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The Use of Agrobacterium sp.I3 and Compost as Chelator Combined by NPK 3 Fertilizer and Mendong Plant (Fimbristylis sp.) in Bioremediation of Paddy Soil Contaminated by Lead (Pb) RETNO ROSARIASTUTI; ABDI LEONARDO SARAGIH; SUDADI SUDADI; SUPRIYADI SUPRIYADI; WIWIN WIDIASTUTI
Microbiology Indonesia Vol. 13 No. 3 (2019): September 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1888.536 KB) | DOI: 10.5454/mi.13.3.1

Abstract

Industrial waste supplies contains heavy metals such as Pb which will cause pollution in paddy fields. Remediation of paddy soil contaminated by Pb heavy metal must be done by simple, environmental friendly, cheap and sustainable technology, that is bioremediation. The purpose of this study was to study the effectiveness of bioremediation using Agrobacterium sp. I3 and compost as chelator combined by Mendong plant and NPK fertilizer, and learn the ability of Mendong in uptaking metal soil Pb. This was field experimental research, had a factorial patern, using Completly Randomized Block Design as the base design, with three factors: (1) NPK fertilizers (P0: no NPK fertilizers, P1: with NPK fertilizers), (2) Chelator (K0: no chelator; K1: with chelator Agrobacterium sp. I3 ; K2: with chelator compost); and (3) Plant (T0: without plant; T1: with Mendong plant). The results showed that Agrobacterium sp. I3 and compost were increasing Pb uptake in shoot, but decreasing Pb uptake in root. Mendong plant has highly ability in uptaking soil Pb, so decreased soil Pb, and effective as the phytoremediator. NPK fertilizer increased plant growth so increased Pb uptaken by plant. The highest Pb uptake was in treatment combination of NPK fertilizer + Mendong plant: 80.916 µg, followed by NPK fertilizer + Agrobacterium sp. I3 + Mendong Plant: 76.363 µg. The highest decreased of soil Pb (42.41%) was found in treatment combination of compost + Mendong Plant. Key words: Agrobacterium sp.I3, compost, Fimbristylis sp, Pb, phytoremediation
Pendampingan Integrasi Kacang Sacha Inchi untuk Mendukung Pengelolaan Hutan Lestari di KTH Sumber Wono, Banyurip, Jenar, Sragen DARSOWIYONO, SUPRIYADI; Purwanto, Purwanto; Rosariastuti, MMA Retno; Hartati, Sri; Masyithoh, Galuh; Nufus, Malihatun; Aryani, Widya
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 6 (2023): INOVASI PERGURUAN TINGGI & PERAN DUNIA INDUSTRI DALAM PENGUATAN EKOSISTEM DIGITAL & EK
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v6i0.1863

Abstract

Desa Banyurip merupakan salah satu desa yang termasuk dalam zona merah penduduk miskin. Desa Banyurip berada di kawasan sekitar hutan jati yang dikelola oleh BKPH Tangen, KPH Surakarta. Hal ini menyebabkan banyak masyarakat yang mengandalkan penghasilkan terhadap hasil hutan. Seiring dengan meningkatnya kesadaran masyarakat akan pentingnya jasa lingkungan yang dibentuk oleh hutan, maka masyrakat menerapkan pengelolaan hutan lestari melalui agroforestri. Pada pola agroforestri perlu dilakukan integrasi tanaman bawah tegakan yang memiliki nilai ekonomi tinggi, sehingga hutan dapat menghasilkan kayu keras, tanaman pertanian, sekaligus pakan ternak. LMDH dan KTH Sumber Wono Desa Banyurip telah merintis introduksi tanaman Sacha Inchi (Plukenetia volubilis) di bawah tegakan jati Perum Perhutani dengan sistem kemitraan. Biji yang dihasilkan merupakan superfood yang kaya vitamin, omega 3, omega 6 dan omega 9, sehingga memiliki harga yang tinggi. Tujuan dan manfaat budidaya Sacha Inchi yaitu memperoleh manfaat ekonomi yang tinggi dan hutan menjadi lestari. Oleh karena itu, pendampingan integrasi kacang sacha inchi dapat menjadi salah satu strategi dalam penyelamatan hutan dan meningkatkan perekonomian masyarakat desa. Berdasarkan diskusi dengan LMDH dan KTH Sumber Wono, diperlukan pendampingan budidaya sacha inchi dan kelembagaan pada aspek-aspek on farm dan off farm.Kabupaten Sragen memiliki jumlah penduduk miskin sebanyak 12,792% dari jumlah total penduduknya. Desa Banyurip merupakan salah satu desa yang termasuk dalam zona merah penduduk miskin. Desa Banyurip berada di kawasan sekitar hutan jati yang dikelola oleh BKPH Tangen, KPH Surakarta. Hal ini menyebabkan banyak masyarakat yang mengandalkan penghasilkan terhadap hasil hutan. Seiring dengan meningkatnya kesadaran masyarakat akan pentingnya jasa lingkungan yang dibentuk oleh hutan, maka masyrakat dapat menerapkan pengelolaan hutan lestari melalui agroforestri. Agroforestri adalah istilah untuk sistem dan teknik pengelolaan lahan yang menggabungkan tanaman keras berkayu pada lahan yang sama dengan tanaman dan hewan pertanian. Pada pola agroforestri perlu dilakukan integrasi tanaman bawah tegakan yang memiliki nilai ekonomi tinggi, sehingga hutan dapat menghasilkan kayu keras, tanaman pertanian, sekaligus pakan ternak. LMDH dan KTH Sumber Wono Desa Banyurip telah merintis introduksi tanaman Sacha Inchi (Plukenetia volubilis) di bawah tegakan jati Perum Perhutani dengan sistem kemitraan. Biji yang dihasilkan merupakan superfood yang kaya vitamin, omega 3, omega 6 dan omega 9, sehingga memiliki harga yang tinggi. Tujuan dan manfaat budidaya Sacha Inchi yaitu memperoleh manfaat ekonomi yang tinggi dan hutan menjadi lestari. Oleh karena itu, pendampingan integrasi kacang sacha inchi dapat menjadi salah satu strategi dalam penyelamatan hutan dan meningkatkan perekonomian masyarakat desa.
Bioremediation of chlorpyrifos-contaminated soil with biduri plant (Calotropis gigantea), bacteria, and biochar: Soil fertility impact Rosariastuti, Retno; Mahdani, Sutheta Putra; Purwanto, Purwanto; Hartati, Sri; Sumani, Sumani
Journal of Degraded and Mining Lands Management Vol. 11 No. 2 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.112.5509

Abstract

During the Industrial Revolution, agriculture was linked to environmental issues. One issue is pesticide pollution. Bioremediation is needed because plant diseases and pests cause farmers to use pesticides excessively, polluting and decreasing soil production. This study evaluated soil fertility from bioremediation with biduri plants, bacteria, and biochar on chlorpyrifos-polluted shallot-growing soil in Brebes Regency. This type of research was an experiment in a greenhouse, using a factorial experimental design with a completely randomized design, consisting of two factors, namely bioremediation agent (A0: without bioremediation agent, A1: Atlantibacter hermannii, A2: bacterial consortium) and coconut shell biochar (B0: without biochar, B1: with biochar). There were six treatment combinations, then replicated three times for each treatment, so there were 18 treatment units. The data were statistically analyzed using ANOVA, Duncan Multiple Range Test, and Pearson correlation. The results showed that the best treatments that were able to improve soil fertility after bioremediation were treatments A1B0 (Atlantibacter hermannii + without biochar), A2B0 (bacterial consortium + without biochar), and A2B1 (bacterial consortium + with biochar). The A1B0 treatment (Atlantibacter hermannii + without biochar) increased the pH by 4.97%, and the soil available P was 12 times higher than the initial soil. The A2B0 (bacterial consortium + without biochar) treatment increased soil cation exchange capacity by 99.81% and soil organic C by 116% higher than the initial soil. The A2B1 (bacterial consortium + with biochar) treatment increased soil total N by 37.50% and available K by 38.47%, higher than the initial soil.
Evaluation of Arbuscular Mycorrhizal Cultures in Increasing Phosphorus Uptake and Maize Growth Compared to Chemical and Organic Fertilizers on an Andisol Cahyani, Vita Ratri; Azzahra, Nadine Yuki; Rosariastuti, Retno
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.3867

Abstract

There is still limited information about the formulation of arbuscular mycorrhizal (AM) fungi culture with the specific ability to overcome P retention in Andisols. The purpose of this study is to evaluate the functional ability of eight AM fungi cultures consisting of four cultures from the generation I (A1I31, A2I21, A0I31, and A0I21) and four cultures from generation II (A1I32, A2I22, A0I32, and A0I22) in dealing with P constraints on an Andisol, compared with the application of fresh AM fungi inocula isolated from natural soils, synthetic chemical fertilizers (CF), rice straw (RS) compost, and several combination treatments including Bio-RP Nutrition. The highest functional ability in increasing P uptake and maize growth on Andisol is obtained by A1I32, followed by A2I22 and A0I22, indicating that AM fungi cultures generation II exhibited higher effectiveness than generation I. The increase of P uptake and maize shoot dry weight yielded by those three AM fungi cultures were in the range of 80-97% and 89-103% of T14 (CF 100%), indicating the high potential biofertilizers for reducing the use of chemical fertilizers. By  cultivation  plate  method,  the  present  findings  also  confirmed that  AM   fungi   inoculation   affecting  significantly   the   abundance and  the  composition  of  foliar  endophytic  bacterial  communities.
IMPLEMENTASI PERTANIAN RAMAH LINGKUNGAN DALAM PENGELOLAAN DAS BERBASIS EKONOMI SIRKULER Purwanto, Purwanto; Supriyadi, Supriyadi; Nufus, Malihatun; Sari, Cynthia Permata; Rosariastuti, MMA Retno; Hartati, Sri
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 7 (2024): PKMCSR2024: Kolaborasi Hexahelix dalam Optimalisasi Potensi Pariwisata di Indonesia: A
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v7i0.2373

Abstract

Ekonomi sirkular di Indonesia tercakup di dalam Rencana Pembangunan Jangka Menengah Nasional (RPJMN) 2020 – 2024, di bawah Agenda Prioritas Nasional 1, yaitu memperkuat ketahanan ekonomi untuk pertumbuhan yang berkualitas dan berkeadilan, dan agenda Prioritas Nasional 6: membangun lingkungan hidup, meningkatkan ketahanan bencana, dan perubahan iklim. Pada Prioritas Nasional 6, Ekonomi Sirkular berada dibawah payung Pembangunan Rendah Karbon (PRK) yang juga merupakan salah satu upaya untuk mencapai ekonomi hijau dengan menekankan kegiatannya yakni pengelolaan limbah, pembangunan energi berkelanjutan, dan pengembangan industri hijau. Salah satu komponen PRK adalah Pertanian ramah lingkungan, tidak sekedar untuk meningkatkan produksi tanaman, namun juga bertujuan agar lingkungan tetap sehat, baik pada skala lokal, regional, maupun global. Tidak sekedar untuk mempertahankan produksi pangan, pakan, serat, kayu bakar, mengendalikan erosi, hama, penyakit dan gulma, namun juga bertujuan untuk mengurangi emisi gas rumah kaca, mempertahankan siklus hidrologi serta keanekaragaman hayati. Daerah Aliran Sungai (DAS) berperan penting sebagai daerah tangkapan air yang memiliki peran penting menyediakan kebutuhan air bagi manusia. Bahaya erosi, longsor, hilangnya sumber daya air, dan kesuburan tanah merupakan bahaya dari tidak dikelolanya DAS. Konservasi tanah pada daerah pegunungan di hulu DAS mutlak dilakukan untuk menjamin keberlanjutan produktivitas lahan. Salah satu upaya pengelolaan DAS adalah dengan praktik pertanian ramah lingkungan dengan sistem agroforestri. Desa Sumberejo, Kecamatan Batuwarno, Kabupaten Wonogiri merupakan daerah yang terletak di bagian atas sub DAS Bengawan Solo Hulu. Upaya yang dilakukan adalah penamanam tanaman MPTS (multi purpose tree species) yang sesuai untuk kegiatan rehabilitasi pada lahan hutan rakyat. Selain bermanfaat secara ekologi, penanaman tanaman MPTS juga akan memberikan manfaat secara ekonomi. Tujuan dari kegiatan pengabdian masyarakat ini adalah pendampingan kegiatan rehabilitasi dengan pola agroforestri menggunakan jenis-jenis tanaman prospektif secara ekologi maupun ekonomi dalam mendukung kelestarian. Metode pendekatan yang digunakan dalam studi ini adalah metode participatory rapid appraisal (PRA). Melalui pendekatan ini, masyarakat berpartisipasi dalam pembangunan desa mulai dari perencanaan, pelaksanaan, hingga evaluasi program. Dalam bentuk Participatory Planning yang disertai penganggaran, implementasi, serta monitoring. Hasil dari kegiatan pengabdian ini adalah peningkatan pemahaman terkait perencanaan, penganggaran, serta implementasi dari masyarakat terkait konservasi daerah hulu DAS dengan penanaman tanaman MPTS.
Improving Village Community Skills with Local Corn Food Creations in Giriwoyo, Wonogiri Regency: MENINGKATKAN KETERAMPILAN MASYARAKAT DESA DENGAN KREASI BAHAN PANGAN LOKAL JAGUNG DI GIRIWOYO KABUPATEN WONOGIRI Sumarmi, Sumarmi; Triyono, Kharis; Karyantina, Merkuria; Handayani, Dewi; Rosariastuti, MMA Retno
Adi Widya : Jurnal Pengabdian Masyarakat Vol 9 No 1 (2025): Adi Widya: Jurnal Pengabdian Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v9i1.11661

Abstract

This community servis aims to improve the skills of the people of Giriwoyo village, Wonogiri, through training in the creation of local corn food. The methods used include practical training and demonstrations on making various processed corn-based products, such as cakes and snacks. Apart from that, this activity also succeeded in creating awareness of the potential of corn as a food source with economic value. It is hoped that participants can utilize the skills acquired to develop small businesses, increase income, and contribute to the welfare of local communities.
Effect of biological agents on chlorpyrifos content in soil and bulbs, soil fertility, and shallot (Allium ascalonicum L.) yield Saputri, Agustin Ayu; Rahayu, Rahayu; Widijanto, Hery; Cahyani, Vita Ratri; Rosariastuti, Retno
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8735

Abstract

Continuous pesticide application in shallot (Allium ascalonicum L.) cultivation can reduce soil chemical and biological fertility, potentially leading to the accumulation of pesticide residues. Sustainable agricultural practices are therefore needed to restore soil fertility and improve shallot yield. This study was conducted as a field experiment using a randomized complete block design with a single-factor treatment, namely the type of biological agent: control (BA0), Atlantibacter hermannii (BA1), Pseudomonas sp. (BA2), Indigenous bacterial consortium (BA3), Eco enzyme (BA4), and Biofilm (BA5) from Mount Lawu's western slopes. Each experimental treatment was repeated five times, resulting in a total of 30 units. Statistical analysis was done using analysis of variance (ANOVA), followed by Duncan multiple range test (DMRT) and Pearson correlation. The parameters observed included chlorpyrifos levels, chemical and biological characteristics, and shallot yield. The results of the study indicate that Atlantibacter hermannii (BA1) reduced soil chlorpyrifos content by 64.62% and increased shallot yield by 41.44%. Eco enzyme (BA4) and biofilm (BA5) were the most effective in lowering chlorpyrifos accumulation in bulbs, with reductions of 42.31% and 38.46%, respectively. Regarding soil fertility, BA1 increased available phosphorus by 30.32%, Pseudomonas sp. (BA2) increased available potassium by 12%, and BA4 increased total nitrogen by 18.18%; however, the improvements from BA2 and BA4 were similar to those from BA1. Overall, Atlantibacter hermannii (BA1) and eco enzyme (BA4) emerged as the most promising biological agents for reducing pesticide residues, enhancing soil fertility, and increasing shallot production, highlighting their potential application in sustainable agriculture.
Fitoremediasi Logam Kromium di Tanah Sawah dengan Rami (Boehmeria nivea) dan Environmental Health Agriculture System (EHAS) Aji, Alfian Chrisna; Masykuri, Mohammad; Rosariastuti, Retno
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9232

Abstract

Chromium metal is one of the heavy metal wastes from various industries and is persistent for the agricultural environment, especially in rice fields. Chromium metal can change biodiversity and ecosystem function in paddy soil. Chromium metal phytoremediation that pollutes paddy soils with hemp (Boehmeria nivea) is important because paddy soils play a role as a living medium for food crops, especially rice (Oryza sativa). One indicator of the success of phytoremediation is the reduction of chromium metal content in the soil, so it requires a policy system to maintain a healthy environmentally friendly agriculture. This study aimed to determine the ability of Boehmeria nivea to reduce levels of chromium metal in the soil and provide policy solutions to keep environmentally healthy agriculture. This study used a complete randomized block design, random sampling of chromium metal data. The results showed the initial concentration of chromium metal in the soil was 2.36 ppm, after treatment with the interaction between Agrobacterium sp. I3 with Boehmeria nivea (P0B1T1) and interaction of organic matter (compost) with Boehmeria nivea (P0B2T1) obtained Cr 1.37 ppm metal content with a decrease of 42.01%. The resulting policy solution is the Environment Health Agriculture System (EHAS). The conclusion of this study was phytoremediation of chromium metal using Boehmeria nivea combined with the Environment Health Agriculture System can create a healthy environmentally friendly agricultural system.
Recovery of soil carbon pools and C–N stoichiometry under drought in degraded tin-mined soils using organic, inorganic, and bio-amendments RAHAYU, RAHAYU; ERDASWIN, FARHAN; ROSARIASTUTI, RETNO; DEWI, WIDYATMANI SIH; FATIMAH, FATIMAH; HERAWATI, AKTAVIA; ICHSAN, NURUL
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090248

Abstract

Abstract. Rahayu, Erdaswin F, Rosariastuti R, Dewi WS, Fatimah, Herawati A, Ichsan N. 2025. Recovery of soil carbon pools and C–N stoichiometry under drought in degraded tin-mined soils using organic, inorganic, and bio-amendments. Asian J Agric 9: 818-830. Large-scale tin mining on Bangka Island, Indonesia, has severely degraded soils, resulting in low carbon reserves and imbalanced C–N stoichiometry, particularly under seasonal drought. This study evaluated the effectiveness of an integrated amendment strategy to restore soil carbon pools and improve C–N stoichiometry under these challenging conditions. A 100-day screenhouse experiment was conducted on degraded tin-mined soil using a Completely Randomized Design. Treatments included municipal compost, dolomite, Lactic Acid Bacteria (LAB), and NPK fertilizer, applied individually and in combination. Key physicochemical properties, carbon pools, stoichiometric ratios, and their interrelationships were analyzed using ANOVA, Redundancy Analysis, and Pearson correlation. The integrated combination treatment was synergistically superior (ANOVA, p<0.01). The recovery of carbon pools was marked by a seven-fold surge in microbial biomass carbon to 703.73 mg kg-¹ and a near-doubling of the soil organic carbon stock to 29.21 Mg C ha-¹. The improvement in C–N stoichiometry was evidenced by the optimization of key microbial efficiency ratios, with the MBC/SOC ratio reaching 13.99% and the MBC/TN ratio increasing to 45.18%. Redundancy Analysis confirmed that compost-based treatments formed a distinct, functionally efficient group. Furthermore, Pearson correlation revealed the integrated nature of this recovery, showing a tight coupling between the accumulation of key carbon pools (SOC and MBC; r=0.909) and the enhancement of microbial C-use efficiency. In contrast, the dolomite amendment created a dysfunctional system by causing a massive accumulation of Dissolved Organic Carbon (DOC) to 279.18 mg kg-¹ without a corresponding increase in microbial biomass. A holistic, multi-ameliorant strategy anchored by a substantial organic matter base is essential for restoring ecosystem functions. This approach provides a robust and practical framework for the sustainable land management of degraded post-tin mining landscapes, offering a viable pathway to rebuild soil health and enhance drought resilience.
Sumberejo Community's Perspective on Non-Timber Forest Products to Support Sustainable Environment Sari, Cynthia Permata; Supriyadi; Purwanto; Rosariastuti, Retno; Nufus, Malihatun
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.13643

Abstract

This study investigates how Non-Timber Forest Resources (NTFPs) contribute to community livelihoods and environmental sustainability. Through a combination of in-depth interviews and participatory observation, this study highlights the traditional knowledge and practices of the Sumberejo community in managing these resources. This research uses a qualitative approach that combines ethnobotanical surveys, in-depth interviews, and participatory observation with 50 participants, as well as data analysis using Participatory Rapid Appraisal (PRA). The results show that the majority of respondents perceive the potential of NFTPs in the area as very important (56.25% of village officials) and important (45.83% of villagers). Respondents also consider the natural geophysical potential for NFTPs in the area to be very important (68.75% of village officials) and important (50% of villagers). Furthermore, respondents also considered the natural biophysical potential for the NFTPs area to be very important (56.25% of village officials) and important (50% of villagers). The study underscores the potential for integrating NTFP management into broader conservation strategies to promote environmental stewardship and sustainable development. The community's commitment to balancing economic needs with environmental health offers valuable insights for policymakers and conservationists aiming to implement community-based forest management models. This research contributes to a growing body of knowledge on the importance of NTFPs in sustainable environmental governance, showcasing the Sumberejo community's role as stewards of their local ecosystems while maintaining cultural heritage and securing economic well being.