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All Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions Journal of Health Sciences INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal) Jurnal Pengabdian Kesehatan Beguai Jejama
Suliati Suliati
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli Endah Prayekti; Suliati Suliati; Dwi Agustin Wulandari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1906

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
Co-Authors ., Pestariati Anita Dwi Anggraini Ayu Puspitasari Della Ika Putri Damayanti Diah Titik Mutiarawati Dwi Agustin Wulandari Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Hindaryani, Nurul Ilma Ilma Juliana Christyaningsih Kiaonarn Ongko Waluyo Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nadeak, Juan Carlos Nuning Marina Pengge Prayekti, Endah Retno Sasongkowati Salsyabillah Ramadhani HD. Widya Sasongkowati, Retno Sri Utami Suhariyadi Suhariyadi Widyanur Wisnu Istanto