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Penggunaan Ekstrak Daun Lamtoro (Leucaena Leucocephala) Sebagai Pupuk Dengan Salinitas Yang Berbeda Terhadap Laju Pertumbuhan, Biomassa Dan Klorofil-A Pada Mikroalga Chlorella Vulgaris Aulia, Ana Evita; Maimunah, Yunita; Suprastyani, Heny
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 1 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.8

Abstract

Kultur Chlorellah vulgaris umumnya menggunakan pupuk Pro Analisis (PA), mahalnya harga pupuk PA menjadi dasar pencarian pupuk alternatif yang mampu memenuhi kebutuhan nutrisi C. vulgaris. Pupuk alternatif tersebut yaitu pupuk organik berbahan baku daun lamtoro (L. Leucocephala) yang diekstrak. Tujuan dari penelitian ini yaitu mengetahui mengetahui pengaruh perbedaan salinitas dan mengetahui nilai salinitas yang optimal pada pupuk ekstrak daun lamtoro untuk laju pertumbuhan, biomassa dan klorofil-a C.  vulgaris. Metode yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan empat ulangan. Perlakuan yang diberikan pada penelitian ini adalah pupuk ekstrak daun lamtoro (L. Leucocephala) dosis 1,75 ml/l dengan salinitas 30 ppt, 35 ppt, 40 ppt dan kontrol (walne 1 ml/l) salinitas 30 ppt. Parameter utama yang diamati yaitu pertumbuhan, biomassa dan klorofil-a C. vulgaris. Hasil penelitian menunjukkan bahwa pemberian pupuk ekstrak daun lamtoro (L. Leucocephala) dengan salinitas yang berbeda berpengaruh nyata terhadap pertumbuhan, biomassa dan klorofil-a C. vulgaris. Perlakuan terbaik selama penelitian didapatkan hasil laju pertumbuhan spesifik 1,94/hari pada salinitas 35 ppt, biomassa sebesar 0,403 g/l pada salinitas 35 ppt dan klorofil-a sebesar 0,018 µg/ml pada salinitas 35 ppt. Berdasarkan hasil penelitian ini, disarankan agar menggunakan salinitas 34 untuk penelitian lanjutan sesuai dengan hasil maksimum agar mendapatkan hasil yang lebih baik.
In Silico Study of Betaine, Isoleucine, and DL-Stachydrine Compounds in Shipworm (Spathoteredo obtusa) Extract as an Antibacterial Agent of Aeromonas hydrophila Andi Al Furqan; Fadjar, Mohamad; Maimunah, Yunita
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.72940

Abstract

The rapid increase in freshwater commodity production is accompanied by various obstacles that pose challenges for farmers, namely, disease infections. Bacterial infection by Aeromonas hydrophila is the pathogenic agent causing Motile Aeromonas Septicemia (MAS), which can result in 100% mortality within a short period. The AhlC toxin protein in A. hydrophila bacteria plays the most critical role in the Ahl tripartite toxin, as AhIC acts as a protomer and inserts itself into one membrane layer, then binds to AhlB and AhlA to form pores in both membrane layers. Active compounds found in marine mussel extracts (Spathoteredo obtusa), particularly betaine, isoleucine, and DL-stachydrine, have the potential to inhibit the AhlC toxin protein produced by A. hydrophila bacteria. This study aims to predict the interaction between the AhlC receptor protein in A. hydrophila bacteria and the active compounds identified from the extract of shipworms (S. obtusa) using molecular docking methods. The test results showed that all three compounds met all ADME predictions, with the best binding affinity value of -4.2 kcal/mol for isoleucine and DL-stachydrine, followed by -3.5 kcal/mol for betaine. Based on the test results, there are appropriate, stable, and effective hydrogen and electrostatic charge interactions with the ligand-receptor complex (ASN:32, GLN:35, ARG:112, and ASP:116), which play a crucial role as active sites in ligand binding to the receptor.
Identification of Chemical Compounds of Guava Leaf Fractions using Phytochemical Test, UV-Vis Spectrophotometry and GC-MS Wardani, Lailatul Munawaroh Dewi Kusuma; Andayani, Sri; Maimunah, Yunita
JST (Jurnal Sains dan Teknologi) Vol. 14 No. 1 (2025): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v14i1.94481

Abstract

Fraction is the result of fractionation of the process of separating compounds based on the polarity properties of the solvent. Thin layer chromatography is the first step in fractionation analysis to determine the eluent that can produce guava leaf fractions. The guava leaf fraction contains chemical compounds that play a role in improving the immune system. Guava leaves contain flavonoid compounds, especially quercetin. The effort made to find out the content of guava leaves is to analyze further. Some analyses to determine the content of active compounds, especially in guava leaf fractions, are using phytochemical tests, UV-VIS spectrophotometry, and GC-MS. The combination of these three methods can obtain comprehensive information about the content of chemical compounds in plants, from the identification of compound groups to the characteristics of specific compounds. Phytochemical analysis is an initial analytical method carried out to examine the content of chemical compounds in medicinal plants. Ultra Violet-Visble (UV-Vis) spectrophotometer is one of the chemical analysis methods to determine the composition of a sample, both quantitatively and qualitatively, based on the interaction between matter and light. The Gas Cromatography-Mass Spectrometry (GC-MS) method is a method of separating samples using gas chromatography while analyzing the compounds using mass spectroscopy. The purpose of this study is to analyze the active compounds contained in the guava leaf fraction. This research uses a qualitative method by collecting data by observation and literature study.  The results showed that the results of the analysis of guava leaf fractions using phytochemical tests, UV-VIS spectrophotometers, and GC-MS, showed that the fraction contained terpenoid and flavonoid group compounds.
HUBUNGAN KELIMPAHAN MIKROPLASTIK DENGAN KERUSAKAN HISTOPATOLOGIS PADA INSANG DAN USUS UDANG VANAME (Litopenaeus vannamei) YANG DIBUDIDAYAKAN DI TAMBAK DI PROBOLINGGO, JAWA TIMUR, INDONESIA Putra, Andhika Farras Rahardian; Hertika, Asus Maizar Suryanto; Maimunah, Yunita
Jurnal Riset Akuakultur Vol 20, No 1 (2025): Maret (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.1.2025.63-77

Abstract

Peningkatan kontaminasi mikroplastik di lingkungan tambak menjadi ancaman serius bagi kesehatan udang vaname (Litopenaeus vannamei). Penelitian ini bertujuan untuk menganalisis hubungan antara kelimpahan mikroplastik pada insang dan usus dengan tingkat kerusakan histopatologis udang vaname yang dibudidayakan di tambak yang berlokasi di Probolinggo, Jawa Timur, Indonesia. Penelitian dilakukan secara deskriptif kuantitatif menggunakan analisis korelasi Spearman. Sampel udang diambil dari tiga lokasi tambak dan dianalisis secara histologis untuk menilai skor kerusakan jaringan. Hasil menunjukkan korelasi sangat kuat dan signifikan antara kelimpahan mikroplastik dengan skor kerusakan insang (r = 0,815; p-value = 0,007), degenerasi usus (r = 0,885; p-value = 0,002), nekrosis (r = 0,804; p-value = 0,009), dan inflamasi (r = 0,688; p-value = 0,041). Temuan ini menunjukkan bahwa mikroplastik berkontribusi besar terhadap kerusakan struktural organ respirasi dan pencernaan udang. Penelitian ini menegaskan pentingnya pengelolaan pencemaran mikroplastik dalam sistem budidaya berkelanjutan. The increasing presence of microplastic contamination in shrimp ponds poses a serious threat to the health of whiteleg shrimp (Litopenaeus vannamei). This study aimed to analyze the relationship between microplastic abundance in gills and intestines with the level of histopathological damage in whiteleg shrimp cultivated in ponds located in Probolinggo, East Java, Indonesia. A quantitative descriptive method was used with Spearman correlation analysis. Shrimp samples were collected from three pond locations and histologically examined to assess tissue damage scores. The results showed a very strong and significant correlation between microplastics abundance and gill damage (r = 0.815; p-value = 0.007), intestinal degeneration (r = 0.885; p-value = 0.002), necrosis (r = 0.804; p-value = 0.009), and inflammation (r = 0.688; p-value = 0.041). These findings indicated that microplastics significantly contribute to structural damage in the respiratory and digestive organs of shrimp. This study highlighted the importance of effectively managing microplastic pollution in sustainable aquaculture systems.
New Records of the Egg Development Phase of Varuna litterata in the Lower Serayu River, Central Java sinaga, sorbakti; Widodo , Maheno Sri; Maimunah, Yunita
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.54757

Abstract

One of the Brachyura crabs found in the lower reaches of the Serayu River is Varuna litterata, also known as the "herring bow crab". This crab usually inhabits shallow tidal areas and hides under rocks, logs, or dead leaves. It is a highly adapted crab found in a wide range of salinities. V. litterata is also a fishery product consumed in Thailand, the Philippines, and Indonesia. This research was conducted in the Lower Serayu River, Central Java, which consists of three observation stations, namely: 1) the first station is located above the dam with fresh water, 2) the second station is below the dam with fresh water properties and 3) the third station is downstream (estuary) of the Serayu River with brackish water. This research was conducted for four months, from October 2023 to January 2024. This study aimed to determine the level of egg development in V. litterata. The results showed that V. litterata experienced four stages in egg development and then hatched into zoea. At the initial development stage, V. litterata eggs will be purple; in the second stage, the eggs will turn reddish; in the third development stage, the eggs will turn orange to brownish; and in the fourth stage, the eggs will develop and become black. V. litterata that will spawn will migrate to brackish waters to hatch their eggs. V. litterata produces 20,708 - 85,886 eggs with an average egg diameter of 0.440 - 0.466 mm.
Pemanfaatan Gracillaria sp. sebagai Agen Biofilter pada Udang Vaname (Litopenaeus vannamei) yang Dibudidayakan di Laboratorium Maimunah, Yunita; Kurniaty, Vida; Sanoesi, Ellana
Jurnal Sumberdaya Akuatik Indopasifik Vol 7 No 4 (2023): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2023.Vol.7.No.4.359

Abstract

Shrimp feed contains nitrogen and phosphorus, which are essential nutrients for shrimp growth. However, not all of the feed is consumed by the shrimp, and some of it becomes waste that accumulates in the water. There are various methods to reduce ammonia and phosphate, one of it is by bioremediation. One promising biofilter material for shrimp farming is Gracillaria sp that can absorb ammonia and phosphate from the water. Gracillaria sp. can also produce agar, a valuable substance for various industries. This research uses Gracillaria sp with different weights for bioremediation of vannamei shrimp farming effluent. Method used was experimental in Laboratory with 4 treatments, there are K: Gracillaria sp weight of 100 grams (control without shrimp), A : Gracillaria sp weight of 1 300 grams, B: Gracillaria sp. weight of of 450 grams and C: Gracillaria sp weight of 600 grams. The results showed that Gracillaria sp. can effectively reduce the levels of ammonia and phosphate in water, with biofilter efficiency reaching up to 92% for phosphate and 64% for ammonia. The higher the weight of Gracillaria sp. used in the biofilter, the more efficient it was at removing ammonia and phosphate. Additionally, Gracillaria sp. can also produce agar, a valuable substance for various industries.
Analysis of Substrate, Gastric Content and Nutrient Content of Varuna litterata Sinaga, Sorbakti; Widodo , Maheno Sri; Maimunah, Yunita
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7691

Abstract

Varuna litterata is a secondary freshwater crab found in tidal areas. This crab is caught, its eggs are taken, and it is considered a delicacy. Continuous fishing without any cultivation efforts can cause a decline in the population, leading to extinction. This study aims to determine the characteristics of the habitat substrate, stomach, and nutritional content of V. litterata. The method used in this study is descriptive exploratory. The study was conducted in the Serayu River for four months (October 2023 - January 2024). The observation location was carried out at three stations. The study showed that V. litterata prefers waters with clay sand substrates (75% sand, 25% dust, and 0% clay). This habitat is very suitable for cultivation development, as shown by the population of captured crabs. The results of observations of stomach contents show that this crab is an opportunistic omnivore. This crab has a high nutritional content with an average water content (60.72%), ash (4.57%), fat (0.52%), protein (25.66%), and carbohydrates (8.52%). Further research is essential to understand the reproductive system of V. litterata, its life cycle, and suitable habitats for spawning.
Crustacean Hyperglycemic Hormone and Gonad Histology of Female Spiny Lobster (Panulirus homarus) Inducted by Laserpuncture Wibowo, Jonathan Christian; Fadjar, Mohamad; Maimunah, Yunita
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7926

Abstract

Spiny lobster is one of the potential commodities in Indonesia. On the other hand, there is still a problem to fulfill the export-ready stock of spiny lobster. Production engineering can be utilized as one way to overcome the existing problems. Laserpuncture is a new method of production engineering by utilizing low wavelength helium-neon light that can provide biological stimulation to living things resulting in accelerated development. Laserpuncture induction in crustaceans such as lobsters can be done on the eye stalk and ventral part. This study was conducted using completely randomized design method with 4 treatments and 5 repetitions of each treatment. The treatments given are differences in the duration of laserpuncture induction on female spiny lobster including A (2 seconds induction), B (4 seconds induction), C (6 seconds induction), and control (without induction). This study aims to analyze wheter laserpuncture affects Crustacean Hyperglycemic Hormone. CHH and gonad in female spiny lobster. Data analysis in this study was carried out qualitatively which included Crustacean Hyperglycemic Hormone A & B hormone, gonad histology, and survival rate. The results showed that treatment A (2 seconds induction of laserpuncture) on female spinny lobster had the most significant effect compared to other treatments.