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All Journal AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX KESMAS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Reiny A. Tumbol
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Public Health

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Seleksi probiotik Lactobacillus sp. dari usus ikan mas (Cyprinus carpio) potensial untuk akuakultur Bella, Sintia S.; Manoppo, Henky; Undap, Suzanne L.; Tumbol, Reiny A.; Ngangi, Edwin L. A.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29902

Abstract

The aimed of the study was to isolate and select the probiotic Lactobacillus from carp intestine potential for use in aquaculture. Carp as a source of probiotics with a size of about 200 g was  taken from the Center for Fish Hatchery and Disease Control (BP3I), Tateli. After aseptic surgery, the intestine was separated and cleaned, weighed as much as 1 g, put in a mortar and crushed until smooth. The scoured results were put into a tube and added 9 ml NaCl to get a ratio of 1:10. The tube that already contains intestinal scouring was then centrifuged at a speed of 1000 RPM for 10 minutes. The centrifugation process was carried out 2 times to get the supernatant that has been cleared of debris. The supernatant was removed and diluted to obtain a concentration of 10-2  to 10-3. The solution was then spread on MRS (De Man Rogosa and Sharpe Agar) media and incubated at 28oC in the Incubator for 24-48 hours. The bacteria that grew in culture fmedia was reisolated several times to get a single pure colony.. The pure colonies were propagated in the same media to be further identified through gram staining, biochemical, proteolytic, amylolytic, cellulolytic, antibiotic resistance, antagonistic, pathogenicity tests. The results of the isolation obtained the characteristics of a colony of milky white color, round and convex with long cells, gram-positive and confirmed based on biochemical tests as Lactobaccilus sp. The bacterium also had proteolytic, amylolytic, cellulolytic, antagonistic, antibiotic-sensitive activity that were characterized by the formation of clear zones in the area around probiotic discs, and did not cause death either as a disease or as a result of poisoning. Thus it can be concluded that Lactobaccilus sp. isolated from carp intestines can be used as probiotics for cultivation.
Penggunaan probiotik untuk meningkatkan respon imun nonspesifik ikan mas (Cyprinus carpio) Undi, Chintia S.; Manoppo, Henky; Kreckhoff, Reni L.; Tumbol, Reiny A.; Pangkey, Henneke
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29908

Abstract

The aim of this research was to count the total leukocytes and phagocytosis activity of goldfish seeds treated with probiotics. The test fish used were goldfish seeds measuring 5-8 cm. 500 fish will be taken from the Tateli Fish Cultivation and Development Center (BP3I). This study used a completely randomized design (CRD) with 5 treatments, where each treatment had 3 replications. The treatments used were probiotic bacteria from the intestines of goldfish with different concentrations. The treatments tested consisted of: A: without the addition of probiotics, B: addition of probiotics 1x106 cfu / mL, C: addition of probiotics 1x107 cfu / mL, D: addition of probiotics 1x108 cfu / mL, E: addition of probiotics 1x109 cfu / mL. Each concentration of bacteria that has been prepared is then sprayed on the commercial feed evenly using a sprayer and then dried to room temperature. After drying the feed was coated with 2% egg yolk and dried again at room temperature. Fish are stocked in an aquarium measuring 60x40x40cm3 with a density of 20 fish / aquarium. Each aquarium is equipped with an aerator and a water pump for recirculation. The fish were then given feed that had been added with probiotics at the same dose and frequency of administration as in the acclimatization process. The treatment is given for 6 weeks. At the end of the treatment feeding, fish samples were taken from each experimental unit to measure immune parameters, namely total leukocytes and phagocytic activity. Based on the results of research using the probiotic Lactobacillus sp. which originated from the intestines of goldfish and added to the feed had a significant effect on increasing total leukocytes and phagocytosis activity and the optimal concenration was  1x107 - 1x108 cfu / mL with a duration of 6 weeks of administration
Efektivitas ekstrak daun akar kucing (Acalypha indica) dalam meningkatkan pertumbuhan ikan Nila, Oreochromis niloticus Ratusmanga, Jihan; Hutagaol, Anggun F.; Manoppo, Henky; Lantu, Sartje; Tumbol, Reiny A.; Mokolensang, Jeffrie F.; Kaligis, Erly Y.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35531

Abstract

The purposes of research were to examine the effect of extract of Acalypha indica leaf on the growth of Nile tilapia and determine the dose of leaf extract that could enhance the growth of tilapia optimally. The fish used were Nile tilapia taken from the Tatelu Center for Freshwater Aquaculture with an average weight of 3.79±0.50 g. The medicinal plant A. indica was collected from around the Faculty of Fisheries and Marine Science complex and from the North Bolaang Mongondow area. After being washed, the leaves were dried and then ground to obtain leaf powder. Leaf flour was then extracted by maceration method with 95% alcohol solvent. After being concentrated, the extract was then used as a treatment with different concentrations, including A: 0 g extract/kg feed, B:10 g extract/kg feed, C:20 g extract/kg feed, D:30 g extract/kg feed and E: 40g extract/kg feed. The data collected consisted of absolute growth, daily growth and relative growth. The results showed that the extract of A. indica leaf added to the feed had a significantly effect on the growth of tilapia indicated by an increase in absolute growth, daily growth, and relative growth (p<0.05). The best fish growth was achieved in fish fed with feed with the addition of 20 g extract/kg feed. In conclusion, leaf extract of A. indica can increase the growth of Nile tilapia
Identifikasi bakteri patogen Aeromonas sp. pada ikan Nila (Oreochromis niloticus) di Desa Matungkas, Kecamatan Dimembe, Kabupaten Minahasa Utara Sinubu, Wulan V.; Tumbol, Reiny A.; Undap, Suzanne L.; Manoppo, Henky; Kreckhoff, Reni L.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36633

Abstract

The purpose of this study was to identify the bacteria Aeromonas sp. in a tilapia (Oreochromis niloticus) culture pond in Matungkas Village, Dimembe District, North Minahasa Regency. Bacterial identification was carried out at the Laboratory of the Fish Quarantine Center for Quality and Safety Control of Fishery Products, Manado. The time of the study was carried out from February 2021 - June 2021. Ten samples of fish were selected from 3 existing ponds for this study with the criteria of fish that have clinical signs of disease infection such as pale body surface, red spots and ulcers. From 10 samples, 19 isolates were obtained to be identified. The bacterial method was carried out based on the results of gram tests and biochemical tests which included: Gram Tests, Motility Test, Oxidase Test, Oxidative Fermentative Test, Tolerance Test to incubation temperature, Tolerance Test to pH of the media, Tolerance Test to the concentration of NaCl in the media and rimler – shotts selective media test. Of the 19 isolates from 10 samples identified, there were 7 isolates that were positive for A. hydrophila, namely: N1.4.1, N2.1.1, N2.1.2, N2.2.1, N2.3.2, N3.3.1, and N3.3.2. Twelve other isolates were negative for A. hydrophila. The characters of A. hydrophila identified are creamy in colour, gram negative, oxidase positive, bacteria tolerance to temperature 4 °C positive, 37 °C positive, 50 °C negative, can move (motile), fermentative, pH tolerance test media pH 3 negative, pH 5 positive, pH 9 positive, pH 11 positive, tolerance test for NaCl concentration 0.5% positive, 3% positive, 5% negative, rimler – shotts positive. All sampling sites (ponds) in Matungkas Village were infected with bacteria.
Penggunaan ekstrak Ciplukan (Physalis angulata) untuk memacu pertumbuhan ikan Nila (Oreochromis niloticus) Maweikere, Fabiola C.; Tumbol, Reiny A.; Monijung, Revol D.; Manoppo, Henky; Kreckhoff, Reni L.; Darwisito, Suria
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36635

Abstract

This study aimed to examine the effect of adding ciplukan (Physalis angulata) to feed on the growth of tilapia and to find a dose of ciplukan that was able to increase the growth of tilapia (Oreochromis niloticus). The test fish used were tilapia with an average size of 5-8 cm as many as 300 fish from the Center for Freshwater Aquaculture (BPBAT), Talelu. This research was conducted at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University. This study used a completely randomized design (CRD) with 4 treatments, where each treatment had 3 replications so there were 15 experimental units, the treatment used was ciplukan flour with different concentrations consisting of: A: 0 g flour/kg feed, B : 10 g flour/ kg feed, C : 15 g flour/ kg feed, D : 20 g flour/ kg feed. The data collected consisted of fish weight to measure the fish growth. The results showed that the addition of ciplukan leaf extract in feed with different concentrations gave significantly different effects on the growth of tilapia with treatment B (10 g) showing the highest fish growth reaching 5.24 g, followed by treatment C (15 g) with the highest growth. reached 4.35 g. While the fish treated with D (20 g) only reached 3.16 g in weight where this value was not much different from the growth of fish in control A. Duncan's further test results showed that treatments B, C, D were not significantly different from each other, but these three treatments were very significantly different compared to fish that were not given ciplukan leaf extract. The study showed that ciplukan leaf extract added to feed gave significantly different effects on fish growth, with the best dose of 10 g extract/kg feed. Thus it can be concluded that ciplukan leaf extract can increase growth of Nile tilapia.
Kajian kualitas air untuk menunjang perikanan budidaya Danau Moaat, Provinsi Sulawesi Utara Paputungan, Firgiawan; Pangemanan, Novie P. L.; Tumbol, Reiny A.; Undap, Suzanne L.; Tumembouw, Sipriana S.; Rantung, Steelma V.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.37130

Abstract

The purpose of this study was to measure several physical and chemical parameters of Mooat Lake waters and to assess the feasibility of water quality in Mooat Lake waters to support aquaculture.Primary data were obtained from direct sample measurements in the field (in situ) and laboratory analysis. In situ sample measurement data consisted of temperature, degree of acidity (pH), dissolved oxygen (DO), and turbidity. Sampling of water at all research stations used a modified bottle that was labeled accordingly as a water sample holder. After the water sample is taken in the field, stored in a cooler, then taken to the Laboratory of Environmental Health Engineering and Disease Control (BTKLPP) class 1 Manado and the Manado BARISTAND Laboratory for examination of levels of Nitrite (NO2), Nitrate (NO3), Ammonia (NH3) , and Phosphate (PO4). Based on research data of several water quality parameters measurements and analysis show that temperature (26,17oC), Turbidity (11,20 NTU), pH (5,6), DO (8,31), nitrite (0,04005 mg/l), nitrate (0,705 mg/l), ammonia (0,145 mg/l), and phosphate (1,005 mg/l). These results show that the waters of Mooat Lake is still feasible and meets the water quality standard values to support aquaculture activities. Furthermore, it is strengthened by the analysis of the suitability of the location for the development of aquaculture which shows that the water quality conditions of Mooat Lake are classified as suitable.
Identifikasi, Tingkat Insidensi, Indeks Dominasi dan Tingkat Kesukaan Parasit pada Sidat (Anguilla marmorata) Reiny A. Tumbol; Sammy N. Longdong; Tauvan A. Kanoli
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.66

Abstract

Penelitian ini dilakukan dengan bertujuan mengetahui jenis-jenis parasit, tingkat insidensi, indeks dominasi dan tingkat kesukaan parasit pada sidat (Anguilla marmorata). Pemeriksaan sampel Sidat dimulai tanggal 25 Mei−16 Juni 2009, di Laboratorium Kesehatan Ikan Balai Budidaya Air Tawar Tatelu. Sampel diambil secara acak dari kolam-kolam pembesaran sebanyak 50 ekor, ukuran panjang 30–45 cm. Pemeriksaan parasit pada Sidat terbagi atas pemeriksaan ektoparasit yaitu mata, mulut, kulit/sisik, insang dan sirip. Pemeriksaan endoparasit meliputi pemeriksaan lambung, usus, hati dan daging. Hasil identifikasi parasit yang menginfeksi Sidat adalah Trichodina sp, Lerneae sp, Myxobolus sp, Gyrodactylus sp, Oxyurida sp, Capillaria, Acanthocephalus sp dan Vorticella sp. Tingkat insidensi dari tiap spesies parasit adalah sebagai berikut; Trichodina sp 92%, Gyrodactylus sp 74%, Lerneae sp 26%, Capillaria sp 24%, Myxobolus sp 22%, Oxyurida sp 6%, Acanthocephalus sp 2% dan Vorticella 2%. Analisis indeks dominasi menunjukan bahwa ada satu jenis parasit yang mendominasi infeksi parasit-parasit yang ada pada organ-organ sampel yang diperiksa dan parasit tersebut adalah Trichodina Sp. Setiap jenis parasit memiliki tingkat kesukaan yang berbeda dalam menempati organ-organ tubuh Sidat.
Uji fitokimia dan aktivitas antimikroba ekstrak kulit pisang kepok (Musa paradisiaca) terhadap bakteri Aeromonas hydrophila Aylifia S. Somba; Reni L. Kreckhoff; Diane J. Kusen; Henky Manoppo; Reiny A. Tumbol; Fitje Losung
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 1 (2023)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.11.1.2023.42192

Abstract

This research was conducted to qualitatively analyze the phytochemical content of kepok banana (Musa paradisiaca) peel extract and the effectivity of the extract as antibacterial against Aeromonas hydrophila. Fresh yellow color banana peels were washed, drained, then cut into pieces, crushed and dried at room temperature. Extraction was carried out by maceration method with 70% and 95% ethanol as solvent. A total of 90 g was put in an elemeyer glass then added with solvent until all the banana peels were submerged. The immersion time was 3x24 hours at room temperature while stirring occasionally so that the extraction occurs properly. Furthermore, the extract was filtered with Whatman paper no. 42 to separate the residue from the filtrate. The filtrate was then concentrated using a rotary evaporator. Phytochemical examined consisted of alkaloid, flavonoid, sternoid, triterpenoid, saponin, and tannin. Inhibition test against A. hydrophila was carried out by disc diffusion method using 5 different extract concentrations as treatments, namely A: 0%, B:10%, C:20%, D:30%, E:40%, F:50%. In the inhibition test, the paper disc was dipped in each treatment and then placed on agar media previously stocked with bacteria. The inhibitory zone was measured based on the clear area formed around the disc and observed at 24, 48, and 72 hours. Phytochemical test found that phenol and tannin were only detected in extracts with 95% ethanol while terpenoids and steroids were only found in  ethanol70%.  Flavonoid was detected in the extract using either ethanol70% and 95%. The results of the inhibitory test showed that the kepok banana peel extract could inhibit the growth of A. hydrophila bacteria after 48 hours of incubation. Inhibition zones formed at both 48 hours and 72 hours tended to increase in line with the increase in concentration even though the inhibitory power produced was categorized as "medium".
Evaluation of the effect of probiotic bacteria on growth performance and survival rate of carp, Cyprinus carpio jeane T Sumaraw; Henky Manoppo; Reiny A. Tumbol; I.F.M Rumengan; Henny A. Dien; Deiske A. Sumilat
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23216

Abstract

The objective of this research was to obtain probiotic bacteria isolated from the digestive tract of catfish Clarias batracus, to evaluate its effect on growth performances and survival rate of carp. After purified, the bacteria was identified through biochemical tests and selection was carried out through proteolytic, amylolytic, cellulolytic, tests as well as pathogenicity test. Probiotic candidate was administered orally to fish at different concentrations. The fish were fed for 30 days respectively as much as 3% of body weight per day with feeding frequency of two times a day. The results showed that addition of probiotics into feed significantly increased growth performance but not survival rate.Keywords: Probiotic, Clarias batracus,  growth performance, aquaculture. ABSTRAKTujuan dari penelitian ini adalah untuk mendapatkan bakteri probiotik yang diisolasi dari saluran pencernaan ikan lele Clarias batracus, dan mengevaluasi pengaruhnya terhadap kinerja pertumbuhan dan kelangsungan hidup benih ikan mas. Bakteri probiotik diidentifikasi melalui uji biokimia dan seleksi dilakukan melalui proteolitik, amilolitik, tes selulolitik dan patogenisitas. Kandidat probiotik diberikan pada ikan secara oral dengan konsentrasi berbeda Pemberian pakan berprobiotik berlangsung selama 30 hari sebanyak  3% dari berat badan per hari dengan frekuensi pemberian dua kali sehari. Hasil penelitian menunjukkan bahwa penambahan probiotik ke dalam pakan secara signifikan meningkatkan kinerja pertumbuhan namun tidak memengaruhi kelangsungan hidup benih ikan mas.Kata kunci : Probiotik, Clarias batracus, kinerja pertumbuhan, budidaya
The use of Sponge Crude Extract to Increase Growth and Immune Response of Nile Tilapia Oreochromis niloticus Merlyn D. Manurung; Reiny A. Tumbol; Henneke D. Pangkey; Deiske A. Sumilat; Remy E. P. Mangindaan
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.23228

Abstract

This study aims to examine the effect of sponge crude extract addition to the fish feed on growth and non-specific immune response of nile tilapia (O. niloticus) and determine the most effective dose of the extract in raising the growth and the non-specific immune response of nile tilapia (O. niloticus). The sponge sample was macerated and then evaporated using a rotary vacuum evaporator until the sample became a paste and mixed with feed. The fish were fed for 14 days respectively as much as 5% of body weight per day with feeding frequency of two times a day. The results showed that addition of sponge crude extract to the fish feed gave good effect on the growth and the non-specific immune response of nile tilapia (O. niloticus) with best dose of  40 g / kg feed.Keywords: Sponge extract, fish immune response, fish growth, immune response, immunostimulant. ABSTRAKPenelitian ini bertujuan untuk menguji pengaruh penambahan ekstrak kasar spons pada pakan ikan terhadap pertumbuhan dan respon imun  non spesifik ikan nila (Oreochromis niloticus) serta menentukan dosis ekstrak kasar spons yang paling efektif dalam meningkatkan pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus). Sampel spons dimaserasi kemudian dievaporasi menggunakan rotary vacuum evaporator sehingga diperoleh ekstrak kasar berbentuk pasta yang kemudian dicampurkan pada pakan. Ikan uji diberi pakan perlakuan 5% dari bobot biomassa selama 14 hari dengan frekuensi pemberian pakan dua kali sehari. Hasil penelitian menunjukan bahwa penambahan ekstrak kasar spons pada pakan ikan memberikan pengaruh yang baik terhadap pertumbuhan dan respon imun non spesifik ikan nila (Oreochromis niloticus), yang mana dosis yang terbaik adalah 40 gr / Kg pakan.Kata Kunci : Ekstrak spons, respon imun ikan, pertumbuhan ikan, respon imun, imunostimulan.
Co-Authors Aylifia S. Somba Bella, Sintia S. Cyska Lumenta Deiske A. Sumilat Diane J. Kusen Edwin D Ngangi Erly Y. Kaligis, Erly Y. Feny Mentang Fitje Losung Galih Arif Kusuma Ginting, Elvi L. Haryadi Wijaya Hengky J. Sinjal Henky Manoppo Henky Manoppo Henneke D. Pangkey Henneke Pangkey Henny A Dien Henny A. Dien Hutagaol, Anggun F. I.F.M Rumengan Indra R.N. Salindeho jeane T Sumaraw Jimmy Mamuaya John L. Tombokan Jootje M. L. Umboh Jr, Arfiandi Juliaan Ch. Watung Kanoli , Tauvan A. Kreckhoff, Reni L. Labudo, Lipna Lauluw, Dafid Longdong, Sammy N. Mantiri, Desy M. H Manumpil, Silvana Markus T. Lasut Massora Dudung Maweikere, Fabiola C. Merlyn D. Manurung Mokoginta, Leyda H. Mokolensang, Jeffrie F. Monijung, Revol D. Mudeng, Cindy C. Ockstan J. Kalesaran Olvi A.P. Janis, Olvi A.P. Pangalila, Novelia M.A. Pangemanan, Novie P. L. Pangemanan, Novie P.L. Pankie N.L Pangemanan Paputungan, Firgiawan Prislawati Paputungan Putra, Stevano F.P Ratusmanga, Jihan Remy E. P. Mangindaan Sambali, Hariyani Sammy Longdong, Sammy Sammy N. J. Longdong Sammy N. Longdong Sammy N.J. Longdong Sartje Lantu Shifa A. Schram Sinubu, Wulan V. Sipriana S. Tumembouw Steelma V. Rantung Suria Darwisito, Suria Suzanne L Undap Suzanne L. Undap Tauvan A. Kanoli Telaumbanua, Beatus H. Umasugi, Aisin Undi, Chintia S. Veibe Warouw Wantah, Monika M. Winda M. Mingkid Winda M. Mingkid, Winda M.