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Journal : Jurnal Katalisator

The AKTIVITAS ANTIDIABETES EKSTRAK KULIT JERING (PITHECELLOBIUM JIRINGA) DENGAN FRAKSI PELARUT METANOL Alfin Surya; Dimas Prada Sumitra; Hesti Marliza; Zaiyar Zaiyar
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1350.877 KB) | DOI: 10.62769/katalisator.v6i1.265

Abstract

ABSTRACT Jering skin is a solid waste that can cause problems if not treated seriously because it pollutes the environment. Based on previous research, the potential possessed by jering skin is very high because it contains flavonoids and polyphenols which are antiseptic, and antioxidant, also has a very toxic toxicity value for shrimp artemia salina, this has the potential as an anticancer. Therefore, this study aims to continue testing other activities, namely by conducting antidiabetic activity tests using the methanol solvent fraction. The method used is to use a Microplate reader or platec reader at a wavelength of 410 nm to determine absorbance, so that with data analysis the Inhibitor Concentration value of 50% (IC50) of sample extracts is inhibited by the α-glucosidase enzyme in hydrolyzing the p-nitrophenyl substrate. -α-D-glucopyranoside (p-NPG) to form glucose. The results of the study obtained an IC50 value of 387,6091 µg/mL. When compared with IC50 values ​​for positive root as a positive control of 0.8135 μg / mL, it is still weak because the root is a pure composition while the Methanol Faction still contains Rough extract still in the fractionation but the dry skin Methanol fraction can be needed as a source to be used as a substitute for further as a joint exploration of antidiabetic in jering skin. Keywords: Jering, Methanol, Antidiabeti, IC50. ABSTRAK Kulit jering merupakan limbah padat yang dapat menimbulkan masalah bila tidak ditangani dengan serius karena mencemari lingkungan. Berdasarkan penelitian sebelumnya, potensi yang dimiliki oleh kulit jering tersebut sangat tinggi karena mengandung senyawa flavonoid dan polifenol yang bersifat antiseptik, dan antioksidan, juga memiliki nilai Toksisitas yang sangat toksit terhadap udang artemia salina hal ini berpotensi sebagai antikanker. Oleh karena itu, penelitian ini bertujuan untuk melanjutkan uji aktivitas lainnya yaitu dengan melakukan uji aktivitas Antidiabetes mengunakan fraksi pelarut metanol. Adapun metode yang digunakan adalah dengan mengunakan Microplate reader atau platec reader pada panjang gelombang 410 nm untuk menentukan absorbansi, sehingga dengan analisis data diperoleh nilai Inhibitor Concentration 50% (IC50) dari ekstrak sampel dalam menghambat kerja enzim α-glukosidase dalam menghidrolisis substrat p-nitrofenil-α-D-glukopiranosida (p-NPG) untuk membentuk glukosa. Hasil dari penelitian diperoleh nilai IC50 adalah sebesar 387,6091 µg/mL. Jika dibandingkan dengan nilai IC50 untuk akarbose sebagai kontrol posistif sebesar 0,8135 µg/mL, memang masih lemah karena akarbose merupakan senyawa murni sedangkan Fraksi Metanol masih berupa ekstrak Kasar walaupun sudah di fraksinasi namun fraksi Metanol kulit jering dapat dikatakan memiliki potensi untuk dilakukan isolasi lebih lanjut sebagai eksplorasi senyawa aktif antidiabetes dalam kulit jering. Kata Kunci : Jering; Metanol; Antidiabetes; IC50.
ANTIOXIDANT ACTIVITY OF THE METHANOL EXTRACT OF TURKEY EGGPLANT (Solanum betaceum Cav.) INVITRO Surya, Alfin; Anggi Wulandari; Hesti Marliza; Zaiyar
JURNAL KATALISATOR Vol. 9 No. 1 (2024): Jurnal Katalisator Volume 9 No.1, April 2024
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v9i1.2871

Abstract

Duch Eggplant rich in phytochemicals, such as polyphenols, flavonoids, and carotenoids. Studies have demonstrated the powerful antioxidant activity of these compounds, which can shield body cells from free radical-induced oxidative damage.The aim of this study is to determine the IC50 value of the antioxidant activity present in the skin of the Holland turnip. This study also has implications for human health. Knowing the anti-oxidant skin activity in the inviter allows us to estimate the potential benefits of protecting the body from oxidative damage. 1.1-diphenyl-2-picrylhydrazil (DPPH) is the method employed. This method involves measuring the ability of the extract to neutralize the free radicals in DPPH. We will observe the color changes from purple to yellow and evaluate the free radical's capture capacity based on the degree of color change. The study's findings revealed an IC50 value of 41,019 ppm. The conclusion from the IC50 values obtained is that the methanol extract in the skin of the Dutch teranga has an antioxidant activity that is very strong to counter free radicals. 
ADSORPTION STUDY OF LEAD (Pb²⁺) IONS USING CASSAVA PEEL BIOCHAR: EFFECT OF pH AND CONTACT TIME Elfia, Mega; Etriyanto Arman; Eduwin Saputra; Alfin Surya; Yoravika Dwiwibangga; Vicka Andini; Aidil Onasis; Nadya Br Debora
JURNAL KATALISATOR Vol. 10 No. 2 (2025): Jurnal Katalisator, Volume 10 No 2 Oktober 2025
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v10i2.3484

Abstract

This study evaluates the adsorption performance of NaOH-activated cassava peel biochar (Manihot esculenta Crantz) for the removal of Pb²⁺ ions from aqueous solutions, with particular focus on the effects of solution pH and contact time. Cassava peel biochar was produced via pyrolysis at 180–350 °C and chemically activated using 0.1 M NaOH. Surface functional groups and morphology were characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy–Energy Dispersive X-ray (SEM–EDX). Batch adsorption experiments were conducted by varying solution pH (3–8) and contact time (30–120 min), while Pb²⁺ concentrations before and after adsorption were analyzed using Atomic Absorption Spectroscopy (AAS). The results showed that solution pH significantly affected Pb²⁺ adsorption, with the highest adsorption capacity of 9.94 mg/g achieved at pH 5. Contact time also influenced adsorption performance, and the optimum condition was obtained at 90 min, yielding a maximum adsorption capacity of 9.94 mg/g. These findings indicate that NaOH-activated cassava peel biochar exhibits stable and effective Pb²⁺ adsorption under slightly acidic conditions and moderate contact time, highlighting its potential as a low-cost and environmentally friendly adsorbent for Pb²⁺ removal from contaminated wastewater.