat dapat menerapkan pengelolaan hutan lestari melalui agroforestri. Agroforestri adalah istilah untuk sistem dan teknik pengelolaan lahan yang menggabungkan tanaman keras berkayu pada lahan yang sama dengan tanaman dan hewan pertanian. Pada pola agroforestri perlu dilakukan integrasi tanaman bawah tegakan yang memiliki nilai ekonomi tinggi, sehingga hutan dapat menghasilkan kayu keras, tanaman pertanian, sekaligus pakan ternak. LMDH dan KTH Sumber Wono Desa Banyurip telah merintis introduksi tanaman Sacha Inchi (Plukenetia volubilis) di bawah tegakan jati Perum Perhutani dengan sistem kemitraan. Biji yang dihasilkan merupakan superfood yang kaya vitamin, omega 3, omega 6 dan omega 9, sehingga memiliki harga yang tinggi. Tujuan dan manfaat budidaya Sacha Inchi yaitu memperoleh manfaat ekonomi yang tinggi dan hutan menjadi lestari. Oleh karena itu, pendampingan integrasi kacang sacha inchi dapat menjadi salah satu strategi dalam penyelamatan hutan dan meningkatkan perekonomian masyarakat desa.
Bioremediation of chlorpyrifos-contaminated soil with biduri plant (Calotropis gigantea), bacteria, and biochar: Soil fertility impact Rosariastuti, Retno; Mahdani, Sutheta Putra; Purwanto, Purwanto; Hartati, Sri; Sumani, Sumani
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5509

Abstract

During the Industrial Revolution, agriculture was linked to environmental issues. One issue is pesticide pollution. Bioremediation is needed because plant diseases and pests cause farmers to use pesticides excessively, polluting and decreasing soil production. This study evaluated soil fertility from bioremediation with biduri plants, bacteria, and biochar on chlorpyrifos-polluted shallot-growing soil in Brebes Regency. This type of research was an experiment in a greenhouse, using a factorial experimental design with a completely randomized design, consisting of two factors, namely bioremediation agent (A0: without bioremediation agent, A1: Atlantibacter hermannii, A2: bacterial consortium) and coconut shell biochar (B0: without biochar, B1: with biochar). There were six treatment combinations, then replicated three times for each treatment, so there were 18 treatment units. The data were statistically analyzed using ANOVA, Duncan Multiple Range Test, and Pearson correlation. The results showed that the best treatments that were able to improve soil fertility after bioremediation were treatments A1B0 (Atlantibacter hermannii + without biochar), A2B0 (bacterial consortium + without biochar), and A2B1 (bacterial consortium + with biochar). The A1B0 treatment (Atlantibacter hermannii + without biochar) increased the pH by 4.97%, and the soil available P was 12 times higher than the initial soil. The A2B0 (bacterial consortium + without biochar) treatment increased soil cation exchange capacity by 99.81% and soil organic C by 116% higher than the initial soil. The A2B1 (bacterial consortium + with biochar) treatment increased soil total N by 37.50% and available K by 38.47%, higher than the initial soil.
Evaluation of Arbuscular Mycorrhizal Cultures in Increasing Phosphorus Uptake and Maize Growth Compared to Chemical and Organic Fertilizers on an Andisol Cahyani, Vita Ratri; Azzahra, Nadine Yuki; Rosariastuti, Retno
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.3867

Abstract

There is still limited information about the formulation of arbuscular mycorrhizal (AM) fungi culture with the specific ability to overcome P retention in Andisols. The purpose of this study is to evaluate the functional ability of eight AM fungi cultures consisting of four cultures from the generation I (A1I31, A2I21, A0I31, and A0I21) and four cultures from generation II (A1I32, A2I22, A0I32, and A0I22) in dealing with P constraints on an Andisol, compared with the application of fresh AM fungi inocula isolated from natural soils, synthetic chemical fertilizers (CF), rice straw (RS) compost, and several combination treatments including Bio-RP Nutrition. The highest functional ability in increasing P uptake and maize growth on Andisol is obtained by A1I32, followed by A2I22 and A0I22, indicating that AM fungi cultures generation II exhibited higher effectiveness than generation I. The increase of P uptake and maize shoot dry weight yielded by those three AM fungi cultures were in the range of 80-97% and 89-103% of T14 (CF 100%), indicating the high potential biofertilizers for reducing the use of chemical fertilizers. By  cultivation  plate  method,  the  present  findings  also  confirmed that  AM   fungi   inoculation   affecting  significantly   the   abundance and  the  composition  of  foliar  endophytic  bacterial  communities.
IMPLEMENTASI PERTANIAN RAMAH LINGKUNGAN DALAM PENGELOLAAN DAS BERBASIS EKONOMI SIRKULER Purwanto, Purwanto; Supriyadi, Supriyadi; Nufus, Malihatun; Sari, Cynthia Permata; Rosariastuti, MMA Retno; Hartati, Sri
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 7 (2024): PKMCSR2024: Kolaborasi Hexahelix dalam Optimalisasi Potensi Pariwisata di Indonesia: A
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v7i0.2373

Abstract

Ekonomi sirkular di Indonesia tercakup di dalam Rencana Pembangunan Jangka Menengah Nasional (RPJMN) 2020 – 2024, di bawah Agenda Prioritas Nasional 1, yaitu memperkuat ketahanan ekonomi untuk pertumbuhan yang berkualitas dan berkeadilan, dan agenda Prioritas Nasional 6: membangun lingkungan hidup, meningkatkan ketahanan bencana, dan perubahan iklim. Pada Prioritas Nasional 6, Ekonomi Sirkular berada dibawah payung Pembangunan Rendah Karbon (PRK) yang juga merupakan salah satu upaya untuk mencapai ekonomi hijau dengan menekankan kegiatannya yakni pengelolaan limbah, pembangunan energi berkelanjutan, dan pengembangan industri hijau. Salah satu komponen PRK adalah Pertanian ramah lingkungan, tidak sekedar untuk meningkatkan produksi tanaman, namun juga bertujuan agar lingkungan tetap sehat, baik pada skala lokal, regional, maupun global. Tidak sekedar untuk mempertahankan produksi pangan, pakan, serat, kayu bakar, mengendalikan erosi, hama, penyakit dan gulma, namun juga bertujuan untuk mengurangi emisi gas rumah kaca, mempertahankan siklus hidrologi serta keanekaragaman hayati. Daerah Aliran Sungai (DAS) berperan penting sebagai daerah tangkapan air yang memiliki peran penting menyediakan kebutuhan air bagi manusia. Bahaya erosi, longsor, hilangnya sumber daya air, dan kesuburan tanah merupakan bahaya dari tidak dikelolanya DAS. Konservasi tanah pada daerah pegunungan di hulu DAS mutlak dilakukan untuk menjamin keberlanjutan produktivitas lahan. Salah satu upaya pengelolaan DAS adalah dengan praktik pertanian ramah lingkungan dengan sistem agroforestri. Desa Sumberejo, Kecamatan Batuwarno, Kabupaten Wonogiri merupakan daerah yang terletak di bagian atas sub DAS Bengawan Solo Hulu. Upaya yang dilakukan adalah penamanam tanaman MPTS (multi purpose tree species) yang sesuai untuk kegiatan rehabilitasi pada lahan hutan rakyat. Selain bermanfaat secara ekologi, penanaman tanaman MPTS juga akan memberikan manfaat secara ekonomi. Tujuan dari kegiatan pengabdian masyarakat ini adalah pendampingan kegiatan rehabilitasi dengan pola agroforestri menggunakan jenis-jenis tanaman prospektif secara ekologi maupun ekonomi dalam mendukung kelestarian. Metode pendekatan yang digunakan dalam studi ini adalah metode participatory rapid appraisal (PRA). Melalui pendekatan ini, masyarakat berpartisipasi dalam pembangunan desa mulai dari perencanaan, pelaksanaan, hingga evaluasi program. Dalam bentuk Participatory Planning yang disertai penganggaran, implementasi, serta monitoring. Hasil dari kegiatan pengabdian ini adalah peningkatan pemahaman terkait perencanaan, penganggaran, serta implementasi dari masyarakat terkait konservasi daerah hulu DAS dengan penanaman tanaman MPTS.