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All Journal Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Indonesian Journal of Cancer Chemoprevention Adi Widya : Jurnal Pengabdian Masyarakat Jurnal Farmasi Medica (Pharmacy Medical Journal) PMJ Media Ilmu Kesehatan JFIOnline Jurnal Mandala Pharmacon Indonesia Science Midwifery Indonesian Journal of Global Health research Formosa Journal of Multidisciplinary Research (FJMR) Riset Informasi Kesehatan Journal of Health Management and Pharmacy Exploration (JOHMPE) Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Titik Sunarni
Faculty of Pharmacy Setia Budi University Jl. Letjen Sutoyo Mojosongo – Solo, 57127, Phone. 0271-852518, HP:08121520941
Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Uji Aktivitas Antibakteri dan Antibiofilm Fraksi Ekstrak Daun Gamal (Gliricidia Sepium (Jacq Walp)) Terhadap Staphylococcus Aureus ATCC 25923 Herman, Herman; Sunarni, Titik; Saptarini, Opstaria
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 1 (2024): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i1.484

Abstract

Daun gamal (Gliricidia sepium (Jacq Walp)) di dalamnya terkandung zat flavonoid, alkaloid, tanin, saponin, dan terpenoid. Senyawa dalam daun gamal mempunyai sifat uang antibakteri. Tujuan dari penelitian ini yakni untuk mengetahui bagaimana peran ekstrak serta fraksi n-heksana, etil asetat, serta air terhadap aktivitas antibakteri dengan menghambat pembentukan dan degradasi biofilm. Metode maserasi digunakan untuk mengekstraksi daun gamal menggunakan pelarut etanol 96%. Ekstrak kental dilakukan fraksinasi dengan pelarut n-heksana, air, serta etil asetat. Uji aktivitas antibakteri dilaksanakan melalui metode dilusi dilanjutkan observasi pengaruh kerja melalui uji AAS kebocoran ion. Uji aktivitas antibiofilm menggunakan alat microplate serta pembacaannya dilaksanakan pada panjang gelombang 595 nm. Hasil penelitian bahwa fraksi etil asetat daun gamal teraktif terhadap bakteri S. aureus. Fraksi etil asetat daung gamal  dapat merusak pada membran  sel bakteri. Rata-rata persentase penghambatan pembentukan biofilm terhadap S. aureus terbesara di konsentrasi 50%, yaitu fraksi etil asetat sebanyak 40,69%, ekstrak 37,51%, fraksi n-heksana 28,93% dan fraksi air 30,20%, sedangkan rata-rata persentase degradasi biofilm terlihat yang tertinggi adalah fraksi etil asetat dengan konsentrasi 50% yaitu sebesar 37,27%, ekstrak  34,55%, fraksi air 31,77%, fraksi n-heksan nilai 30,05%. Kontrol positif (Tetrasiklin) memiliki aktivitas dengan nilai 51,66 %.
Test for Inhibition of the Enzyme Α-Glucosidase Fraction of Ethanol Extract of Onco Leaves (Spondias pinnata (L.f.) Kurz.) and the Antidiabetic Activity of the Selected Fraction Pagal, Fajria Rezky Priyanti Amara; Sunarni, Titik; Supriyadi, Supriyadi
Indonesian Journal of Global Health Research Vol 6 No 3 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6i3.3166

Abstract

Degenerative diseases are the number one disease in Southeast Asia. Based on WHO data in 2008, the death rate in Southeast Asia was around 14.5 million, around 55% (7.9 million) caused by degenerative diseases. One of the degenerative diseases is diabetes mellitus. Diabetes mellitus is a metabolic disorder characterized by hyperglycemy. Research that has been carried out to look at the antidiabetic activity of onco (Spondias pinnata (L.f.) Kurz) leaves is still limited to the extraction results. Objectives: This study aims to determine the fraction of onco leaf ethanol extract in inhibiting the enzyme alpha glucosidase, having antidiabetic activity and histopathological picture of pancreatic cells in streptozotocin-induced male white rats (Rattus norvegicus). This study used inhibition testing of the enzyme α-glucosidase fraction of onco leaf ethanol extract with concentrations of 12.5, 25, 50,100 and 200 μg/mL using a microplate reader with a wavelength of 405 nm, as well as with experimental laboratory test animal methods as many as 28 mice divided into 7 treatment groups the induced streptozotocin except the normal group. Group I as normal control of rats that were only given regular drinking food, group II as negative control without treatment, group III as positive control using glibenclamide drugs and group IV ethanol extract 500, V n-hexane fraction 132.54, VI ethyl acetate fraction 126.24 and VII water fraction 239 mg/KgBB treatment given for 14 days. The results showed that onco leaf ethanol extract inhibits the enzyme α-glucosidase with very strong strength (IC50 = 39.13 μg / ml) and the n-hexane fraction has the effect of reducing glucose levels as well as the ability to correct the histopathology of the pancreas of male white rats (Rattus norvegicus) induced streptozotocin. Conclusions: Ethanol extract and the fraction of onco leaves (Spondias pinnata (L.f.) Kurz) has antidiabetic activity with a mechanism of action inhibiting the enzyme alpha glucosidase and the activity of decreasing blood glucose levels and has the ability to correct the histopathology of the pancreas of male white rats (Rattus norvegicus) induced streptozotocin.
Antifertility Activity and Chemical Content of Moringa Oleifera Lam Plant Extract on The Reproductive System of Male and Female White Rats Munawir, Munawir; Sunarni, Titik; Saptarini, Opstaria
Indonesian Journal of Global Health Research Vol 6 No S6 (2024): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v6iS6.4821

Abstract

Research shows that Moringa extract possesses anti-fertility effects on the reproductive systems of male and female rats. This study investigates Moringa's active chemical compounds and their impact on reproductive parameters such as sperm concentration, motility, morphology, and seminiferous tubule diameter, along with hormone levels (progesterone, LH, FSH), estrogenic activity, and the estrous cycle. The research, a literature review, compiles findings from quality international (Scopus) and national (SINTA) journals, Translation and proofreading: there are 8 articles that meet the research inclusion criteria, selected within a range from the year 2010 to 2024. Key active compounds identified in Moringa include alkaloids, tannins, flavonoids (kaempferol and quercetin), and benzyl isothiocyanate, all of which demonstrate anti-fertility effects. These compounds were observed to reduce sperm concentration, motility, and morphology, and to decrease seminiferous tubule diameter in males, while also impacting hormone levels and the estrous cycle in females. The findings support Moringa’s potential as an anti-fertility agent, highlighting its impact across various reproductive parameters in both genders.
Optimization and Characterization of Self Nano Emulsifying Drug Delivery System (SNEDDS) Naringenin Components with D-Optimal Mixture Design Method and Anti-Aging Test In Vivo Taena, Adriani; Kuncahyo, Ilham; Sunarni, Titik
Formosa Journal of Multidisciplinary Research Vol. 3 No. 9 (2024): September 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/fjmr.v3i9.11236

Abstract

Naringenin (5,7-dihydroxy-2-(4-hydroxyphenyl) chroman-4-one) is one of the flavanone aglycone compounds that has the main activity as an antioxidant. The purpose of this study was to determine the optimum formula and characterization of naringenin SNEDDS and to see the anti-aging activity of the optimum formula of naringenin SNEDDS. The optimum formula of naringenin SNEDDS was obtained by a combination of oil components, surfactants and cosurfactants (miglyol, tween 80 and transcutol) using the D-optimal mixture design method. The characteristics of naringenin SNEDDS are seen based on parameters such as drug loading, emulsification time, % transmittance, particle size analyzer (PSA) and zeta potential. The results of the optimum formula characterization test of naringenin SNEDDS obtained drug loading of 2.004507 mg/5 ml, emulsification time of 13.07 seconds, percent transmittance of 85.1%, particle size of 78.76 nm and zeta potential of -8.14 mV. Based on the results of the characteristics obtained from the optimum formula, the naringenin SNEDDS preparation meets the characteristics of good SNEDDS and has the potential to be developed into a drug preparation.
Antibacterial and Antibiofilm Activity of Katuk Leaf Fraction (Sauropus androgynus (L.) Merr.) against Bacteria Staphylococcus Aureus ATCC 25923 Khairunnisa, Rizky Dila; Sunarni, Titik; Saptarini, Opstaria
Formosa Journal of Multidisciplinary Research Vol. 3 No. 9 (2024): September 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/fjmr.v3i9.11237

Abstract

This study aims to determine the antibacterial and antibiofilm activity of extracts and fractions from katuk leaves against Staphylococcus aureus. Katuk leaves were extracted using the maceration process and fractionated using the liquid-liquid method with solvents such as n-hexane, ethyl acetate, and water. Diffusion and dilution methods were used to measure the antibacterial activity, and crystal violet staining at a wavelength of 595 nm was used to measure the inhibition and biofilm destruction activities. The antibacterial and antibiofilm test results obtained were analyzed using ANOVA statistical test. The results of the diameter of the inhibition zone with a concentration of 75 mg/ml showed that the extract was 11.3 ± 1.02 mm, the ethyl acetate fraction was 18.1 ± 2.46 while the water and n-hexane fractions were 0.0 ± 0.0 mm. The antibiofilm test results showed inhibitory activity against Staphylococcus aureus bacteria with the average IC50 values obtained from extracts, n-hexane, ethyl acetate, and water fractions were 10.248±0.405; 11.799±0.355; 8.332±0.585; and 11.372±0.557. While the average EC50 values obtained from the extract, n-hexane, ethyl acetate, and water fractions were 9.343±0.586; 10.530±0.483; 7.695±0.491; and 8.490±0.954.
Uji Sitotoksik Ekstrak dan Fraksi Daun Dollu (Dodonaea viscosa Jacq.) terhadap Sel Kanker Payudara T47D serta Ekspresi Protein p53 dan Bcl-2 Susilowati, Rizka Agustine; Sunarni, Titik; Indrayati, Ana
Jurnal Mandala Pharmacon Indonesia Vol. 11 No. 2 (2025): Jurnal Mandala Pharmacon Indonesia 
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v11i2.837

Abstract

Kanker payudara merupakan penyebab utama kematian akibat kanker di dunia, dengan lebih dari 396.000 kasus per tahun. Tanaman Dollu (Dodonaea viscosa Jacq) diketahui memiliki sifat antibakteri, namun potensinya sebagai agen antikanker belum banyak diteliti. Penelitian ini bertujuan untuk mengevaluasi aktivitas sitotoksik ekstrak dan fraksi daun Dollu terhadap sel kanker T47D, menilai selektivitasnya, dan menyelidiki efeknya terhadap ekspresi protein p53 dan Bcl-2 pada sel kanker tersebut. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96% selama tiga hari. Ekstrak dan fraksi yang dihasilkan (etanol, air, etil asetat, dan n-heksan) dianalisis untuk senyawa kimia, termasuk flavonoid, tanin, alkaloid, saponin, steroid, dan terpenoid. Uji sitotoksik dilakukan menggunakan metode MTT, diikuti dengan uji selektivitas, dan ekspresi protein p53 serta Bcl-2 dianalisis menggunakan imunositokimia. Hasil penelitian menunjukkan bahwa fraksi air menunjukkan aktivitas sitotoksik paling efektif dengan nilai IC50 139,84 ± 13 µg/mL. Ekstrak dan fraksi menunjukkan nilai selektivitas >2. Selain itu, fraksi air secara signifikan meningkatkan ekspresi protein p53 dengan nilai EC50 44,41 ± 7,09 µg/mL, yang menunjukkan hasil signifikan pada uji ANOVA (nilai p 0,000 < 0,05). Sebaliknya, fraksi air secara signifikan menurunkan ekspresi Bcl-2 dengan nilai EC50 193,22 ± 20,99 µg/mL, yang menunjukkan hasil signifikan pada uji Kruskal-Wallis (nilai p 0,022 < 0,05).
Literature review of the cytotoxic activity of faloak (sterculia quaddrifida r.br) against t47d and mcf-7 breast cancer cells and its active compounds Seran, Rumaldus; Herdwiani, Wiwin; Sunarni, Titik
Science Midwifery Vol 13 No 6 (2026): February: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v13i6.2246

Abstract

Faloak (Sterculia quadrifida R.Br) is one of the plants used by the community as a multipurpose drug. Faloak bark herb has long been used by the people of East Nusa Tenggara as a medicinal plant. Empirically, boiled water from the bark of the faloak plant is used by the people of East Nusa Tenggara as a cure for hepatitis, typhus, ulcers, and stamina recovery. The data extraction method used is narrative by grouping similar data from the literature review results according to the size of the results to be presented. The flow of the journal search is about testing the cytotoxic activity of faloak (Sterculia quadrifida R.Br) against T47D and MCF-7 breast cancer cells and the content of their active compounds. From these data, screening, journal eligibility, and monitoring of conformity with inclusion and exclusion criteria were carried out to obtain appropriate journals to conclude the cytotoxic activity of faloak. The results of a literature review showed that faloak (Sterculia quadrifida R.Br) had cytotoxic activity against T47D and MCF-7 breast cancer cells. Chemical compounds that have cytotoxic activity are alkaloids, flavonoids, terpenoids, tannins, naphthoquinone, apigenin, and puerarin. Naptaquinone derivatives 2,3-dihydro-6hydroxy-2-methylenenaphtho [1,2-b] furan-4,5-dione and 2-iminoethyl 2-(2-(1-hydroxypentan-2-yl) phenyl) acetate obtained from the extract or active fraction of faloak bark (Sterculia quadrifida R.Br) showed cytotoxic activity against T47D breast cancer cells. Apigenin compounds were found to be the most effective phytoestrogens in inhibiting the growth of breast cancer cells. Apigenin also has apoptotic effects and induces autophagy in breast cancer cells. an effective proteasome inhibitor in breast cancer cell culture and breast cancer xenografts, and puerarin can increase DUSP1 expression by increasing the expression level of miR-133a-3p in breast cancer cells.
Co-Authors Ahmad Suriyadi Muslim Ana Indrayati Daniel Happy Putra Doso, Tri Efrilia, Erin Fransiska Leviana Ika Purwidyaningrum Ilham Kuncahyo, Ilham Khairunnisa, Rizky Dila Mardiana, Lia Mardiyono Mardiyono, . Munawir Munawir Murukmihadi, Mimiek Opstaria saptarini Pacheco, Mergita Angela Pagal, Fajria Rezky Priyanti Amara Reslely Harjanti Rini Prastiwi Seran, Rumaldus Siti Aisiyah Suhartinah, Suhartinah Supriyadi Supriyadi Susilowati, Rizka Agustine Taena, Adriani Tri Murti Andayani Vivin Nopiyanti Wiwin Herdwiani Yudi Rinanto