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All Journal Sainstek Agrosains: Jurnal Penelitian Agronomi Jurnal Pertanian Agros JURNAL AGROHITA Jurnal Hilirisasi IPTEKS (JHI) JERAMI Indonesian Journal of Crop Science Jurnal Sains Agro Teaching and Learning Journal of Mandalika (Teacher) Journal of Language, Literature, and English Teaching (JULIET) Andalasian International Journal of Applied Science, Engineering, and Technology AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Jurnal Ilmiah Metansi (Manajemen dan Akuntansi) Jurnal Riset Perkebunan (JRP) Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Jagur: Jurnal Agroteknologi SAINTEK
Benni Satria
Departemen Agroteknologi Fakultas Pertanian Universitas Andalas
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Journal : Agrosains: Jurnal Penelitian Agronomi

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Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro Amalina Wahyuni; Benni Satria; Aprizal Zainal
Agrosains : Jurnal Penelitian Agronomi Vol 22, No 1 (2020): Agrosains : Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.65 KB) | DOI: 10.20961/agsjpa.v22i1.36007

Abstract

Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.
Co-Authors - Andasuryani Aca, Natasya Putri Aditya, Rezky Affitra, Muhammad Darrel Afrima Sari Al-Hamda, Habiburrahman Malik Amalina Wahyuni Annisa Najla Salsabila Apri Anwar, Aswaldi Aprisal Aprisal, Aprisal Aprizal Zainal Ardi Ardi Ardi Ardi Aries Kusumawati Armansyah Armansyah Arnelio, Rangga Auzar Syarif Candra, Muhammad Cherlinda, Dwi Damayanti, Elok Daulay, Azri Gilang Ramadhan Denal Putra Melketa Denal Putra Melketa Dewi Nopita Dongoran, Adelina Syakinawaty Ekasari Putri, Nurwanita Elsa Ernawati Nainggolan Emil Salim Erona, Meisilva Etti Swasti Ferry Lismanto Syaiful Fitri HZ, Natasya Gustian Gustian Hanifah hanifah hanifah Hendra Saputra Hendra Saputra Indra Dwipa KRISTINA, NILLA Mawati, Idel Muhammad Rafli Ibrahim Musliar Kasim Nelvia Kurnia Neswati Neswati Netti Herawati Netti Herawati Novizar Nazir Nurmahmudi, Ikhwan Obel, Obel Puan Habibah Rachmad Hersi Martinsyah Raesi, Syahyana Ramadhani, Hanggraini Ramdani Bayu Putra Ryan Budi Setiawan Satria Agust Silvia Permata S Silvia Permata Sari Siska Efendi Siska Efendi Siti Hajar Pulungan Subagiono Subagiono Syahyan Raesi Syahyan Raesi Trisia Wulantika Trizelia . Tuty Anggraini Veithzal Rivai Zainal Wahyuni, Ony Tri Warnita Yuliana, Ledi Yurniwati Yurniwati Yusniwati Yusniwati Zulfadly Syarif