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All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Makara Journal of Science Indonesian Journal of Jamu

Sri Widyarti

Biology Department, Faculty Of Mathematics And Natural Sciences Brawijaya University, Malang Indonesia

Author-ID : 385924

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model Hafiedz Maulana; Nuzulul Hikmah; Amandia Dewi permana Shita; Nur Permatasari; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

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Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment streptozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t-test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides.

Dose Optimization of Calcusolâ„¢ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice (Mus musculus) Ahmad Soni; Muhammad Sasmito Djati; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

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Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusolâ„¢ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusolâ„¢ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusolâ„¢ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusolâ„¢ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm.

Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Teguh Suprihatin; Sri Widyarti; Muhaimin Rifa'i; Sri Rahayu
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

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Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 Â± 0.26 Ïg/mL) and at day 28 (4.01 Â± 0.26 Ïg/mL).

Penerapan Metode K-Means untuk Evaluasi Senyawa Anti Kanker Berdasarkan Kajian Proteomik Sel MCF7 Mochammad Pratama Viadi; Marji Marji; Sri Widyarti
Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Vol 3 No 5 (2019): Mei 2019
Publisher : Fakultas Ilmu Komputer (FILKOM), Universitas Brawijaya

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Cancer is a disease that originates from several body cells that can divide without stopping and can spread to surrounding tissues. Then split cell will attack and damage the surrounding tissue. Especially in breast cancer or occupying the top level in cancer. This cancer originates from a glandular tract in the breast which declared a malignant tumor. In the detection this disease can use bioinformatics techniques, namely proteomic studies. Output produced is a grouping of the results of the anti-cancer process on the development of cancer in the form of clustering using the K-Means process which is divided into 2, active and inactive. The results of system testing were carried out using Silhoutte Coefficient and Precision Recall Accuracy. In the Silhoutte Coefficient test getting the optimal cluster is 5 with a value of 0.6827. Whereas in testing the Precision Recall Accuracy, the Precision value was 67.59%, the Recall value was 79.57%, and the accuracy rate was at 57.71%. Using 10-fold cross validation get an average accuracy of 62.26%.

The effect of Phyllanthus niruri and Catharanthus roseus on Macrophage Polarization in Breast Cancer Mice Model: The Effect of P. niruri and C. roseus in Breast Cancer Mice Model Sakti, Sefihara Paramitha; Sari, Fikriya Novita; Rachmawati, Farida; Widyarti, Sri; Rahayu, Sri; Soewondo, Aris; Jatmiko, Yoga Dwi; Rifa'i, Muhaimin
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.03

Cancer death cases have increased yearly, and there are estimated to be 21.6 million cancer cases in 2030. Studies of herbal compounds for cancer treatment alternatives are essential because cancer treatment is relatively expensive and has adverse effects. Phyllanthus niruri (Pn) and Catharanthus roseus (Cr) are plants that are known as herbal medicines. Combining the two plants is expected to prevent and enhance the immune system in breast cancer cases. This study aims to analyze the anti-cancer and immunomodulatory effects of P. niruri and C. roseus extract (PCE) in modulating macrophage polarization in breast cancer mice. Experimental animals are divided into six groups and there is healthy control (normal mice), cancer (DMBA-induced mice), cancer mice with cisplatin administration, cancer mice with PCE administration with three different doses, including dose 1 (500 mg/kg Pn + 15 mg/kg Cr), dose 2 (1000 mg/kg Pn + 75 mg/kg Cr), and dose 3 (2000 mg/kg Pn + 375 mg/kg Cr). The mice were injected with DMBA once a week for six weeks to induce cancer in mice. The breast cancer mice model was administered with PCE orally for 14 days. The expression of CD11b+IL-10+ and CD11b+IFN-γ+ demonstrated macrophage polarization. The results showed that breast cancer induction using DMBA increased the level of IL-10 and decreased the level of IFN-γ significantly compared to the normal group (p < 0.05). In specific doses, administration of PCE could reduce IL-10 levels and increase the level of IFN-γ significantly (p < 0.05). PCE can modulate the polarization of macrophages by suppressing the M2-like macrophage and increasing the M1-like macrophage. The ability of PCE to modulate macrophage polarization indicates that the combination of P. niruri and C. roseus has activity as an anti-cancer.

Potential of Kesambi Active Compound (Schleichera oleosa) as Antagonist G-Protein Estrogen Receptor 1 (GPER1) by In Silico Radita Intan Aisyah Pratiwi; Widyarti, Sri; Sumitro, Sutiman Bambang
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.07

Tamoxifen is a treatment for breast cancer patients which can cause side effects of endometrial cancer because it acts as a GPER1 agonist. Active compounds from Schleichera oleosa are known to have anticancer potential, such as schleicheol and schleicherastatin, especially their ability to prevent cell proliferation. This research conducted an in silico study to determine the potential of the active compound from S. Oleosa as a GPER1 inhibitor. In silico studies include molecular docking and molecular dynamics. The data obtained are binding affinity values, potential energy, RMSD, RMSF, and conformational changes. Active compound candidates with the lowest binding affinity were selected, namely Schleicheol 1 (SCL1), Lupeol (LU), Lupeol acetate (LA), Betulinic acid (BA), and Schleicherastatin 3 (SCR3) with an order of score -8.6, - 8.5, -8.4, -8.4 and -8.4 kcal.mol-1. When complexed with GPER1-Estradiol and GPER1-Tamoxifen, the lowest binding affinity was LU (-8.6 and -8.7 kcal.mol-1). LU binds to the same amino acid as Estradiol and Tamoxifen, namely Leu:271. Based on molecular dynamics, RMSD All (receptor complex) ranged from 3,723 to 5,098 Å, above the normal limit of 3 Å. However, RMSD All shows stability starting from 1.5 ns so that the resulting data can be used. The RMSF value showed higher fluctuations than Tamoxifen at the same binding site as Tamoxifen, including SCL1-T, LU-T, LA-T, and BA-T, which can interfere with the function of the GPER1 receptor. LU, LA, BA, SCL1-T LU-T, and LA-T with GPER1 produce the same structural changes as G15 as GPER1 antagonists. The active compound, especially lupeol, which has the lowest binding affinity, is predicted to have the potential to inhibit GPER1 in silico so that it can be proposed for further testing. Keywords: Endometrial Cancer, GPER1, Schleichera oleosa, Tamoxifen.

Influence of Marsilea crenata and Alpinia purpurata Ethanol Extract on MDA and SOD Testicular Cells of Hyperglycemia Mice Damayanti, Febriane Eka; Rahayu, Sri; Widyarti, Sri
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.04

Hyperglycemia can induce testicular damage that leads to male infertility. Herbal plants, such as Marsilea crenata and Alpinia purpurata, are used for antioxidant defense systems to repair reproductive disorders due to hyperglycemia. This study aims to evaluate the effect of M. crenata and A. purpurata ethanol extracts on MDA and SOD testes of hyperglycemia mice. This study used a completely randomized design with seven treatment groups (n=4), namely N (control group), H (hyperglycemia mice), Met (hyperglycemia mice given metformin), D1 (0. 09 mg.g-1 M. crenata), D2 (0.2 mg.g-1 A. purpurata), D3 (0.09 mg.g-1 M. crenata + 0.2 mg.g-1 A. purpurata), and D4 (0.09 mg.g-1 M. crenata + 0.4 mg.g-1 A. purpurata). The extracts were administered orally for 17 days. Analysis of testicular MDA and SOD levels was performed by flow cytometry. Data analysis was performed with a one-way ANOVA test and continued with the Tukey test. The results showed that the D4 treatment group, compared to D3, D2, D1, Met, H, and N, showed a better decrease in MDA levels (4.47%) and an increase in SOD levels in the D4 group (4.77%). The research concludes that the combination of 0.09 mg.g-1 M. crenata and 0.4 mg.g-1 A. purpurata was an optimal dose to decrease MDA levels and increase SOD levels in the testes of hyperglycemic mice. Keywords: Alpinia purpurata, Marsilea crenata, MDA, SOD, testes.

Analysis Activity of Elephantopus scaber Leaves Extract Against Quantitative Changes of Lymphocytes Cells in BALB/c Mice After Induction of DMBA and Estrogen Rochmatika, Lailiyavina; Widyarti, Sri; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

The carcinogenic compounds such as DMBA known for having the ability to promote the DNA damage that affects the growth regulator genes. Therefore, uncontrolled cell growth will occur and change the immune system. Elephantopus scaber extract is considered as an alternative way of handling breast cancer since it capable to affect the work of the immune system. Hence, this study aims to determine the effect of E. scaber extract on quantitative changes of CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFNγ+ and CD4+TNF-α+ after induction of DMBA and estrogen. In vivo experiments were carried out by using 5-6 weeks old of female mice BALB/c strain. The extract of E. scaber leaf powder was obtained from Material Medica Batu, Malang. The experimental animals were divided into five groups such as negative control group (K-), positive control group (K+), mice treated with E. scaber extract for a week (group 1), mice treated with E. scaber extract for 2 weeks (group 2), and mice treated with E. scaber extract for 3 weeks (group 3). Induction of DMBA and estrogen was conducted by injection of DMBA (7.12 dimetylbenz (α) anthracene) 0.56 mg.kgBW-1 and estradiol hormone 0.0504 mg.kgBW-1. Mice were treated with E. scaber extract with a dose of 50 mg.kgBW-1. Lymphocytes were isolated from spleen and observed the CD4+, CD8+ T cells and proinflammatory cytokines CD4+IFN-γ+ and CD4+TNFα+ T cells through flow cytometry analysis. One-way ANOVA (p <0.05) and SPSS were used to analyze this data. The results showed that the induction of DMBA and estradiol hormone affected mice fur loss. It also decreased the immune system by lowering the number of CD4 and CD8 T cells. However, E. scaber extracts increased the number of pro-inflammatory cytokines TNF-α. Furthermore, the DMBA and estradiol induction also promoted the number of CD4+IFN-γ+ in the first week, but decreased the number of CD4+IFN-γ+ at weeks 2 and 3, then increase the number of CD4+IFN-γ+. So, the treatment of E. scaber extracts demonstrated the ability to restore the homeostasis of the immune system in the first and second weeks. Keywords: CD4+, CD8+, DMBA, estrogen, E.scaber, IFN-γ+, TNF-α+

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.03

Employing SecA Recombinant Protein to Generate Polyclonal Antibodies for the Rapid Identification of Phytoplasma Fatinah, Arik Arubil; Rifa'i, Muhaimin; Arumingtyas, Estri Laras; Widyarti, Sri
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Phytoplasma, a plant pathogen that threatens plant health, can induce diseases such as yellow wilt, virescence, phyllody, and witches’ broom. Although early detection methods using polyclonal and monoclonal antibodies have been developed, synthetic peptides offer advantages such as higher titers, predictable antigenic characteristics and superior sensitivity and specificity. Epitope mapping plays a key role in designing peptide antigens and includes gene synthesis, transformation, expression assessment, large-scale production, and purification. A study using New Zealand male White rabbits was conducted to generate antibodies against phytoplasma. The SecA (395–470) sequence, obtained from the preprotein translocase meets the criteria of being non-homologous, nonallergic, and antigenic. The three-dimensional structure of SecA (395–470) has a sequential similarity of 77–97% with certain phytoplasma types, and the protein purity exceeds 90%. The production of polyclonal antibodies was successful, achieving titers greater than 1:512,000. The SWISS model was used to predict the 3D structure of SecA in phytoplasma species, revealing structural homology with other phytoplasma species. The recombinant protein antigen SecA was able to induce high-titer antibody formation (>1:512.000). The developed lateral flow immunoassay (LFA) detects phytoplasma in plants using purified and conjugated antibodies. The peptide design, derived from Aster yellows witches-broom (SrI), effectively detects phytoplasma from various groups, especially Cactus witches’ broom phytoplasma (SrII-C) and Peanut witches’ broom phytoplasma (SrII-A). The SrI group phytoplasma was chosen as a reference sequence due to its` ability to infect plants across the broadest host range. LFIA was tested on samples from both phytoplasma-infected and healthy plants using nested PCR. The SecA sequence was successfully produced and used as an immunogen candidate against phytoplasmas.

Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W Aâ€™liyatur Rosyidah Ahmad Lubab, Ahmad Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Amrulloh, Harits Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Aristyani, Sherry Askandar Tjokroprawiro Attabik Mukhammad Amrillah Aulanni'am Azzamuddin, Haidar Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Damayanti, Febriane Eka Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Eko Puji Astuti Erly Nur Aisyah Estri Laras Arumingtyas Farida Rachmawati, Farida Fatinah, Arik Arubil Hafiedz Maulana Hernowo, Aditya Tri Indrajani, Olly Is Karima Karima, Is Khansa, Shafanony Khoirin Maghfiroh Linda Kartika Dewi Lulut Dwi Nurmamulyosari, Lulut Dwi M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai'i Muhamad Aris Widodo Naqiyah A. Mulachelah Nashi Widodo Noer Hasanah Novinda, Bella Nunung Harijati Nur Jannah Nur Permatasari Nur Permatasari Nuzulul Hikmah Puspitoputri, Alya H. Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rifa’i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rismawati, Ria Rizki Amalia Rochmatika, Lailiyavina Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, Aâ€™liyatur Sakti, Sefihara Paramitha Sapti Puspitarini Sari, Fikriya Novita Sartika, Intan Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Ulinnuha, Maria Wibi Riawan Widodo Widodo Wuragil, Dyah Kinasih Yayu Tsamrotul Fuadah, Yayu Tsamrotul YOGA DWI JATMIKO Yoga Dwi Jatmiko Yuni Kilawati Zahrah, Natasya Adiba Zauhani Kusnul

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