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Journal : International Journal of Advances in Applied Sciences

Double step method in lipid extraction from biomass Aurantiochytrium sp powder Endah Sulistiawati; Suhendra Suhendra; Martomo Setyawan; Anis Herliyati Mahsunah
International Journal of Advances in Applied Sciences Vol 12, No 4: December 2023
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v12.i4.pp376-383

Abstract

Aurantiochytrium sp is a marine microalgae that is rich in lipids. Extraction of lipids from microalgae requires effort to select a suitable solvent and extraction method. This research used a double-step extraction method to study a mixture of n-hexane and methanol as a solvent. The variables studied were stirring time, the n-hexane to methanol (H/M) mixture ratio, and the solvent-to-biomass ratio (S/B). This research concluded that an optimum stirring time was 30 min, and a mixture of n-hexane and methanol solvents with a volume ratio of 1:1 is optimum. The optimum solvent-to-biomass ratio was S/B=20 mL/g dry microalgae. Under these conditions, the yield of oil was 83.88%. Double-step extraction can increase the yield by 10-40%.
Energy efficient direct transesterification of Nannochloropsis sp. using hydrodynamic cavitation Nirmalasari, Jiran; Setyawan, Martomo; Jamilatun, Siti; Pitoyo, Joko; Hakika, Dhias Cahya
International Journal of Advances in Applied Sciences Vol 14, No 2: June 2025
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v14.i2.pp394-405

Abstract

The increasingly limited supply of fossil fuels requires renewable fuel as an alternative source. Nannochloropsis sp. is a microalgae species containing a lipid content of between 12 and 53%, which can be converted to biofuel as an alternative source of fossil fuels through a transesterification process. Up to this date, the literature has reported no studies on biodiesel production from Nannochloropsis sp. via direct transesterification with catalyst using hydrodynamic cavitation. The direct transesterification process introduced 7.5 g of microalgae, 40 ml of methanol, 90 ml of hexane, and 0.0225 g of sodium hydroxide into the sample chamber. These mixtures were passed within the cavitation using a pressure driver and transformed into fatty acid methyl ester (FAME). The catalytic hydrodynamic cavitation method produces a higher extract yield than the stirring one. Regarding the FAME composition, the catalytic hydrodynamic cavitation method is dominated by saturated fatty acid (palmitic), while the stirring catalytic method is dominated by monounsaturated fatty acid (oleic). The hydrodynamic cavitation method provides a lower average degree of unsaturation and shorter chain length than the stirring catalytic method.