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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Penelitian Hasil Hutan Jurnal Sain Peternakan Indonesia Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Hasil Hutan Jurnal Kedokteran Hewan
Diah Tri Widayati
Department Of Animal Breeding And Reproduction, Faculty Of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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RESPON ESTRUS PADA KAMBING PERANAKAN ETTAWA DENGAN BODY CONDITION SCORE 2 DAN 3 TERHADAP KOMBINASI IMPLANT CONTROLLED INTERNAL DRUG RELEASE JANGKA PENDEK DENGAN INJEKSI PROSTAGLANDIN F2 ALPHA Dewi, Ratri Ratna; w, Wahyuningsih; Widayati, Diah Tri
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i1.418

Abstract

Penelitian ini bertujuan mengetahui respon estrus pada kambing Peranakan Ettawa (PE) dengan Body Condition Score (BCS) 2 dan 3 terhadap implant Controlled Internal Drug Release (CIDR) jangka pendek dikombinasikan dengan injeksi prostaglandin F alpha (PGF a). Sebanyak 10 ekor kambing PE betina dibagi 2 2dalam 2 kelompok perlakuan berdasarkan BCS, yaitu kelompok I dengan BCS 2 (kondisi kurus), berat badan antara 25-30 kg (n=5) dan kelompok II dengan BCS 3 (kondisi ideal) berat badan antara 35-40 kg (n=5). Semua kelompok perlakuan disinkronisasi dengan implant CIDR (berisi 1,3 g progesteron) secara intravaginal jangka pendek selama 10 hari dan 48 jam sebelum pencabutan CIDR, kambing diijeksi dengan PGF a. Deteksi estrus dilakukan setiap hari dengan interval 6 jam dimulai dari pencabutan CIDR sampai 60 2jam setelah pencabutan CIDR. Data onset dan durasi estrus dianalisis menggunakan independent sample T-test sedangkan tanda-tanda visual estrus dianalisis secara deskriptif. Respon estrus semua kelompokperlakuan adalah 100%. Onset dan durasi estrus antara BCS 2 dan 3 masing-masing adalah 21,601,47 vs 13,201,20 jam dan 27,603,06 vs 32,401,47 jam. Dari hasil penelitian dapat disimpulkan bahwa kombinasi implant CIDR jangka pendek dan injeksi PGF a efektif untuk sinkronisasi estrus pada kambing PE dengan 2BCS 2 dan 3.
CELLULAR MECHANISM UNDERLYING EMBRYO-MATERNAL RELATIONSHIP IN INTRASPECIFIC AND INTERSPECIFIC PREGNANCY widayati, Diah tri; Fukuta, Katsuhiro
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.273

Abstract

Mouse and vole embryos were transferred into pseudopregnant CD-1 and scid female mice. Cellular changes involved in the formation of decidua in the pregnant mouse uterus up to day 8 of pregnancy were examined by histological, electron microscopic, and histochemical techniques. On day 6 of pregnancy, the vole embryos were laid in interstitium of antimesometrial side of the uterus, as well as in intraspecific pregnancy. Compared with intraspesific pregnant mouse, blood vessels were numerous in the decidua around the vole embryos in interspecific pregnancy. Both distribution and dilation of the blood vessels were increased on day 8. A part of cells in the inner cell mass had not nuclei, suggesting damaged vole embryos on day 8. At the implantation site, the uterine decidua was invaded by extravillous trophoblast (EVT) cells whose function is to destroy the walls of the uterine spiral. Moreover, this decidua was infiltrated by a population of natural killer (NK) cells and macrophage. These cells were particularly numerous in the decidua basalis at the implantation site where they come into close contact with invading EVT cells. These results suggest that interaction between NK, macrophage, and EVT provides the controlling relationship of embryo-maternal in intraspecific and interspecific pregnancy.
The Influence of Parity on Blood Metabolite Profiles and Reproductive Performance in Saanen-Etawah Crossbred Does Diatmono, Dio Fico Felsidan; Paramita, Stefani Winda; Padmawati, Fransisca Gani; Sitaresmi, Pradita Iustitia; Kumala, Seraphina; Andi, Megawati; Widyobroto, Budi Prasetyo; Suranindyah, Yustina Yuni; Freitas, Joana da Costa; Widayati, Diah Tri
Buletin Peternakan Vol 49, No 4 (2025): BULETIN PETERNAKAN VOL. 49 (4) November 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i4.110480

Abstract

This study aimed to observe the effect of parity on the blood metabolite profiles and reproductive performance of Saanen-Etawah Crossbred does, maintained in smallholder farm. A total of 40 non-pregnant lactating does were divided into two groups: primiparous (n=16) and multiparous (n=24). The blood metabolite profiles, including total protein, glucose, cholesterol, and blood urea nitrogen (BUN), were assessed. Blood samples were collected from the jugular vein during the follicular phase, two hours prior morning feeding. Analysis of blood metabolites was carried out using photometric atomic absorption spectroscopy (AAS)-flame procedure. Furthermore, reproductive performance was observed through observing the characteristics of estrus and the length of the estrus cycle. The results indicated total protein in blood were significantly higher (p<0.05) in multiparous does (7.04±0.87 g/dL) compared to primiparous does (5.47±1.40 g/dL). In contrast, primiparous does exhibited significantly higher (p<0.05) blood cholesterol levels (122.33±30.55 mg/dL) than multiparous does (107.14±13.45 mg/dL). Multiparous does displayed typical estrus characteristics, including vulvar reddening, swelling, and vaginal mucus production, alongside a significantly shorter estrus cycle length. Parity was positively correlated with total protein levels (r=0.577), vulvar reddening (r=0.355), vulvar swelling (r=0.343), and vaginal mucus production (r=0.450), while negatively correlated with cholesterol levels (r=-0.330) and estrus cycle length (r=-0.532). These findings indicate that parity influences total protein and cholesterol levels, as well as several key estrus characteristics. Further investigation into optimized feed regimens and reproductive hormone fluctuations is warranted to enhance the reproductive efficiency of Saanen-Etawah Crossbred does.
Regulation of Reactive Oxygen Species by Sodium Selenite in AndroMed® Extender Enhances Post-Thaw Saanen Buck Semen Quality Erlindra, Caterina Palma; Diatmono, Dio Fico Felsidan; Padmawati, Fransisca Gani; Sastranegara, Arina Dinar Nurfadilla; Sitaresmi, Pradita Iustitia; Bintara, Sigit; Widayati, Diah Tri
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108422

Abstract

Semen cryopreservation is known to cause considerable damage to spermatozoa through the generation of free radicals. This damage can potentially be mitigated by the use of potent antioxidants, such as sodium selenite, which does not impair semen quality when administered in small doses. This study aimed to assess the impact of low-dose sodium selenite supplementation on post-thaw semen quality in Saanen bucks. Semen was collected from 2 superior Saanen bucks and evaluated according to standard criteria before dilution and freezing. Cryopreserved semen was stored in liquid nitrogen (LN2) at a temperature of -196 °C for 90 days. The experimental treatments involved the addition of sodium selenite to the AndroMed® diluent medium at 3 different concentrations: without supplementation/control (C), 5 ppm (T1), and 10 ppm (T2). Frozen semen was thawed at 37 °C in a water bath for 30 seconds before post-thaw quality assessment. The parameters assessed included motility, viability, abnormalities, plasma membrane integrity (PMI), acrosome membrane integrity (AMI), and reactive oxygen species (ROS). The data were statistically analyzed using One-Way analysis of variance (ANOVA). The results showed that the addition of sodium selenite had a significant effect (p < 0.05) on motility, viability, PMI, AMI, and ROS production, but did not affect the abnormality (p > 0.05). Interestingly, T1 resulted in the most substantial improvements, yielding the highest percentages of post-thaw motility (71.81±1.05%), viability (73.36±1.08%), PMI (77.49±1.68%), and AMI (79.29±0.63%), as well as the lowest ROS production (13.67±0.50%). In conclusion, the addition of 5 ppm sodium selenite enhances post-thaw semen quality in Saanen bucks.
Co-Authors Ahmad Pramono Aji, Riyan Nugroho Akhmad Fathoni Andi, Megawati Andriyani Astuti Arif Setyobudi Aris Junaidi Asmarani Kusumawati Atmoko, Bayu Andri Bambang Sugito Bambang Sugito Budi Prasetyo Widyobroto Cuk Tri Noviandi Devita Hery Fatmawati Devita Hery Fatmawati Dewi Pranatasari Diatmono, Dio Fico Felsidan Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwiviyanti, Elisa Elisa Dwiviyanti Endang Baliarti Erlindra, Caterina Palma Freitas, Joana da Costa Gustan Pari Gustan Pari Hari Hartadi I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Jaswadi J Jaswadi J Johan F. Koibur Joko Riyanto Katsuhiro Fukuta Katsuhiro Fukuta Kresno Suharto Kumala, Seraphina Kustono (Kustono) Kustono (Kustono) Kustono K Kustono K Kustono Kustono Kustono Kustono Laelatul Rahmah Lies Mira Yusiati Marcella Arka Paramita Masato Yoshida Masato Yoshida, Masato Maulana, Hamdani Mujtahidah Anggriani Ummul Muzayyanah Mukhamad Khusnudin Nono Ngadiyono Nono Ngadiyono Nony Ariesta Nony Ariesta Nurliyani (Nurliyani) Nurvina Septi Adifa P P Putro, P P Padmawati, Fransisca Gani Paramita, Marcella Arka Paramita, Stefani Winda PUDJI ASTUTI Rahmah, Laelatul Ratri Ratna Dewi Ratri Ratna Dewi Riyan Nugroho Aji Sastranegara, Arina Dinar Nurfadilla Seraphina Kumala Sigit Bintara Sitaresmi, Pradita Iustitia Sumadi Sumadi Sunendar (Sunendar) Sunendar - Tety Hartatik Tri Wahyu Pangestiningsih Trisakti Haryadi Wahyuningsih w Wahyuningsih w Widya Asmarawati Yanuar Achadri Yustina Yuni Surandiyah