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Journal : JURNAL RISET DIWA BAHARI

Pengaruh Kombinasi Probiotik Komersial Dan Bahan Herbal Terhadap Volume Flok dan Pertumbuhan Ikan Nila (Oreochromis niloticus) Nursyahran, Nursyahran; Ramadhani, Arda; Mulyawan, Ardi Eko
Jurnal Riset Diwa Bahari (JRDB) Volume 2, Nomor 2, 2024
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v2i2.37

Abstract

Biofloc technique is a cultivation method that utilizes microorganisms, especially heterotrophic bacteria, to convert toxic inorganic nitrogen waste, such as ammonia, into biomass that is beneficial for fish. In the biofloc system, heterotrophic bacteria play an important role in the formation of flocs, which are aggregates of microorganisms and organic particles that function as additional food sources for fish. These bacteria use nitrogen from ammonia in water to produce biomass, which can then be eaten by fish. This study aims to examine the effect of a combination of commercial probiotics and herbal ingredients on the growth of tilapia (Oreochromis niloticus). The method used in this study was an experiment with a Completely Randomized Design (CRD) consisting of four treatments and three replications. The treatments tested included treatment A (control), treatment B (EM4 + Ginger), treatment C (EM4 + Kencur), and treatment D (EM4 + Turmeric). This study was conducted for 35 days at the Moncongloe Maros Aquaculture Institute, with test fish in the form of tilapia seeds measuring 5-7 cm and aged 1 month. The results showed that the combination of commercial probiotics and herbal ingredients in treatment C (Probiotic EM4 + Kencur) produced the highest floc volume of 20.17 mL, while treatment D (EM4 + Turmeric) produced the lowest floc volume of 12.04 mL. Treatment C also recorded the highest Specific Growth Rate (SGR), which was 2.90%, while treatment D recorded the lowest SGR of 2.41%. Kencur is considered to have a positive effect because of its essential oil content which functions as a sedative effect, helps fish physiology, increases growth, maximizes protein digestibility, and increases feed efficiency. Anova analysis showed that SGR had a significant effect (P<0.05), while absolute length growth and survival rate did not show significant differences between treatments (P>0.05).
Efektivitas Penghambatan Ekstrak Daun Chaya (Cnidoscolus aconitifolius) terhadap Bakteri Vibrio harveyi Aldiwa, Nurul Fitri; Basir, Buana; Mulyawan, Ardi Eko
Jurnal Riset Diwa Bahari (JRDB) Volume 2, Nomor 2, 2024
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v2i2.38

Abstract

Vibrio harveyi bacteria are one of the main causes of mass mortality in shrimp in a short time. Chaya (Cnidoscolus aconitifolius) is a common plant that grows in many places in Central America, the Yucatan Peninsula of Mexico and has been widely cultivated in tropical and subtropical areas. The purpose of this study was to analyze the content of secondary metabolites in chaya leaves as antibacterials against Vibrio harveyi and to analyze the amount of inhibition of chaya leaves against Vibrio harveyi bacteria. The method used in this study was experimental with 4 treatments with 3 replications each, namely A (500 µg/mL chaya leaf extract) B (1000 µg/mL chaya leaf extract) C (Oxytetracycline antibiotic) and D (96% ethanol). The study was conducted from June to July 2024 at the Laboratory of Parasites and Fish Diseases, Faculty of Marine Sciences and Fisheries, Hasanuddin University. The results showed that the activity of chaya leaf extract (Cnidoscolus aconitifolius) did not show inhibition against V. harveyi bacteria at concentrations of 500 and 1000 µg/mL. Likewise, 96% ethanol did not show inhibition against V. harveyi bacteria. The inhibitory activity of Oxytetracyclin showed inhibitory activity with an average inhibition size of 9.26 mm. This is thought to be due to the low sensitivity of chaya leaf extract to V. harveyi bacteria. V. harveyi is known as a bacteria with a high level of malignancy when infecting, so it is thought to require a substance with stronger inhibitory activity.