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All Journal BALI MEDICAL JOURNAL (BMJ) Medicina E-Jurnal Medika Udayana E-Jurnal Udayana Medica WMJ (Warmadewa Medical Journal) JNKI (Jurnal Ners dan Kebidanan Indonesia) (Indonesian Journal of Nursing and Midwifery) Indonesian Journal of Obstetrics & Gynecology Science ISM (Intisari Sains Medis) : Jurnal Kedokteran Jurnal Ilmiah Sangkareang Mataram Indonesian Journal of Perinatology Public Health and Preventive Medicine Archive Journal Research of Social Science, Economics, and Management Journal of Global Pharma Technology
I Nyoman Hariyasa Sanjaya
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : BALI MEDICAL JOURNAL (BMJ)

 1 
GROUP-B STREPTOCOCCUS IN PREGNANT WOMEN: Prevalence of Colonization and Sensitivity Pattern in Denpasar during June 2007?May 2008 Sri-Budayanti,, N; Hariyasa-Sanjaya, and, N
BALI MEDICAL JOURNAL Volume 2 Number 1, January-April 2013
Publisher : BALI MEDICAL JOURNAL

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Objective: Group-B Streptococci (GBS) are Gram-positive cocci that are the most common cause of early onset neonatal sepsis. The mortality rate of early onset neonatal sepsis has been reported up to 50%. One of the major risks of early onset neonatal sepsis is GBS colonization in birth canal of pregnant women that can infect the baby during process of vaginal delivery. Antibiotic chemoprophylaxis for pregnant women that is colonized by GBS can reduce the risk of early onset neonatal sepsis. The detection of GBS colonization needs Todd Hewitt (TH) enrichment medium to reduce false negative result. Until now, there is no report about either prevalence of colonization or sensitivity pattern of Group B Streptococcus among pregnant women in Denpasar. The aims of this research were to determine the prevalence of GBS colonization and sensitivity pattern of GBS among pregnant women with Todd Hewitt enrichment medium. Method: This research was a descriptive cross-sectional study. Vaginal swab specimens from 35-37 weeks gestation pregnant women were collected and 32 samples that met the inclusion criteria were cultured on Blood agar (BA) plates, Chromagar (CA) plates, and Todd Hewitt (TH) broth. The GBS colonization that grew in culture medium was followed by antibiotic sensitivity test. Results: In the present study, we found that the prevalence of GBS colonization in pregnant women detected with culture method using BA and CA without TH broth was 9.4%, whereas the prevalence with culture method using BA and CA enriched by TH broth was 31.3%. Moreover, GBS showed resistance to penicillin, erythromycin, and cefazolin. It is indicated that TH enrichment medium seems to be promising as a screening method for GBS colonization in pregnant women in Bali. Conclusion: There was an enrichment detection of GBS prevalence colonization in pregnant women detected the swab with culture method using BA and CA enriched by TH compare to BA and CA without TH broth. Moreover, GBS showed resistance to penicillin, erythromycin, and cefazolin. It is indicated that TH enrichment medium seems to be promising as a screening method for GBS colonization in pregnant women in Bali.
Lower Serum Catalase Level is Associated with Preterm Labor among Pregnant Women at Sanglah Hospital Denpasar, Bali-Indonesia Negara, I Ketut Surya; Surya, IGP; Sanjaya, Hariyasa; Anantasika, AAN; Mahardika, I Made
BALI MEDICAL JOURNAL Vol 5 No 3 (2016)
Publisher : BALI MEDICAL JOURNAL

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Background: Preterm labor is still become a serious health problem in Obstetric and Perinatology with no sensitive biomarker currently approved. Several studies show that decrease antioxidant activity may play significant role in preterm labor. However, only few studies had been conducted to evaluate blood catalase level in preterm labor and assess its role in preterm labor. Objective: The aim this study was to identify the differences of maternal serum catalase level in preterm labor compared with preterm pregnancy. Methods: An observational analytic cross sectional study was conducted from February to December 2014 using pregnant women with 28-36 weeks’ gestational age. Blood catalase level was evaluated by colorimetric method and the data was analyzed by SPPS for Windows 17.0 program. Results: 12 subjects were enrolled and divided into preterm and control group. No significant differences between mean age, gestational age, and parity between preterm and control group. However, blood catalase level was significantly lower in preterm group compared with control group (81.82 ± 20.38 vs 159.38 ± 35.79; p=0.001). Conclusion: Serum maternal catalase level were significantly lower in preterm labor compared with preterm normal pregnancy.
Fetal Sex Determination Using Cell-Free Fetal Dna (cffDNA) in Maternal Blood Sanjaya, I Nyoman Hariyasa; Suwardewa, Tjok Gde Agung; Arijana, I G. Kamasan N.
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

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Background: Prenatal test has routinely performed in antenatal care and has become a part of the obstetric care feature in many countries. Prenatal test is divided into screening and diagnostic test. Recently, the early noninvasive method in order to found and lessen the risk factors of pregnancy loss, has been studied. One of the methods is molecular test using cffDNA which has many screening purpose such as sex determination, aneuploidy, paternal inherited genetic disorder, fetus rhesus, and performed early at 7 weeks of pregnancy. Objective: The purpose of this study is to measure diagnostic value of cffDNA in determining fetal sex prenatally. Methods: In a diagnostic test study, 18 randomized samples were selected and divided based on fetal gender confirmed at birth. The group consisted of 9 pregnant women with male babies and 9 pregnant women with female babies. CffDNA then isolated from maternal blood sample and specific region in Y chromosome termed SRY is detected by PCR and electrophoresis. The data obtained analyzed both descriptively for baseline characteristic and analytically to determine its diagnostic value. Results: This study found significant correlation between SRY detection in cffDNA with male fetal phenotype (p<0.05). The sensitivity of the method is 100% with 89% specificity. In addition, we found 9.09 values for positive likelihood ratio (LR+) and 0 for negative likelihood ratio (LR-). Moreover, the result yielded 100% positive predictive value (PPV+) and 88.8% of negative predictive value (PPV-). Conclusion: This study proofed that cffDNA have a great diagnostic value to determine fetal sex prenatally. However, further study with several group of gestational age mother and better matching is required to further confirm the diagnostic potential of cffDNA.
Co-Authors Adhi Pribadi Aldiansyah, Dudy Aldika Akbar, Muhammad Ilham Aloysius Suryawan Anak Agung Ngurah Anantasika Anak Agung Wahyu Putri Agustini Anak Agung Wahyu Putri Agustini Anak Agung Wahyu Putri Agustini Anak Agung Wahyu Putri Agustini Andonotopo, Wiku Bachnas, Muhammad Adrianes Bambang Rahardjo Cokorda Istri Mirayani Pemayun Cokorda Istri Mirayani Pemayun Cokorda Istri Mirayani Pemayun Cokorda Istri Mirayani Pemayun Cut Meurah Yeni Daniel H. Susanto Denni Prasetyo Dyah Pradnyaparmita Duarsa Eka Setiawan, Putu Pandu Endang Sri Widiyanti Evert Solomon Pangkahila Firsta Sesarina Mintariani Firsta Sesarina Mintariani Firsta Sesarina Mintariani Hartanto Hartanto I G A A Novya Dewi I Gede Ngurah Harry Wijaya Surya I Gusti Kamasan Arijana I Ketut Surya Negara I Made Gede Widnyana I Made Mahardika I Nyoman Gede Budiana I Nyoman Wirata I Wayan Murdita Ida Bagus Gde Fajar Manuaba Ida Bagus Putra Adnyana Ida Bagus Putra Adnyana, Ida Bagus IGP Surya, IGP Julian Dewantiningrum Kadek Budi Juliantari Kamajaya, I Gusti Ngurah Agung Trisnu Ketut Widyani Astuti Kurjak, Asim Kusuma, Anak Agung Ngurah Jaya luh sudiarmini Made Adi Kusuma Made Diah Vendita Sakuntari Made Diah Vendita Sakuntari Made Diah Vendita Sakuntari Made Wiryana Marta, Kadek Fajar Murdita, I Wayan Ni Kadek Mulyantari Ni Komang Anik Pirgantari Ni Komang Anik Pirgantari Ni Komang Anik Pirgantari Ni Komang Erny Astiti Ni Luh Made Diah Mas Cahyani Putri Ni Luh Made Diah Mas Cahyani Putri Ni Luh Made Diah Mas Cahyani Putri Ni Luh Md Dwi Laxmi Satriani Ni Luh Md Dwi Laxmi Satriani Ni Luh Md Dwi Laxmi Satriani Ni Luh Putu Yulia Padmawati Ni Nyoman Dewi Purwanti Ni Nyoman Sri Budayanti Ni Putu Nining Gianni Ni Putu Nining Gianni Ni Putu Nining Gianni Ni Wayan Dewi Purwanti Ni Wayan Dewi Purwanti Ni Wayan Dewi Purwanti Ni Wayan Dewi Purwanti Nuswil Bernolian Pramono, Mochammad Besari Adi Putra, Wayan Artana Putu Doster Mahayasa Putu Pandu Eka Setiawan Rey Jauwerissa Ryan S. Mulyana Ryan Saktika Mulyana Sri Sulistyowati Stanojevic, Milan Sutandi, Chatrine Syauta, Fetrisya Tasa Riszkia Theresia Monica Rahardjo Tjokorda Gde Agung Suwardewa Utarini, Gusti Ayu Eka Widi, Made Yudha Ganesa Wikantyas Wijaya Surya, I Gede Ngurah Harry Wiradnyana, Anak Agung Gede Putra Wiradnyana, Anak Agung Putra