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Motilitas dan Viabilitas Spermatozoa Sapi Rambon di Desa Kemiren Banyuwangi Prastika, Zeni; Susilowati, Suherni; Agustono, Bodhi; Safitri, Erma; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.103 KB) | DOI: 10.20473/jmv.vol1.iss2.2018.38-42

Abstract

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermatozoa adalah salah satu indikator fertilitas spermatozoa dan kualitas semen. Penelitain ini adalah penelitian deskriptif. Penelitian ini bertujuan untuk mengetahui motilitas dan viabilitas semen segar sapi Rambon di Desa Kemiren Kecamatan Glagah Kabupaten Banyuwangi. Semen pada penelitian ini di tampung menggunakan vagina buatan dari 6 ekor sapi Rambon jantan. Pemeriksaan motilitas spermatozoa dan viabilitas spermatozoa dilakukan di klinik Linawati Banyuwangi. Hasil pada penelitian ini disajikan dalam bentuk tabel. Parameter penelitian ini adalah motilitas dan viabilitas spermatozoa sapi Rambon. Hasil pemeriksaan motilitas spermatozoa sapi Rambon 83,3±4,1% dan viabilitas spermatozoa sapi Rambon 86,7±2,3%. Kesimpulan dari penelitian menunjukan bahwa motilitas dan viabilitas spermatozoa sapi Rambon baik dan memenuhi syarat semen beku untuk inseminasi buatan.
Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Proliferasi Lapisan Endometrium Mencit (Mus musculus) Salahuddin, Muhammad Soleh; Safitri, Erma; Yunita, Maya Nurwartanti; Susilowati, Suherni; Hamid, Iwan Sahrial; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.497 KB) | DOI: 10.20473/jmv.vol2.iss1.2019.49-54

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak kedelai untuk meningkatan proliferasi lapisan endometrium dalam proses perkembangan organ uterus pada mencit (Mus musculus) betina. Kelompok kontrol (K) tidak diberi perlukaan. Kelompok P1 diberi ekstrak kedelai dengan dosis 12,5mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 25mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 37,5mg/kgBB. Kelompok P4 diberi ekstrak kedelai dengan dosis 50mg/kgBB. Perlakuan dilakukan setiap hari sekali selama empat belas hari. Hasil uji One Way Anova menunjukkan perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Ketebalan endometrium kelompok P2, P3, dan P4 berbeda secara nyata (p<0,05), dengan kelompok kontrol (K), dan kelompok kontrol (K) tidak berbeda nyata dengan kelompok P1 (p>0,05), serta kelompok P1 tidak berbeda nyata dengan kelompok P2 (p>0,05). Kandungan senyawa fitoestrogen yaitu isoflavon berupa daidzein dan genistein bekerjasama dalam mengoptimalkan proliferasi lapisan endometrium pada uterus mencit (Mus musculus) betina. Daidzein bekerja sebagai senyawa estrogenik untuk berikatan dengan reseptor estrogen pada uterus dan genistein bekerja sebagai penghambat pertumbuhan lapisan endometrium yang berlebih pada jumlah tertentu. Kesimpulan dari penelitian ini adalah ekstrak kedelai (Glycine max) efektif untuk peningkatan proliferasi lapisan endometrium pada uterus mencit betina.
Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris Prayogo, Danu; Susilowati, Suherni; Prastiya, Ragil Angga; Safitri, Erma; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.188-195

Abstract

This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.
Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years Purnawan, Ahmad Budi; Rimayanti, Rimayanti; Susilowati, Suherni; Mustofa, Imam; Hernawati, Tatik; Safitri, Erma
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.162-172

Abstract

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.
Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Anugrah, Alvyan Lantang; Eliyani, Hana; Utomo, Budi; Susilowati, Suherni; Mafruchati, Maslichah; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

Abstract

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.
Pemberdayaan Kelompok Asuhan Mandiri Manggala melalui Pemanfataan Tanaman Toga dengan Pupuk Organik Koestanti S, Emy; Suprihati , Endang; Sukartini, Tintin; Hidanah, Sri; Paramita Lokapirnasari, Widya; Susilowati, Suherni; Maslachah, Lilik; Angelina Hendarti, Gracia
Aksiologiya: Jurnal Pengabdian Kepada Masyarakat Vol 8 No 4 (2024): November
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/aks.v8i4.24107

Abstract

Tanaman Obat Keluarga (TOGA) merupakan jenis tanaman yang memiliki dampak positif secara farmakologis pada tubuh manusia dan umumnya ditanam baik di lingkungan rumah tangga maupun dalam skala komunal. Penelitian ilmiah terhadap tanaman obat keluarga yang memiliki khasiat pengobatan juga mengungkapkan bahwa tanaman tersebut mengandung zat-zat atau senyawa aktif yang terbukti memberikan manfaat bagi kesehatan. Fokus pengabdian masyarakat, meningkatkan pengetahuan dan ketrampilan pada kelompok asuhan mandiri tentang aplikasi pupuk organik sebagai media untuk Taman Toga yang ramah lingkungan dan aplikasi pembuatan jejamuan metode ekstraksi untuk mengeluarkan zat aktif pada tanaman herbal. Hasil evaluasi menunjukkan 100% peserta menanam temulawak, sebanyak 35 peserta (81%) menanam temulawak dan kunir sebanyak 25 peserta menanam temulawak, kunir dan sambiloto (58%). Pengetahuan tentang pupuk organik dari peserta yaitu tidak tahu sebanyak 4 peserta (9,5%), cukup tahu sebanyak 25 peserta (58%) dan sangat tahu sebanyak 14 peserta (32,5%). Sedangkan pemahaman tentang pemanfaatkan pupuk organic terhadap toga didapatkan hasil 29 peserta cukup paham (67,5%), 8 peserta sangat paham (18,5%) dan 5 peserta masih belum paham (12%). Permasalahan tanaman obat pada kelompok asuhan mandiri dapat diatasi dengan cara aplikasi pupuk organik berasal dari kotoran hewan domba yang telah diolah menjadi pupuk yang ramah lingkungan.
Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Efisiensi Produksi Melalui Penerapan Teknologi Reproduksi Inseminasi Buatan Pada Kawin Silang Kambing Poteh Di Desa Baipajung Kecamatan Tanah Merah Kabupaten Bangkalan Susilowati, Suherni; Mustofa, Imam; Wahyu Suprayogi, Tri; Supriyadi; Mulyati, Sri
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2024): Desember 2024
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v6i2.372

Abstract

Baipajung is a village with quite good potential value and needs to be developed in the agriculture, plantation and livestock sectors. Generally, in the village goats are mated naturally, namely one cage is used for 1 male goat and 3 female goats. The population of goats in Baipajung village is 488 male goats, 878 female goats and 587 goat kids, so with the livestock capital it can be used as a source of community income. The problem that occurs in Baipajung village, although the goat population is quite large, their maintenance is only traditional, just a side business as rojokoyo or as savings if they don't have money. Goat farmers want their goats after giving birth to have superior characteristics such as increased percentage of offspring, heavier weaning weight, higher quality goats or more efficient use of feed resources. The efficiency of livestock production can be increased through the application of Artificial Insemination reproductive technology in crossbreeding Poteh or local goats with Boar goat studs.
Ekonomi Kreatif Pakan Tanpa Hijauan dan Inovasi Olahan Susu Menuju Wisata Sapi Perah Mulyati, Sri; Mustofa, Imam; Rimayanti, Rimayanti; Susilowati, Suherni; Supriyadi, Supriyadi
Smart Dedication: Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2025): Smart Dedication: Jurnal Pengabdian Masyarkat
Publisher : SMART SCIENTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70427/smartdedication.v2i1.157

Abstract

Peternakan sapi perah memegang peran penting dalam mendukung kebutuhan pangan nasional, khususnya dalam penyediaan susu sebagai sumber protein hewani. Namun, sektor ini menghadapi sejumlah tantangan signifikan yang menghambat perkembangan dan keberlanjutannya. Ketergantungan pada hijauan sebagai pakan utama sering kali menjadi kendala, terutama ketika musim kemarau atau keterbatasan lahan hijauan mengurangi pasokan. Pentingnya ekonomi kreatif dalam pengembangan peternakan sapi perah, dengan fokus pada inovasi pakan tanpa hijauan, diversifikasi produk olahan susu, dan konsep wisata sapi perah sebagai strategi untuk meningkatkan nilai tambah dan daya saing sektor peternakan. Kegiatan ini bekerjasama dengan KUD “Tani Wilis dan UMKM” Wisata Sapi Perah”. Keberhasilan program ini dilakukan pendidikan dan pelatihan tentang pakan tanpa hijauan dan inovasi olahan susu. Pendekatan ekonomi kreatif melalui inovasi pakan tanpa hijauan dan pengembangan produk olahan susu memiliki potensi besar untuk meningkatkan keberlanjutan peternakan sapi perah, terutama di wilayah pedesaan seperti Desa Sendang. Inovasi ini tidak hanya membantu peternak menghadapi tantangan ketersediaan pakan hijauan selama musim kemarau, tetapi juga membuka peluang untuk diversifikasi produk yang bernilai tambah, seperti yoghurt, keju, dan es krim. Integrasi antara inovasi peternakan dan konsep wisata edukasi berbasis sapi perah menciptakan peluang ekonomi baru melalui wisata sapi perah. Konsep ini memberikan manfaat berlapis: dalam meningkatkan pendapatan peternak, memperkenalkan masyarakat luas pada dunia peternakan, serta memperkuat daya tarik wisata lokal. Keberhasilan pendekatan ini ditunjukkan oleh penerimaan yang baik dari masyarakat setelah pelatihan, di mana hasil evaluasi menunjukkan rata-rata tingkat pemahaman sebelum pelatihan hanya mencapai sekitar 50%-60% sedangkan setelah pelatihan terjadi peningkatan signifikan sebesar 100% dalam pengetahuan dan keterampilan.
Co-Authors Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra Budi Utomo BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri ERMA SAFITRI Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hariadi, Mas'ud Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas'ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanti Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti