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Molecular identification of fungi and the types of toxins produced from contaminated corn grain in Satui, Tanah Bumbu, South Kalimantan, Indonesia Salamiah, Salamiah; Mariana, Mariana; Marsuni, Yusriadi; Pramudi Indar, Muhammad; Sepe, Muslimin; Aphrodyanti, Lyswiana; Abbas, Saipul
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225337-349

Abstract

Fungal contamination in stored corn grain not only reduces grain quality but alsoposes risks to animal and human health due to mycotoxin production. This study highlights the importance of early detection and identification of fungal pathogens in corn as a key aspect of plant protection and postharvest management, as well as the need to determine the types and concentrations of toxins produced. Corn samples were collected from a storage warehouse in Satui Village, Kota Baru Regency, South Kalimantan. Fungal isolation was conducted at the Phytopathology Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Lambung Mangkurat. PCR analysis and gene sequencing were performed at the Genetics Sciences Laboratory, Jakarta, while toxin type and content analyses were carried out at the Animal Husbandry Laboratory, Universitas Gadjah Mada, Yogyakarta. From 11 microbial isolates obtained from corn grain, only one fungal species was identified, namely Aspergillus flavus. This species was found to produce 8.00 ppb of aflatoxin, which remains below the established safety thresholds of 15 ppb for B1 and 20 ppb for total aflatoxins.
Pengaruh Aplikasi Eco-enzyme Untuk Menekan Penyakit Moler Pada Tanaman Bawang Merah (Allium ascalonicum L.) Di Lahan Gambut Anggita, Alwina Ayu; Salamiah, Salamiah; Rosa, Helda Orbani
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2608

Abstract

This research aims to determine the effect of eco-enzyme application in suppressing moler disease in shallot plants (Allium ascalonicum L.) in peatlands. This research used the Completely Randomized Design (CRD) method, where the treatments used in this study consisted of 4 treatments, namely control (without eco-enzyme solution) and 3 treatments of eco-enzyme solution with doses (0.2, 0.6, and 1 mL/200 mL water) which is repeated 5 times. The results of the research showed that the percentage of eco-enzyme that was able to suppress moler disease in treatment t3 (dose 1 mL/200 mL water) was 57.9%, and the smallest percentage of suppression in treatment t1 (dose 0.2 mL/200 mL water) was 43 .6%. In the incubation period research, Fusarium oxysporum appeared for the first time on the 14th day of DAP and occurred in all treatments. Meanwhile, the highest number of tubers/ha was in the control treatment at 58.40 tubers/treatment (162,222 tubers/ha). In the study, the wet weight of tubers in the control treatment had the heaviest tuber wet weight, namely 522.60 tubers/treatment (1,451.7 kg/ha), and the largest tuber diameter was in the treatment given eco-enzyme solution at a dose of 0.6 mL/200 kg. mL of water is 18.3 mm.
Keanekaragaman Mikroba pada Rhizosfer Pertanaman Bawang Merah (Allium ascalonicum L.) yang Diaplikasikan Eco Enzyme di Lahan Gambut Aprilliana, Noor; Marsuni, Yusriadi; Salamiah, Salamiah
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2610

Abstract

This research aims to determine the impact of eco enzyme application on microbial diversity in the rhizosphere of shallot plantations on peatlands. This research used descriptive techniques with a purposive sampling method, consisting of 4 treatments, namely control (without eco enzyme treatment) and 3 treatments of eco enzyme solution with doses (0.2 ml, 0.6 ml, and 1 ml/200 ml water) with 5 repetitions. The identification results showed that there were 65 microbial isolates, of which 28 fungus isolates consisted of 9 fungal genera, namely Trichoderma spp., Penicillium sp., Acremonium sp., Mortierella spp., Humicola sp., Fusarium spp., Aspergillus sp., Culvularia sp. , Pythium spp. and 37 bacterial isolates consisting of 29 gram-positive bacterial isolates and 8 gram-negative bacterial isolates. The results of the research show that the application of eco enzyme has an impact on microbial diversity in the rhizosphere of shallot plantings on peatlands. The diversity of microbial types ranges from 0.9 – 1.4, including in the low – medium category. The richness of microbial species ranges from 0.9 – 1.7, which is included in the low category. The evenness of microbial types ranges between 0.8 – 1.0, including the low category, and the dominance index ranges between 0.3 – 0.5, including the none dominate category.
Efektivitas Tanaman Bintaro (Cerbera manghas) dalam Menekan Serangan Penyakit Moler pada Bawang Merah (Allium ascalonicum L.) di Lahan Gambut Dayatullah, Dayatullah; Salamiah, Salamiah; Aidawati, Noor
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 2 (2024): Vol 7(2): Juni 2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i2.2611

Abstract

Shallots are one of the important vegetable commodities that have been intensively cultivated by farmers for a long time, including in the non-substituted spices group which functions as food flavoring seasonings and ingredients for traditional medicine. In the production process, various constraint were found, one of which is the attack of pathogens that cause moler disease. This study aims to determine the effectiveness of Bintaro solution on the intensity of Moler disease attacks on shallot plants on peatlands. The study was conducted at the Phytopathology Laboratory, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru and in Tegal Arum Village, Landasan Ulin District, Banjarbaru City, South Kalimantan, from June to October 2022. The study used a completely randomized design consisting of 4 treatments and 5 replications. The treatment tested was the application of old b intaro fruit, young bintaro fruit, bintaro leaves plus 1 control treatment. The application of vegetable pesticides was carried out by pouring the solution onto the surface of each shallot plant as much as 5 ml per plant, which was carried out 7 times with an interval of 1 week. Parameters observed were the intensity of the attack of moler disease, fresh weight of the bulbs, the number of bulbs and the diameter of the shallot bulbs. The results showed that the incubation period for the pathogen causing moler disease was 14 days. The application of bintaro plant solution was unable to suppress the attack of moler disease on shallots on peatlands and was unable to increase the number of shallot bulbs, but the application of old bintaro fruit was able to increase tuber wet weight by 41kg/ha and tuber diameter by 2%.
Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Pada Seledri Dengan Bokashi Kipahit Dan Trichoderma sp. Baihaki, Baihaki; Liestiany, Elly; Salamiah, Salamiah
JURNAL PROTEKSI TANAMAN TROPIKA Vol. 7 No. 3 (2024): Oktober2024
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jptt.v7i3.2896

Abstract

The celery plant (Apium graveolens L.) is a cultivated leaf vegetable that has advanced capabilities and has a high selling value. Celery also has many properties which are commonly used as decoration and flavoring in cooking. One of the obstacles in increasing celery production is nematode attacks. Nematodes (Meloidogyne spp.) which can affect the number of leaf stalks. The aim of this research was to determine the ability of Bokashi kipahit Plus Trichoderma sp. to reduce attacks by root knot nematodes (Meloidogyne spp.) on celery plants (Apium graveolens L.). The research was designed using a Completely Randomized Design (CRD). What was studied was the impact of giving Bokashi kipahit, Trichoderma sp. and Bokashi kipahit plus Trichoderma sp. The research consisted of four treatments and four replications. The results of the research showed that giving Bokashi kipahit 15.5g/polybag added with Trichoderma sp. 20g/polybag can reduce the NPA population by 48%, and can increase the number of celery stalks by 39%.