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All Journal Jurnal Keperawatan Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biology, Medicine, & Natural Product Chemistry The Indonesian Biomedical Journal Journal of Islamic Medicine Abdimasku : Jurnal Pengabdian Masyarakat Heart Science Journal JSMARTech : Journal of Smart Bioprospecting and Technology Malang Respiratory Journal Cermin Dunia Kedokteran
Djanggan Sargowo
Pascasarjana Fakultas Kedokteran, Universitas Brawijaya, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : The Indonesian Biomedical Journal

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Vegetables Spices Fermentation Extract Inhibit NAFLD Development by Attenuation of Steatosis through Suppression of C/EBPα and TNFα mRNA Expression Rachmawati, Ermin; Machdaniar, Maulidya; Kinasih, Larasati Sekar; Sargowo, Djanggan; Syarifuddin, Brillian Putra; Putri, Fayza Cinta Rahmadina; Ismail, Mahrus
The Indonesian Biomedical Journal Vol 16, No 2 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i2.2934

Abstract

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD), characterized by inflammation and steatosis, is one of the metabolic problems that trigger serious complications. Statin is usually used for NAFLD therapy, however, statin intolerance and resistance reduce the effectiveness of NAFLD treatment. Therefore, this study was conducted to develop the phytopharmaca against NAFLD by elucidating the anti-inflammatory and steatosis suppression of vegetable spices fermentation extract (VSFE) in rabbits induced with high-fat diet (HFD).METHODS: Twenty New Zealand White rabbits were determined into 4 groups; which were groups receiving standard diet (SD), HFD, HFD and 100 mg/kgBW VSFE, as well as HFD and 200 mg/kgBW VSFE. mRNA expressions of tumor necrosis factor alpha (TNFα) and CCAAT/enhancer-binding protein alpha (C/EBPα) were evaluated. The TNFα concentration was quantified after protein and RNA isolation. Inflammation and steatosis were observed in liver hematoxylin-eosin-stained preparate using microscope.RESULTS: Macrophage cells and hepatic steatosis showed significant decrease in a drug dependent manner. An effective dose of 200 mg/kgBW decreased C/EBPα and TNFα expression to 0.45±0.32 and 0.72±0.29 (p=0.013 and p=0.002, compared to the HFD group), respectively. TNFα inflammatory cytokine concentrations also responded to the administration of 100 mg/kgBW and 200 mg/kgBW doses, with the value of 31.72±10.40 mg/dL and 48.35±7.15 mg/dL (p=0.009 and p=0.002, compared to the HFD group), respectively.CONCLUSION: VSFE might prevent NAFLD by inhibit steatosis and inflammation through suppression of C/EBPα expression and TNFα expression.KEYWORDS: C/EBPα, TNFα, fermentation, steatosis, NAFLD
Expression of Plasma miRNA-133a is Significantly Lower in Acute Coronary Syndrome (ACS) than in Healthy/Non-ACS Subjects Rachmawati, Ermin; Sargowo, Djanggan; Saputra, Indra Wahyu; Riskiyah, Riskiyah; Handirosiyanto, Ikhwan; Hakim, Arief Rachman; Ismail, Mahrus; Tarsadi, Tarsadi; Maulana, Syafiq; Ahdi, Iwal Reza; Puspitasari, Alvina; Wardhani, Syanindita Puspa
The Indonesian Biomedical Journal Vol 16, No 5 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i5.3243

Abstract

BACKGROUND: The current biomarker diagnostic modality for acute coronary syndrome (ACS), cardiac troponin, has several limitations. Emerging studies showed that micro-RNA (miR)-133a was released from infarcted heart to circulation, yet the diagnostic value of miR-133a in ACS demonstrated a conflicting result. Therefore, this study was conducted to investigate the potency of plasma miR-133a as a biomarker candidate of ACS.METHODS: This was a case-controlled study, involving ACS and control subjects. The sociodemographic and clinical characteristics were assessed through medical records. A final of 39 ACS and 31 control subjects (consist of healthy and non-ACS subjects) passed the selection procedure by demonstrating a high purity of RNA. miR-133a from ACS and control subjects were detected by quantitative polymerase chain reaction (qPCR). Expression of miR-133a was evaluated for sensitivity and specificity as an ACS biomarker diagnostic using the receiver operating characteristic (ROC) curve.RESULTS: Plasma miR-133a expression was stably found in ACS subjects. The plasma miR-133a level was lower in ACS than in control subjects. miR-133a effectively distinguished ACS subjects from healthy subjects (AUC=0.911) and exhibited high diagnostic performance, with a sensitivity of 87.1% and specificity of 100% at a cut-off value of 44.035. In an extended model including both control subjects (healthy and non-ACS with comorbid conditions), miR-133a maintained diagnostic significance (AUC=0.874), showing sensitivity of 76.9% and specificity of 100% at a cut-off value of 11.69.CONCLUSION: Plasma miR-133a is significantly lower and effectively distinguishes ACS patients from both healthy individuals and non-ACS individuals with comorbid, with a cut-off value of 11.69. Therefore, plasma miR-133a is suggested to be a good candidate for diagnostic biomarkers of ACS.KEYWORDS: circulating miRNA, miRNA-133a, acute coronary syndrome, diagnostic biomarker
Co-Authors -, Vidyawati Abdillah Iskandar Achdiat Agoes Ahdi, Iwal Reza Andri Andri Anjarwani, Setyasih Arief Rachman Hakim Aryanugraha, Teguh Askandar Tjokroprawiro Cholid Tri Tjahjono Dwi Sarbini Edi Widjajanto Elvira Sari Dewi Endah Kusuma Rastini Erawati, Dini Rachma Ermin Rachmawati Galih Prakosa, Ardani Galuh, Lukitasari Ayu Handirosiyanto, Ikhwan I Ketut Gede Muliartha Ismail, Mahrus Ivan Setiawan Kurnianingsih, Novi Lalu Aries Fahrozi, Lalu Larasati Sekar Kinasih Machdaniar, Maulidya Maulana, Syafiq Mohammad Saifur Rohman Parsama Putra, Ngakan Putu Puspitarini, Marinda Dwi Puspitasari, Alvina Putri, Fayza Cinta Rahmadina Rahimah, Anna Fuji Retnani, Diah Prabawati Rias Gesang Kinanti Rifa'i, Muhaimin Rifa’i, Muhaimin riskiyah, Riskiyah - Rizal, Ardian Saputra, Indra Wahyu Saputri, Diana Nanda Saskia Dyah Handari Satrijo, Budi Satuman Satuman Satwikajati, Sawitri Sidarta, Erdo Puncak Sri Andarini Suprayoga, Imam Mi'raj Syarifuddin, Brillian Putra Tarsadi, Tarsadi Teuku Heriansyah, Teuku Titi A W Titin A Wihastuti, Titin A Titin Andri Wihastuti Tony Suharsono, Tony Totowiputro, Dicky Kurniawan Viantry, Paramita Viera Wardhani Wardhani, Syanindita Puspa Widodo Widodo Yurista, Salva Reverentia