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All Journal HAYATI Journal of Biosciences Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Natural B Edu Geography Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Cardiology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Care : Jurnal Ilmiah Ilmu Kesehatan Jurnal Jamu Indonesia Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Makara Journal of Science Indonesian Journal of Jamu

Sri Widyarti

Biology Department, Faculty Of Mathematics And Natural Sciences Brawijaya University, Malang Indonesia

Author-ID : 385924

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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In silico study of active compounds ADMET Profiling in Curcuma xanthorrhiza Roxb and Tamarindus indica as Tuberculosis Treatment Aristyani, Sherry; Nur, M. Irsyad; Widyarti, Sri; Sumitro, Sutiman B.
Jurnal Jamu Indonesia Vol. 3 No. 3 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i3.67

Curcuma xanthorrhiza Roxb and Tamarindus indica L. have been used for a long time by Indonesia local societies as tuberculosis therapy. This study explores the active compounds of Curcuma xanthorrhiza Roxb and Tamarindus indica L that important for suppressing the survival of Mycobacterium tuberculosis and predicts the pharmacokinetics and toxicity of the compounds. stringApp of Cytoscape 3.6.0 was used for screening the compounds targeting mycobacteria proteins, then computational tools like SwissADME (http://swissadme.ch/) and admetSAR (http://lmmd.ecust.edu.cn/admetsar1/predict/) were applied for estimating absorption, distribution, metabolism, excretion, and toxicity (ADMET) of active compounds. The result has been shown that there were some active compounds could target proteins of Mycobacterium tuberculosis. According to the profiling result, these compounds had a various characteristic in gastrointestinal absorption, could pass the blood-brain barrier, and had drug-like properties. In toxicity term, the active compounds did not cause Ames toxicity.

Antibacterial Activity of Pluchea indica Leaf Extract was Increased After Being Fermented with Saccharomyces cerevisiae and Added with its Cell-Free Supernatant Rismawati, Ria; Jatmiko, Yoga Dwi; Widyarti, Sri
Biotropika: Journal of Tropical Biology Vol. 10 No. 2 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.02.04

Multi Drug Resistance (MDR) is a global health problem that endangers public health and can lead to death. Therefore, novel antibacterial agents are required derived from medicinal plants, one of them is beluntas (Pluchea indica) which has high potential as an antibacterial. Fermentation or addition of cell-free supernatant of Saccharomyces cerevisiae is thought to increase the antibacterial content of an herb. This study aimed to evaluate the effect of fermentation and the addition of Cell-Free Supernatant (CFS) of S. cerevisiae to enhance antibacterial activity of P. indica leaf extract. Fresh and dried leaves of beluntas were used in this study. The dried leaves in the form of powder was boiled at 100oC for 45 minutes. Fresh leaves were homogenized by blending. The extract of P. indica was used for fermentation and addition of CFS of S. cerevisiae. CFS as much as 60 mL dan fermentation with cell density of 7.53x105 CFU/mL with 100 mL of P. indica leaf extract were centrifuged at 10,000 rpm for 20 minutes. The antibacterial test method used was the Kirby-Bauer method against Escherichia coli and Staphylococcus aureus. The results showed an increase in antibacterial activity as indicated by the increasing diameter of the inhibition zone either by fermentation or addition of CFS with inhibition zone diameter was 3.85 â€“ 4.81 mm against E. coli and 4.63 â€“ 5.12 mm against S. aureus. The fermented P. indica and addition of CFS was shown to be potentially developed as antibacterial agents.Â Keywords: Antibacterial, CFS, fermentation, Pluchea indica, Saccharomyces cerevisiae

Hepatoprotection and Immunomodulation of Natural Killer and CD8 T Cells by Meniran-Turmeric Extract Combination in Mice Injected with 7,12-Dimethylbenz[a] Anthracene Puspitarini, Sapti; Widodo, Nashi; Widyarti, Sri; Azzamuddin, Haidar; Ulinnuha, Maria; Puspitoputri, Alya H.; Khansa, Shafanony; Rifa’i, Muhaimin
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.499-507

Combination herbal or polyherbal offers advantages by synergizing multiple plants to address health issues more effectively. Meniran and turmeric are traditionally used medicinal plants with notable pharmacological activities due to their bioactive compounds. This study investigated the curative effect of a meniran-turmeric extract combination on immunocompetent cells, particularly NK and CD8 cells, and on liver histopathology in mice administered 7,12-dimethylbenz[a]anthracene (DMBA). Female BALB/c mice (Mus musculus) were injected subcutaneously with DMBA at 45 mg/kg body weight (BW) in the mammary gland area and maintained for eight weeks. They were then treated with a 1:1 meniran-turmeric extract combination for two weeks at doses of 100, 300, 900, and 1,800 mg/kg BW, followed by liver and immune cell analyses. The results showed a lower CD8+ T-cell population in extract-treated groups (17.53±1.13%-19.79±1.51%) compared with the DMBA group (20.92±4.74%). Conversely, NK+ cell populations increased after extract treatment (0.33±0.01%-0.56±0.13%) compared with DMBA alone (0.27±0.02%). Liver histopathology indicated that extract combination administration did not cause more severe damage than that observed in the DMBA group. An extract combination of meniran and turmeric could modulate the immune system, particularly NK and CD8 cells, in mice injected with DMBA. In addition, the study indicated that the combination of meniran and turmeric extract might play a role as an antihepatotoxic agent. This research could serve as a consideration for future studies developing a combination formulation of meniran and turmeric.

Peran Puerarin terhadap Aktivitas Intra dan Ekstraseluler pada Kultur Human Umbilical Vein Endothelial Cells (HUVECs) Pasca Induksi Leptin Djati, Mochamad Sasmito; Satuman, Satuman; Ratnawati, Retty; Widyarti, Sri; Aisyah, Erly Nur; Hasanah, Noer; Astuti, Eko Puji; Rochmawati, Ririn
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Graduate School, Universitas Brawijaya

Beberapa penelitian terkini menyebutkan bahwa leptin merupakan salah satu penyebab disfungsi endotel yang merupakan salah satu penyebab aterogenesis. Antioksidan puerarin diduga memiliki kemampuan untuk mencegah mekanisme aterogenesis yang distimulasi oleh beberapa sitokin. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah membuktikan dan mengetahui potensi puerarin untuk menghambat ekspresi dan aktivitas intra dan ekstraseluler VCAM-1, PPAR-ɣ, SOD dan H2O2, apoptosis dan nekrosis pada kultur Human Umbilical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng ml-1 leptin. Penelitian ini mempergunakan sel kultur primer HUVECs yang dibagi menjadi empat kelompok perlakuan, yaitu kelompok 0 ng ml-1 dan 0 µM puerarin, kelompok sel yang diinduksi 25 ng ml-1 leptin selama 12 jam, kelompok induksi puerarin 5, 25, 200 dan 525 µM puerarin selama enam jam tanpa leptin, kelompok induksi leptin dan puerarin dengan konsentrasi 5, 25, 200 dan 525 µM selama enam jam. Aktivitas VCAM-1 dan PPAR-ɣ diketahui dengan analisis imunositokimia, metode ELISA digunakan untuk analisis aktivitas SOD dan H2O2. Apoptosis dan nekrosis sel dianalisis setelah HUVECs diberi penanda BrdU selama 20 jam. Data dianalisis dengan analisis satu jalur (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa induksi 25 ng ml-1 dapat meningkatkan ekspresi VCAM-1 (2,68 ± 0,15)% dibandingkan dengan perlakuan 0 ng ml-1 (0,54 ± 0,15)%. Perlakuan induksi puerarin 5, 25, 200, 525 μM memberikan dampak negatif terhadap ekspresi VCAM-1 meskipun pengaruh ini tidak signifikan. Puerarin dapat menekan apoptosis dan nekrosis sel, 525 μM puerarin secara efektif dapat menekan ekspresi PPAR-ɣ. Puerarin tidak memberikan dampak yang signifikan terhadap aktivitas ekstraseluler berdasarkan hasil analisis aktivitas SOD dan H2O2. Kata kunci: apoptosis, H2O2, HUVECs, nekrosis, leptin, puerarin, VCAM-1, PPAR-ɣ, SOD

Adaptive Immune Response Stimulation on Nephrolithiasis Mice Model after Treatment of Tempuyung (Sonchus arvensis L.) Leaf Extract Maghfiroh, Khoirin; Rifa'i, Muhaimin; Widyarti, Sri
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Graduate School, Universitas Brawijaya

Calcium crystal accumulation on kidney can cause kidney stone (nephrolithiasis). The oxalate calcium crystal which is deposite on the kidney can trigger inflammation on the epithelial that is able to induce cells death (necrosis). The necrosis is able to cause inflammation and it will affect the body's immune system. Infection agent that comes to the body will be responded by the innate immunity which will be responded later by adaptive immunity. One of herbal agent that is expected to be used to stimulate adaptive immunity response is Tempuyung (Sonchus arvensis) leaf extract. The aim of this study is to find out the change of relative amount of CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after S.arvensis leaf extract. The mice are divided into six groups; control group, placebo, nephrolithiasis, S.arvensis leaf extract for 7 days, nephrolithiasis then it is continued with S.arvensis leaf extract for 7 days, and simultaneous (nephrolithiasis and S.arvensis leaf extract for 3 months). The amount of relative T lymphocyte cells is measured by using BD FACSCalibur FlowcytometerTM. The data is analyzed by using ANOVA one way (p<0.05) using SPSS 16.0 software for Windows. The result shows that there are changes of relative CD4+ T cells, CD8+ T cells, and B (B220) cells on nephrolithiasis mice model after the giving of S.arvensis leaf extract. The treatment of S.arvensis leaf extract on the nephrolithiasis mice model can stimulate the homeostatic activity by suppressing the B cells. Compound of S.arvensis leaf extract that can inhibit of Th1 cells and the increase of Th2 cells by proliferation cells activity. The treatment of S.arvensis leaf extract for 7 days can suppress CD4+. The S.arvensis leaf extract can stimulate adaptive immune response which is caused by immunomodulatory active component.Keywords: Nephrolithiasis, Sonchus arvensis L., leaf extract, adaptive immune response.

Effect of Maintenance at Different Salinity against White Spot Syndrome Virus (WSSV) Infection Level in Post Larvae Litopenaeus vannamei Shrimp Amrillah, Attabik Mukhammad; Widyarti, Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

White spot syndrime virus (WSSV) is a highly contagious disease in shrimp culture and causing 100% death within 3-10 days of clinical symptoms. WSSV can infect shrimp in post larvae stage (PL) to a size of 40 g. This study was aimed to determine the effect of different salinity against WSSV infection level in post larvae vannamei shrimp (Litopenaeus vannamei). In this study PL 40 size of vannamei shrimp infected with WSSV in virus concentration 20 μg.mL-1 in three different salinity is 5 ppt, 15 ppt, 25 ppt for 4 hours using the soaking method then maintened for 7 days post-infection and then infection level and water quality observed. Data analysis used simple linear regression and F test with a confidence level 95% (P <0.05). The results indicate changes in behavior between healthy and infected shrimp in three levels (mild, moderate, severe). The highest total mild infection in salinity treatment of 25 ppt by 30%, the highest total medium infection at salinity treatment 25 ppt by 37%, the highest total severe infection at salinity treatment 5 ppt by 57%. Shrimp that have mild level of infection are increasing in concominant higher salinity, while the severe infection level has decreases with increasing salinity. Keywords: Infection Level, Litopenaeus vannamei, Post Larvae, Salinity, WSSV

Bioactivity of Sauropus androgynus and Elephantopus scaber to CD4+IL2+ and CD4+IL4+ T Cells Modulation in Balb/c Pregnant Mice Model of Typhoid Fuadah, Yayu Tsamrotul; Djati, Moch. Sasmito; Widyarti, Sri
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Pregnant woman have higher risk to get infection, because pregnancy decreasing the cell T activity. Sauropus androgynus and Elephantopus scaber has substance like saponin and flavonoid which has been well known as natural imunomodulator, particularly to increase amount of immunocompetent cell. This research is important to recognize effective supplement supply for immunomodulator of S. androgynus and E. scaber to increase mice's (Mus musculus Balb/c) immune system. This research conducted in seven treatments by 3 repetitions for each treatment by using pregnant mice which has been infected by bacteria Salmonella typhimurium (dose 107 CFU.mL-1). Bacteria are injected to mice intraperitoneal in day 5th after giving combination of extract E. scaber and S. androgynus. The dose of E. scaber and S. androgynus combination are 200; 150:37.5; 100:75; 50: 112.5; 150. Five group of treatment were infected by S. typhimurium. Two other groups were the control, namely negative control which was only given NaCMC 0.05% without infection and positive control which was given NaCMC 0.05% and infected by S. typhimurium. After being injected, treatment was redone till the day of surgery. The surgery was executed in day 12th and 18th of pregnancy. Data were analyzed using ANOVA (p < 0.05) and Duncan test. Result indicated that extract of S. androgynus and E. scaber could increase amount of immune system in pregnant mice. This was indicated from significant increasing in amount of cell T CD4+IL2+ and CD4+IL4+ in pregnant mice which has been infected by S. thypimurium. Formula of extract S. androgynus and E. scaber which could return immune condition was approached condition of healthy pregnant mice such as E. scaber 200 mg.kg-1 BW; E. scaber 100 mg.kg-1 BW and S. androgynus 75 mg.kg-1 BW; E. scaber 50 mg.kg-1 BW and S. androgynus 112.5 mg.kg-1 BW; and S. androgynus 150 mg.kg-1 BW, respectively. Keywords: CD4+IL2+,CD4+IL4+, E. scaber, Immunomodulator, S. androgynus, S. typhimurium

Bioactivity of Combination Elephantopus scaber and Sauropus androgynus on the Level of B220 cells of Lymph Node in Pregnant Typhoid BALB/c Mice Nurmamulyosari, Lulut Dwi; Djati, Muhammad Sasmito; Widyarti, Sri
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Elephantopus scaber and Sauropus androgynus are herbal remedies that contain flavonoids as immunomodulatory agents. This study was aimed to observe the changes in the relative number of B cells in lymph node tissue of pregnant typhoid mice. Mice were divided into seven groups K-, K+, E. scaber 200 mg.kg-1 BW, E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW, E. scaber 100 mg.kg-1 BW + S. androgynus 75 mg.kg-1 BW, E. scaber 50 mg.kg-1 BW + S. androgynus 112.5 mg.kg-1 BW, dan S. androgynus 150 mg.kg-1 BW. Mice were dissected on the 12th and 18th day after herbal treatment. Data was analyzed using one way ANOVA (p < 0.05) and Duncan. The result show that E. scaber combination of 200 mg.kg-1 BW and E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW can increase the number of B220 cells (p < 0,05) on pregnant mice typoid model. Keywords: B220, Elephantopus scaber, pregnant, Sauropus androgynus, typhoid fever

The Immunomodulatory Effect of Elephantopus scaber and Sauropus androgynus Extract to Cellular Immune Response in Pregnant Mus muscullus Infected by Salmonella typhimurium Jannah, Nur; Djati, Moch. Sasmito; Widyarti, Sri
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Pregnancy has a high risk and is more susceptible to infectious diseases. E. scaber and S. androgynus contains saponins and flavonoids as an immunomodulatory that can increase the body's immunity. The purpose of this study is to determine the immunomodulatory effects of E. scaber and S. androgynus leaf extract against cellular immune response in pregnant mice infected S. typhimurium. This research used seven treatment groups including negative control (K-) mice without injected S. typhimurium, positive control (K+) mice were injected S. typhimurium and extract treatment with 5 variant doses and dissected on the 12th and 18th days. Lymphocytes was isolated from the blood, then relative number of CD4+ and CD8+ were analyzed with flow cytometry and data continued with ANOVA. Statistical analysis showed that different extract dose and duration of treatment had a significant effect on the number of CD4+ and CD8+ T cells. E. scaber and S. androgynus act as immunomudulatory effect and treatment with combination of extracts E. scaber and S. androgynus 50 mg.kg-1 BW + 112.5 mg.kg-1 BW respectively and treatment with extract of S. androgynus 150 mg.kg-1 BW are the optimum treatment which can restore immune system conditions such as normal pregnancy without infection.Keywords: Cellular Immune, E. scaber, Immunomodulatory, S. androgynus, S. typhimurium.

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment Amalia, Rizki; Widyarti, Sri; Djati, Moch. Sasmito
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Carcinogen compounds are the compounds that cause DNA damage and trigger cancer. TNF-α and TGF-β are cytokines produced by immune cells and serve to maintain body homeostasis. Elephantopus scaber is a plant that prevents the cancer progression, and improve the body's immune system, so this study was conducted to determine the effect of E. scaber extract on TNF-α and TGF-β after the administration of carcinogen compounds. The study was conducted by administering the carcinogen compound DMBA with a dosage of 0.56 mg kg-1 BW and estradiol with a dose of 0.0504 mg kg-1 BW which was given alternately for 8 weeks to the Mus musculus test animals. The study was conducted in 3 groups: K- (the normal group of mice), K + (a carcinogen-induced group of mice), and P (a group of carcinogen-induced mice and E. scaber extract). The treatment was done in 1 week, 2 weeks, and 3 weeks. Immune cells were isolated from splenocytes and performed immunostaining for flow cytometry analysis. The computed relative amount is TNF-α produced by macrophages CD11b and TGF-β produced by Treg CD4CD25. The relative number of cells was analyzed by two-way ANOVA and advanced Tukey test with 95% confidence level (α = 0.05). The results showed no significant differences in the number of cytokines TNF-α and TGF-β in both the carcinogen-induced mice group and the mice group was given the extract of Elephantopus scaber for 1 week, 2 weeks and 3 weeks. Keywords: Carcinogen, Elephantopus scaber, TNF-α, TGF-β

Co-Authors A'liyatur Rosidah A.A. Ketut Agung Cahyawan W Aâ€™liyatur Rosyidah Aditya Tri Hernowo Ahmad Lubab Ahmad Soni Ahmad Soni Amandia Dewi Permana Shita Amrulloh, Harits Apik Budi Santoso Arief Azhary Aris Soewondo Aris Suwondo Aristyani, Sherry Askandar Tjokroprawiro Attabik Mukhammad Amrillah Aulanni'am Azzamuddin, Haidar Cholilia Abadiatul Masruroh Cholilia Abadiatul Masruroh Damayanti, Febriane Eka Daniar Kusumawati Dayu Nirwana Putri Dewi Parlina Ningrum Dian Siswanto Djanggan Sargowo Dyah Kinasih Wuragil Eko Puji Astuti Erly Nur Aisyah Estri Laras Arumingtyas Farida Rachmawati, Farida Fatinah, Arik Arubil Hafiedz Maulana Is Karima Karima, Is Khansa, Shafanony Khoirin Maghfiroh Linda Kartika Dewi Lulut Dwi Nurmamulyosari, Lulut Dwi M. Irsyad Nur Maftuch Maftuch Marji Marji Moch Sasmito Djati Mochammad Pratama Viadi Mohammad Mabrur Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifa'i Muhaimin Rifai'i Muhamad Aris Widodo Naqiyah A. Mulachelah Nashi Widodo Natasya Adiba Zahrah Noer Hasanah Novinda, Bella Nunung Harijati Nur Jannah Nur Permatasari Nur Permatasari Nuzulul Hikmah Olly Indrajani Puspitoputri, Alya H. Radita Intan Aisyah Pratiwi Rasjad Indra Retno Susilowati Retty Ratnawati Rifa'i, Muhaimin Rifa'i, Muhaimin Rifa’i, Muhaimin Rike Wahyuningroom Rike Wahyuningroom Ririn Rochmawati Rismawati, Ria Rizki Amalia Rochmatika, Lailiyavina Rodiyati Azrianingsih Rodiyati Azrianingsih Rosidah, A'liyatur Rosyidah, Aâ€™liyatur Sakti, Sefihara Paramitha Sapti Puspitarini Sari, Fikriya Novita Sartika, Intan Satuman Satuman Serafinah Indriyani Sherry Aristyani Siti Imama Khoiriyah Sofy Permana Soni, Ahmad Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu Sri Rahayu SRI RAHAYU Suroso Suroso Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman B. Sumitro Sutiman Bambang Sumitro Suwondo, Aris Syahputra Wibowo Teguh Suprihatin Teguh Suprihatin Ulinnuha, Maria Wibi Riawan Widodo Widodo Yayu Tsamrotul Fuadah, Yayu Tsamrotul YOGA DWI JATMIKO Yoga Dwi Jatmiko Yuni Kilawati Zauhani Kusnul

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