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Ani Suryani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Microbiology Indonesia

 1 
Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation SARA GUSTIA WIBOWO; IS HELIANTI; ANI SURYANI; BUDIASIH WAHYUNTARI
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.837 KB) | DOI: 10.5454/mi.10.3.5

Abstract

A two level factorial design was performed to optimized xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X), 1 nitrogen source (X) concentration, and pH (XCorn cob and fish powder were use as carbon and nitrogen source 2 3 respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production.  A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X) and pH (X).  The optimum corn cobs concentration was 4.37% (w/v), 1 3 fish powder P concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity.
Isolation, Characterization, and Production of Lipase from Indigenous Fungal for Enzymatic Interesterification Process LISA PRATAMA; IS HELIANTI; ANI SURYANI; BUDIASIH WAHYUNTARI
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.463 KB) | DOI: 10.5454/mi.11.2.1

Abstract

Lipase catalyses hydrolysis and esterification of lipids. The purpose of this research was to  obtain lipase producing indigenous fungi, to identify the selected fungi, to study optimum temperature and pH of the enzyme activity, as well as the  enzyme ability in interesterification reaction. The isolates used in the experiment were isolated from tempeh, oncom and BPPT laboratory culture collection. The results showed that three fungal isolates which isolated, tempe and oncom and  an isolate of BPPT-CC were positive produced lipase after qualitative assay using Rhodamine B, olive oil and PVA. The morphology identification of the isolates, revealed that R isolate was  Aspergillus sp, T isolate was Neurospora sp. and O isolate was Rhizopus sp. Upon quantitative assay from determination of the media and time production, potato dextro broth (PDB) with olive oil 2% in 48 hours fermentation showed the highest specific activity of the enzymes. Lipase produced from three isolate have the optimum at pH 4, temperatures at 40-45 °C and stable in interesterification reaction (55 °C) for 30-40 min. HPLC analysis after interesterification enzymatic reaction in mixture palm kernel olein (PKOo) and palm stearin (POs) showed that the composition of triglycerides (TAG) do not change if compared with the commercial lipase (Lypozyme TL1M).
Co-Authors . Ismiyati . Machfud Aam Aminah Abdul Aziz Darwis Adek Fitri Sakinah Agnes Febrynanti Woli Keban Agus Sudiman Tjokrowardojo Ali Ibrahim Hasyim Ali Mufti Anas Miftah Fauzi Anna Fatchiya Budiasih Wahyuntari Dasep Supriatna Dede Rais Deliza Zahra Rahmat Djoko Susanto Djumali Mangunwidjaja Djumali Mangunwidjaya Dwi Setyaningsih E. Gumbira Said Edy Mulyono Erliza Hambali Ervi Siti Nurfatwah Etin Maryatin Evi Savitri Iriani Faiz Abdul Malik Fembriarti Erry Prasmatiwi Firda A Syamani Gustan Pari Gustini Syahbirin Happy Yulia Hendri Darma Putra Hizmah Nurpardillah Illah Sailah Irpan Ilmi IS HELIANTI ISKANDAR ZULKARNAEN SIREGAR Kamal Firmansyah Khaswar Syamsu Laelatul Munawaroh Lena Apriyana Lestari, Dyah Aring Hepiana Liesbetini Hartoto Lisa Pratama Maylani Florensi Hutasoit Mira Rival Mochamad Noerdin N.Kurniadji Mohammad Syahid Syidiq Muhamad Asim Muhammad Rafiqi Setiadi NANDA AMALIA nFN Dadang nFN Risfaheri nFN Setyadjit Nurhalimah Nurhalimah Nurul Endah Setyani Rafika Tania Rani Satiti Reza Putri Krisna Wardhana Rianti Rianti Rina Novia Yanti Sara Gustia Wibowo SUBYAKTO SUBYAKTO Sudarma Widjaya Sukardi Sukardi Supriyanto Tatang Hidayat Tesar Dzikrulloh Titi C Sunarti Titi C. Sunarti Tun Tedja Irawadi Wasrin Syafii