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All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Makara Journal of Science Indonesian Journal of Jamu

Sri Widyarti

Biology Department, Faculty Of Mathematics And Natural Sciences Brawijaya University, Malang Indonesia

Author-ID : 385924

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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The Effect of Calcusolâ„¢ to the Plasma Free Radical and Serum Creatinin in Mus Musculus Nephrolithiasis Model Aâ€™liyatur Rosyidah; Sri Widyarti; Sri Rahayu
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.%x.%x.%x

Calcusol is a traditional medicine (jamu) ade from Tempuyung (Sonchus arvensis) extract and is usually used for cure kidney stone disease. However, there has not been any studies which investigate the its mechanism. The aim of this study is to know the effect of Calcusolâ„¢ to the plasma free radical and serum creatinin of Mus musculus model for renal calcium-oxalate accumulation. This study is carried out by administration of Porang (Amorphophallus muelleri) tuber flour for 3 months with the doses 6 mg/100g BW to induce renal calcium oxalate accumulation and Calcusolâ„¢ treatment for 7 days with the doses of 3.3mg/g BW. Group I was used as a control group. Group II was only given porang (A. muelleri)every day for 3 months. Group III was only given Calcusolâ„¢ for 7 days. Group IV were given porang (A. muelleri) for 3 months then given Calcusolâ„¢ for 7 days. Group V were given porang (A. muelleri) and Calcusolâ„¢ simultaneously for 3 months. Porang and CalcusolTM is administrated orally. Blood was collected from the tail of theanimal for serum creatinin test and plasma free radical test using TBARS method. The data was analyzed using ANOVA followed by Tukey HSD to compare the means employing SPSS 16.0 forwindows. The result of the research shows that the treatment Calcusolâ„¢ on mice model for renal calcium oxalate accumulation. The content of MDA at group I, group II, group III, group IV and group V, respectivelyis 0.81±0.5 mg/ml; 2.63±0.8 mg/ml; 0.56±0.5 mg/ml; 2.09±0.9 mg/ml and 0.17±0.17 mg/ml. And the content of serum creatinine are 0.15±0.04 mg/dL; 0.13±0.03 mg/dL; 0.12±0.08 mg/dL; 0.11±0.016 mg/dL and 0.14±0.015 mg/dL at group I, group II, group III, Group IV, and group V respectively. This indicates that Calcusolâ„¢ decreases plasma free radical production during renal stone formation, while serum creatinin reduced but not significantly changed. Calcusolâ„¢ has an effect to decrease free radical during renalstone formation inmice model for calcium oxalate accumulation and also decrease serum creatinin.

The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model Hafiedz Maulana; Nuzulul Hikmah; Amandia Dewi permana Shita; Nur Permatasari; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment streptozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t-test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides.

Dose Optimization of Calcusolâ„¢ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice (Mus musculus) Ahmad Soni; Muhammad Sasmito Djati; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusolâ„¢ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusolâ„¢ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusolâ„¢ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusolâ„¢ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm.

Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Teguh Suprihatin; Sri Widyarti; Muhaimin Rifa'i; Sri Rahayu
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 Â± 0.26 Ïg/mL) and at day 28 (4.01 Â± 0.26 Ïg/mL).

Peran Puerarin terhadap Aktivitas Intra dan Ekstraseluler pada Kultur Human Umbilical Vein Endothelial Cells (HUVECs) Pasca Induksi Leptin Mochamad Sasmito Djati; Satuman Satuman; Retty Ratnawati; Sri Widyarti; Erly Nur Aisyah; Noer Hasanah; Eko Puji Astuti; Ririn Rochmawati
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Beberapa penelitian terkini menyebutkan bahwa leptin merupakan salah satu penyebab disfungsi endotel yang merupakan salah satu penyebab aterogenesis. Antioksidan puerarin diduga memiliki kemampuan untuk mencegah mekanisme aterogenesis yang distimulasi oleh beberapa sitokin. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah membuktikan dan mengetahui potensi puerarin untuk menghambat ekspresi dan aktivitas intra dan ekstraseluler VCAM-1, PPAR-ɣ, SOD dan H2O2, apoptosis dan nekrosis pada kultur Human Umbilical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng ml-1 leptin. Penelitian ini mempergunakan sel kultur primer HUVECs yang dibagi menjadi empat kelompok perlakuan, yaitu kelompok 0 ng ml-1 dan 0 µM puerarin, kelompok sel yang diinduksi 25 ng ml-1 leptin selama 12 jam, kelompok induksi puerarin 5, 25, 200 dan 525 µM puerarin selama enam jam tanpa leptin, kelompok induksi leptin dan puerarin dengan konsentrasi 5, 25, 200 dan 525 µM selama enam jam. Aktivitas VCAM-1 dan PPAR-ɣ diketahui dengan analisis imunositokimia, metode ELISA digunakan untuk analisis aktivitas SOD dan H2O2. Apoptosis dan nekrosis sel dianalisis setelah HUVECs diberi penanda BrdU selama 20 jam. Data dianalisis dengan analisis satu jalur (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa induksi 25 ng ml-1 dapat meningkatkan ekspresi VCAM-1 (2,68 ± 0,15)% dibandingkan dengan perlakuan 0 ng ml-1 (0,54 ± 0,15)%. Perlakuan induksi puerarin 5, 25, 200, 525 μM memberikan dampak negatif terhadap ekspresi VCAM-1 meskipun pengaruh ini tidak signifikan. Puerarin dapat menekan apoptosis dan nekrosis sel, 525 μM puerarin secara efektif dapat menekan ekspresi PPAR-ɣ. Puerarin tidak memberikan dampak yang signifikan terhadap aktivitas ekstraseluler berdasarkan hasil analisis aktivitas SOD dan H2O2. Kata kunci: apoptosis, H2O2, HUVECs, nekrosis, leptin, puerarin, VCAM-1, PPAR-ɣ, SOD

Effect of Maintenance at Different Salinity against White Spot Syndrome Virus (WSSV) Infection Level in Post Larvae Litopenaeus vannamei Shrimp Attabik Mukhammad Amrillah; Sri Widyarti; Yuni Kilawati
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

White spot syndrime virus (WSSV) is a highly contagious disease in shrimp culture and causing 100% death within 3-10 days of clinical symptoms. WSSV can infect shrimp in post larvae stage (PL) to a size of 40 g. This study was aimed to determine the effect of different salinity against WSSV infection level in post larvae vannamei shrimp (Litopenaeus vannamei). In this study PL 40 size of vannamei shrimp infected with WSSV in virus concentration 20 μg.mL-1 in three different salinity is 5 ppt, 15 ppt, 25 ppt for 4 hours using the soaking method then maintened for 7 days post-infection and then infection level and water quality observed. Data analysis used simple linear regression and F test with a confidence level 95% (P <0.05). The results indicate changes in behavior between healthy and infected shrimp in three levels (mild, moderate, severe). The highest total mild infection in salinity treatment of 25 ppt by 30%, the highest total medium infection at salinity treatment 25 ppt by 37%, the highest total severe infection at salinity treatment 5 ppt by 57%. Shrimp that have mild level of infection are increasing in concominant higher salinity, while the severe infection level has decreases with increasing salinity. Keywords: Infection Level, Litopenaeus vannamei, Post Larvae, Salinity, WSSV

Bioactivity of Sauropus androgynus and Elephantopus scaber to CD4+IL2+ and CD4+IL4+ T Cells Modulation in Balb/c Pregnant Mice Model of Typhoid Yayu Tsamrotul Fuadah; Moch. Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Pregnant woman have higher risk to get infection, because pregnancy decreasing the cell T activity. Sauropus androgynus and Elephantopus scaber has substance like saponin and flavonoid which has been well known as natural imunomodulator, particularly to increase amount of immunocompetent cell. This research is important to recognize effective supplement supply for immunomodulator of S. androgynus and E. scaber to increase mice's (Mus musculus Balb/c) immune system. This research conducted in seven treatments by 3 repetitions for each treatment by using pregnant mice which has been infected by bacteria Salmonella typhimurium (dose 107 CFU.mL-1). Bacteria are injected to mice intraperitoneal in day 5th after giving combination of extract E. scaber and S. androgynus. The dose of E. scaber and S. androgynus combination are 200; 150:37.5; 100:75; 50: 112.5; 150. Five group of treatment were infected by S. typhimurium. Two other groups were the control, namely negative control which was only given NaCMC 0.05% without infection and positive control which was given NaCMC 0.05% and infected by S. typhimurium. After being injected, treatment was redone till the day of surgery. The surgery was executed in day 12th and 18th of pregnancy. Data were analyzed using ANOVA (p < 0.05) and Duncan test. Result indicated that extract of S. androgynus and E. scaber could increase amount of immune system in pregnant mice. This was indicated from significant increasing in amount of cell T CD4+IL2+ and CD4+IL4+ in pregnant mice which has been infected by S. thypimurium. Formula of extract S. androgynus and E. scaber which could return immune condition was approached condition of healthy pregnant mice such as E. scaber 200 mg.kg-1 BW; E. scaber 100 mg.kg-1 BW and S. androgynus 75 mg.kg-1 BW; E. scaber 50 mg.kg-1 BW and S. androgynus 112.5 mg.kg-1 BW; and S. androgynus 150 mg.kg-1 BW, respectively. Keywords: CD4+IL2+,CD4+IL4+, E. scaber, Immunomodulator, S. androgynus, S. typhimurium

Bioactivity of Combination Elephantopus scaber and Sauropus androgynus on the Level of B220 cells of Lymph Node in Pregnant Typhoid BALB/c Mice Lulut Dwi Nurmamulyosari; Muhammad Sasmito Djati; Sri Widyarti
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Elephantopus scaber and Sauropus androgynus are herbal remedies that contain flavonoids as immunomodulatory agents. This study was aimed to observe the changes in the relative number of B cells in lymph node tissue of pregnant typhoid mice. Mice were divided into seven groups K-, K+, E. scaber 200 mg.kg-1 BW, E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW, E. scaber 100 mg.kg-1 BW + S. androgynus 75 mg.kg-1 BW, E. scaber 50 mg.kg-1 BW + S. androgynus 112.5 mg.kg-1 BW, dan S. androgynus 150 mg.kg-1 BW. Mice were dissected on the 12th and 18th day after herbal treatment. Data was analyzed using one way ANOVA (p < 0.05) and Duncan. The result show that E. scaber combination of 200 mg.kg-1 BW and E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW can increase the number of B220 cells (p < 0,05) on pregnant mice typoid model. Keywords: B220, Elephantopus scaber, pregnant, Sauropus androgynus, typhoid fever

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment Rizki Amalia; Sri Widyarti; Moch. Sasmito Djati
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Carcinogen compounds are the compounds that cause DNA damage and trigger cancer. TNF-α and TGF-β are cytokines produced by immune cells and serve to maintain body homeostasis. Elephantopus scaber is a plant that prevents the cancer progression, and improve the body's immune system, so this study was conducted to determine the effect of E. scaber extract on TNF-α and TGF-β after the administration of carcinogen compounds. The study was conducted by administering the carcinogen compound DMBA with a dosage of 0.56 mg kg-1 BW and estradiol with a dose of 0.0504 mg kg-1 BW which was given alternately for 8 weeks to the Mus musculus test animals. The study was conducted in 3 groups: K- (the normal group of mice), K + (a carcinogen-induced group of mice), and P (a group of carcinogen-induced mice and E. scaber extract). The treatment was done in 1 week, 2 weeks, and 3 weeks. Immune cells were isolated from splenocytes and performed immunostaining for flow cytometry analysis. The computed relative amount is TNF-α produced by macrophages CD11b and TGF-β produced by Treg CD4CD25. The relative number of cells was analyzed by two-way ANOVA and advanced Tukey test with 95% confidence level (α = 0.05). The results showed no significant differences in the number of cytokines TNF-α and TGF-β in both the carcinogen-induced mice group and the mice group was given the extract of Elephantopus scaber for 1 week, 2 weeks and 3 weeks. Keywords: Carcinogen, Elephantopus scaber, TNF-α, TGF-β

The Study of Combination Ethanol Extract of Averrhoa bilimbi L. and Momordica charantia L. on CD4+CD25+TGF-β+ Spleenocytes of Hyperglycemia Mice Harits Amrulloh; Bella Novinda; Intan Sartika; Sri Widyarti; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.05

Diabetes mellitus is one of the four priority non-infectious diseases in the world. Plant-based medicine is an alternative treatment with few side effects. Star fruit (Averrhoa bilimbi L.) and bitter melon (Momordica charantia L.) are plants that have anti-hyperglycemic activity. Hyperglycemia produces Reactive Oxygen Species (ROS) that make the β-cells of the pancreas necrosis that decreasing insulin synthesis. The anti-inflammatory activity appears based on the relative levels of CD4+ and CD25+, which are TGF-β-producing regulatory T cells where TGF-β is a mediator that acts as an immunosuppressant. TGF-β would induce CD4 + T lymphocytes into T reg. The purpose of this study is to determine the profile of TGF-β on CD4+ and CD25+ spleenocytes on hyperglycemia mice after ABMC (Averrhoa bilimbi Momordica charantia mix) treatment. Mice were divided into 5 groups, non-diabetic (N), hyperglycemia (H), hyperglycemia with extract doses of 10 mg.kg-1 BW (P1), 40 mg.kg-1 BW (P2), and 160 mg.kg-1 BW (P3). Diabetic mice were obtained after a single injection dose of 145 mg.kg-1 BWÂ streptozotocin (STZ). The result showed that ABMC can reduce blood sugar levels faster and able reduce the number of CD4+TGF-β+ cells in hyperglycemia mice. Keywords: Averrhoa bilimbi L., CD4+TGF-β+, CD25+TGF-β+, hyperglycemia, Momordica charantia L.

Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W Aâ€™liyatur Rosyidah Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Askandar Tjokroprawiro Attabik Mukhammad Amrillah Bella Novinda Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Damayanti, Febriane Eka Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Eko Puji Astuti Erly Nur Aisyah Estri Laras Arumingtyas Farida Rachmawati, Farida Fatinah, Arik Arubil Hafiedz Maulana Harits Amrulloh Intan Sartika Is Karima Jannah, Nur Karima, Is Khoirin Maghfiroh Linda Kartika Dewi Lulut Dwi Nurmamulyosari M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai'i Muhamad Aris Widodo Naqiyah A. Mulachelah Noer Hasanah Nunung Harijati Nur Permatasari Nur Permatasari Nuzulul Hikmah Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Ria Rismawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rizki Amalia Rochmatika, Lailiyavina Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, Aâ€™liyatur Sakti, Sefihara Paramitha Sari, Fikriya Novita Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad Sri Rahayu Sri Rahayu Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Widodo Widodo Yayu Tsamrotul Fuadah, Yayu Tsamrotul Yoga Dwi Jatmiko YOGA DWI JATMIKO Yuni Kilawati Zauhani Kusnul

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