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All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Makara Journal of Science Indonesian Journal of Jamu

Sri Widyarti

Biology Department, Faculty Of Mathematics And Natural Sciences Brawijaya University, Malang Indonesia

Author-ID : 385924

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Histopatologi Hati dan Paru Mencit (Mus musculus) yang Terpapar Formalin dan Benzo(α)pyrene Rike Wahyuningroom; Aris Soewondo; Sri Widyarti
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian formalin dan benzo(α)pyrene terhadap struktur jaringan hati dan paru mencit (Mus musculus). Mencit jantan berumur dua bulan terpapar formalin dosis 2 mg/kg berat badan dengan perlakuan oral selama 60 hari dan terpapar benzo(α)pyrene dosis 250 mg/kg berat badan dengan perlakuan injeksi intraperitoneal sebanyak empat kali setelah 30 hari dengan selang waktu satu hari. Stuktur histologi hati dan paru mencit dianalisis secara deskriptif. Jumlah hepatosit yang mengalami nekrosis dianalisis dengan menggunakan uji Mann-Whitney pada software SPSS 16.0 for Windows. Hasil dari penelitian ini menunjukkan bahwa pemberian formalin, benzo(α)pyrene dan kombinasi antara formalin dan benzo(α)pyrene meningkatkan jumlah hepatosit yang mengalami nekrosis. Nekrosis terjadi di sekitar area portal triad dan di sekitar area vena sentralis pada hati mencit, sedangkan nekrosis terjadi di sekitar area vena pada paru mencit. Kata kunci: benzo(α)pyrene, formalin, hati, nekrosis, paru

Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method Sofy Permana; Sri Widyarti; Serafinah Indriyani; Dian Siswanto; Aris Soewondo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting. Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before. The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.

Uji Kemampuan Antioksidan Ekstrak Etanol dan Kloroform Rumput Laut Gracilaria verrucosa melalui Penghambatan Peroksidasi Lipid Homogenat Hepar Mencit (Mus musculus) Cholilia Abadiatul Masruroh; Sri Widyarti
Biotropika: Journal of Tropical Biology Vol 1, No 6 (2013)
Publisher : University of Brawijaya

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Rumput laut Gracilaria verrucosa merupakan tumbuhan dengan kandungan polifenol yang tinggi seperti rumput laut jenis yang lain sebagai salah satu sumber antioksidan alami untuk menangkap, menghambat dan mencegah aktivitas radikal bebas berlebih di dalam tubuh. Tujuan dari penelitian ini adalah mengetahui kemampuan antioksidan ekstrak ethanol dan kloroform rumput laut Gracillaria verrucosa. Penentuan kadar fenolik dilakukan menggunakan reagen Folin-Cioceltau. Analisis kemampuan antioksidan melalui penghambatan peroksidasi lipid secara in vitro pada homogenat hepar menggunakan uji TBA. Kemampuan antioksidan ekstrak G. verrucosa dibandingkan dengan antioksidan sintetik asam askorbat. Hasil penelitian menunjukkan bahwa kadar fenolik pada ekstrak ethanol dan ekstrak kloroform G. verrucosa yaitu 155,94 dan 146,95 ppm. Kemampuan penghambatan peroksidasi lipid oleh ekstrak etanol dan ekstrak kloroform yaitu 13,59 % dan 12,54 %, sedangkan asam askorbat yatiu 32,54 %. Kata kunci: Gracilaria verrucosa, peroksidasi lipid, TBA

Dampak Stres Salinitas Terhadap Prevalensi White Spot Syndrome Virus (WSSV) dan Survival Rate Udang Vannamei (Litopenaeus vannamei) Pada Kondisi Terkontrol Attabik Mukhammad Amrillah; Sri Widyarti; Yuni Kilawati
Research Journal of Life Science Vol 2, No 2 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

White spot syndrome (WSS) adalah penyakit yang secara signifikan menyebabkan tingginya mortalitas dan kerusakan parah pada budidaya udang. Penelitian ini bertujuan mengetahui dampak stres salinitas terhadap prevalensi WSSV dan survival rate udang vannamei (Litopenaeus vannamei). Penelitian ini menggunakan udang vannamei ukuran PL 40 yang diinfeksi WSSV dengan konsentrasi virus 20 μg/ml pada tiga rentang salinitas yang berbeda 0-10 ppt, 11-20 ppt, 21–30 ppt dan di rendam selama 4 jam kemudian dilakukan pengamatan selama 7 hari pasca infeksi dan diukur survival rate dan kuaitas airnya. Hasil pengamatan menunjukkan bahwa seluruh sampel terinfeksi oleh WSSV, ditunjukkan hasil analisa PCR dan gejala klinis yang timbul. Salinitas 0-10 ppt memberikan hasil persentase survival rate terendah jika dibandingkan dengan rentang salinitas yang lainnya yaitu sebesar 7 ekor atau 33% dari jumlah total sampel yang digunakan. Persentase survival rate udang tertinggi pasca infeksi virus WSSV terdapat pada perlakuan salinitas 21-30 ppt yaitu sebesar 13 ekor atau 63% dari jumlah total individu, dan salinitas 11-20 ppt memiliki persentase survival rate medium yaitu sebesar 10 ekor atau 49% dari jumlah total sampel. Stres salinitas mempengaruhi prevalensi WSSV dengan semakin tingginya tingkat infeksi seiring menurunnya rentang salinitas, akan tetapi survival rate semakin tinggi seiring dengan bertambahnya rentang salinitas.

Korelasi Antara Kadar Lp-PLA2, MDA, F2-Isp di Serum dan Jaringan Aorta dengan Jumlah Sel Busa dalam Proses Aterogenesis pada Tikus Wistar Retno Susilowati; Djanggan Sargowo; Rasjad Indra; Askandar Tjokroprawiro; Sri Widyarti
Jurnal Kardiologi Indonesia Vol. 33, No. 4 Oktober - Desember 2012
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v33i4.288

Background. Atherogenesiswas initiated by cholesterol deposits on foam cell in sub intimae of blood vessel stress oxidation. Atherogenesis in non- hypercolesterolemiausually undergoes an increased level of Lp-PLA2. It, therefore, needs to evaluate the role of Lp-PLA2in the foam cell formation. Aims. To explain the role of Lp-PLA2 in the foam cell forming, to correlate the level of Lp-PLA2 ,MDA, F2-Isp in aorta with foam cell number (FCs) as well as to correlate the level of Lp-PLA2 , MDA, F2-Isp contents in serumwith their contents in aorta and the correlation with FCs.Methods. 30 rats aged 2 months, with their weight averaging from 150-200g,were divided into the control group and the treatment one where the latterwas fed hyperlidemia for 2,8 and 12 weeks. The measurement level of LDL-C,MDA, F2-isp and Lp-PLA2in serum was performedas well as aorta and FCs. Data was analysed using anova, t-Test, path analysis and correlation. Results. Research result indicated that: (1) The level of MDA(a), F2-Isp(s) and Lp-PLA2(s) positively correlated with FCs, Lp-PLA(s)having the highestcorrelation value. (2) Lp-PLA2(a),MDA(s) and F2-Isp(s,a)did not correlate withFCs. (3) There was a positive correlation between Lp-PLA2 with MDA andF2-Isp in both serum and aorta.Conclusion. The enzyme of Lp-PLA2 acts as an activator in forming thefoam cell with stimulated stress oxidation.

The UV-Vis spectrum of antioxidant complex from frozen-dried egg white protein and tomato extract Siti Imama Khoiriyah; Mohammad Mabrur; Sutiman Bambang Sumitro; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In this study, we want to characterize egg white protein-tomato extract complex based on the UV-Vis spectrum. The formulation of antioxidant complex Non-Freeze-drying (NFD) was made at a ratio of 1:10 (v/v) while the Freeze-drying (FD) sample was made at a ratio of 1:2 (v/v). The results showed that the absorption peak of FD complex profile value was lower than the single ingredient, while the tomato extract was higher than the complex profile. On the contrary, the NFD complex showed a higher peak absorption value than to every single ingredient. Based on each UV-Vis spectrum profile, the tomato extract profile shifted the absorption peak, and the absorbance value of the FD sample was changing higher than the NFD sample, while the egg white protein spectrum profile did not change significantly in the FD and NFD samples. The absorption peak of the complex is higher than the single ingredient indicated that the characterization of the complex had been formed. In the NFD complex profile, the absorption peaks formed at the wavelength regions of 230 nm and 280 nm with absorbance values of 2.51 and 0.76 and its number has higher than those of the FD complex with absorbance values of 2.47 and 1.82 at wavelengths of 220 and 265 nm.

EFFECT OF CALCUSOLTM ON CuZnSOD EXPRESSION IN MICE RENAL OF NEPHROLITHIASIS MODEL Arief Azhary; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

This study aimed to determine the effect of traditional medicine, CalcusolTM, on number of cells expressing CuZnSOD in mice renal of nephrolithiasis model. Eight weeks old Swiss strain male mice (Mus musculus) were divided into five groups: (A) control, (B) nephrolithiasis, (C) CalcusolTM, (D) nephrolithiasis & CalcusolTM, and (E) nephrolithiasis & CalcusolTM simultaneously. Nephrolithiasis was induced by applying porang tuber (Amor- phophallus muelleri) flour 0.06 mg/g of body weight during 3 months. CalcusolTM is traditional medicine, made of tempuyung leaves extract with Saccharum lactis as additional substance. The dosage for CalcusolTM treatment was 3.3 mg/g of body weight. After 3 months treatment, the mice were killed by neck-dislocation, the kidneys were isolated and prepared for paraffin histology. CuZnSOD was analyzed by immunohistochemistry (IHC), using rabbit policlonal antibody anti-SOD1 (Bioss, bs-1079R) as primary antibody. Tissues were observed under Olympus BX51, 400x mag- nification. Images were documented with Olympus Digital Camera DP20. The histology images were analized in Immunoratio software online (URL: http://153.1.200.58:8080/immunoratio/) to receive the percentage of number of cells expressing CuZnSOD. The result showed that CalcusolTM administration could decrease number of cells expressing CuZnSOD in kidney significantly (P<0.05). It was supposed that antioxidant content in CalcusolTM could scavenge ROS directly with no induction of CuZnSOD production in cell.

EFFECT OF CALCUSOL TO REDUCE THE CALCIUM CRYSTAL RETENTION IN KIDNEY EPITHELIAL CELLS MODEL OF NEPHROLITHIASIS Ahmad Soni; Moch. Sasmito Djati; Sri Widyarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Kidney stones is a disease that characterized by a disturbance in the bladder. The main constituent of kidney stones namely Calcium Oxalate Monohydrate (COM) crystals. The presence of a COM crystal adhesion to renal tubular cells, will initiate the internalization which will further lead to the formation of crystals retention in the kidney. In Indonesia, there are many herbal products are considered able to cope the complaints due to the kidney stone disease. One of the herbal product is Calcusol „¢, which is the main constituent of those herbal product was the leaf extract of tempuyung. This study observed the effectiveness of Calcusol „¢ in reducing crystals retention that was formed in kidney epithelial cells model of nephrolithiasis. The result showed that Calcusol „¢ is able to reduce the average number of calcium crystals retention in the renal epithelial cells. It indicate that Calcusol „¢ has the ability to reduce crystals retention that already formed in renal epithelial cells. Furthermore, the results of this study are expected to be one of the considerations for further research on the potential of overcoming Calcusol „¢ in kidney stone disease

Microstructure profile on selected-traditional Indonesian herbal powder Naqiyah A. Mulachelah; Syahputra Wibowo; Sri Widyarti; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20224

Jamu is well known as traditional herbal medicine that has been used by Indonesian for centuries ago. Local people usually consume jamu for maintaining health and therapy diseases because it is cheaper and has less side effects. Scientific approach needs to be done to study the characteristic of the herbals. This research aims to characterize Indonesian herbal medicine based on microstructure using Scanning Electron Microscope (SEM). The samples for this research are rosela flower (Hibiscus sabdariffa), ginger (Zingiber officinale), lime fruit (Citrus aurantifolia), and celebes pepper (Piper crocatum). The infusion method preparation was carried out. The mixture was processed further by a freeze-drying method to obtain a powder form. SEM was utilized to capture the herbal powder image. Size of distribution and surface roughness were analysed by ImageJ and Origin Pro 8 on SEM images. The size measurement result showed that infused Rosella has the biggest grain (53.5 µm), followed by lime (43.8 µm), ginger (41.2 µm) and celebes pepper (38.4 µm). The surface roughness results showed that the roughest samples of all is ginger (Ra = 64.417), followed by lime (Ra = 58.761), celebes pepper (Ra = 43.589), and rosella (Ra = 30.855). In conclusion, based on its size measurement result, celebes pepper is the most soluble and has the highest bioavailability, followed by ginger, lime, and rosella. According to its surface roughness, celebes pepper also showed the most soluble compared to other samples, followed by lime, ginger and rosella.

Increasing antioxidant activity of soursop (Annona muricata L.) and noni (Morinda citrifolia L.) leaves fermented by Lactobacillus plantarum BP102 Dayu Nirwana Putri; Sri Widyarti; Yoga Dwi Jatmiko
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20223

Free radicals are constantly produced by either cell metabolism or from external sources. At high concentration, they induced a tissue damage called oxidative stress. Soursop leaf (Annona muricata L.) and noni leaf (Morinda citrifolia L.) are medicinal plants with potency as antioxidants. This study aimed to evaluate the capacity of Lactobacillus plantarum BP102 in elevating the antioxidant activity of soursop and noni leaves. Dried-powder and methanol extract of soursop and noni leaves were diluted with sterile distilled water 3 g/30 mL and 0.3 g/30 mL, respectively, inoculated with 1% (v/v) of L. plantarum BP102 inoculum. The antioxidant activity was carried out using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method. The antioxidant activity increased in dried-powder and methanol extract of soursop and noni leaves with different activity levels after being fermented using L. plantarum BP102 based on IC50. The increasing antioxidant activity in dried-powder of soursop leaves IC50 6.41±0.06 to 0.034±0.01 mg/mL (99.5%) was higher than of the methanol extract IC50 2.78±0.00 to 0.11±0.01 mg/mL (96%). Unfortunately, the effect of fermentation towards noni leaves could only be observed in the form of methanol extract IC50 12.8±0.01 to 0.33±0.02 mg/mL (increased by 97.4%), the dried-powder of noni leaves was suspended and produced a dark color. The probiotic L. plantarum BP102 was used as a fermented agent in increasing the bioactive compounds especially related to antioxidant activity.

Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W Aâ€™liyatur Rosyidah Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Askandar Tjokroprawiro Attabik Mukhammad Amrillah Bella Novinda Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Damayanti, Febriane Eka Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Eko Puji Astuti Erly Nur Aisyah Estri Laras Arumingtyas Farida Rachmawati, Farida Fatinah, Arik Arubil Hafiedz Maulana Harits Amrulloh Intan Sartika Is Karima Jannah, Nur Karima, Is Khoirin Maghfiroh Linda Kartika Dewi Lulut Dwi Nurmamulyosari M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai'i Muhamad Aris Widodo Naqiyah A. Mulachelah Noer Hasanah Nunung Harijati Nur Permatasari Nur Permatasari Nuzulul Hikmah Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Ria Rismawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rizki Amalia Rochmatika, Lailiyavina Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, Aâ€™liyatur Sakti, Sefihara Paramitha Sari, Fikriya Novita Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Widodo Widodo Yayu Tsamrotul Fuadah, Yayu Tsamrotul Yoga Dwi Jatmiko YOGA DWI JATMIKO Yuni Kilawati Zauhani Kusnul

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