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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding BERITA BIOLOGI Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana Biota Floribunda BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
AGUS HERY SUSANTO
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Penggunaan Marka RAPD Sebagai Penduga untuk Membedakan Jenis Kelamin pada Kantung Semar Nepenthes Gymnamphora Koleksi Kebun Raya Baturraden Dini Rizki Pertiwi; Murni Dwiati; Agus Hery Susanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 1 No 2 (2019): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.144 KB) | DOI: 10.20884/1.bioe.2019.1.2.1778

Abstract

Nepenthes gymnamphora is an endemic pitcher plant species in Java Island and one of the plant collections of Baturraden Botanical Garden. N. gymnamphora is a dioecious plant and its sex cannot be differed in vegetative development. Conservation efforts can be carried out more efficiently when sex identification is performed earlier thus leading to effective development of particular sexes. Random Amplified Polymorphic DNA (RAPD) technique is one molecular approach that can be employed in early identification of N. gymnamphora sex. The aims of this study are to assess whether there is difference of RAPD patterns between male and female N. gymnamphora and to find out how the difference is. Explorative method was applied to this study involving five N. gymnamphora individuals of Baturaden Botanical Garden collection of different sexes as samples. Genomic DNAs were extracted from youngest leaves of the five samples (two males, two females and one individual of unknown sex) and then used as templates to amplify RAPD markers. Five random primers were used in the amplification, i.e. OPK-16, OPP-15, OPA-15, OPP-08, and OPD-20. Two primers, i.e. OPP-08 (5’-ACATCGCCA-3’) and OPD-20 (5’-ACTTCGGCCAC-3’), produced RAPD bands of approximately 300 bp in males and sexually unknown individual. These bands did not appear in females, so that it can be presumably related to sex determination genes in N. gymnamphora. Primer OPP-08 also produced RAPD bands of approximately 250 bp in females individual. These bands did not appear in males, so it can be presumably related to sex determination genes in N. gymnamphora.
Shoot Tip Culture: A Potential In Vitro Culture Multiplication of Screw Pines (Pandanus tectorius Park.) Ikhsan - Matondang; Sisunandar .; Alice Yuniaty; Triani Hardiyati; Agus Hery Susanto; Endang Srimurni Kusmintarsih
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4208

Abstract

Pandanus tectorius Park., tree of life, which almost all of its parts are used by humans. Industrial fiber made from screw pine leaves has been successfully exported to several countries, while the fruit is consumed as an alternative staple food. Fruit is only produced by female plants, because screw pine is dioecious plant. Farmers obtain screw pine seedling using stem cuttings from female trees, but the number of seedling produced is limited and this technique can damage the plant. Alternative seedling production using seeds has constraints in the form of uncertainty over the sex of the seedling. One of the best ways to provide screw pine seedling is to use a shoot-tip culture technique. Shoot-tip culture is able to reproduce plants efficiently and quickly and does not damage the plant. Seedling will also have the same genetic traits as the selected plant. However, efforts to develop an efficient and fast screw pine shoot-tip culture protocol have not been carried out massively. The induction and shoot multiplication stages still had a low success rate. Another problem in seedling production through tissue culture is the certainty seedlings are true-to-type. This review article aims to reveal the potential of shoot-tip culture for in vitro screw pine seedling production and to test the genetic stability of the screw pine seedling.  
VARIATION IN SUGAR CONTENT AND DISTRIBUTION IN SYZYGIUM SAMARANGENSE FRUITS Pudji Widodo; Elly Proklamasiningsih; Murni Dwiati; Agus Hery Susanto
Floribunda Vol. 7 No. 3 (2023): Floribunda Oktober 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i3.2023.422

Abstract

Jambu semarang (Syzygium samarangense) terdiri dari banyak kultivar dengan kadar gula yang berbeda-beda. Pada setiap buah, kandungan gulanya tidak merata. Tingkat kemanisan buah seringkali menjadi penentu nilai ekonomi dari jambu semarang. Tujuan penelitian ini adalah 1) untuk mengetahui sebaran gula pada berbagai kultivar jambu semarang, 2) mengetahui sebaran kadar gula di dalam buahnya, 3) mengetahui pengaruh rerata hari hujan per bulan terhadap kandungan gula buah.  Metode yang digunakan dalam penelitian ini adalah survei dengan purposive sampling, observasi laboratorium dengan mengukur kadar gula dengan refraktometer, mengkorelasikan faktor lingkungan dengan kandungan gula buah. Hasil penelitian ini menunjukkan bahwa buah dengan kadar gula tertinggi terdapat pada kultivar Syzygium samarangense 'Sukaluyu' (maks 16,5 ◦Brix), S. samarangense 'Madu' (maks 14 ◦Brix), S. samarangense 'Kesuma Merah’ (max 10.5 ◦Brix), S. samarangense 'Citra' (maks 8.5◦Brix) yang cocok untuk buah-buahan segar. Sedangkan yang kadar gulanya rendah seperti S. samarangense 'Bajang Leang’ (4.5 ◦Brix), S. samarangense 'Kuning’ (4 ◦Brix) lebih cocok untuk lutis. Pada buah S. samarangense, gula tertinggi biasanya terakumulasi di bagian ujung buah, dan gula terendah berada di pangkal buah.  Kadar gula berkorelasi negatif dengan rerata hari hujan per bulan.  Makin sedikit hari hujan, buah semakin manis.  
Morphological and Genetic Diversity of Mangrove Species Ceriops tagal (Perr.) C.B. Rob. Around Java Island Sukmarani, Dhuta; Proklamasiningsih, Elly; Susanto, Agus Hery; Ardli, Erwin Riyanto; Permadi, Jefri; Palimirmo, Flandrianto Sih
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 4 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.4.334-350

Abstract

Ceriops tagal (Perr.) C.B. Rob can be found in Baluran National Park, Kepulauan Seribu National Park, Karimunjawa National Park, and Segara Anakan Cilacap in Java and its surrounding areas. These locations have different demographics and environmental conditions. Plant species found in different geographical areas have varied genetic structures and morphological traits as a result of their adaptability to their environment. The goal of this study is to evaluate the morphological and genetic differences between the four populations of C. tagal in Java and its surroundings. Internode length, leaf length, leaf width, petiole length, and leaf area were the morphological characters used. The Internal Transcribed Spacer (ITS) region is used as a molecular marker. The UPGMA dendogram was used to analyze the morphological similarity, and multidimensional unfolding analysis was used to determine which character most determined the grouping. Mega X software was used to analyze genetic variation using the neighbor-joining approach, while Arlequin 3.5 software was used to estimate genetic variation within and among populations. The results of this study showed that the population of Baluran National Park has features or is distinct from the other three populations based on morphological and genetic characters; this could be because of variations in geographic conditions, and that population diversification was aided by a combination of genetic and physical divergence, restricted gene flow, and local adaptation.
Relative Expression of mRNA BARF1 Epstein-Barr Virus from Tumor Tissue Biopsy in Formalin-Fixed Paraffin-Embedded in Nasopharyngeal Carcinoma Wahyono, Daniel Joko; Fuadi, Muflih; Susanto, Agus Hery; Sulistyo, Hidayat
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.413

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from nasopharyngeal epithelial cells. NPC is endemic in some regions, especially in Southeast Asia countries. NPC is also a multifactorial disease involving environmental factors, genetic factors, and infection from Epstein-Barr virus (EBV). According to WHO classification, Undifferentiated NPC is histopathologically associated with EBV infection and categorized into WHO subtype 3. EBV has two phases in its infection cycle: the lytic and latent phases. The BARF1 gene is a mediator of the transition from the latent phase to the lytic phase. Previous studies suggest measurement of EBV mRNA activity at the primary tumor site in the nasopharyngeal reflects the pathogenesis of NPC rather than measuring circulating EBV DNA or serological diagnosis. This study aimed to determine the relative expression potential of BARF1 mRNA at different tumor stages in NPC patients as a predictor of NPC pathogenesis. This research design was a descriptive research method in the form of a cross-sectional study. The samples used were 22 patients diagnosed as NPC WHO class III at the Anatomical Pathology Section of Prof. Dr. Margono Soekarjo, Purwokerto, who met the inclusion criteria. The relative expression of BARF1 mRNA was carried out using the one-step real-time RT-PCR technique and then calculated using a formula of 2-ΔCt. The T-test was used to compare the relative expression of early and late-stage BARF1 mRNA. The relative expression of BARF1 mRNA in the late-stage advanced stage (n = 6; 0.708292-0.840177; med = 0.7164655) was increased compared to the early stage (n = 2; 0.708841-0.712423; med = 0. 710632).
Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment Dwiati, Murni; Hasam, Wahyu Nur; Susanto, Agus Hery
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.197

Abstract

Background: Vanda limbata is a natural orchid species found on Java Island, commonly known as V. limbata 'Jawa.' Enhancing plant vigor is essential to increase its potential as an ornamental plant, with one promising approach being induced polyploidy using chemical mutagens such as oryzalin. Objective: This study aimed to evaluate the effect of oryzalin on inducing polyploidy in V. limbata cultured in vitro. Method: Oryzalin was applied at concentrations of 0, 25, 50, 75, and 100 µM, with five replications for each treatment, resulting in 25 experimental units. Several morpho-physiological and anatomical traits were measured as indicators of polyploidy. Results: The results demonstrated that oryzalin at 100 µM was the most effective concentration for inducing polyploidy in V. limbata cultured in vitro. This was particularly evident in traits such as reduced leaf length, increased leaf width, enhanced adventitious shoot formation, and enlarged stomatal width. Conclusion: Oryzalin, when applied at appropriate concentrations, can be effectively used to induce polyploidy in V. limbate.
Some Morphological and Physiological Characters of Vanda bensonii Bateman Seedlings under Colchicine Application Khairunissa, Furidian; Dwiati, Murni; Susanto, Agus Hery
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.17191

Abstract

ABSTRACT. Vanda bensonii is an orchid species of some potentials to develop, one of which is by means of induced mutation resulting in polyploid individuals. Such individuals usually exhibit better phenotypical performance than that of the normal diploids. This can be performed by the application of antimitotic chemicals such as colchicine. This study aims to know the effect of colchicine on some morphological and physiological characters of V. bensonii seedlings and to find out the effective concentration in producing the most promising ideal phenotypical performance of the orchid species. The study was conducted at the Plant Physiology Laboratory, the Faculty of Biology, Universitas Jenderal Soedirman by using an experimental method arranged in a Completely Randomized Design with treatments consisting of five colchicine concentrations, i.e. 0, 20, 40, 60, and 80 µM. Each treatment was subjected to five replications giving rise to a total of 25 experimental units. The data obtained were analyzed using ANOVA with an F test of 0.05 and 0.01. Further analysis using Tukey test of 0.05 was performed when significant differences among treatments were found. The results show that colchicine significantly reduces plant height of V. bensonii, in which control plant shows taller in comparison to all treatments. Similarly, it is also the case with chlorophyll a content, while there is no significant effect of colchicine on leaf width, leaf length, leaf number, shoot number, chlorophyll b and total chlorophyll contents. Nevertheless, colchicine concentration of 60 µM is found most effective in increasing shoot number. It can be concluded that colchicine has significant effects on chlorophyll a content of V. bensonii, implying its potential application to create mutants of better performance. Keywords: colchicine, morphological characters, physiological characters, polyploid, Vanda bensonii
GENETIC VARIABILITY OF POLYMESODA EROSA POPULATION IN THE SEGARA ANAKAN CILACAP Nuryanto, Agus; SUSANTO, AGUS HERY
BIOTROPIA Vol. 17 No. 1 (2010): BIOTROPIA Vol. 17 No. 1 June 2010
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1085.718 KB) | DOI: 10.11598/btb.2010.17.1.37

Abstract

Abstract Mud clams Polymesoda erosa in the Segara Anakan Cilacap are highly exploited by the local communities for daily consumption. This is presumed causing population decline and potentially causing loss of genetic diversity. Genetic diversity level within population can be obtained by population genetic study using molecular marker such as randomly amplified polymorphic DNA (RAPD). Here we amplified RAPD marker using ten arbitrary primers to assess genetic diversity of P. erosa population in the Segara Anakan Cilacap to provide genetic data for its sustainable use. The result proved that the used RAPD marker has high polymorphisms. The mud clam population was also showed a high level of heterozigosity and genetic diversity.  This has important implication for the management plan towards ustainable use of P. erosa in the Segara Anakan Cilacap.   Key words: mud clam, RAPD, polymorphism, genetic diversity
Polymorphic Profiles of Ganoderma Spp. Isolates From Banyumas, Central Java, Indonesia Ratnaningtyas, Nuniek Ina; Susanto, Agus Hery; Yulia, Ana
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.2.884

Abstract

Ganoderma spp. are known as both beneficial and harmful fungi to humans. These are distributed worldwide in sufficiently high diversity. To generate a polymorphic profile and a genetic inter-relationship of several isolates of Ganoderma spp., a study was conducted using the Random Amplified Polymorphic DNA (RAPD) markers on Ganoderma spp. from Banyumas Regency, Central Java, Indonesia. The fruiting bodies of the collected Ganoderma spp. were first morphologically characterized, then analyzed using RAPD with four random primers, i.e., OPC-1, OPC-2, OPC-4, and OPC-5. The results revealed that the four primers generated polymorphic bands of the 10 samples with a polymorphism level of 100%, showing high genetic diversity. The level of genetic similarity ranged between 0.48 and 0.82, indicating moderate similarities among samples. The constructed dendrogram resulted in the grouping of the Ganoderma spp. isolates into three clusters at a similarity coefficient of 0.63, but neither according to geographical locations nor growth substrates.
Co-Authors A.A. Ketut Agung Cahyawan W Adi Amurwanto Agus Nuryanto Alice Yuniaty Ardli, Erwin Riyanto BUDI SETIADI DARYONO Daniel Joko Wahyono Dhuta Sukmarani, Dhuta Dini Rizki Pertiwi ELLY PROKLAMASININGSIH Elly Proklamasiningsih ENDANG SRIMURNI KUSMINTARSIH Flandrianto Sih Palimirmo, Flandrianto Sih Fuadi, Muflih Hasam, Wahyu Nur Hendro Pramono Hendro Pramono Hidayat Sulistyo Ikhsan - Matondang Jefri Permadi Khairunissa, Furidian Murni Dwiati Murni Dwiati Murni Dwiati Nuniek Ina Ratnaningtyas Nurtjahjo Dwi Sasongko Petrus Hary Tjahja Soedibja Pudji Widodo Saefuddin Aziz SATRIYAS ILYAS Sisunandar . Suhestri Suryaningsih Suryadi Suryadi Suryaningsih, Suhestri triani hardiyati Yulia, Ana