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Journal : Bioscientist : Jurnal Ilmiah Biologi

Isolasi dan Identifikasi Bakteri Asam Laktat pada Pekasam Ikan Haruan: Efek Fermentasi dan Penggorengan Safitri, Diah; Fitriah, Fitriah; Nashihah, Siti; Andika, Andika; Fajeriyati, Nurul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15470

Abstract

This study aims to obtain lactic acid bacteria (LAB) isolates in fermented snakehead fish (Channa striata) pekasam and compare pekasam before and after frying with variations in fermentation time for 1 and 2 weeks. The type of research conducted in this study is experimental, whose testing starts from making pekasam using 30% salt and 50% rice from the total weight of the fish, isolation of LAB, gram staining test, scanning electron microscopy (SEM), and total plate number (ALT) testing. Data were analyzed using SPSS 27 to see significant differences between fermentation and frying times. The results showed that (1) there was LAB growth in all pekasam isolates before and after frying; (2) the results of gram staining showed that all isolates were gram-positive bacteria; (3) the results of SEM observation with a magnification of 12. 000, the morphology of bacillus-shaped bacteria with cell sizes ranging from 1.462 -1.682 µm was obtained; (4) ALT test results of LAB isolates in pekasam before frying in the first week showed results with a value of 1.90 x 106 CFU/mL and the second week 2.04 x 106 CFU/mL while LAB isolates of pekasam after frying in the first week were 0.63 x 106 CFU/mL and the second week 0.75 x 106 CFU/mL; (5) the results of data analysis obtained significant differences between the effects of fermentation and frying on ALT testing, It can be concluded that the longer the fermentation time can increase the total LAB in fermented snakehead fish (pekasam).
Co-Authors Aan Rosiatun Abdul Hoyyi Agus Rusgiyono Agustifa Zea Tazliqoh Al Qarani, Muhammad Aqajahs Altarans, Indra Amin, Ahmad Syaifuddin Amir Fauzi Andika Andika Anik Nurul Aini Apriyanto, Dadan Ramadhan Arsyil Hendra Saputra Aulia, Riskiya Azdina Nur, Faradillah Aziza, Siti Bimbi Ardhana Rizky Brajawikalpa , Rama Samara Damayanti, Almiffa Dani, Sekar Arum Dayanti, Umi Desy Tresnowati Hardi Dewi Masitoh Di Asih I Marudani Di Asih I Maruddani Dwi Ispriyanti Ekananda, Rizki Esti Pratiwi Evi Yulia Handaningrum Fajeriyati, Nurul Fitriah Fitriah Fitriyanti Faruk hartawaty, dheny arina Hasbi Yasin Imam Nur Sholihin Imran, Siskawati Al Juria Ayu Handini Kishatini Kishartini Lambeja, Sulistiawati Liena Sofiana Lilik Ariyanto Manggi, Wildianty M Marna, Syamsul Mawardi Maruddani, Di Asih Maryam Jamilah An Hasibuan Meita Puspa Dewi Mohamad Muklis Muhamad Faliqul Asbah Muhammad Prayito Mulki, Moh Malikul Mustafid Mustafid Nariswari Diwangkari, Nariswari Nashihah, Siti Nunik Nurhasanah Nurina Happy Nurissalma Alivia Putri Octafinnanda Ummu Fairuzdhiya Paramita Indrasari Peratama, Moch Indra Puput Ramadhani R., Ahmad Auliya Rahmat Kartolo Rama Samara Brajawikalpa Rita Rahmawati Rita Rahmawati Rose Debora Julianisa, Rose Debora Rosita, Diah Ayu Rosita, Diah Ayu Rukun Santoso Sabarinah Prasetyo Samsul Bahri Samu, La Ode Sari, Eliya Cicik Puspita Sari, Sasmita Kartika Setya Adi Rahmawan Sihar Tambun Sitti Hadijah, Sitti Sucihatiningsih Dian Wisika Prajanti Sudarno Sudarno Sudarno Sudarno Sukmayanti, Sukmayanti Suparti Suparti Sutikno Sutikno Tahariq, Isra Tarno Tarno Tatik Widiharih Tatik Widiharih Tyas Estiningrum Umar Fauzan Vetranella .T.R.A. Sinaga Waloya, Waloya Wangsa, Arum Kirana Widiantika, Maradella Widya Noviana Noor Winastiti, Lugas Putranti Yuciana Wilandari Yuliana Yuliana Zafirah, Kamilah Zia, Nabila Ghaida Zulfikar, M. Zulkarnain Zulkarnain Zulkarnain, Ahmad Aditya Sidik