Article Per Year (5 Year)
p-Index From 2020 - 2025
2.062
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter The Journal of Experimental Life Sciences (JELS) Biotropika Natural B The Journal of Pure and Applied Chemistry Research Molecular and Cellular Biomedical Sciences (MCBS)
Aris Soewondo
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Brawijaya University, Malang, 65145, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary Other

Published : 35 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 35 Documents
Search

 1   2   3   4 
Expression of Erythroid Progenitor Cells and Erythrocytes on Dexamethasone Induced-Mice Wira Eka Putra; Muhaimin Rifa'i; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 3, No 1 (2015)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dexamethasone (Dex) is synthetic corticosteroid, known as anti-inflamation drug to ameliorate autoimmune diseases. It worked by inhibiting production of proinflamatory citokines. The aim of this experiment was to confirm the effect of administration of Dex on erythroid progenitor cells, TER-119+VLA-4+ and erythrocytes, TER-119+VLA-4- expression from bone marrow compartments. Two weeks old BALB/c mice were used and grouped into 3 injection treatments of Dex with six replications i.e. no injection (control), 0.5 mg/kg BW (dose 1), 10 mg/kg BW (dose 2). To investigate the therapeutic effect of dexamethasone, the mice were sacrified on day-7. The bone marrow cells were isolated and analyzed by flow cytometry. Data analysis was confirmed with the Kruskal-Wallis test followed by Mann-Whitney test with significance level (α) of 0.05. The result showed that administration of Dex on BALB/c mice increase the expression of erythroid progenitor cells, TER-119+ VLA-4+ and erythrocytes, TER-119+VLA-4- from bone marrow.Key words: Dex, erythrocytes, erythroid progenitor cells
Histopatologi Hati dan Paru Mencit (Mus musculus) yang Terpapar Formalin dan Benzo(α)pyrene Rike Wahyuningroom; Aris Soewondo; Sri Widyarti
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian formalin dan benzo(α)pyrene terhadap struktur jaringan hati dan paru mencit (Mus musculus). Mencit jantan berumur dua bulan terpapar formalin dosis 2 mg/kg berat badan dengan perlakuan oral selama 60 hari dan terpapar benzo(α)pyrene dosis 250 mg/kg berat badan dengan perlakuan injeksi intraperitoneal sebanyak empat kali setelah 30 hari dengan selang waktu satu hari. Stuktur histologi hati dan paru mencit dianalisis secara deskriptif. Jumlah hepatosit yang mengalami nekrosis dianalisis dengan menggunakan uji Mann-Whitney pada software SPSS 16.0 for Windows. Hasil dari penelitian ini menunjukkan bahwa pemberian formalin, benzo(α)pyrene dan kombinasi antara formalin dan benzo(α)pyrene meningkatkan jumlah hepatosit yang mengalami nekrosis. Nekrosis terjadi di sekitar area portal triad dan di sekitar area vena sentralis pada hati mencit, sedangkan nekrosis terjadi di sekitar area vena pada paru mencit. Kata kunci: benzo(α)pyrene, formalin, hati, nekrosis, paru
Amplifikasi Gen COI dan 16s rRNA dari Invertebrata Laut Plakobranchus ocellatus Fitria Eka Aprilia; Aris Soewondo; Nashi Widodo
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies secara molekuler tidak terlepas dari isolasi DNA dan amplifikasi gen COI dan 16s rRNA. Keberhasilan isolasi DNA dan amplifikasi gen COI dan 16S rRNA merupakan langkah awal yang penting untuk studi mengenai hubungan kekerabatan, evolusi dan identifikasi spesies. Penilitian ini bertujuan untuk mengamplifikasi gen COI dan 16S rRNA dari invertebrata laut yang diambil dari Raja Ampat, Plakobranchus ocellatus. Sepasang primer COI universal LCO-1490 dan HCO-2198 digunakan untuk mengamplifikasi fragmen gen COI dari P.ocellatus, sedangkan amplifikasi gen 16s rRNA menggunakan sepasang primer 16Sar-L dan 16Sbr-L. Fragmen gen COI dan 16S rRNA berhasil teramplifikasi dengan panjang amplikon masing-masing sekitar 680 bp dan 450 bp.   Kata kunci: COI, gen, identifikasi, Plakobranchus ocellatus dan 16S rRNA ABSTRACT The first important step to study about organism relationship, evolution and species identification are DNA isolation and amplification of 16S rRNA and COI genes. This research aimed to amplify COI and 16S rRNA genes of marine invertebrates taken from Raja Ampat, Plakobranchus ocellatus. A pair of COI universal primer LCO-1490 and HCO-2198 was used for ampification a fragment COI gene P.ocellatus, while a fragment of the 16s rRNA gene was amplified using 16Sbr-L and 16Sar-L primer. Either  a fragment COI or 16S rRNA genes was successfully amplified, with a length amplicon about 680 bp and 450 bp, respectively. Keywords : COI, gene, identification, Plakobrancus ocellatus, and 16S rRNA
KADAR MDA SPERMATOZOA SETELAH PROSES PEMBEKUAN Khairatul Insani Febrianti; Sri Rahayu; Agung Pramana W.M; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 2, No 3 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan penelitian ini adalah untuk mengetahui efek lama pembekuan semen terhadap kadar MDA spermatozoa dan persentase viabilitas spermatozoa. Sampel yang digunakan berupa  semen beku sapi Limousin dengan lama pembekuan 2 bulan dan 3 bulan yang dikoleksi dari BBIB Singosari. Semen beku yang telah dithawing diencerkan dengan Phosphate Buffer Saline (PBS) kemudian disentrifugasi untuk memisahkan spermatozoa dan seminal plasma. Kadar MDA spermatozoa diukur dengan menggunakan uji TBA dan spektrofotometer. Pengamatan viabilitas spermatozoa dilakukan dengan menggunakan pewarnaan eosin-negrosin. Data berupa kadar MDA spermatozoa dan persentase viabilitas dianalisis dengan uji-t berpasangan dan korelasi pearson menggunakan spss 16.0. Hasil analisis menunjukkan bahwa kadar MDA spermatozoa pada lama pembekuan 2 bulan (0,0777±0,008 nM) dan 3 bulan (0,0919±0,016 nM) tidak berbeda nyata (p<0,05). Terdapat korelasi negatif antara persentase viabilitas dan kadar MDA pada spermatozoa. Kata kunci: MDA, semen beku, spermatozoa 
Peran Alpha-S2 kasein Susu Kambing PE Terhadap Perbaikan Morfologi dan Histologi Membran Synovial Tikus Rheumatoid Arthritis Rivqi Rifa Bia; Fatchiyah Fatchiyah; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 2, No 5 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk menganalisa efek pemberian alpha-S2 kasein  susu kambing PE terhadap perubahan morfologi dan histologi membran sinovial tikus Rheumatoid Arthritis (RA). Tahapan penelitian meliputi pembuatan RA menggunakan multidosis CFA (Complete Freud Adjuvant. Kelompok perlakuan dibagi menjadi (C: Kontrol; CM: Kontrol + alpha-S2 kasein; RA: Rheumatois Arthritis; RAM: Rheumatois Arthritis + alpha-S2 kasein). Treatment RA dilakukan  selama 2 bulan dengan dosis 2mg/kg. Analisa morfologi dilakukan dengan skoring perubahan bentuk kaki dan histologi menggunakan metode HE yang divisualisasi menggunakan mikroskop BX53. Hasil penelitian menunjukkan adanya perbaikan morfologi yang ditandai dengan penurunan hasil skor klinis (C = 0; RA = 4; CM = 0; RAM = 1.3). Perbaikan histologi terletak pada penurunan jumlah hiperplasia pada kelompok RA + alpha-S2 kasein menuju kondisi normal dimana penurunan level tersebut mengidikasikan alpha-S2 kasein dapat digunakan sebagai alternatif penyembuhan RA.
Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method Sofy Permana; Sri Widyarti; Serafinah Indriyani; Dian Siswanto; Aris Soewondo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.475 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.14

Abstract

Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting.  Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before.  The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.
Effect of Dexamethasone Administration toward Hematopoietic Stem Cells and Blood Progenitor Cells Expression on BALB/c Mice Wira Eka Putra; Aris Soewondo; Muhaimin Rifa&#039;i
The Journal of Pure and Applied Chemistry Research Vol 4, No 3 (2015)
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.457 KB) | DOI: 10.21776/ub.jpacr.2015.004.03.221

Abstract

Hematopoiesis play important roles on maintaining body homeostasis. Dexamethasone (Dex) known as synthetic glucocorticoids and widely affect many regulations i.e. immune system, inflammation, reproduction and all aspects of metabolism. Generally Dex were used as therapeutic agentsto ameliorate many kind of diseases including cancer and auto-immune diseases. The aims of this research were to investigate the effect of Dex administration toward expression of hematopoietic stem cells and blood progenitor cells. Two weeks old BALB/c mice were used and treated with 3 groups of Dex administration i.e. untreated-mice, 0.5 mg/kg BW, and 10 mg/kg BW. Mice were sacrificed on day-7 after administration of Dex. The bone marrow stromal cells were isolated, then followed by flow cytometry analysis. The result showed that, Dex administration with dose 0.5 mg/kg BW significantly increase the expression of hematopoietic stem cells, CD34+, chemokines, SDF-1+, erythroid progenitor cells, Gr-1-TER-119+, granulocytes, Gr-1+, and B progenitor cells, B220+.
Peran Penting Sel T Regulator CD8+CD25+FOXP3+ Menjaga Homeostasis Sel T pada Transplantasi Alogenik (ESSENTIAL ROLES OF CD8+CD25+FOXP3+ REGULATORY T CELLS IN THE MAINTENANCE OF T CELLS HOMEOSTASIS IN ALLOGENEIC TRANSPLANTATION) Muhaimin Rifa’i; Aris Soewondo
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.476 KB)

Abstract

Tolerance between donor and recipient is a key for successful allogeneic transplantation. To determinethe involvement of CD8+ T cells in regulating T cell homeostasis in allogeneic transplantation, spleen cellswere analyzed post bone marrow transplantation. In this study, C57BL/6 mice were used as donor andBALB/c mice were used as recipients. BALB/c mice were irradiated with a lethal dose of 850 rad prior tothe transplantation. Twenty-four hours post a radiation BABL/c mice were transplanted with bone marrowcells (10x106) derived from C57BL/6 mice that free from mature T cells. Analysis of hematopoietic cells inthe spleen was done two months post-transplantation. The results showed that CD8+CD25+FOXP3+ T cellin collaborasion with CD4+ regulatory T cell contribute to the prevention of graft-versus-host diseases(GVHD). In this study at was demonstrated that naïve CD4+CD62L+ T cells dominated the sub set of Tcells, whereas the development of CD4+CD44+ memory T cells are very limited. In conclusion, that regulatoryT cells maintain normal homeostasis by maintaining T cells remained at naïve status.
ANALISIS KADAR LUTEINIZING HORMONE (LH) PADA SERUM SAPI FRIESIAN HOLSTEIN POST PARTUM DENGAN PENAMBAHAN SELENIUM DAN VITAMIN E Ririn Dwi Prasetiani; Sri Rahayu; Aris Soewondo
Biotropika: Journal of Tropical Biology Vol 3, No 3 (2015)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ANALISIS KADAR LUTEINIZING HORMONE (LH) PADA SERUM SAPI FRIESIAN HOLSTEIN POST PARTUM DENGAN PENAMBAHAN SELENIUM DAN VITAMIN E   Ririn Dwi Prasetiani1), Sri Rahayu2), Aris Soewondo3) 1),2),3) Laboratorium Biomolekuler dan Seluler, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya, Malang 65145, Jawa Timur, Indonesia. E-mail: ririndwi68@yahoo.com ABSTRAK Sapi Friesian Holstein (FH) merupakan bangsa sapi yang memiliki produksi susu tertinggi dibandingkan bangsa-bangsa sapi perah lainnya. Banyak peternak yang menginginkan peningkatan produktifitas sapinya dengan asumsi setiap 1 ekor sapi setidaknya melahirkan 1 ekor anak sapi setiap tahun. Namun banyak masalah yang ditimbulkan pada sapi postpartum, salah satunya akibat peningkatan radikal bebas pada tubuh sapi postpartum, sehingga asumsi tersebut tidak terpenuhi. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian selenium dan vitamin E secara intramuskular terhadap kadar Luteinizing Hormone (LH) serum sapi FH postpartum. Hewan coba yang digunakan adalah 6 ekor sapi bunting 7 bulan yang dibagi menjadi 2 kelompok, masing-masing 3 ekor untuk kelompok kontrol (P0/ tidak diinjeksi selenium dan vitamin E) dan perlakuan (P1/di injeksi sodium selenite 1,5 mg/ml + vitamin E 50 mg/ml). Injeksi dilakukan 5 kali yaitu saat sapi bunting 7 bulan, 8 bulan, saat 2 minggu sebelum melahirkan, 7 hari dan 14 hari setelah melahirkan. Pengambilan serum untuk pengujian kadar LH dilakukan dua kali dari masing-masing hewan coba, yaitu pada 25 hari dan 45 hari postpartum. Pemeriksaan kadar LH dilakukan dengan teknik ELISA (Enzyme-Linked Immunosorbent Assay). Hasil penelitian menunjukkan bahwa pemberian sodium selenite 1,5 mg/ml + vitamin E 50 mg/ml cenderung menyebabkan peningkatan LH serum sapi FH pada 25 dan 45 hari postpartum.   Kata kunci: LH, sapi FH, selenium, vitamin E ABSTRACT Holstein Friesian dairy cows (FH) is a group of cows that have the highest milk production compared to other dairy cattle groups. Many farmers want to increase the productivity of the cow with the assumption that every one cow give birth to at least 1 cow each year. But many problems caused in postpartum cows, one of which is due to the increase of free radicals in the postpartum cows body, so the assumptions are not met. This study was conducted to analyze the effect of selenium and vitamin E intramuscularly in LH levels serum of dairy cow FH postpartum. There are 6 cows divided into two groups, 3 cows for each group. First group is control (P0) which no administration of sodium selenite and vitamin E, and second group is treatment (P1) that intramuscularly injected of sodium selenite 1.5 mg/ml + vitamin E 50 mg/ml). Injection of selenium and vitamin E conducted 5 times when 7 months, 8 months cows gestation, two weeks before calving, seven days after calving and 14 days after calving. Serum sampling for testing levels of LH take in 25 days and 45 days postpartum on each cows. The level of LH was conducted by ELISA (Enzyme-Linked Immunosorbent Assay). The results showed that administration of sodium selenite 1.5 mg/ml + vitamin E 50 mg/ml causes increased LH levels serum that taken 25 days, 45 days postpartum.   Keywords: dairy cow FH, LH, selenium, vitamin E
Pengaruh Ekstrak Ethanol Kemangi (Ocimum canum Sims.) terhadap Struktur Histologi Testis Mencit (Mus musculus) Jantan Ivakhul Anzila; Agung Pramana W. M.; Aris Soewondo; Sri Rahayu
Biotropika: Journal of Tropical Biology Vol 5, No 1 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.01.4

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak ethanol kemangi (Ocimum canum Sims.) terhadap struktur histologi testis mencit jantan (Mus musculus). Penelitian ini menggunakan 9 ekor hewan coba mencit strain Balb/c jantan berumur 3 bulan, berat badan 20 - 30 g. Hewan coba dibagi menjadi tiga (3) kelompok kontrol tanpa pemberian ekstrak kemangi (P0), kelompok yang diberi ekstrak kemangi dengan dosis 100 mg/kgBB (P1) dan 200 mg/kgBB (P2). Ekstrak kemangi diberikan setiap hari secara oral selama 35 hari. Parameter yang diamati adalah diameter tubulus seminiferus, tebal epitel germinal, jumlah lapisan germinal dan jumlah sel spermatogenik meliputi spermatogonia, spermatosit dan spermatid. Data yang diperoleh dianalisis dengan One-way ANOVA (p ≤ 0,05). Jika menunjukkan perbedaan yang bermakna, maka dilanjutkan dengan uji Least Significant Difference (LSD). Hasil penelitian menunjukkan bahwa pemberian ekstrak kemangi dosis 200 mg/kgBB meningkatkan diameter tubulus seminiferus, tebal epitel germinal, dan jumlah sel spermatid
Co-Authors Agung Pramana W. M. Agung Pramana W.M Agung Pramana W.M Agung Pramana W.M Agung Pramana Warih Marhendra Agung Pramana Warih Marhendra Ahmad Shobrun Jamil Al Faizah, Belinda Nabiila Anaqoh Roudhotul Jannah Winarso Anzila, Ivakhul Ardiansyah, Esha Asyhari, Firda Nuri Atho'illah, Mochammad Fitri Dian Siswanto Dliyauddin, Moh Fadlilah, Dawama Nur Farida Rachmawati, Farida fatchiyah . Fatchiyah Fatchiyah Fatchiyah Fatchiyah Fikriya Novita Sari Fikriya Novita Sari Firstiantono, Andista Fitria Eka Aprilia Iskandar, Rossdannisa Isna Hapsari, Ajeng Hanum Ivakhul Anzila Izati, Rahmi Kavitarna, Septhyanti Aprilia Khairatul Insani Febrianti Khairatul Insani Febrianti Laili Nur Zaidah Mansur Ibrahim Moch Sasmito Djati Muhaimin Rifa&#039;i Muhaimin Rifa&#039;i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa’i Muhaimin Rifa’i Muhammad Halim Natsir Muhammad Halim Natsir Nashi Widodo Nurul Shafa Paramita Putri, Nenis Try Melani Rifa'i, Muhaimin Rifa’i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Dwi Prasetiani Ririn Dwi Prasetiani, Ririn Dwi Rivqi Rifa Bia Rizky Senna Samoedra Rizky Senna Samoedra Robitoh Desi Kurniasari Sa'adah, Nur Alfi Maghfirotus Sakti, Muhammad Wisam Wira Sakti, Sefihara Paramitha Sari, Fikriya Novita Serafinah Indriyani Setyaki Kevin Pratama Setyaki Kevin Pratama Shinta Dwi Riestanti Shinta Dwi Riestanti, Shinta Dwi Shinta Oktya Wardhani, Shinta Oktya Siti Nur Arifah, Siti Nur Sofy Permana Sri Rahayu Sri Rahayu Sri Rahayu SRI RAHAYU Sri Widyarti Triandari, Ade W. M., Agung Pramana Warih Marhendra, Agung Pramana Wira Eka Putra Wisnu Barlianto YOGA DWI JATMIKO Yuyun Indriani Kartiko Santi Zulfatim, Heni Sukma