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All Journal Universa Medicina Majalah Kesehatan Pharmamedika Proceeding ISETH (International Summit on Science, Technology, and Humanity) Info Abdi Cendekia Junior Medical Journal Jurnal Pengabdian Masyarakat Kesehatan Gigi FOKGII
Wening Sari
Yarsi University
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Journal : Universa Medicina

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Ethanol extract of Abrus precatorius L. leaves diminishes inflammatory responses in nicotine-treated human gingival fibroblasts: an in vitro study Kesumaningtias, Raden Roro Widorini; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 3 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.272-279

Abstract

BACKGROUNDNicotine induces oxidative stress in human gingival fibroblasts (HGF) and stimulates the production of cytokines that trigger inflammation. Abrus precatorius L. (AP) leaves contain antioxidants with anti-inflammatory properties that can prevent the formation of free radicals and reduce tissue damage due to inflammation. This study aimed to determine the effect of ethanolic extract of AP leaves (EAP) on interleukin (IL-6) levels and cyclooxygenase-2 (COX-2) gene expression in gingival fibroblasts exposed to nicotine. METHODSCells were randomized into six treatment groups and clustered into the non-treatment control group (NTC), solvent control (SC), nicotine control (NC), and groups treated with nicotine and EAP at doses of 9.375 µg/mL, 18.75 µg/mL, and 37.5 µg/mL, respectively, for 24 hours. IL-6 levels were examined using the Elisa method, while COX-2 gene expression was assessed using PCR. Data were analyzed using Oneway ANOVA and the Kruskal Wallis test. RESULTSIL-6 levels and COX-2 expression were considerably higher in the nicotine control group. Conversely, the cell groups treated with nicotine and EAP had substantially decreased levels of both inflammatory markers IL-6 and COX-2 (p=0.029) across all EAP dose levels compared to the nicotine control group. The highest reduction in response was observed at the dose of 9.375 ìg/mL EAP. CONCLUSIONThese results highlight the potential of Abrus precatorius L. in relieving nicotine-induced inflammation in smokers. By suppressing the production of inflammatory mediators IL-6 and COX-2 in HGF, EAP presents a promising avenue for further in vitro research.
Nicotine reduces cell viability and induces oxidative stress in human gingival fibroblasts Azmi, Sabrina; Hadi, Restu Syamsul; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 1 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.20-30

Abstract

BackgroundNicotine, as the main component of cigarettes, is known to interfere with the proliferation of human gingival fibroblasts (HGFs) and can trigger oxidative stress. This study aimed to analyze the impact of nicotine on viability, expression of the antioxidant Nrf2, levels of the product of oxidative stress malondialdehyde (MDA), and the migration capacity of HGFs. MethodsAn experimental laboratory study used fibroblasts isolated from healthy human gingiva. The cells were grouped into the non-treatment control group (NTC), the solvent control (SC), and the treatment groups, exposed to nicotine at various concentrations for twenty-four hours. Cell viability was assesed using the cell counting kit-8 (CCK-8), Nrf2 expression was examined using ELISA, MDA level was measured using an MDA kit, and migration capacity was assessed using a scratch assay. Statistical analysis used one-way Anova or Kruskal-Wallis test. A p-value of <0.05 was expressed statistically significant. ResultsThe Cell viability was substantially reduced in the nicotine group compared to the untreated group, accompanied by changes in cell morphology. In contrast, Nrf2 expression increased significantly (p=0.010) in the 5 mM nicotine group compared with the control group. The MDA levels were not significantly distinct across groups (p=0.056). Cell migration was delayed significantly in the 5 mM nicotine group at 72 hours after scratching compared to the control group. ConclusionNicotine decreased HGFs viability and increased Nrf2 expression significantly in a dose-dependent manner. Nicotine at 5 mM concentration did not alter MDA levels but delayed cell migration.
Co-Authors Adhara Yasmine Akbar Wibriansyah Ardinansyah, Agus Atmaji, Moch Azmi, Sabrina Dwifani, Limanda Fachry Abda El Rahman Fajrin Priadinata, Akhmad Ferdila Sardan, Hasti Ferlianti, Rika Ferryal Basbeth Helwiah Umniyati Helwiyah Umniyati Indra Kusuma Kannisa Ashri Wulandari Kesumaningtias, Raden Roro Widorini Khairani Ayu Lestari Maheswari, Anindya Puspita Mart Hindrasyah Pandia, Dhayu Muhammad Arsyad Muhammad Kautsar, Muhammad Ndaru Andri Damayanti Nidya Rhenatama Norwicha Rahmasari Putri Permana, Dharma Restu Syamsul Hadi Ridhayani Hatta, Ridhayani Rifqatussaadah Tw, Afrizal Widya Pratiwi Yulia Suciati