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Pendugaan Nilai Heritabilitas Sorgum (Sorghum bicolor (L.) Moench) Lokal Jember Hasil Mutasi Sinar Gamma Akbar Rafsanjani; Iskandar Umarie; Bejo Suroso; Hidayah Murtiyaningsih; Laras Sekar Arum
National Multidisciplinary Sciences Vol. 2 No. 3 (2023): Proceeding SEMARTANI 2
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/nms.v2i3.302

Abstract

Penelitian ini bertujuan untuk (1) mengetahui tingkat keragaman genetik dan fenotip dari sorgum varietas lokal hasil mutasi sinar gamma. (2) Untuk mengetahui tingkat heritabilitas dari sorgum varietas lokal hasil mutasi sinar gamma. Dalam penelitian ini menggunakan rancangan acak kelompok (RAK) faktor tunggal yaitu genotipe sorgum lokal Jember hasil mutasi sinar gamma Faktor kedua yaitu dosis iradiasi sinar gamma. Hasil penelitian menunjukan nilai (KKG) dan (KKF) dari karakter agronomis tinggi tanaman, diameter batang, jumlah anakan, Panjang penikel, jumlah biji, berat total biji dan berat 1000 biji memiliki nilai yang tinggi, sedangkan umur berbunga dan jumlah daun memiliki nilai yang rendah. Karakter komponen hasil yang memiliki kriteria heritabilitas tinggi adalah tinggi tanaman, jumlah daun, diameter batang, umur berbunga, panjang penikel, berat 1000 biji, jumlah anakan dan jumlah biji sedangkan karakter berat total biji memiliki kriteria heritabilitas sedang. karakter yang memiliki nilai KKG dan heritabilitas tinggi lebih besar dipengaruhi oleh faktor genetik. Dengan demikian, penampilan fenotipe karakter tersebut akan terekspresi sebagai pengaruh genetik dan sedikit dipengaruhi lingkungan.
Penguatan Pangan Lokal Berkelanjutan Melalui Edukasi Dapur Sehat Atasi Stunting Bagi Keluarga Berisiko Binaridha Kusuma Ningtyas; Hidayah Murtiyaningsih; Laras Sekar Arum
Jurnal Pengabdian Teknologi Informasi dan Kesehatan (DIANKES) Vol. 1 No. 2 (2023): June
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/diankes.v1i2.12

Abstract

Stunting has become a severe problem and has become a global issue. The condition of stunting is malnutrition which can hamper the physical development and intelligence of children, so that it will impact the quality of life in the future. Resolving stunting takes a very long time. It requires changes in behaviour, mindset, and strong political commitment from stakeholders. Understanding stunting is not only a health issue, but also covers a broader aspect. Stunting is a multidimensional issue closely related to social, economic, and educational inequality. The government has carried out many programs or policies to deal with stunting, significantly strengthening assistants or cadres at the village level. This effort aims to provide education and assistance in handling stunting, especially for prospective brides, pregnant women, nursing mothers, and toddlers. In addition to strengthening assistants in the field, the community can support the Dapur Sehat Atasi Stunting (DASHAT) institution. DASHAT is a community participation-based program to encourage balanced nutrition for families at risk of stunting by strengthening local food sources. Community service activities occur in Suci Village, Panti District, Jember Regency. Community service activities are carried out with DASHAT education and assistance by encouraging the strengthening of local food for families at risk of stunting. This activity uses a people-driven centre approach. Through this approach, participants identify and develop local food-based menus that can be used to fulfil balanced nutrition to overcome stunting. This activity can explain to at-risk families that if appropriately processed, local food sources can produce nutritious food.
Pengaruh ZPT Alami dan Komposisi Media Terhadap Pertumbuhan Stek Puring (Codiaeum variegatum) Bagus Tripama; Ahmad Rizki Junaedi; Hidayah Murtiyaningsih; Bejo Suroso; Laras Sekar Arum
BIOSAPPHIRE: Jurnal Biologi dan Diversitas Vol. 1 No. 2 (2022): BIOSAPPHIRE
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, UNIPAR JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biosapphire.v1i2.699

Abstract

Penelitian ini bertujuan untuk mengetahui respon pertumbuhan stek tanaman puring pada beberapa zat pengatur tumbuh alami dan macam komposisi media. Rancangan yang digunakan RAK faktorial dengan 2 faktor dan 3 kali ulangan meliputi : faktor pertama ZPT alami (T) yaitu T0 (tanpa ZPT), T1 (ekstrak daun kelor 30%), T2 (ekstrak taoge kacang hijau 60%), dan T3 (ekstrak bawang merah 75%), sedangkan faktor kedua komposisi media (M) terdiri dari M0 (tanah), M1 (tanah + arang sekam 1 : 1), M2 (tanah + cocopeat 1 : 1), M3 (tanah + kompos daun bambu 1 : 1). Hasil analisis ragam menunjukkan bahwa perlakuan T berpengaruh sangat nyata terhadap parameter persentase stek bertunas berakar, total persentase stek bertunas tak berakar, panjang tunas 4, 6 dan 8 mst, panjang akar, jumlah akar dan volume akar. Serta berpengaruh nyata terhadap jumlah tunas 6 mst, sedangkan terhadap persentase stek hidup, persentase stek bertunas, dan jumlah tunas 4 dan 8 mst berpengaruh tidak nyata. Perlakuan M berpengaruh sangat nyata terhadap parameter persentase stek bertunas berakar dan tak berakar, jumlah akar, dan volume akar, serta berpengaruh nyata terhadap panjang tunas umur 4 mst, jumlah tunas 6 dan 8 mst dan panjang akar, sedangkan terhadap persentase stek hidup, persentase stek bertunas, panjang tunas 6 dan 8 mst, dan jumlah tunas 4 mst berpengaruh tidak nyata. Adapun interaksi antara T×M berbeda tidak nyata terhadap semua parameter pengamatan, kecuali persentase stek bertunas berakar dan tak berakar berbeda sangat nyata. Secara keseluruhan zat pengatur tumbuh alami ekstrak daun kelor (T1) dan komposisi media tanah + arang sekam (M1) menunjukkan yang terbaik
Pemberdayaan UMKM Melalui Pembuatan Gula Cair dari Limbah Kulit Singkong sebagai Pemanis Alternatif Danang Kumara Hadi; Murtiyaningsih, Hidayah; Mega Saniya Juli Nuriyatul; Ega Dwi Sukmadiningsih
PEKAT: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 1 (2024): April
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/pekat.v3i1.33

Abstract

Kabupaten Jember, memiliki lahan pertanian komoditi singkong yang luas, memberikan peluang besar untuk mengembangkan produksi alternatif. Potensi pengembangan alternatif pemanis dari limbah kulit singkong melalui Program Kemitraan Masyarakat Stimulus (PKMS) di Kabupaten Jember, Jawa Timur. Gula cair yang dihasilkan dari kulit singkong menawarkan nilai ekonomis yang menjanjikan, dengan 1 liter gula cair dihasilkan dari setiap 15 kg bahan baku. Melalui pelatihan dan pendampingan, PKMS bertujuan untuk meningkatkan keterampilan dan produksi mitra pengusaha produk olahan singkong, mengajarkan ekstraksi pati dan pembuatan gula cair rendah kalori. Sasaran kegiatan melibatkan UMKM produk bahan olahan singkong di Jember, dengan harapan hasilnya dapat bersaing dengan gula impor dan mengurangi laju impor gula. Hasil keberdayaan mitra mencakup peningkatan keterampilan dan pengetahuan dalam produksi gula cair dari kulit singkong. Mitra dapat meningkatkan keterampilan dalam pengolahan limbah kulit singkong menjadi gula cair rendah kalori. Diharapkan mitra dapat meningkatkan efisiensi limbah kulit singkong dan menghasilkan produk yang lebih berkualitas. Selain itu, keberdayaan mitra juga dapat tercermin dalam kemampuan mereka untuk bersaing di pasar lokal dan internasional, dengan dampak positif pada pengurangan laju impor gula di tingkat lokal. Kata Kunci: Gula cair, Limbah kulit singkong, UMKM  
IDENTIFIKASI KERAGAMAN GENETIK SORGUM LOKAL HASIL IRADIASI MENGGUNAKAN PENANDA MOLEKULER RPAD Arum, Laras Sekar; Ramadhan, Moch. Alfian Rizky; Hazmi, Muhammad; Murtiyaningsih, Hidayah
Jurnal Penelitian Pertanian Terapan Vol 24 No 1 (2024)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v24i1.3265

Abstract

Sorghum (Sorghum bicolor L. Moench) is an alternative food commodity with significant potential for development in Indonesia. However, its genetic diversity in Indonesia is relatively low. The low genetic diversity of sorghum prompts efforts to improve and search for new sources of genetic diversity, including through plant breeding. Radiation-induced mutation is a plant breeding technique aimed at obtaining new traits that are not present in the parent plants. Gamma ray irradiation can damage DNA, and during the repair process, DNA undergoes new mutations induced randomly. This study aims to investigate the agronomic characteristics, genetic diversity, and relationship among plants that have been irradiated with gamma rays using the Random Amplified Polymorphic DNA (RAPD) molecular markers. In this study, local sorghum varieties from Bandung, Demak, and Jember  were subjected to gamma ray irradiation at doses of 0 Gy, 300 Gy, and 500 Gy to induce genetic diversity. The application of gamma ray irradiation doses had a significant impact on parameters such as viability, vigor, and morphology. The isolated DNA was analyzed using the Random Amplified Polymorphic DNA technique. OPA-12 showed the highest level of polymorphism among the four primers used. The bands produced by the four scored primers were analyzed using the NTSYSpc program to determine the level of relatedness between genotypes. The genetic distance between genotypes was considerably wide, ranging from 0.1 to 0.46, indicating the potential for crossbreeding
Efektivitas Konsentrasi Giberelin (Ga3) Dan Waktu Penyiangan Terhadap Pertumbuhan Dan Hasil Tanaman Mentimun (Cucumis Sativus L.) Rima, Baidhatul Khoirimah; Oktarina, Oktarina; Murtiyaningsih, Hidayah
Callus: Journal of Agrotechnology Science Vol. 2 No. 1 (2024): Maret
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/callus.v2i1.2072

Abstract

Jumlah penduduk Indonesia terus bertambah dan konsumsi mentimun juga semakin meningkat. Efektivitas Konsentrasi Giberelin (GA3) dan Waktu Penyiangan Terhadap Pertumbuhan dan Hasil Tanaman Mentimun (Cucumis Sativus L.). 2 faktor dalam penelitian ini disusun dalam Rancangan Acak Kelompok (RAK) faktorial dalam 3 ulangan. Faktor yang pertama konsentrasi giberelin G0 (0 ppm/kontrol) G1 (50 ppm) G2 (150 ppm) G3 (200 ppm), dan faktor yang kedua penyiangan P0 (tanpa penyiangan) P1 (disiangi 1&3 mst) P2 (disiangi 2&4 mst). Konsentrasi giberelin berpengaruh secara nyata terhadap semua parameter pengamatan kecuali panjang tanaman mentimun 7 hst dan 14 hst, jumlah daun mentimun 28 hst dan jumlah buah mentimun. Perlakuan terbaik ada pada G3 (200 ppm). Penyiangan berpengaruh secara nyata terhadap semua parameter pengamatan kecuali panjang tanaman mentimun 28 hst, jumlah daun mentimun 21 hst, jumlah buah mentimun dan berat mentimun per buah. Perlakuan terbaik diperoleh pada perlakuan P2 (disiangi 2&4 mst). Interaksi antara konsentrasi giberelin dan penyiangan berbeda secara nyata terhadap semua parameter kecuali panjang tanaman mentimun 7 hst dan 21 hst dan berat mentimun per buah.
Multiplication Of Pisang “Agung” (Musa Paradisiaca L.) With In Vitro As A Local Product For Food Security Fatechan, Billy; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.630

Abstract

Pisang “Agung”(Musa paradisiaca L.) is one of the banana varieties originating from Lumajang Regency. Pisang Agung is famous for its large fruit and sweet taste. The conventional way of propagating Pisang “Agung” takes a long time, produces few shoots, is not uniform and is not guaranteed to be disease-free, these obstacles can be overcome by using tissue culture. The design that will be used in this study is a Non-Factorial Completely Randomized Design (CRD) consisting of 100 ppm BAP (B) which consists of 4 levels, namely B0 (0 ppm), B1 (3 ppm), B2 (6 ppm), B3 (9 ppm). The parameters observed were the age of shoot emergence, shoot height, number of shoots. The results showed that the treatment of various concentrations of BAP gave a significant effect on all observation parameters with the best treatment B3 age of bud emergence (9.25 days), bud height is B3 (7.35 cm) and the number of buds is B0 (3.90 pieces)
Genetic Diversity Test of Various Javanese Local Sorghum Us-ing RAPD Molecular Markers Utami, Erisa Putri; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.633

Abstract

Sorghum is one of the cereal crops that has good growth adaptation and production on dry land so that it has the potential to be developed in Indonesia. Besides being able to produce in dry areas, sorghum has a higher protein content compared to corn and cracked rice, but lower than wheat. Local sorghum used in this study included local Jember, local Demak, local Nganjuk, and local Majalengka. The level of genetic diversity of sorghum in Indonesia is still relatively low. Therefore, it is necessary to test the genetic diversity of sorghum with plant breeding. To obtain genetic diversity, this research conducted identification using RAPD molecular markers with 9 primers, namely OPA- 01, OPA-02, OPA-03, OPA-04, OPA-05, OPA- 06, OPA-11, OPA 17, and OPA 18. Based on the results of RAPD, the results of DNA bands were obtained which were continued with the NTSYSpc program to produce a kinship tree between local sorghum plants. From the results of the phylogenetic tree, it is known that the local sorghum of Majalengka and Lamongan have a parallel kinship, which means that the sorghum is genetically the same. Meanwhile, the genetic diversity of local sorghum in Jember and Demak is far compared to Lamongan and Majalengka. Nganjuk local sorghum has a genetic diversity line position in the middle (Jember, Demak) and (Majalengka, Nganjuk).
Screening of Auxin-Producing Bacteria from Several Crops Rhizospheres Prasetyo, Vandi Putranto; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.634

Abstract

Plants have natural compounds that can stimulate plant growth and development called phytohormones. Phytohormones can be produced endogenously by plants themselves and are not optimal. Therefore it requires exogenous phytohormones that come from outside the plant. In some cases, non-pathogenic bacteria can promote plant growth and be used in agriculture as biofertilizers. Soil rhizospheres from crops are suspected that the soil in the root area (rhizosphere) contains bacteria that stimulate plant growth. Hormones that play a role in plant growth function in division, enlargement of sprout cells, and division of cells at the root growth point. This research aims to determine bacteria that potentially produce the hormone auxin. This research identifies bacterial isolates from microscopic and macroscopic characterization. Screening isolates of auxin-producing bacteria using colorimetric methods and spectrophotometric methods. The results of characterization and screening from 5 samples showed that the bacterial samples that had the potential to produce auxin were samples isolated from eggplant soil (e) with a concentration of 6.5 ppm. The auxin produced is auxin IBA (Indole-3-butyric acid). The IBA auxin content is high enough to be applied as a plant growth-promoting bacteria.
Efforts to Propagate Sorghum In Vitro to Support Local Food Aisyah, Siti; Hazmi, Muhammad; Murtiyaningsih, Hidayah
International Applied Science Vol. 3 No. 2 (2024): Proceeding of International Conference of Agriculture (Semartani-3)
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/ias.v3i2.635

Abstract

indonesia is a country known for its abundant biodiversity. however, the majority of its people still make rice as a daily staple food, making the price of rice continue to increase until it is difficult to reach by low-income people. therefore, it is necessary to develop alternative local food to replace rice to avoid a food crisis. One of the alternative plants is sorghum. Because sorghum has many benefits besides as food as well as bioenergy. That way it is necessary to make efforts to develop sorghum, one of which is by means of embryogenic callus tissue culture techniques by adding a concentration of 2,4-D. This research was conducted in the Laboratory of Nutraseutical and Pharmaseutical Division of Center for Development of Advancd Science and Technologies (CDAST) UPA Waste Management and Integrated Laboratory, University of Muhammadiyah Jember by using Completely Randomized Design (CRD) with a single factor namely A0 (control), A1 1 ppm, A2 2ppm, A3 3ppm, A4 4 ppm which was repeated 3 times. The variables under observation are the callus's color, texture, percentage, and time of formation. According to the study's findings, the application of 4 ppm 2,4-D produced the best results when compared to other 2,4-D treatments. These treatments were able to produce callus at a rate of 4.11 his, 100% of the total number of callus, crumbly texture, and pale yellowish color.