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The Impact of Artificial Barriers on the Varuna litterata Migration Route in the Lower Serayu River, Central Java and Its Molecular Identification Sinaga, Sorbakti; Maimunah, Yunita; Ekawati, Arning Wilujeng; Widodo, Maheno Sri
Journal Omni-Akuatika Vol 20, No 1 (2024): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2024.20.1.1140

Abstract

The Serayu River is one of the longest rivers in Central Java. Dams built in the lower reaches of the Serayu River are thought to block the migration paths of fish and aquatic macrocrustaceans. This study aimed to determine the effect of dam on Varuna litterata migration and its molecular identification. This research was conducted using the observation method. Migration observations were made on vertical paths while molecular identification of V. litterata in the lower reaches of the Serayu River was compared with existing data in GenBank. The study covered the freshwater area above the Serayu Dam, the open area with freshwater below the Serayu Dam, and the open area with brackish water at the estuary of the Serayu River, with a study length of 33.37 km. The results showed that the presence of dams in the lower reaches of the Serayu River inhibited the migration pattern of V. litterata. The vertical shape of the dam causes only a few V. litterata to migrate to the top of the dam. The dam in the lower reaches of the Serayu River resulted in not many V. litterata being able to migrate to the top of the dam, so there were very few V. litterata found in the population upstream of the dam (115 ind). The results of molecular identification of V. litratata using the cytochrome C oxidase subunit I (COI) gene show that this crab is 100% the same as the data in GenBank and is closely related to Varuna yui and other Varunidae crab species.Keywords: Dams, Molecular, Migration, Serayu River, Varuna litterata
The comparison of Phytochemical Composition, Total Polysaccharides, and Highest Nutrient Content in Seaweed (Ulva lactuca) from Two Different Locations Lailaturramadhini, Nova; Yuniarti, Ating; Maimunah, Yunita; Eka Supriatin, Febriyani; Suryanto, Damang; Ridwanudin, Asep
Journal of Aquaculture and Fish Health Vol. 14 No. 2 (2025): JAFH Vol. 14 No. 2 June 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i2.70265

Abstract

Ulva lactuca is a source of structurally diverse and highly valuable bioactive compounds. Its chemical composition includes carbohydrates (up to 60%), proteins (10% - 47%), lipids (1% - 3%), and bioactive compounds such as polyphenols, which exhibit antibacterial, antiviral, antioxidant, and immunomodulatory properties. Ulvan, a heteropolysaccharide present in its cell walls, is known for its antiviral, antioxidant, and antimicrobial properties.  This study aims to identify the potential of Ulva lactuca as a source of nutrition and health benefits. The research is descriptive-exploratory, aiming to analyze the phytochemical composition and total polysaccharides in Ulva lactuca from Ujung Genteng Beach, Sukabumi, West Java, and Lombok Beach, West Nusa Tenggara. The results indicate that the highest flavonoid content was found in the Ulva lactuca extract from Sukabumi, measuring 3.95 mg/L, compared to 3.686 mg/L in Ulva lactuca from Lombok. The alkaloid content in Ulva lactuca from Lombok was 26.53 mg/L, while in Ulva lactuca from Sukabumi, it was 24.78 mg/L. The highest tannin content was found in Ulva lactuca from Lombok at 9.87 mg/L, compared to 3.53 mg/L in Ulva lactuca from Sukabumi. The polysaccharide content in Ulva lactuca from Sukabumi was 50.83 mg/L, whereas it was 38.165 mg/L in Ulva lactuca from Lombok. Proximate analysis showed that Ulva lactuca from Lombok had a higher protein content (20.44%) compared to that from Sukabumi (15.47%).
Community Empowerment Strategy of Pondok Agung, Kasembon Sub-district through Biogas Technology to Reduce Cattle Waste Pollution in Water Maimunah, Yunita; Kilawati, Yuni; Muttaqin, Adharul; Amrillah, Attabik Mukhammad; Kartikasari, Dany Primanita
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.9544

Abstract

The community-based biogas project in Kasembon sub-district focuses on utilising livestock manure as a renewable energy source and organic fertiliser producer to improve community welfare and reduce negative environmental impacts. Through an Asset-Based Community Development (ABCD) approach, the project identifies local assets, such as land and manure, and empowers the local community to maintain the biogas system. The biogas production process produces environmentally friendly energy and reduces dependence on LPG. In addition, the organic fertiliser made from biogas waste can be utilised in agriculture to improve productivity and soil health. The implementation of ABCD encourages collaboration between the community, government, and university, and creates new economic opportunities by selling organic fertiliser. However, challenges such as dependence on external assistance, high initial capital requirements, and technical limitations hinder long-term sustainability.
Penggunaan Ekstrak Daun Lamtoro (Leucaena Leucocephala) Sebagai Pupuk Dengan Salinitas Yang Berbeda Terhadap Laju Pertumbuhan, Biomassa Dan Klorofil-A Pada Mikroalga Chlorella Vulgaris Aulia, Ana Evita; Maimunah, Yunita; Suprastyani, Heny
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 1 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.8

Abstract

Kultur Chlorellah vulgaris umumnya menggunakan pupuk Pro Analisis (PA), mahalnya harga pupuk PA menjadi dasar pencarian pupuk alternatif yang mampu memenuhi kebutuhan nutrisi C. vulgaris. Pupuk alternatif tersebut yaitu pupuk organik berbahan baku daun lamtoro (L. Leucocephala) yang diekstrak. Tujuan dari penelitian ini yaitu mengetahui mengetahui pengaruh perbedaan salinitas dan mengetahui nilai salinitas yang optimal pada pupuk ekstrak daun lamtoro untuk laju pertumbuhan, biomassa dan klorofil-a C.  vulgaris. Metode yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan. Perlakuan yang diberikan pada penelitian ini adalah pupuk ekstrak daun lamtoro (L. Leucocephala) dosis 1,75 ml/l dengan salinitas 30 ppt, 35 ppt, 40 ppt dan kontrol (walne 1 ml/l) salinitas 30 ppt. Parameter utama yang diamati yaitu pertumbuhan, biomassa dan klorofil-a C. vulgaris. Hasil penelitian menunjukkan bahwa pemberian pupuk ekstrak daun lamtoro (L. Leucocephala) dengan salinitas yang berbeda berpengaruh nyata terhadap pertumbuhan, biomassa dan klorofil-a C. vulgaris. Perlakuan terbaik selama penelitian didapatkan hasil laju pertumbuhan spesifik 1,94/hari pada salinitas 35 ppt, biomassa sebesar 0,403 g/l pada salinitas 35 ppt dan klorofil-a sebesar 0,018 µg/ml pada salinitas 35 ppt. Berdasarkan hasil penelitian ini, disarankan agar menggunakan salinitas 34 untuk penelitian lanjutan sesuai dengan hasil maksimum agar mendapatkan hasil yang lebih baik.
In Silico Study of Betaine, Isoleucine, and DL-Stachydrine Compounds in Shipworm (Spathoteredo obtusa) Extract as an Antibacterial Agent of Aeromonas hydrophila Andi Al Furqan; Fadjar, Mohamad; Maimunah, Yunita
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.72940

Abstract

The rapid increase in freshwater commodity production is accompanied by various obstacles that pose challenges for farmers, namely, disease infections. Bacterial infection by Aeromonas hydrophila is the pathogenic agent causing Motile Aeromonas Septicemia (MAS), which can result in 100% mortality within a short period. The AhlC toxin protein in A. hydrophila bacteria plays the most critical role in the Ahl tripartite toxin, as AhIC acts as a protomer and inserts itself into one membrane layer, then binds to AhlB and AhlA to form pores in both membrane layers. Active compounds found in marine mussel extracts (Spathoteredo obtusa), particularly betaine, isoleucine, and DL-stachydrine, have the potential to inhibit the AhlC toxin protein produced by A. hydrophila bacteria. This study aims to predict the interaction between the AhlC receptor protein in A. hydrophila bacteria and the active compounds identified from the extract of shipworms (S. obtusa) using molecular docking methods. The test results showed that all three compounds met all ADME predictions, with the best binding affinity value of -4.2 kcal/mol for isoleucine and DL-stachydrine, followed by -3.5 kcal/mol for betaine. Based on the test results, there are appropriate, stable, and effective hydrogen and electrostatic charge interactions with the ligand-receptor complex (ASN:32, GLN:35, ARG:112, and ASP:116), which play a crucial role as active sites in ligand binding to the receptor.
Identification of Chemical Compounds of Guava Leaf Fractions using Phytochemical Test, UV-Vis Spectrophotometry and GC-MS Wardani, Lailatul Munawaroh Dewi Kusuma; Andayani, Sri; Maimunah, Yunita
JST (Jurnal Sains dan Teknologi) Vol. 14 No. 1 (2025): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v14i1.94481

Abstract

Fraction is the result of fractionation of the process of separating compounds based on the polarity properties of the solvent. Thin layer chromatography is the first step in fractionation analysis to determine the eluent that can produce guava leaf fractions. The guava leaf fraction contains chemical compounds that play a role in improving the immune system. Guava leaves contain flavonoid compounds, especially quercetin. The effort made to find out the content of guava leaves is to analyze further. Some analyses to determine the content of active compounds, especially in guava leaf fractions, are using phytochemical tests, UV-VIS spectrophotometry, and GC-MS. The combination of these three methods can obtain comprehensive information about the content of chemical compounds in plants, from the identification of compound groups to the characteristics of specific compounds. Phytochemical analysis is an initial analytical method carried out to examine the content of chemical compounds in medicinal plants. Ultra Violet-Visble (UV-Vis) spectrophotometer is one of the chemical analysis methods to determine the composition of a sample, both quantitatively and qualitatively, based on the interaction between matter and light. The Gas Cromatography-Mass Spectrometry (GC-MS) method is a method of separating samples using gas chromatography while analyzing the compounds using mass spectroscopy. The purpose of this study is to analyze the active compounds contained in the guava leaf fraction. This research uses a qualitative method by collecting data by observation and literature study.  The results showed that the results of the analysis of guava leaf fractions using phytochemical tests, UV-VIS spectrophotometers, and GC-MS, showed that the fraction contained terpenoid and flavonoid group compounds.