Article Per Year (5 Year)
p-Index From 2021 - 2026
2.59
P-Index
This Author published in this journals
All Journal ACTA VETERINARIA INDONESIANA BALI MEDICAL JOURNAL (BMJ) Medicina Buletin Udayana Mengabdi E-Jurnal Medika Udayana Jurnal Veteriner Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Syntax Literate: Jurnal Ilmiah Indonesia ISM (Intisari Sains Medis) : Jurnal Kedokteran Indonesian Community Empowerment Journal Jurnal Ilmu Multidisplin
I Kadek Swastika
Departemen Parasitologi, Fakultas Kedokteran Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

Published : 44 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Jurnal Veteriner

 1 
Protein Spesifik Cairan Kista Cysticercus bovis pada Sapi Bali yang Diinfeksi dengan Taenia saginata (SPECIFIC PROTEIN OF CYSTICERCUS BOVIS CYST FLUID ON BALI CATTLE EXPERIMENTALLY INFECTED WITH TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; Kadek Swastika; I Made Damriyasa; Ida Bagus Made Oka; I Nyoman Mantik Astawa
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.214 KB)

Abstract

Cysticercus bovis is the larval stage of Taenia saginata, the bovine tapeworm. The infection of thislarval in cattle musculature causes Bovine cysticercosis or Cysticercosis bovis.  Bovine cysticercosis is foundworldwide, but mostly in developing countries, where unhygienic conditions, poor cattle managementpractices, and the absence of meat inspection are common.  The adult Taenia infection in man is referredto as taeniasis.  Taenia saginata taeniasis is also found almost all over the world.  The prevalence ofTaenia saginata taeniasis has reported up to 27.5% in Gianyar Bali. In order to control the diseases,vaccination against the larvae stages in cattle of Taenia saginata may play an important role in controllingthe disease in the endemic regions.  The aims of the present study were to prepare and to investigate theimmunogenic protein as vaccine candidate for controlling  Cysticercus bovis infection in in Bali cattle.Cysticercus protein from the cyst fluid was firstly used to immunize mice and the mice sera were thencollected. Cysticercus proteins then analyzed using sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE).All cysticercus proteins were then visualized by Commasie blue staining. The proteins were also transferredonto nitrocellulose membrane and the immunogenic proteins were visualized by Western Blotting usingimmune sera raised in mice.  By Commasie blue staining, a total of 17 proteins were detected with themolecular weight of 14,86 kDa -122,40 kDa from the smallest to the largest. As many as 7 immunogenicproteins with the molecular weights of 16.81 kDa; 19.22 kDa; 20.98 kDa; 27.41 kDa; 34.02 kDa; 38.31 kDa;and 54.94kDa were detected.
Mosquito-specific viruses (family Flaviviridae, genus Flavivirus) Diisolasi pada Nyamuk Anopheles vagus di Bali Putu Ayu Asri Damayanti; I Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi; I Made Sudarmaja; I Kadek Swastika; Dewa Ayu Agus Sri Laksemi; Ni Luh Putu Eka Diarthini
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.933 KB) | DOI: 10.19087/jveteriner.2021.22.2.189

Abstract

Mosquito-specific viruses (MSVs) adalah virus yang hanya dapat bereplikasi pada sel nyamuk. Virus ini terdiri dari berbagai genus, salah satunya yang paling banyak ditemukan adalah dari famili Flaviviridae, genus Flavivirus. Namun, data keberadaan dan karakteristik MSVs dan vektornya di Bali saat ini sangat terbatas. Oleh karena itu, pengamatan untuk memperluas penemuan keragaman vektor dan filogenetik MSVs famili Flaviviridae, genus Flavivirus di Bali dilakukan pada tahun 2016-2018. Nyamuk dewasa ditangkap menggunakan light trap dan dikelompokkan berdasarkan spesies. Isolasi dan propagasi virus dilakukan pada galur sel C6/36 dan baby hamster kidney-21 (BHK-21). Identifikasi virus dilakukan dengan menggunakan one step reverse-transcriptase polymerase chain reaction (RT-PCR). Terdapat dua pool yang berasal dari nyamuk Anopheles vagus menampakan cythopathic effect (CPE) hanya pada galur sel C6/36 dari total 158 pool. Virus yang diisolasi memiliki persentase identity sekuen nukleotida tertinggi 97% dan sekuen asam amino 96% dengan virus Culex theileri Flavivirus isolat JKT-8650 yang diisolasi pada tahun 1981. Selanjutnya, virus dinamakan Mosquito Flavivirus Isolate Bali (MFB) dengan accession numbers KY995166 dan KY290258. Analisis filogenetik menunjukan bahwa MFB berada satu kluster dengan Culex theileri Flavivirus (CTFV) dari Indonesia, Culex Flavivuruses-Myanmar, Culex theileri Flavivirus-Portugal, dan Mosquito Flavivirus-Turki. Terdapat delapan nukelotida dan enam asam amino yang berbeda antara MFB dan CTFV Indonesia. Pada penelitian ini dapat disimpulkan bahwa MSVs dari famili Flaviviridae, genus Flavivirus berhasil diisolasi dari nyamuk An. vagus di Bali.
Present Situation and Problems of Cysticercosis in Animal in Bali and Papua Nyoman Sadra Dharmawan; Kadek Swastika; I Made Putra; Toni Wandra; Putu Sutisna; Munehiro Okamoto; Akira Ito
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.341 KB)

Abstract

Cysticercosis zoonotic parasitic disesase caused by Taenia solium larva, is a major public healthproblem. Cysticercosis results from the development of larval tapeworms in humans harboring adult T.solium or from ingesting soil containing eggs shed in the feces of humans, in areas where there are nolatrines. Humans are accidental intermediate hosts and pigs are the normal intermediate hosts. Clinicallythey are most serious when located in the central nervous system or in the eye where they persist formonths to years. Cysticercosis occurs worldwide primarily in developing country where pigs are raised,pork consumed and poor sanitation allows pigs’ access to human faeces. The occurance of the diseases isexpected to increase in relation to the growing demand for pork in those countries. In Indonesia cyticercosisis still a very important health problem, especially in Bali and Papua. The majority of the populations inBali and Papua are Hindus and Protestants/Catholics, respectively, so pork widely consumed in thoseislands. This brief review summarizes the present situation and problems of cysticercosis in animal inBali and Papua. Epidemiological data of the diseases were obtained from several sources.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.976 KB)

Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Co-Authors Abdul Karim Abdulhadi FA Adiputra, I Komang Hotra Akira Ito Anak Agung Ayu Mirah Adi Ariwati, Luh Ariwati, Ni Luh Cokorda Agung Wahyu Purnamasidhi Damayanti, Putu Asri Daondy Friarsa Soeharto Dewa Ayu Agus Sri Laksmi Divya Nirmala, Putu Ayu Fitri N, Moh. Yasin Fuad Adi Rosyadi Hartanto, Erlin Veronica Hidajati, Sri I Gede Mahardika I Gusti Agung Ayu Chintya Cahyarini I Gusti Agung Dwi Putri Anjani I Gusti Kamasan Arijana I Ketut Tunas I Made Damriyasa I Made Dwinata I Made Putra I Made Sudarmaja I NYOMAN MANTIK ASTAWA I Putu Dodik Supartha Ida Ayu Dewi Wiryantini Ida Ayu Ide Larassanthi Pratiwi Ida Bagus Made Oka Ida Bagus Putra Adyatma Isyaputri, Rahmadany Ivy Cerelia Valerie Jevon Indra Susanto Jiriadana, Teenu Chandra Kadek Karang Agustina kumara, i made bagus bayu L. Ariwati Latifa, Kurnia Dwi Luh Ariwati Luh Dewi Anggreni Maretta Rosabella Purnamasari Maretta Rosabella Purnamasari Maria Krishnandita Munehiro Okamoto N.L Ariwati N.L.P.E. Diarthini Ni Luh Ariwati Ni Luh Ariwati Ni Luh Komang Sumi Arcani Ni Luh Putu Eka Diarthini Ni Luh Putu Eka Diarthini, Ni Luh Putu Eka Ni Made Pasmiati Setyaningsih Ni Nyoman Agustianingsih Ni Putu Akopita Devi Ni Putu Ayu Elistya Ning Purwani Ni Putu Tamara Bidari Suweta Nyoman Sadra Dharmawan P.A.A. Damayanti Pratisthita Sukadana, I Made Ananda Prajna Purantara, Anak Agung Gede Wiweka Winahya Putu Ari Paramitha Widiani Putu Ayu Asri Damayanti Putu Ayu Larasati Putu Bagus Onicha Baskaranatha Putu Sutisna Sari, Komang Ayu Kartika Shabrina Inderjit Stan ley Sukmawati Basuki Toni Wandra Upik Mutiara Wulanyani Wulanyani