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All Journal Biopropal Industri BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia Makara Journal of Science Makara Journal of Technology Jurnal Mahasiswa Mengabdi (JIMAWAbdi) JUPADAI : Jurnal Pengabdian Kepada Masyarakat Jurnal Manajerial dan Bisnis Tanjungpinang Journal Of Chemical Process Engineering Annales Bogorienses
YOPI YOPI
Lembaga Ilmu Pengetahuan Indonesia
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Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Thontowi, Ahmad; Rahmani, Nanik; Yopi, Yopi
Annales Bogorienses Vol. 17 No. 1 (2013): Annales Bogorienses
Publisher : BRIN

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Bto5 is marine bacterium
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

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Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy Thenawidjaja; Meryandini, Anja; Prasetya, Bambang
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
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The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.
Glucoamylase Production by Aspergillus awamori KT-11 In Solid State Fermentation Using Cassava Peel as Substrate Perwitasari, Urip; Nuryati, Nuryati; Melliawati, Ruth; Yopi, Yopi
Annales Bogorienses Vol. 21 No. 1 (2017): Annales Bogorienses
Publisher : BRIN

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Cassava has long been known as one of the main staple food in Indonesia. Whereas the cassava peel contains starch of approximately 72%, it is still underrated as a carbohydrate source for fermentation.The utilization of cassava peel as a substrate in solid state fermentation potentially replaces rice as a carbon source leading to more cost-effective production. This study aims at producing glucoamylase by means of solid state fermentation using Aspergillus awamori KT-11 and cassava peel as substrate. The study demonstrated that medium composition and drying technique affected the production of glucoamylase. The highest glucoamylase activities were identified when cassava peel and mineral media was used in fermentation, compared to only cassava peel; the combination of cassava peel, mineral, and rice bran; rice media or a mixture of rice, mineral and rice bran. Freeze-dried glucoamylase, furthermore, exhibited higher specific activity in contrast to the oven-dried one, with 452 U/mL and 365 U/mL, respectively. In conclusion, cassava peel plus mineral is a better substrate for glucosamine production by A. awamori KT-11 in solid state fermentation. Besides, powdered glucoamylase had been demonstrated to be capable of hydrolyzing starch-based biomass.
Co-Authors A.A. Ketut Agung Cahyawan W ADE ANDRIANI Ahmad Thontowi Anja Meryandini Awan Purnawan Bambang Prasetya Budiawan Budiawan Dewi, Fitria DWI SUSILANINGSIH Elvi Yetti, Elvi Endang Sukara Gulo, Happy Christman Hapzi Ali Harayama, Shigeaki Harayama, Shigeaki Harwati, Theresia Umi Helprida Simamora Hidayat, Haris Suganda Ilmiah, Zidny Jonmaianto Sihombing La Ifa Maggy T Suhartono Maggy Thenawidjaja Suhartono Muhammad Iqbal NANIK RAHMANI Nuryana, Isa Nuryati Nuryati Nuzul Farini, Nuzul Okazaki, Fumiyoshi Okazaki, Fumiyoshi Oktavianti Oktavianti Palupi, Nurheni Sri Purwoko, Agus PUSPITA LISDIYANTI Putra, Filemon Jalu Nusantara Rahmasari, Dianti Rahmasari, Dianti Rohmattusolihat Rohmattusolihat, Rohmattusolihat ROHMATUSSOLIHAT ROHMATUSSOLIHAT Ruth Melliawati Safitriani, Safitriani Safitriani, Safitriani Sari, Yana Nurita Sari, Yana Nurita Septa Diana Nabella SHANTI RATNAKOMALA Siswahyudianto Sita Heris Anita Sri Hartati Sri Pujiyanto Suhanda La Tima, Suhanda Sulastri Sulastri Suryani Suryani Susiana Purwantisari Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Theresia Harwati, Theresia Tun Tedja Irawadi Tutik Murniasih Urip Perwitasari, Urip Wahyudi, Muhammad Eko WIBOWO MANGUNWARDOYO Wijanarka Wijanarka Wijaya, Hans Yantyati Widyastuti