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All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Makara Journal of Science Indonesian Journal of Jamu
Sri Widyarti
Biology Department, Faculty Of Mathematics And Natural Sciences Brawijaya University, Malang Indonesia
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Journal : Biotropika

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Antibacterial Activity of Pluchea indica Leaf Extract was Increased After Being Fermented with Saccharomyces cerevisiae and Added with its Cell-Free Supernatant Rismawati, Ria; Jatmiko, Yoga Dwi; Widyarti, Sri
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.04

Abstract

Multi Drug Resistance (MDR) is a global health problem that endangers public health and can lead to death. Therefore, novel antibacterial agents are required derived from medicinal plants, one of them is beluntas (Pluchea indica) which has high potential as an antibacterial. Fermentation or addition of cell-free supernatant of Saccharomyces cerevisiae is thought to increase the antibacterial content of an herb. This study aimed to evaluate the effect of fermentation and the addition of Cell-Free Supernatant (CFS) of S. cerevisiae to enhance antibacterial activity of P. indica leaf extract. Fresh and dried leaves of beluntas were used in this study. The dried leaves in the form of powder was boiled at 100oC for 45 minutes. Fresh leaves were homogenized by blending. The extract of P. indica was used for fermentation and addition of CFS of S. cerevisiae. CFS as much as 60 mL dan fermentation with cell density of 7.53x105 CFU/mL with 100 mL of P. indica leaf extract were centrifuged at 10,000 rpm for 20 minutes. The antibacterial test method used was the Kirby-Bauer method against Escherichia coli and Staphylococcus aureus. The results showed an increase in antibacterial activity as indicated by the increasing diameter of the inhibition zone either by fermentation or addition of CFS with inhibition zone diameter was 3.85 – 4.81 mm against E. coli and 4.63 – 5.12 mm against S. aureus. The fermented P. indica and addition of CFS was shown to be potentially developed as antibacterial agents. Keywords: Antibacterial, CFS, fermentation, Pluchea indica, Saccharomyces cerevisiae
Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W A’liyatur Rosyidah Aditya Tri Hernowo Ahmad Lubab Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Aristyani, Sherry Askandar Tjokroprawiro Attabik Mukhammad Amrillah Aulanni'am Azzamuddin, Haidar Bella Novinda Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Damayanti, Febriane Eka Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Dyah Kinasih Wuragil Eko Puji Astuti Erly Nur Aisyah Estri Laras Arumingtyas Farida Rachmawati, Farida Fatinah, Arik Arubil Hafiedz Maulana Harits Amrulloh Intan Sartika Is Karima Jannah, Nur Karima, Is Khansa, Shafanony Khoirin Maghfiroh Linda Kartika Dewi Lulut Dwi Nurmamulyosari M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai'i Muhamad Aris Widodo Naqiyah A. Mulachelah Nashi Widodo Natasya Adiba Zahrah Noer Hasanah Nunung Harijati Nur Permatasari Nur Permatasari Nuzulul Hikmah Olly Indrajani Puspitoputri, Alya H. Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rifa’i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rismawati, Ria Rizki Amalia Rochmatika, Lailiyavina Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, A’liyatur Sakti, Sefihara Paramitha Sapti Puspitarini Sari, Fikriya Novita Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Ulinnuha, Maria Wibi Riawan Widodo Widodo Yayu Tsamrotul Fuadah, Yayu Tsamrotul Yoga Dwi Jatmiko YOGA DWI JATMIKO Yuni Kilawati Zauhani Kusnul