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All Journal JURNAL KESEHATAN LINGKUNGAN: Jurnal dan Aplikasi Teknik Kesehatan Lingkungan Narra J Archives of The Medicine and Case Reports Jurnal Penyakit Dalam Indonesia Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal) International Journal of Biomedical Science and Travel Medicine
Erni Juwita Nelwan, Erni Juwita
Departemen Ilmu Penyakit Dalam, FK Universitas Indonesia RSUPN Dr. Cipto Mangunkusumo
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Narra J

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Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Benediktus Yohan; Erni Juwita Nelwan; R. Tedjo Sasmono
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati, Aryati; Sofro, Muchlis AU; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Yohan, Benediktus; Nelwan, Erni Juwita; Sasmono, R. Tedjo
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
Co-Authors Aditya Wardhana Adityo Susilo, Adityo Agus Santosa Agus Santosa Alvina Widhani, Alvina Antono, Akbarbudhi Aryati Aryati Aryati Aryati Asep Purnama Asep Purnama, Asep Aulia Rizka Aulia Rizka, Aulia Baso, Gery Dala Prima Benediktus Yohan Birry Karim Bramantya Wicaksana Ceva W. Pitoyo Cleopas Martin Rumende Cleopas Martin Rumende Cynthia Olivia Maurine Jasirwan David Hutajulu Dimas Priantono Dimas Priantono, Dimas Dono Antono Duindrahajeng, Bernadine Gracia E. Mudjaddid A. Siswanto Deddy N.W.Achadiono Hamzah Shatri Edwin Wijaya Eka Ginanjar Em Yunir, Em Evy Yunihastuti Fadhilah, Azzahra Firda Annisa Fransiscus Nikodemus Hosea Gery Dala Prima Baso Gondo Mastutik Gurmeet Singh Hakim, Manfaluthy Hamka Gunawan Marpaung Hasibuan, Anshari Saifuddin Herikurniawan Herikurniawan Hesty Utami Ramadaniaty Irianty Juana Anna Bertha Khie Chen Kumalawati, July Kuntaman Kuntaman Kuntjoro Harimurti Leonard Nainggolan Maradewi Maksum Marcellus Simadibrata Marpaung, Hamka Gunawan Mira Yulianti Muchlis AU Sofro Nabella Qisthina Laksita Dewi Olivia Jocelyn Pasaribu, Adeline Pringgodigdo Nugroho, Pringgodigdo Purnomo, Windu R. Tedjo Sasmono Retno S Wardani Retno S Wardani Retno Wahyuningsih Retno Wahyuningsih Riswan Riswan Robert Sinto Robert Sinto Rustam, Musfardi Sasmono, R. Tedjo Shakinah, Sharifah Siti Farida Sofro, Muchlis AU Sri Masyeni, Dewa Ayu Putri Suhendro Suwarto, Suhendro Sukamto Koesnoe Sulistianingsih, Dyah Purnamasari Suzy Maria Syamsudin Abdillah Usman Hadi Veronica, Raja Merlinda Wicaksana, Bramantya Widayat Djoko Santoso Windu Purnomo Yohan, Benediktus Yudianto, Dian