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Diah Tri Widayati
Department Of Animal Breeding And Reproduction, Faculty Of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Energy Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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EVALUATION OF FRIESIAN HOLSTEIN GRADE COWS FERTILITY BASED THE LEVEL OF MILK UREA Widayati, Diah Tri; Suranindyah, Yustina Yuni; Rahmah, Laelatul; Widyobroto, Budi Prasetyo
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.4929

Abstract

This study was conducted to evaluate fertility grade of Friesian Holstein cows based on milk urea levels. A number of eight cows which fulfill the criteria of not pregnant and in second to sixth lactation, produced milk of 2.5 liters/day, and body weight ranging from 400-450 kg were used in this research. The cows were given fodder that consisted of 30 kg of forage and 10 kg of a mixture of concentrate (pollard, pulp out, and molasses), in the morning and afternoon. Milk and blood samples were taken once a week for five weeks in the morning before fed. Milk urea levels were measured using the urea FS (DiaSys) and blood samples were tested using enzyme linked immuno-sorbent assay (ELISA) with the progesterone hormone kit (DRG, Germany). The variables measured were pregnancy rate, service per conception (S/C), days open, and progesterone levels. Reproduction and milk urea levels data were analyzed by linear regression to determine the relationship between two variables. Progesterone data were analyzed descriptively. The results showed that the average calving interval, days open, and S/C were 607.5170.27 days; 341.25187.65 days; and 2.412.32 days, respectively. The average of milk urea levels was 14.162.55 mg/dL. Regression analysis showed a significant effect (P0.05) and negative correlation between milk urea level and S/C. The average of non-pregnant progesterone hormone level was 12.047.13 ng/mL. In conclusion, the fertility grade of Friesian Holstein cows decreased when the milk urea level at 14.16 mg/dL and non-pregnant progesterone at 12.047.13 ng/mL.
PENGGUNAAN CAIRAN FOLIKEL DALAM MEDIA MATURASI IN VITRO OOSIT KAMBING BLIGON Widayati, Diah Tri; Fatmawati, Devita Hery; Ariesta, Nony; K, Kustono
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1263

Abstract

Penelitian ini bertujuan mengetahui pengaruh medium maturasi terhadap kompetensi perkembangan in vitro oosit kambing bligon. Ovarium kambing diperoleh dari Rumah Potong Hewan, dibawa ke laboratorium dalam 0,9% NaCl pada suhu 31-34 C. Oosit diaspirasi dari folikel berdiameter 2-6 mm dengan menggunakan syringe 3 ml dan jarum 23 G. Oosit dengan minimal 2 lapisan sel-sel kumulus diproses untuk maturasi in vitro. Oosit dicuci 3 kali dengan dulbeccos phosphate buffered saline (DPBS) dan tissue culture medium 199 (TCM) 199, kemudian didistribusikan ke dalam 50 l medium maturasi yang berbeda, yaitu TCM 199 (tanpa suplementasi),
INJEKSI MEDIA KULTUR EMBRIO SUPERNATAN DALAM UTERUS UNTUK MENINGKATKAN ANGKA IMPLANTASI EMBRIO PADA MENCIT Widayati, Diah Tri; Sugito, Bambang; Pangestiningsih, Tri Wahyu; Kusindarta, Dwi Liliek; J, Jaswadi
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.931

Abstract

Penelitian ini bertujuan mengetahui pengaruh injeksi media kultur embrio supernatan ke dalam uterus mencit 2 hari sebelum transfer embrio (TE) terhadap angka implantasi, yang diindikasikan oleh adanya embrio dan leukemia inhibitory factor (LIF) pada uterus. Mencit jenis Swiss Albino dibagi menjadi 2 kelompok yakni kelompok perlakuan dan kelompok kontrol, masing-masing terdiri atas 30 mencit. Kelompok perlakuan mendapat injeksi media kultur embrio supernatan (MKES) dalam uterus 2 hari sebelum TE sedangkan kelompok kontrol mendapat injeksi media kultur embrio (MKE) dalam uterus 2 hari sebelum TE. Dua hari setelah TE (h6 kebuntingan), mencit diperfusi dengan menggunakan larutan para formaldehid 4% dan diambil uterusnya. Uterus diproses untuk blok parafin dan dipotong dengan mikrotom setebal 5 m. Preparat dideparafinisasi dan diproses imunohistokimia dengan kit ABC dan antibodi LIF. Preparat diperiksa di bawah mikroskop untuk melihat implantasi embrio, distribusi LIF, dan jumlah LIF di desidua dan non-desidua. Angka implantasi antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 52,77 dan 40,88% (P0,05). Jumlah LIF desidua pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 12,83 dan 8,83 (P0,05) sedangkan jumlah LIF di non-desidua antara kelompok perlakuan dan kelompok kontrol masing-masing adalah 8,00 dan 4,50. Dapat disimpulkan bahwa injeksi media kultur embrio supernatan 2 hari sebelum TE dapat meningkatkan angka implantasi pada mencit.
RESPON ESTRUS PADA KAMBING PERANAKAN ETTAWA DENGAN BODY CONDITION SCORE 2 DAN 3 TERHADAP KOMBINASI IMPLANT CONTROLLED INTERNAL DRUG RELEASE JANGKA PENDEK DENGAN INJEKSI PROSTAGLANDIN F2 ALPHA Dewi, Ratri Ratna; w, Wahyuningsih; Widayati, Diah Tri
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i1.418

Abstract

Penelitian ini bertujuan mengetahui respon estrus pada kambing Peranakan Ettawa (PE) dengan Body Condition Score (BCS) 2 dan 3 terhadap implant Controlled Internal Drug Release (CIDR) jangka pendek dikombinasikan dengan injeksi prostaglandin F alpha (PGF a). Sebanyak 10 ekor kambing PE betina dibagi 2 2dalam 2 kelompok perlakuan berdasarkan BCS, yaitu kelompok I dengan BCS 2 (kondisi kurus), berat badan antara 25-30 kg (n=5) dan kelompok II dengan BCS 3 (kondisi ideal) berat badan antara 35-40 kg (n=5). Semua kelompok perlakuan disinkronisasi dengan implant CIDR (berisi 1,3 g progesteron) secara intravaginal jangka pendek selama 10 hari dan 48 jam sebelum pencabutan CIDR, kambing diijeksi dengan PGF a. Deteksi estrus dilakukan setiap hari dengan interval 6 jam dimulai dari pencabutan CIDR sampai 60 2jam setelah pencabutan CIDR. Data onset dan durasi estrus dianalisis menggunakan independent sample T-test sedangkan tanda-tanda visual estrus dianalisis secara deskriptif. Respon estrus semua kelompokperlakuan adalah 100%. Onset dan durasi estrus antara BCS 2 dan 3 masing-masing adalah 21,601,47 vs 13,201,20 jam dan 27,603,06 vs 32,401,47 jam. Dari hasil penelitian dapat disimpulkan bahwa kombinasi implant CIDR jangka pendek dan injeksi PGF a efektif untuk sinkronisasi estrus pada kambing PE dengan 2BCS 2 dan 3.
CELLULAR MECHANISM UNDERLYING EMBRYO-MATERNAL RELATIONSHIP IN INTRASPECIFIC AND INTERSPECIFIC PREGNANCY widayati, Diah tri; Fukuta, Katsuhiro
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.273

Abstract

Mouse and vole embryos were transferred into pseudopregnant CD-1 and scid female mice. Cellular changes involved in the formation of decidua in the pregnant mouse uterus up to day 8 of pregnancy were examined by histological, electron microscopic, and histochemical techniques. On day 6 of pregnancy, the vole embryos were laid in interstitium of antimesometrial side of the uterus, as well as in intraspecific pregnancy. Compared with intraspesific pregnant mouse, blood vessels were numerous in the decidua around the vole embryos in interspecific pregnancy. Both distribution and dilation of the blood vessels were increased on day 8. A part of cells in the inner cell mass had not nuclei, suggesting damaged vole embryos on day 8. At the implantation site, the uterine decidua was invaded by extravillous trophoblast (EVT) cells whose function is to destroy the walls of the uterine spiral. Moreover, this decidua was infiltrated by a population of natural killer (NK) cells and macrophage. These cells were particularly numerous in the decidua basalis at the implantation site where they come into close contact with invading EVT cells. These results suggest that interaction between NK, macrophage, and EVT provides the controlling relationship of embryo-maternal in intraspecific and interspecific pregnancy.
The Influence of Parity on Blood Metabolite Profiles and Reproductive Performance in Saanen-Etawah Crossbred Does Diatmono, Dio Fico Felsidan; Paramita, Stefani Winda; Padmawati, Fransisca Gani; Sitaresmi, Pradita Iustitia; Kumala, Seraphina; Andi, Megawati; Widyobroto, Budi Prasetyo; Suranindyah, Yustina Yuni; Freitas, Joana da Costa; Widayati, Diah Tri
Buletin Peternakan Vol 49, No 4 (2025): BULETIN PETERNAKAN VOL. 49 (4) November 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i4.110480

Abstract

This study aimed to observe the effect of parity on the blood metabolite profiles and reproductive performance of Saanen-Etawah Crossbred does, maintained in smallholder farm. A total of 40 non-pregnant lactating does were divided into two groups: primiparous (n=16) and multiparous (n=24). The blood metabolite profiles, including total protein, glucose, cholesterol, and blood urea nitrogen (BUN), were assessed. Blood samples were collected from the jugular vein during the follicular phase, two hours prior morning feeding. Analysis of blood metabolites was carried out using photometric atomic absorption spectroscopy (AAS)-flame procedure. Furthermore, reproductive performance was observed through observing the characteristics of estrus and the length of the estrus cycle. The results indicated total protein in blood were significantly higher (p<0.05) in multiparous does (7.04±0.87 g/dL) compared to primiparous does (5.47±1.40 g/dL). In contrast, primiparous does exhibited significantly higher (p<0.05) blood cholesterol levels (122.33±30.55 mg/dL) than multiparous does (107.14±13.45 mg/dL). Multiparous does displayed typical estrus characteristics, including vulvar reddening, swelling, and vaginal mucus production, alongside a significantly shorter estrus cycle length. Parity was positively correlated with total protein levels (r=0.577), vulvar reddening (r=0.355), vulvar swelling (r=0.343), and vaginal mucus production (r=0.450), while negatively correlated with cholesterol levels (r=-0.330) and estrus cycle length (r=-0.532). These findings indicate that parity influences total protein and cholesterol levels, as well as several key estrus characteristics. Further investigation into optimized feed regimens and reproductive hormone fluctuations is warranted to enhance the reproductive efficiency of Saanen-Etawah Crossbred does.
Comparative Effects of Selenium and Gonadotropin Supplementation on In Vitro Maturation of Bligon Goat Oocytes Sastranegara, Arina Dinar Nurfadilla; Agustina, Rona; Diatmono, Dio Fico Felsidan; Padmawati, Fransisca Gani; Sitaresmi, Pradita Iustitia; Bintara, Sigit; Widayati, Diah Tri
Buletin Peternakan Vol 50, No 2 (2026): BULETIN PETERNAKAN VOL. 50 (2) May 2026 : In Progress
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v50i2.115278

Abstract

Selenium (Se) is a novel addition to in vitro maturation (IVM) media, unlike the traditional gonadotropin hormone. This highlights the advancements in reproductive biology and potential strategies to enhance oocyte maturation. This research was conducted to determine the effect of Se and gonadotropin hormone supplementation into maturation medium on the in vitro oocyte maturation of Bligon goat. Ovaries from Bligon goats were obtained from a slaughterhouse in Special Region of Yogyakarta. High-quality oocytes (grades A and B) were retrieved through aspiration, washed, and cultured in medium beneath mineral oil. Three experimental conditions were established: 50 μL of unsupplemented medium (T0), 48 μL of medium + 2 μL of gonadotropin (T1), and 48 μL of medium + 2 μL of Se (T2). The culture process was conducted for 24 h at 39°C, 95% humidity, and 5% CO2. Oocyte maturation was evaluated based on cumulus cell expansion, and the data obtained were analyzed using One-Way ANOVA, followed by Tukey’s Post Hoc test. The results indicated that the inclusion of either Se (86.26±7.20%) or gonadotropin (88.13±7.85%) in the IVM medium significantly enhanced (p<0.05) the maturation rate compared with that of the unsupplemented group (50.09±11.90%). However, there was no significant difference (p>0.05) between the two supplements. In conclusion, both Se and gonadotropin hormones proved to be effective supplements for improving the maturation rate of Bligon goat oocytes.
Co-Authors Agustina, Rona Ahmad Pramono Aji, Riyan Nugroho Akhmad Fathoni Andi, Megawati Andriyani Astuti Arif Setyobudi Aris Junaidi Asmarani Kusumawati Atmoko, Bayu Andri Bambang Sugito Bambang Sugito Budi Prasetyo Widyobroto Cuk Tri Noviandi Devita Hery Fatmawati Devita Hery Fatmawati Dewi Pranatasari Diatmono, Dio Fico Felsidan Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwiviyanti, Elisa Elisa Dwiviyanti Endang Baliarti Freitas, Joana da Costa Gustan Pari Gustan Pari Hari Hartadi I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Jaswadi J Jaswadi J Johan F. Koibur Joko Riyanto Katsuhiro Fukuta Katsuhiro Fukuta Kresno Suharto Kumala, Seraphina Kustono (Kustono) Kustono (Kustono) Kustono K Kustono K Kustono Kustono Kustono Kustono Laelatul Rahmah Lies Mira Yusiati Marcella Arka Paramita Masato Yoshida Masato Yoshida, Masato Maulana, Hamdani Mujtahidah Anggriani Ummul Muzayyanah Mukhamad Khusnudin Nono Ngadiyono Nono Ngadiyono Nony Ariesta Nony Ariesta Nurliyani (Nurliyani) Nurvina Septi Adifa P P Putro, P P Padmawati, Fransisca Gani Paramita, Marcella Arka Paramita, Stefani Winda PUDJI ASTUTI Rahmah, Laelatul Ratri Ratna Dewi Ratri Ratna Dewi Riyan Nugroho Aji Sastranegara, Arina Dinar Nurfadilla Seraphina Kumala Sigit Bintara Sitaresmi, Pradita Iustitia Sumadi Sumadi Sunendar (Sunendar) Sunendar - Tety Hartatik Tri Wahyu Pangestiningsih Trisakti Haryadi Wahyuningsih w Wahyuningsih w Widya Asmarawati Yanuar Achadri Yustina Yuni Surandiyah