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The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth Galih Arif Kusuma; Sammy N. J. Longdong; Reiny A. Tumbol
Jurnal Ilmiah PLATAX Vol. 2 No. 2 (2014): EDISI MEI - AGUSTUS 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.2.2.2014.7147

Abstract

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.
Water quality management for aquaculture at Lake Tutud, North Sulawesi Suzanne L. Undap; Reiny A. Tumbol
Jurnal Ilmiah PLATAX Vol. 4 No. 2 (2016): EDISI JULI-DESEMBER 2016
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.4.2.2016.15225

Abstract

Southeast Minahasa Regency has potential natural resources, one of them related aquculture in Lake Tutud. Considering the conditions and opportunities for the development of aquaculture in Lake Tutud, improvement efforts and increase the potential for fish production needs to be done. Some of the factors that caused the current problems include declining water level of Lake Tutud drastically in recent years caused by the death of most of spring water coming into the lake causing sedimentation, high content of sulfur from the irrigation run off. All of these problems can be a barrier to tilapia fish culture, so it is necessary to periodically measure the water quality in Lake Tutud in order to improve and enhance the quality of the water before it is used for culture efforts. Referring to the current economic conditions of the community around Lake Tutud of Tombatu 3 Village include community leaders and the fish farmers are also public observers who are interested in this program, through the Program Implementation of science and technology for the Community (IbM) of PNBP UNSRAT given counseling, training and mentoring such as water quality management technology. The methods used in the implementation of science and technology program are education, training, discussion and mentoring. Materials used were proper method of producing fish seeds, the stages of the production process, the use immunostimulatory in fish feed for fish disease management, maintain and improve water quality. With the Community Service Program through the application of science and technology, it is expected that there will be a transferred in science and technology in water quality management of Tutud lake so hopefully their business development, creation of business opportunity, income generation and welfare of the people in this Tombatu 3 village. The results revealed that the implementation of this community service activity in the form of the application of science and technology are: lecture activities, training, discussion and question and answer, including the lake water quality measurements that have been going well with good response of the fish farmers indicated by the presence of participants as much as 80%, show that a). increased production of tilapia fish farmers through water quality management can be implemented, b). tilapia fish farming is feasible and can provide profit if done properly, so it can be an alternative in an effort to help the reduction of unemployment and poverty. Interaction with the community on this community service activity, it is found that there were many people who still do not understand much about the overall material included engineering, water quality management and proper fish feed and feeding method. One of the barriers was the language, so it is necessary for the lectures and trainers to use the local language. It can be recommended in the future to keep on doing this kind of community service considering the inherent of the knowledge and application of water quality management is still a new thing and has a business prospective opportunities in the Tombatu 3 village. Keywords: water quality, community, management. Abstrak Kabupaten Minahasa Tenggara mempunyai potensi sumber daya alam yang banyak salah satunya yang berkaitan dengan bidang Perikanan budi daya air tawar adalah Danau Tutud. Memperhatikan kondisi dan peluang pengembangan pembudidayaan ikan di Danau Tutud maka upaya perbaikan dan peningkatan potensi produksi ikan perlu dilakukan. Budi daya ikan khususnya di Danau Tutud adalah pembudidayaan pembesaran ikan nila di Keramba Jaring Tanjap (KJT). Dalam usaha pengembangan budi daya ikan air tawar harus diimbangi dengan pengelolaan kualitas air danau dan penanganan penyakit ikan yang baik. Oleh sebab itu aspek-aspek yang menjadi factor pembatas dalam hal peningkatan produksi perlu dicari jalan keluarnya yang baik. Beberapa faktor yang menjadi masalah yang dihadapi saat ini antara lain adalah menyusutnya secara drastis permukaan air Danau Tutud dalam beberapa tahun belakangan ini akibat dari matinya sebagian besar mata air yang masuk ke danau sehingga mengalami sedimentasi, selain itu kandungan sulfur atau belerang cukup tinggi dari air irigasi yang berasal dari danau yang mana akan dapat menjadi hambatan usaha pembenihan ikan nila, sehinggan diperlukan adanya pengukuran kualitas air Danau Tutud untuk dapat memperbaiki dan meningkatkan kualitas air sebelum dipergunakan untuk usaha budi daya. Mengacu pada kondisi tersebut maka untuk masyarakat sekitar Danau Tutud di Desa Tombatu 3 antara lain para tokoh masyarakat dan para pembudidaya ikan juga masyarakat pemerhati yang tertarik pada program ini, melalui Program Penerapan Ipteks bagi Masyarakat (IbM) dari PNBP UNSRAT diberikan penyuluhan, pelatihan dan pendampingan berupa teknologi pengelolaan kualitas air. Metode yang digunakan dalam Program Penerapan Ipteks ini adalah penyuluhan, pelatihan, diskusi dan pendampingan. Materi kegiatan pengabdian penerapan Ipteks ini meliputi cara memproduksi benih ikan yang benar dan baik, tahapan-tahapan proses produksi pakan ikan yang diberi imunostimulan untuk penanganan penyakit ikan, tahapan tahapan untuk memelihara dan meningkatkan kualitas air. Dengan Program Pengabdian Kepada Masyarakat melalui penerapan Ipteks ini diharapkan dapat ditransfer ilmu dan teknologi pengelolaan kualitas air Danau Tutud sehingga diharapkan adanya pengembangan usaha, penciptaan peluang berusaha, peningkatan pendapatan dan kesejahteraan masyarakat di Desa Tombatu 3 ini. Hasil pelaksanaan kegiatan pengabdian kepada masyarakat dalam bentuk penerapan ipteks ini adalah: kegiatan ceramah, pelatihan, diskusi dan tanya jawab, termasuk dengan pengukuran kualitas air Danau Tutud telah berjalan dengan baik respon kehadiran peserta sebanyak 80%. Dari hasil pelatihan, diskusi tanya jawab dan prakek dilapangan menunjukkan bahwa a). peningkatan produksi pembudidaya ikan nila melalui pengelolaan kualitas air ini dapat dilaksanakan, b). pembudidayaan ikan nila ini layak dan dapat memberikan profit bila dilaksanakan dengan baik dan benar, sehingga dapat menjadi alternatif lapangan usaha baru untuk membantu program pengentasan penggangguran dan penanggulangan kemiskinan. Dari hasil interaksi dengan masyarakat pada kegiatan pengabdian ini diketahui bahwa banyak masyarakat yang masih belum banyak mengerti tentang keseluruhan materi termasuk pada materi teknik dan proses pengelolaan kualitas air dan pakan ikan yang baik dan benar, salah satu kendala adalah bahasa, sehingga perlu ceramah dan pelatihan yang menggunakan bahasa setempat. Untuk selanjutnya dapat direkomendasikan agar terus digalakkan dan dilakukan pembinaan yang melekat mengingat pengetahuan dan penerapan pengelolaan kualitas air ini masih merupakan hal yang baru dan merupakan peluang usaha yang prospektif di Desa Tombatu 3. Keywords: Kualitas Perairan, Komunitas, Manajemen.
The Use Of Marine Sponge Crude Extract To Improve The Resistance Of Tilapia Fish (Oreochromis niloticus) To Streptococcus agalactiae Infections Shifa A. Schram; Reiny A. Tumbol; Reni L. Kreckhoff
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23723

Abstract

This study aims to examine the effect of the use of crude marine sponge extract on the resistance of streptococcus agalactiae infection in tilapia, Oreochromis niloticus, and to establish the effective dose of crude sponge extract in improving the immune system and the growth of the fish. The sponge used in the study was Cribrochalina sp. taken from Malalayang waters, Manado. The fish were taken from Freshwater Aquaculture Center, Tatelu. The fish were acclimatized for a week. After being acclimatized the fish were given feed added with sponge crude extract as a treatment with different concentrations of 20 g, 40 g and 60 g / Kg of feed for 14 days as much as 5% / body weight / day with the frequency of feeding twice a day at 10:00 am and at 5:00 p.m. After being treated, the fish was challenged with S. agalactiae. The data collected consisted of tilapia resistance, Total Leukocyte Count (TLC) as immune parameters and absolute growth. The results showed that the addition of crude extracts of Cribrochalina sp. into feed can increase TLC and growth of tilapia (p <0.05). The best results were achieved in fish fed with the addition of sponge crude extract of 40 g/kg feed. The survival rate of tilapia fed with treatment diet then challenged with pathogenic bacteria S. agalactiae showed the best results (100% survival rate) compared to controls (75%). In conclusion, feeding with a crude extract of Cribrochalina sp. has the potential to increase the immune system and growth of tilapia.Keywords: Crude Extract, Marine Sponges, Cribrochalina sp., Tilapia, Resistance, Streptococcus agalactiaeABSTRAKPenelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak kasar spons laut terhadap resistensi ikan nila dalam menghadapi serangan Streptococcus agalactiae, mengidentifikasi spons yang digunakan, serta mengukur pengaruh serta menetapkan dosis pemberian ekstrak kasar spons untuk meningkatkan sistem imun dan pengaruhnya terhadap pertumbuhan ikan nila. Spons yang digunakan dalam penelitian adalah spons Cribrochalina sp. yang diambil dari perairan Malalayang. Ikan uji diambil dari Balai Budidaya Air Tawar Tatelu, Provinsi Sulawesi Utara. Ikan diaklimatisasi selama seminggu. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan dengan ekstrak kasar spons sebagai perlakuan dengan konsentrasi berbeda yaitu 0 g, 20 g, 40 g dan 60 g/kg pakan selama 14 hari sebanyak 5%/berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 10.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan, ikan diuji tantang dengan bakteri S. agalactiae. Data yang dikumpulkan terdiri dari kelangsunganhidup ikan nila. Total leukosit sebagai parameter imun dan pertumbuhan mutlak. Hasil penelitian mendapatkan bahwa penambahan ekstrak kasar spons Cribrochalina sp. ke dalam pakan mampu meningkatkan total leukosit dan pertumbuhan ikan nila (p<0.05). Dimana hasil terbaik dicapai pada ikan yang diberi pakan dengan penambahan ekstrak kasar spons sebanyak 40 g/kg pakan. Kelangsungan hidup ikan nila yang diberi pakan perlakuan yang diuji tantang dengan bakteri patogen menunjukkan hasil yang paling baik (tingkat kelangsungan hidup 100%) dibandingkan dengan kontrol (75%). Sebagai kesimpulan bahwa pemberian pakan dengan ekstrak kasar spons Cribrochalina sp. berpotensi untuk meningkatkan sistem kekebalan tubuh dan pertumbuhan pada ikan nila.Kata Kunci: Ekstrak kasar, Spons laut, Cribrochalina sp., Tilapia, Resistensi, Streptococcus agalactiae
The Survival Rate and Growth of Juvenile Crayfish (Cherax quadricarinatus) With Different Types of Shelter Jimmy Mamuaya; Winda M. Mingkid; Ockstan J. Kalesaran; Hengky J. Sinjal; Reiny A. Tumbol; John L. Tombokan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24510

Abstract

An experiment was designed to assess the relative performance of three shelter types on the survival and growth of red-claw crayfish (Cherax quadricarinatus).  A 13 days old juvenile crayfish with an average length of 10 mm were cultured in 400 ml water of glass bowl and were provided with one of 3 types of shelter over 19 days.  The various shelter types assessed were dried coconut leaves, bamboo and Hydrilla.  Juveniles in each bowl were fed with Artemia twice a day morning and in the afternoon. There was no significant effect (P>0.05) of shelter type on the survival rate; however, there was a significant effect (P<0.05) on growth with dried coconut leaves (5.33%) performing significantly better than Hydrilla (2.33%) and bamboo (2.00%).  This experiment showed that the shelter types provided had a significant effect on the growth but not in their survival.Keywords: Survival rate, Growth, juvenile Cherax quadricarinatus, ShelterABSTRAKTujuan penelitian ini adalah untuk menganalisis sintasan hidup dan  pertumbuhan  juvenil lobster air tawar (C. quadricarinatus) dengan shelter yang berbeda. Juvenil lobster yang berumur 13 hari dengan panjang rata rata 10 mm dipelihara dalam wadah toples kaca dengan diberikan ketiga perlakuan selama 19 hari.  Ketiga jenis perlakuan adalah daun kelapa kering, bambu dan Hydrilla.  Juvenil diberi pakan Artemia 2 kali sehari yaitu pada pagi dan sore hari.  Hasil penelitian menunjukkan ketiga perlakuan tidak berbeda nyata terhadap sintasan hidup (P>0.05), namun berbeda nyata (P< 0.05 ) untuk pertumbuhan dengan daun kelapa kering (5.33%), Hydrilla (2.33%) dan bambu (2%).     Penelitian ini menunjukkan bahwa jenis shelter memberikan pengaruh nyata  terhadap pertumbuhan namun tidak berbeda nyata untuk sintasan hidup.Kata kunci: Sintasan hidup, Pertumbuhan, Juvenil Cherax quadricarinatus, Shelter
Inventarisasi dan identifikasi ektoparasit yang menginfeksi benih ikan Nila (Oreochromis niloticus) Massora Dudung; Reni L. Kreckhoff; Reiny A. Tumbol; Sammy N.J. Longdong; Winda M. Mingkid
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.49945

Abstract

This study aimed to identify the parasites in tilapia seeds cultivated by the Matelenteng Fish Cultivation Group in Tumaluntung Village, as well as to analyze the prevalence, incidence, dominance index and preference level of parasites in tilapia seeds. This research was conducted from May to July 2023 in Tumaluntung Village, Kauditan District, North Minahasa Regency, North Sulawesi Province. Parasite examination was carried out on 15 samples of tilapia seeds which included the skin, fins, and gills of the fish. The fish samples were taken from the Matelenteng fish cultivation location in Tumaluntung Village, Kauditan District, North Sulawesi Province. The fish were taken from 1 breeding pond and put into plastic bags containing water, then given oxygen, and then brought to the Aquaculture Technology Laboratory of FPIK Unsrat for examination. Before the examination was carried out, the test fish were put into a bucket and given aeration so that the tilapia seeds remained alive until they were examined. Next, the length of the tilapia seeds was measured using a ruler. Examination of the tilapia seed samples was carried out using the smear preparation method where the examined organ was scraped, and the results of the scraping were placed in a Petri dish before being smeared on a prepared glass slide. Parasite examination was conducted using a microscope with a magnification of 100x. The inventory and identification results found 2 types of parasites, namely Epistylis sp. with 13 individuals in 3 samples of tilapia seeds and Dactylogyrus sp. with 2 individuals in 2 samples of tilapia seeds infecting the tilapia seeds. The prevalence rate of Epistylis sp. parasites was obtained at 33.33% or generally, there is a mild infection. Dactylogyrus sp. with a prevalence rate of 13.33% is classified as often infected with an incidence rate at a very mild level. The preference level of parasites obtained a value of X2 hit 3.74 < X2 tab 9.210 indicating a difference in the preference level of parasites in the tilapia seed body organs. Keywords: Ectoparasites, prevalence, incidence, preference level
Tingkat prevalensi dan intensitas ektoparasit pada benih ikan Nila (Oreochromis niloticus) Prislawati Paputungan; Reni L. Kreckhoff; Reiny A. Tumbol; Ockstan J. Kalesaran; Juliaan Ch. Watung; Suzanne L. Undap
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to inventory the types of parasites that exist in the Center for Development and Development of Tateli Fish Farming (BP2HPI) Tateli. This research was conducted in April-July 2023 at BP2HPI, Tateli as the location for sampling 30 tilapia (Oreochromis niloticus) seeds randomly in 3 nursery ponds, and then brought to the Laboratory of Aquaculture Technology FPIK Unsrat for parasite observations. Before examining the fish parasites, the sample is put into a bucket filled with water and given aeration so that the fish are still alive during the inspection. In this research activity, several water quality parameters were also measured, namely temperature, pH, and DO in situ. The procedure for examining ectoparasites refers to the procedure according to Kabata (1985). Parasite examination begins with observing the outside of the body, and taking notes if abnormal signs are found such as swelling, bleeding, the presence of black or white spots on the surface of the body, or changes in body color, then the presence of parasites is examined using a microscope with 400x magnification. The examination found 2 types of parasites that infect the seeds of tilapia (Oreochromis niloticus) at the Center for Development and Control of Fish Pests and Diseases (BP2HPI), Tateli are Ttrichodina sp and Gyrodactylus sp. The prevalence of parasites that attack tilapia seeds as a whole reaches 100% which is divided into 90% Trichodina sp and 10%, Gyrodactylus sp. Keywords: parasitic infestation, fish disease, water quality, aquaculture
Prevalensi dan insidensi ektoparasit pada udang vaname (Litopenaeus vannamei) di tambak CV. Pasifik Delyano Farm Mokoginta, Leyda H.; Kreckhoff, Reni L.; Tumbol, Reiny A.; Longdong, Sammy N.J.; Sambali, Hariyani; Mingkid, Winda M.
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.51960

Abstract

This study aimed to inventory the parasites that attack vaname shrimp (Litopenaeus vannamei) and to determine and determine the prevalence and incidence rates of parasites that infect vaname shrimp. The research was carried out at CV. Pasifik Delyano Farm, Iyok Village, District. Bolangitang, Kab. North Mongondow. Parasite identification is carried out in the Laboratory of Aquaculture Technology FPIK Unsrat. The test animals used in this rstudy were vannamei shrimp with a weight ranging from 7-10 grams with a length of 10-15 cm. The number of shrimp samples taken was 10 in the pond. Methods for examining and observing ectoparasites were carried out on the gills, walking legs, swimming legs, and tail of vannamei shrimp and then identified to obtain prevalence and incidence rates. The observation results were then analyzed using prevalence and incidence rate formulas. Based on the research results, two types of parasites were found that infect shrimp, walking legs, swimming legs, and tail, namely Vorticella and Zootanium. Prevalence rates based on parasite type were obtained in Vorticella sp. 40% and Zoothamnium sp 30% in the category of infections that generally occur while the prevalence rate based on organs, swimming legs was higher (0.4%) than in tail (0.3%) and walking legs (0.2%). based on the level of parasite incidence in all observed shrimp organs, Zoothamnium sp. was higher than Vorticella sp.  Keywords: diseases, parasites, aquaculture, intensive cultivation
Fluktuasi parameter kualitas air di tambak super intensif udang vaname: Studi kasus pertambakan PT. Pillar Persada Parigi, Desa Bajo, Minahasa Selatan Telaumbanua, Beatus H.; Pangemanan, Novie P.L.; Tumbol, Reiny A.; Undap, Suzanne L.; Tumembouw, Sipriana S.; Salindeho, Indra R.N.
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 2 (2024): Juli - Desember
Publisher : Universitas Sam Ratulangi

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Abstract

The aim of this research was to determine the physical and chemical parameters of water quality in vanname shrimp culture in Pond PT. Pillar Persada Parigi, Bajo Village, South Minahasa. This research uses direct measurement methods in the field (In situ) and sample analysis in the laboratory. Water quality parameters measured in the field included temperature, degree of acidity (pH), salinity, dissolved oxygen, while ammonia, nitrite, nitrate, phosphate was analyzed at the Manado Industrial Research and Standardization Center. Water sampling and in-situ measurements were carried out in 4 ponds selected randomly from the ponds that were temporarily operating at the time of sampling. Based on the results of measurements of several physical and chemical parameters of water quality, both directly in the field (in situ) and the results of laboratory analysis of pond water samples from PT Pillar Persada Parigi, Bajo Village, South Minahasa Regency, a temperature range of 27 - 32˚C, salinity of 36 ppt was obtained. , dissolved oxygen 3.63 - 7.8 ppm, acidity degree (pH) 7.6 - 8.6, ammonia content 1 mg/L, nitrite <0.2757 mg/L, nitrate 5 mg/L, phosphate <0 .0027 – 1 mg/L. All parameters measured were still considered suitable for survival and supporting the growth of the vaname shrimp being cultured Keywords: vaname shrimp, dissolved oxygen, ammonia, nitrate
Good Fish Handling Techniques to Maintain the Quality of Catch from Ship to Consumer Wijaya, Haryadi; Dien, Henny Adeleida; Tumbol, Reiny A.; Mentang, Feny
Jurnal Ilmiah Platax Vol. 12 No. 2 (2024): ISSUE JULY-DECEMBER 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i2.55636

Abstract

Proper handling of fish or fishery products from ships to consumers necessitates collaboration among fishermen, government agencies, and other stakeholders. By adopting a holistic approach encompassing technical, regulatory, and educational aspects, we can ensure the efficient and sustainable operation of fisheries supply chains for all involved parties. This article presented a literature review on good fish handling practices from ship to consumer, focusing on onboard handling, auctions, landing, and transportation. Using the search term 'Good Handling Practices for Fishing Products,' six articles out of 4,470 were identified that met the criteria for further discussion. The literature review findings highlighted optimal strategies for increasing fishing, auction, and transportation processes. These included improving record-keeping and documentation, implementing aerator systems to preserve fish quality, bulk fish cooling using ice cubes without water to mitigate quality deterioration, ensuring sanitation of fish distribution support equipment to prevent bacterial contamination, and utilizing tools such as Trolley Lifters to increase the efficiency of the fish distribution process. Therefore, governmental and non-governmental institutions should provide support and training to fishermen to maximize both the quality and quantity of fish catches. Keywords: Fish Handling Techniques, Catch Quality, Ships, Consumers
Identifikasi bakteri Vibrio spp. penyebab vibriosis pada udang vanname (Litopenaeus vannamei) di PT. Pilar Persada Parigi Putra, Stevano F.P; Tumbol, Reiny A.; Undap, Suzanne L.; Kreckhoff, Reni L.; Pangemanan, Pankie N.L
e-Journal BUDIDAYA PERAIRAN Vol. 12 No. 2 (2024): Juli - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v12i2.58872

Abstract

The purpose of this study is to isolate and identify Vibrio spp. bacteria from vannamei shrimp (Litopenaeus vannamei) in PT. Pilar Persada Parigi Bajo Village, South Minahasa Regency. The research was carried out by starting with shrimp sampling from shrimp cultivation locations at PT. Persada Parigi Pillar in Bajo Village, Tatapaan, South Minahasa, North Sulawesi as many as 9 fish from 3 ponds (1 pond took 3 shrimp). The pond was recommended by the pond technician of PT. The Persada Parigi pillar is related to the condition of the pond which is suspected of having Vibrio spp. bacteria that need special attention. The shrimp samples were then taken to the Laboratory alive. During transportation, the samples were put in 3 separate containers equipped with battery aerators, then the identification of Vibrio spp. bacteria was carried out at the Laboratory of Pharmaceutical Microbiology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University and at the Manado Fishery Products Quality Control and Safety Quarantine Center. Isolation of Vibrio spp. bacteria using TCBS (Thiosulfate Citrate Bile Sucrose) agar media. The characterization of Vibrio spp. bacteria was carried out by a series of tests, namely gram staining tests (gram negative, positive and bacterial form), main tests (KOH 3%, H₂O₂ and oxidase), Oxidation/Fermentation (O/F), Glucose and Motility Indole Ornithin (MIO). The results of the isolation showed that there were 9 isolates of Vibrio spp. bacteria from 9 samples of vannamei shrimp (Litopenaeus vannamei) from 3 different ponds. The results of bacterial characteristics obtained negative grams, yellow and green colony morphology, bacil/stem cell shape, KOH 3% (-), catalase (+), oxidase (+), O/F (fermentative), indole (+), and motility (+), glucose (+) from the results identified by Vibrio spp. bacteria. Keywords: Litopenaeus vannamei, Vibrio spp., Vibriosis, isolation, identification