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All Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Berkala Penelitian Hayati Jurnal Kedokteran Hewan
Suherni Susilowati
Department Of Veterinary Reproduction - Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures Lucky Diba; Suzanita Utama; Tjuk Imam Restiadi; Suherni Susilowati; Nusdianto Triakoso; Dwi Wijayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.81-89

Abstract

The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was collected through massage in the morning. The pooled semen sample was divided into four treatment groups. In the T0 group the semen was diluted in 0.9% NaCl, while in the T1, T2 and T3 groups the semen was diluted in 0.9% NaCl added with 5, 10 and 15% egg yolk. The results showed that the motility and viability of spermatozoa decreased when stored at cool temperatures for ten hours (p <0.05). Semen of roosters stored at 5°C in saline solution without the addition of egg yolks showed the lowest motility and viability of spermatozoa (p <0.05). The addition of egg yolk into the saline extender increased the motility and viability of spermatozoa. Concentration of 15% egg yolk in saline solution resulted in the highest spermatozoa motility and viability when stored for up to 8 hours (p <0.05). However, motility and viability of spermatozoa at 10 hours of storage were not significantly different (p >0.05) with the addition of 10% and 15% egg yolks. Therefore, it could be concluded that the addition of 15% egg yolk into a saline solution as an extender could maintain the motility and viability of kampung rooster spermatozoa when stored at 5°C for 10 hours.
PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Suherni Susilowati; Tatik Hernawati
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.363

Abstract

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untuk meningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukan aplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.
Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C Ardina Sahra Miranda; Tri Wahyu Suprayogi; Budi Utomo; Suherni Susilowati; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.148-156

Abstract

Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-GEY) may be a good cryoprotectants due to their antioxidant properties. This study aimed to determine the effect of adding GTLE to the SM-GEY extender on the quality of Sapudi ram spermatozoa stored at 5°C. The fresh semen sample was divided into four different GTLE treatment groups, which each contained a 0.1 mL semen sample and a 25-mL extender of SM-GEY. Group T0: no GTLE added to SM-GEY; Groups T1, T2, and T3: 0.1 mL semen diluted in 25 mL SM-GEY with 0.05, 0.10, and 0.15 mg GTLE. Extended semen was then stored at 5°C, and its quality was evaluated daily for five days. The variables observed included spermatozoa motility, viability, and membrane integrity. Data were analyzed using one-way analysis of variance followed by Duncan's test using Statistical Program and Service Solution version 23. The result of this study was that adding 0.05 mg GTLE to 25 mL of SM-GEY extender significantly maintained the spermatozoa motility, viability, and plasma membrane integrity of Sapudi ram spermatozoa for three days at 5°C (p <0.05). Therefore, it could be concluded that adding 0.05 mg of GTLE to the SM-GEY extender preserved Sapudi ram spermatozoa's motility, viability, and membrane integrity for three days at 5°C.
Co-Authors Abrian Panggalih Indra Pratama Ardina Sahra Miranda Ayun Tria Marga Retta Budi Utomo Dinda Galuh Pitaloka Dwi Wijayanti Eka Pramyrtha Hestianah Fania Selfiannisa Hana Eliyani Hermin Ratnani Kukuh Prastyaningtyas Kusnoto Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lucky Diba Mas’ud Hariadi Nusdianto Triakoso Poedji Hastutiek Rahmi Sugihartuti Restiadi, Tjuk Imam Retno Sri Wahjuni Rochmah Kurnijasanti Samuel Inioluwa Akeju Shinta Putri Quraini Sri Chusniati Sri Pantja Madyawati Sunarso, Agus Suzanita Utama Tatik Hernawati Tjuk Imam Restiadi Tri Wahyu Suprayogi Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yossy Imam Candika