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Molecular identification of fungi and the types of toxins produced from contaminated corn grain in Satui, Tanah Bumbu, South Kalimantan, Indonesia Salamiah, Salamiah; Mariana, Mariana; Marsuni, Yusriadi; Pramudi Indar, Muhammad; Sepe, Muslimin; Aphrodyanti, Lyswiana; Abbas, Saipul
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225337-349

Abstract

Fungal contamination in stored corn grain not only reduces grain quality but alsoposes risks to animal and human health due to mycotoxin production. This study highlights the importance of early detection and identification of fungal pathogens in corn as a key aspect of plant protection and postharvest management, as well as the need to determine the types and concentrations of toxins produced. Corn samples were collected from a storage warehouse in Satui Village, Kota Baru Regency, South Kalimantan. Fungal isolation was conducted at the Phytopathology Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Lambung Mangkurat. PCR analysis and gene sequencing were performed at the Genetics Sciences Laboratory, Jakarta, while toxin type and content analyses were carried out at the Animal Husbandry Laboratory, Universitas Gadjah Mada, Yogyakarta. From 11 microbial isolates obtained from corn grain, only one fungal species was identified, namely Aspergillus flavus. This species was found to produce 8.00 ppb of aflatoxin, which remains below the established safety thresholds of 15 ppb for B1 and 20 ppb for total aflatoxins.
Pengaruh Aplikasi Eco-enzyme Untuk Menekan Penyakit Moler Pada Tanaman Bawang Merah (Allium ascalonicum L.) Di Lahan Gambut Anggita, Alwina Ayu; Salamiah, Salamiah; Rosa, Helda Orbani
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2608

Abstract

This research aims to determine the effect of eco-enzyme application in suppressing moler disease in shallot plants (Allium ascalonicum L.) in peatlands. This research used the Completely Randomized Design (CRD) method, where the treatments used in this study consisted of 4 treatments, namely control (without eco-enzyme solution) and 3 treatments of eco-enzyme solution with doses (0.2, 0.6, and 1 mL/200 mL water) which is repeated 5 times. The results of the research showed that the percentage of eco-enzyme that was able to suppress moler disease in treatment t3 (dose 1 mL/200 mL water) was 57.9%, and the smallest percentage of suppression in treatment t1 (dose 0.2 mL/200 mL water) was 43 .6%. In the incubation period research, Fusarium oxysporum appeared for the first time on the 14th day of DAP and occurred in all treatments. Meanwhile, the highest number of tubers/ha was in the control treatment at 58.40 tubers/treatment (162,222 tubers/ha). In the study, the wet weight of tubers in the control treatment had the heaviest tuber wet weight, namely 522.60 tubers/treatment (1,451.7 kg/ha), and the largest tuber diameter was in the treatment given eco-enzyme solution at a dose of 0.6 mL/200 kg. mL of water is 18.3 mm.
Keanekaragaman Mikroba pada Rhizosfer Pertanaman Bawang Merah (Allium ascalonicum L.) yang Diaplikasikan Eco Enzyme di Lahan Gambut Aprilliana, Noor; Marsuni, Yusriadi; Salamiah, Salamiah
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2610

Abstract

This research aims to determine the impact of eco enzyme application on microbial diversity in the rhizosphere of shallot plantations on peatlands. This research used descriptive techniques with a purposive sampling method, consisting of 4 treatments, namely control (without eco enzyme treatment) and 3 treatments of eco enzyme solution with doses (0.2 ml, 0.6 ml, and 1 ml/200 ml water) with 5 repetitions. The identification results showed that there were 65 microbial isolates, of which 28 fungus isolates consisted of 9 fungal genera, namely Trichoderma spp., Penicillium sp., Acremonium sp., Mortierella spp., Humicola sp., Fusarium spp., Aspergillus sp., Culvularia sp. , Pythium spp. and 37 bacterial isolates consisting of 29 gram-positive bacterial isolates and 8 gram-negative bacterial isolates. The results of the research show that the application of eco enzyme has an impact on microbial diversity in the rhizosphere of shallot plantings on peatlands. The diversity of microbial types ranges from 0.9 – 1.4, including in the low – medium category. The richness of microbial species ranges from 0.9 – 1.7, which is included in the low category. The evenness of microbial types ranges between 0.8 – 1.0, including the low category, and the dominance index ranges between 0.3 – 0.5, including the none dominate category.
Efektivitas Tanaman Bintaro (Cerbera manghas) dalam Menekan Serangan Penyakit Moler pada Bawang Merah (Allium ascalonicum L.) di Lahan Gambut Dayatullah, Dayatullah; Salamiah, Salamiah; Aidawati, Noor
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2611

Abstract

Shallots are one of the important vegetable commodities that have been intensively cultivated by farmers for a long time, including in the non-substituted spices group which functions as food flavoring seasonings and ingredients for traditional medicine. In the production process, various constraint were found, one of which is the attack of pathogens that cause moler disease. This study aims to determine the effectiveness of Bintaro solution on the intensity of Moler disease attacks on shallot plants on peatlands. The study was conducted at the Phytopathology Laboratory, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru and in Tegal Arum Village, Landasan Ulin District, Banjarbaru City, South Kalimantan, from June to October 2022. The study used a completely randomized design consisting of 4 treatments and 5 replications. The treatment tested was the application of old b intaro fruit, young bintaro fruit, bintaro leaves plus 1 control treatment. The application of vegetable pesticides was carried out by pouring the solution onto the surface of each shallot plant as much as 5 ml per plant, which was carried out 7 times with an interval of 1 week. Parameters observed were the intensity of the attack of moler disease, fresh weight of the bulbs, the number of bulbs and the diameter of the shallot bulbs. The results showed that the incubation period for the pathogen causing moler disease was 14 days. The application of bintaro plant solution was unable to suppress the attack of moler disease on shallots on peatlands and was unable to increase the number of shallot bulbs, but the application of old bintaro fruit was able to increase tuber wet weight by 41kg/ha and tuber diameter by 2%.
Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Pada Seledri Dengan Bokashi Kipahit Dan Trichoderma sp. Baihaki, Baihaki; Liestiany, Elly; Salamiah, Salamiah
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 3 (2024): Oktober2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i3.2896

Abstract

The celery plant (Apium graveolens L.) is a cultivated leaf vegetable that has advanced capabilities and has a high selling value. Celery also has many properties which are commonly used as decoration and flavoring in cooking. One of the obstacles in increasing celery production is nematode attacks. Nematodes (Meloidogyne spp.) which can affect the number of leaf stalks. The aim of this research was to determine the ability of Bokashi kipahit Plus Trichoderma sp. to reduce attacks by root knot nematodes (Meloidogyne spp.) on celery plants (Apium graveolens L.). The research was designed using a Completely Randomized Design (CRD). What was studied was the impact of giving Bokashi kipahit, Trichoderma sp. and Bokashi kipahit plus Trichoderma sp. The research consisted of four treatments and four replications. The results of the research showed that giving Bokashi kipahit 15.5g/polybag added with Trichoderma sp. 20g/polybag can reduce the NPA population by 48%, and can increase the number of celery stalks by 39%.
Legal review of port management by regional governments from the perspective of regional autonomy Ansori, Fathan; Salamiah, Salamiah
Journal of Law Science Vol. 8 No. 1 (2026): January: Law Science
Publisher : Institute Of computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jls.v8i1.6963

Abstract

Port management occupies a strategic position in supporting transportation systems, trade, and regional development, particularly in an archipelagic state such as Indonesia. Nevertheless, the legal regulation of port management still demonstrates the dominance of Central Government authority, which has the potential to weaken the principle of regional autonomy. This article aims to critically analyze the normative disharmony between lex sectoralis in the port sector and lex generalis in regional government law, as well as its implications for the effectiveness of regional autonomy. This study employs a normative legal research method using statutory, conceptual, and comparative approaches. The findings indicate that the legal construction of port management in Indonesia is not yet fully aligned with the principles of decentralization and the development of international practices that position port authorities as relatively autonomous entities. This article recommends the harmonization of regulations and the reformulation of the division of authority in port management in order to strengthen the role of regional governments without neglecting national interests.
Memburu Burung Manyar (Ploceus Philippinus) Di Persawahan Padi Dengan Memasang Orang-Orangan Sawah Bergerak Otomatis Marsuni, Yusriadi; Salamiah, Salamiah; Mariana, Mariana; Budi, Ismed Setya; Samharinto, Samharinto; Rosa, Helda Orbani; Fitriyanti, Dewi; Pramudi, Muhammad Indar; Pangestu, Prasetyo; Tiwow, Ananda Melko Dayando
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2026): Januari - Juni
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpmittc.v5i1.4127

Abstract

Serangan hama burung manyar (Ploceus philippinus) merupakan permasalahan serius yang dihadapi petani padi sawah di Desa Bentok Darat, Kecamatan Bati-Bati, Kabupaten Tanah Laut. Serangan burung manyar terjadi secara masif pada fase generatif tanaman padi dan menyebabkan kehilangan hasil yang sangat tinggi, bahkan berpotensi mencapai 100%. Kondisi ini semakin meresahkan karena serangan tidak hanya terjadi di satu lokasi, tetapi telah menyebar ke beberapa desa sekitar, seperti Bentok Kampung, Banyu Irang, Bati-Bati, Nusa Indah, dan Benua Raya. Dari total luas tanam padi sekitar 100 hektare, tercatat 10–20 hektare lahan yang siap panen mengalami serangan berat burung manyar sehingga berdampak langsung pada penurunan produksi padi dan pendapatan petani. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan kemampuan kelompok tani dalam mengendalikan serangan burung manyar melalui penerapan teknologi sederhana berupa orang-orangan sawah yang bergerak otomatis dengan memanfaatkan hembusan angin. Metode yang digunakan dalam kegiatan ini meliputi pelatihan dan praktik lapangan yang dilaksanakan dalam lima tahap, yaitu sosialisasi, pembuatan orang-orangan sawah otomatis, pemasangan di lahan persawahan, pengamatan keberadaan sarang burung manyar, serta evaluasi. Kegiatan melibatkan sepuluh orang anggota kelompok tani aktif yang memiliki lahan sawah minimal satu hektare. Sebanyak lima unit orang-orangan sawah otomatis dipasang pada lahan padi yang telah berbuah dan berada di sekitar habitat burung manyar. Evaluasi dilakukan melalui pengamatan harian selama fase berbuah hingga menjelang panen. Hasil kegiatan menunjukkan adanya penurunan intensitas serangan burung manyar pada lahan percontohan serta peningkatan hasil produksi padi, sehingga teknologi orang-orangan sawah otomatis dinilai efektif dan mudah diaplikasikan oleh petani.
Pengamatan Intensitas Serangan Penyakit Gugur Daun Karet di PT Bridgestone Kalimantan Plantation (BSKP) Anisah, Nur; Salamiah, Salamiah; Indar Pramudi, Muhammad
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 9 No. 1 (2026): Edisi 9(1): Februari 2026
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v9i1.3664

Abstract

Leaf fall disease is one of the main problems in rubber plant cultivation (Brazilian rubber tree) which can significantly reduce latex productivity. This study aims to observe the intensity of leaf fall disease attacks on rubber plants treated with organic fertilizer plus Trichoderma, Plant Growth Promoting Rhizobacteria (PGPR), and eco-enzymes. Observations were conducted three times in the rubber plantation owned by PT. Bridgestone Kalimantan Plantation (BSKP), using a visual assessment method for disease symptoms and calculating the percentage of infected leaves. The data obtained were analyzed descriptively and quantitatively to determine disease progression trends. The results showed that the application of organic fertilizer plus PGPR was able to suppress disease attacks by 74.50%. followed by other treatments: application of organic fertilizer plus Trichoderma, control plants without application, and organic fertilizer plus Trichoderma supplemented with eco-enzyme, with percentages of attack suppression of 68.97%, 68.83%, and 66.83%, respectively. Air humidity and daily rainfall factors showed a positive correlation with increasing attack intensity. These findings demonstrate the importance of environmentally friendly disease control strategies. This research is expected to form the basis for more effective and sustainable management of leaf fall disease.
Efektifitas Beberapa Spesies Trichoderma spp. Terhadap Keparahan Penyakit TanamaniSeledri (Apium graveolensiL.) YangiTerinfeksi Nematoda Puru Akar (Meloidogyne spp.) Muhammad hedir Ali; Fitriyanti, Dewi; Salamiah, Salamiah
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 9 No. 1 (2026): Edisi 9(1): Februari 2026
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v9i1.3670

Abstract

Celery plants have many benefits, one of which is being used as an additional ingredient in cooking or as a food flavoring. Apart from being rich in benefits, celery plants also have quite promising economic value. The decline in celery production in Indonesia is also caused by root knot nematodes, Meloidogyne spp. Is one of the important pathogens in various horticultural crops and some food crops in Indonesia. Environmentally friendly pathogen control necessary done using biological agents, Trichoderma sp. is a saprophytic soil microorganism that naturally attacks pathogenic fungi and is beneficial for plants, a mechanism carried out by antagonistic agents. Trichoderma sp. against pathogens are mycoparasites and antibiotics. This study aims to determine the effectiveness of several species Trichoderma spp. in reducing nematode populations pure root (Meloidogyne spp.) on celery plants. Research This applies the Design method complete Random, which aims to obtain accurate data regarding the impact of attacks, which involves one factor, namely treatment using 500 nematode eggs as a control (P0) and using three treatments, namely (P1) = 500 nematode eggs (Meloidogyne spp.) + Trichodermaspp. from bamboo roots; (P2) = 500 nematode eggs (Meloidogyne spp.) + Trichodermaspp. from chili roots; (P3) = 500 nematode eggs (Meloidogyne spp.) + Trichodermaspp. from elephant grass roots. The results of the study showed that the treatmentTrichodermaspp. from elephant grass roots (P3) reduced disease severity by an average of 20.5% and a maximum of 61.5% (P0). The lowest root knot nematode population was 170.3 individuals (P3), while the highest population was found in the control treatment (P0), with 505.1 NPA individuals.
Effect of Storage Medium Composition on the Viability of Antagonistic Bacteria Against Stem Rot Disease Hidayati, Nurul; Salamiah, Salamiah; Wahdah, Raihani; Razie, Fahrur; Arfianto, Fahruddin
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol. 14 No. 1: January 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v14i1.18727

Abstract

Stem rot is a major constraint in sweet corn and maize production, especially under humid conditions that favor pathogen development. Although antagonistic bacteria offer a sustainable alternative to chemical control, their field use is often limited by viability loss during room-temperature storage, making carrier formulation critical for shelf-life and product reliability. This study evaluated the effect of biochar–peat carrier composition on the storage viability of five antagonistic bacterial isolates for stem rot management in sweet corn. A two-factor factorial experiment (4 carrier compositions × 5 isolates) with three replications (60 experimental units) was conducted under laboratory conditions. Carriers were prepared as biochar: peat mixtures (v/v) of 3:1, 1:1, 1:3, and 100% peat. Each carrier unit (10 g) was inoculated with 1 mL bacterial suspension (≈10⁸ cfu mL⁻¹), sealed, and stored at room temperature (28–29 °C). Viability was quantified at 30, 60, and 90 days after inoculation (DAI) using serial dilution and plate counts on TSA, expressed as cfu g⁻¹. Data at 60 DAI were analyzed by factorial ANOVA including block (Group), medium (M), isolate (P), and M×P effects, followed by LSD (5%) for mean separation using SPSS 25. At 30 DAI, bacterial densities were high and did not differ among carriers, indicating comparable short-term support across media. At 60 DAI, carrier composition significantly affected bacterial density, whereas isolate and M×P interaction effects were not significant, indicating a general carrier-driven response across isolates. The biochar-rich carrier (3:1) maintained the highest mean population (8.844 × 10⁷ cfu g⁻¹). By 90 DAI, all treatments declined, yet the 3:1 carrier retained the highest density (1.089 × 10⁷ cfu g⁻¹). Overall, biochar-enriched carriers, particularly the 3:1 biochar: peat mixture, better preserved antagonistic bacterial viability under non-refrigerated storage up to 90 days.
Co-Authors Abda Abda Abdullah Fadily Ahmad Reza Syahputra Matondang Anak Agung Istri Sri Wiadnyani Anggita, Alwina Ayu Anisah, Nur Anshari, Ahmad Aprilliana, Noor Arnida Arnida Aulia Ghanisa, Puspa Baihaki Baihaki Bersinar, Saras Dayatullah, Dayatullah Dewi Fitriyanti Dewi Fitriyanti, Dewi Dicky Andiarsa Eko Aprianto Eko Suhartono Emeliawati Emeliawati Evi Mintowati, Evi Fahruddin Arfianto Fathan Ansori Fauziyah, Nur Feri Arianto Fikasari, Devaliana catria Hakimah Halim Helda Orbani Rosa Ilhamiyah Ilhamiyah Ineu Sulastrini Ismed Setya Budi Liestiana Indriyati Liestiany, Elly Liling Triyasmono Luis, Pankrasius Ivan Lutfhi Fatah Lyswiana Aphrodyanti M. Hidayat, Iteu Magfirah, Asmaul Mariana Mariana Mariana Mariana Mariana Mariana Marsuni, Yusriadi Meliana Elvianita Muhammad hedir Ali Muhammad Ikhwan Rizki Muhammad Indar Pramudi Muhammad Rasyid Ridha Muhammad Redho Muthia Septarina MUTHIA SEPTARINA Nahdhah Nahdhah NINGRUM AMBARSARI Ningrum, Syadiyah Dwi NOOR AIDAWATI Noor Herda Suriyani Nurul Hidayati nurul latifah Pangestu, Prasetyo Pramudi Indar, Muhammad Pramudyani, Lelya Raihani Wahdah Razie, Fahrur REFLINUR REFLINUR Reno Julian Putera Nalu Rina Lestari Rosalina Kumalawati SAFITRI, YUNIEKA AULIA Saipul Abbas Samharinto Samharinto Santi Suminar Sepe, Muslimin Simamora, Khairul Huda Sutomo Sutomo syaifuddin syaifuddin Syamsiar, Syamsiar Tarigan, Divayona Begidir Br Tarigan Tiara Anggraini Timur Priono Tiwow, Ananda Melko Dayando Tri Handayani Waluyo, Nurmalita Winda Rizky Oktaviani Zairin Zairin