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Population Dynamics of Pseudomonad fluorescent Isolate Pf-122 In Formula in Different Save Containers Wuryandari, Yenny; Wiyatiningsih, Sri; Suwandi
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0933

The development of biopesticide formulas is carried out by optimizing the use of natural media materials. The formula is made easily, cheaply, and can still maintain the viability of bacteria. This study aims to determine the effect of various formulas and storage containers on the viability of Pseudomonad fluorescent Pf-122. Eight kinds of formulas include; 1) Non-hydrolyzed rice crab at a dose of 400 (K400), 2) at a dose of 200 (K200), 3) Hydrolyzed papaya rice crab at a dose of 400 (KP400), 4) at a dose of 200 (KP200), 5) Pineapple hydrolyzed rice crab with a dose of 400 (KN400), 6). at a dose of 200 (KN200), 7) Media King'S B at a dose of 400 (KB400), 8). at a dose of 200 (KB200). The formula is stored in a plastic and paper storage container. Population dynamics were observed at 1-week intervals. In the paper container, at week 1, the highest bacterial population was in the KP 400 and K400 formulas. At weeks 3 to 8, the population experienced a very rapid decline in all formulas, except for the KN400 formula. In the KN400 formula, at weeks 2 and 3, the population reached 107 CFU / ml and experienced a slow decrease until week 8 (population 101CFU / ml). In plastic containers, at week 1, the highest bacterial population was in the formula KB200, K200, and KP400. At the 3rd to 8th week of observation, the population experienced a rapid decline in all formulas, except for formula K400. In the K400 formula, starting from weeks 2 and 3, the population reached 106 CFU / ml and decreased until the 8th week (population 103CFU / ml). As for the KN400 formula, it can still last until the 7th week with a population of 102 CFU / ml. The longest survival rate of Pf-122 bacteria in the two storage containers was the KN400 formula. The best survival time of Pf-122 bacteria (long-lasting time and high population) is the formula of rice crab with a dose of 400 (K400) in a plastic container.

Marketing System for Rumah Coklat Trenggalek Based on Website, Online Store, and Game as I.T. Ecosystem Pratama Wirya Atmaja; Sri Wiyatiningsih
Nusantara Science and Technology Proceedings International Seminar of Research Month Science and Technology in Publication, Implementation and Co
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

CV. Rumah Coklat is a new company in Trenggalek, East Java, that is in the business of production and retail selling of chocolate in cooperation with local government and cacao farmers. Because of its status as a new company and its location in Trenggalek, which is not a big city, CV. Rumah Coklat faces significant difficulties in marketing its products. To improve the situation, Technopark UPN “Veteran” of East Java is incubating CV. Rumah Coklat in order to develop its online marketing system which consists of a website, an online store, and a game application. The three elements of the marketing system are integrated and therefore are able to exchange information with other for various purposes. The game application is a freemium, which means it can be downloaded and played freely but requires the players to unlock features by buying products on the online store, and this in turn will boost CV. Rumah Coklat’s revenue as the game application’s players become potential consumers for the chocolate products. Other than that, the game application can also show advertisements for products that are being sold on the online store, give vouchers or prizes to the best players which can be redeemed on the online store, and other things that can increase brand awareness and consumer engagement. The website and the online store have been launched and the 1.0 version of the game application will be released for Android platform on Google Play Store

Socialization of Pamelo Cultivation Based on Biopesticides: a Community Service to Develop Organic Pamelo Tourism Village in Tambakmas Village, Sub-District Sukomoro, Magetan Sukomoro, Magetan Regency. Sri Wiyatiningsih; Wiwik Sri Harijani; Wahyu Santoso; Riko Setya Wijaya
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0442

Community service, concerning the development of organic Pamelo Tourism Village based on biopesticides, has been carried out in Tambakmas Village, sub-district Sukomoro, Magetan Regency. Pamelo (Citrus maxima) has potential to be developed due to its ability to grow in tropical area and its raising production. Tambakmas Village in sub-district Sukomoro, Magetan, is one of the Pamelo production areas. Stem rot, caused by Botryodiplodia theobromae which is often happened to Pamelo trees in Tambakmas village, might reduce the quality and quantity of the harvest. However, the use of chemicals to prevent stem rot must be avoided to preserve the environment. Therefore, the development of Pamelo Organic Tourism Village requires biopesticide-based organic agriculture to restrain the disease. The socialization of biopesticide-based organic pamelo cultivation has been carried out to the Pamelo farmer group in Tambakmas Village from May to July 2019, it was integrated disease control including weeding, pruning, preparing and sterilizing the soil, and application of biopesticides. The severity of stem rot disease in Pamelo trees on the observation land with integrated disease control was examined and compared to the trees on the farmers' land without integrated disease control. Results of the observation land with integrated disease control showed that the disease severity slowly decreased by 16.20%. In contrast, disease severity that happened in the land without integrated disease control increased by 5.8%. Besides, the Pamelo trees that were treated with biopesticide developed a wound healing from the stem rot disease and exhibited growth of shoots on the treated region.

The Influence of Some Types of Biological Agent Formula and the Different Application Time on the Population of the Biological Agent Bacteria and The Chili Plants Yenny Wuryandari; Sri Wiyatiningsih Suwandi
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0446

One alternative for suppressing disease and promoting plant growth is to use the formula for biological agents. The research aims to determine the best formulation of Pseudomonad fluorescent Pf-122 and to determine the right application time in stimulating the growth of chili plants. The formula is made by mixing bacterial active ingredients with semi-natural media of rice crab by hydrolyzing using pineapple and papaya, as well as organic fertilizer. The study used 2 treatment factors. The first factor is the type of formula consisting of control using only Pf-122 (F0), Pf-122 + K (rice crab) (F1), Pf-122 + KN (rice crab by hydrolyzing using pineapple) (F2), Pf-122 + KP (rice crab by hydrolyzing using papaya) (F3). The second factor is application time; watering five days before planting and watering while planting. The results showed that plant growth, namely plant height, number of leaves, root length, and root weight did not differ statistically significantly from all treatment types of application. When viewed from the time of application of the formula, there is also no difference in the growth of chili plants. However, when viewed from the average value, the control without formula tends to show a lower growth value compared to that given the formula. In the generative growth phase, which is when the flowers appear, the chili plants are given a formula faster than without the formula. Application for all types of formulas, the time of occurrence of flowers is not significantly different. The number of Pf-122 populations in the rhizosphere decreases and increases are unstable in all formula applications. The high Pf-122 population in the rhizosphere does not show high chili growth either

Utilization of Fobio and Streptomyces sp. in Improving the Growth of Shallots Sri Wiyatiningsih; Penta Suryaminarsih; Gusdino Hasyidan
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1507

Shallots (Allium ascalonicum L.) is a spice vegetable commodity that is needed by the community as a cooking spice to increase the taste of eating pleasure and for traditional medicines that are efficacious for health. Control using chemicals is believed to be effective in overcoming pathogen attacks, but the continuous addition of chemical pesticides will have a negative impact on the environment and consumers. To deal with the negative impact of the use of these chemicals, namely by applying a sustainable farming system that was originally a conventional farming system and includes organic agriculture (Fobio biopesticide application) and biological (Application of Streptomyces sp. biological agents). The purpose of this study was to determine the effectiveness of the formulation of the biopesticide Fobio and the biological agent Streptomyces sp. to increase the growth of leeks and minimize the negative impact of using fungicides in Bulugunung Village, Plaosan District, Magetan Regency but still have the potential for maximum harvest success. The study was conducted from March to May 2021. This study used a Completely Randomized Design (CRD) consisting of 4 treatments with 6 replications. Each experimental unit consisted of 100 plants. Observation parameters consisted of leaf height and number of leaves of shallot plants. The results showed that the application of biopesticide Fobio and biological agents Streptomyces sp. did not significantly affect the height of the shallot plant and had a significant effect on the number of leaves of the shallot plant

Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.

The Utilisation of Botanical Pesticide as Pest Control in The Management of Organic Vegetable Farming Faradila Amir; Sri Wiyatiningsih
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3115

Botanical pesticides are eco-friendly pesticides derived from plant extracts. Botanical pesticides can be used as pest control in organic vegetable farming. Some pests are known to attack organic vegetables, such as the large cabbage-heart caterpillar (Crocidolomia pavonana) and aphids (Myzus persicae) attack the cauliflower plant; grasshopper (Atractomorpha similis) and flea beetle (Phyllotreta nigripes) attack the green mustard plant, and the beetle (Epilachna borealis) attacks tomato plants. Some botanical pesticides used to control these pests are derived from soursop leaf extract, paitan leaf extract, and neem fruit extract. The results of the application of these pesticides can reduce the pest population; the percentage of pest population decline in C. pavonana is 30%; M. persicae s by 65.5%; A. similis by 66.6%; Ph. nigripes by 60%; and E. borealis as much as 66.6%.

Diversity of Important Pests and Natural Enemies in Rice Plants Nauval Zakqy; Sri Wiyatiningsih
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3117

The main obstacle that is often faced by farmers is the presence of Plant Pest Organisms (OPT), one of the pests is the types of pests on rice plants. Diversity of pests and natural enemies of rice is an activity of collecting and compiling data and information regarding the presence of pests and natural enemies in rice cultivation. The purpose of the study was to obtain data on the types of pests and natural enemies in lowland rice cultivation. The results showed important pests on rice plants, namely green leafhoppers (Nephotettix virescen), walang sangit (Leptocorisa oratorius), brown leafhoppers (Nilaparvata lugens), armyworms (Mythimna separate), grasshoppers (Oxysa spp), ganjur (Orseolia oryzea), birds finches (Lonchura sp) and mouse (Rattus argentiventer). As for natural enemies, namely spiders (Lycosidae), tomcat (Paederinae), dragonflies (Orthetrum Sabina), wasps (Vespula germanica) and beetle koksi (Coccinella septempunctata). The presence of natural enemies in rice cultivation can affect the level of pest populations in rice cultivation.

Utilization of Fobio and Streptomyces sp. in Improving the Growth of Shallots Sri Wiyatiningsih; Penta Suryaminarsih; Gusdino Hasyidan
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1507

Shallots (Allium ascalonicum L.) is a spice vegetable commodity that is needed by the community as a cooking spice to increase the taste of eating pleasure and for traditional medicines that are efficacious for health. Control using chemicals is believed to be effective in overcoming pathogen attacks, but the continuous addition of chemical pesticides will have a negative impact on the environment and consumers. To deal with the negative impact of the use of these chemicals, namely by applying a sustainable farming system that was originally a conventional farming system and includes organic agriculture (Fobio biopesticide application) and biological (Application of Streptomyces sp. biological agents). The purpose of this study was to determine the effectiveness of the formulation of the biopesticide Fobio and the biological agent Streptomyces sp. to increase the growth of leeks and minimize the negative impact of using fungicides in Bulugunung Village, Plaosan District, Magetan Regency but still have the potential for maximum harvest success. The study was conducted from March to May 2021. This study used a Completely Randomized Design (CRD) consisting of 4 treatments with 6 replications. Each experimental unit consisted of 100 plants. Observation parameters consisted of leaf height and number of leaves of shallot plants. The results showed that the application of biopesticide Fobio and biological agents Streptomyces sp. did not significantly affect the height of the shallot plant and had a significant effect on the number of leaves of the shallot plant

Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.

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