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Rini B
Universitas Andalas
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Physicochemical and In Vivo Analysis of Rosemary (Rosmarinus officinalis L.) Extract Hair Tonic as a Natural Hair Growth Alternative Daimon Syukri; Rini Rini; Aisyah Putri; Reina Kurnia; Maria Makda Lena Br Simarmata; Diana Silvy; Hasbullah Hasbullah; Fakhrur Rozi; Erfina Efrina
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 10 No. 3 (2026)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v10i3.1249

Abstract

Hair loss is a common condition affecting both men and women and often diminishes quality of life. While synthetic agents like minoxidil are widely used to promote hair growth, their application is frequently associated with adverse side effects, highlighting the need for safer natural alternatives. This study aimed to evaluate the effectiveness of rosemary (Rosmarinus officinalis L.) extract in hair tonic preparations on hair growth and the product's physicochemical characteristics. Rosemary essential oil was extracted using steam distillation, then formulated into hair tonic at various concentrations (0%, 5%, 10%, and 15%), and compared with 2% minoxidil as a positive control. Physicochemical characterization included pH measurement, viscosity, and compound analysis using GC-MS. Hair growth activity was tested in vivo using male Wistar rats for 4 weeks, with hair length and weight as parameters. The results showed that all formulations had a pH of 4.18–4.75, corresponding to the physiological pH of the scalp. Viscosity increased with increasing rosemary extract concentration. GC-MS analysis revealed the presence of key compounds such as ?-myrcene, 1,8-cineole, and geraniol, which play a role in biological activity. In vivo testing showed that the 15% formulation produced the highest hair length (16.73 ± 1.97 mm), surpassing 2% minoxidil (10.02 ± 1.83 mm), while the 10% concentration produced the highest hair weight (87.083 ± 30.870 mg/cm²). Furthermore, no signs of irritation were observed in the rosemary group, while skin side effects were observed in the minoxidil group. It can be concluded that rosemary extract has the potential to be a natural active ingredient in an effective and safe hair tonic preparation to stimulate hair growth. A 10% concentration is recommended as the optimal formulation based on the balance between hair length and quality produced Contribution to Sustainable Development Goals (SDGs):SDG 3 – Good Health and Well-beingSDG 12 – Responsible Consumption and Production
Co-Authors Adhitya Jessica Adila Adisti Afissa Rahmadia Aisman Aisman Aisyah Putri Aisyah Putri Anak Agung Istri Sri Wiadnyani Anwar Kasim Arif Rizkika Putra Aurelia Amaliyah Tarumiyo Bastian Nova Cherie Dinah, Cherie Daimon Syukri Daimon Syukri Daimon Syukri Deden Dermawan Diah Ayu Natasya Diana Silvy Diana Sylvi Diana Sylvi Dina Afifah Zakia Doni Hariandi Ekawati, Fitri Erfina Efrina Fakhrur Rozi Fauzan Azima Felga Zulfia Rasdiana Firdaus Firdaus Haniyatul Halwa Hasbullah Hasbullah Heni Pujiastuti Husnul Khatimah Iin Hartati Wibisono Irfan Suliansyah Ismed Ismed Jaswandi . Laura Amelia Triani Luwina Makni Kusuma M. Fajar Syafrida Ma'ruf Ma'ruf Maria Makda Lena Br Simarmata Mimi Harni Mimi Harni Mimi Harni Mivtahul Dina Muhammad Arwani Muhammad Makky Najla Rosyadah Napassawan Liamnimitr Neswati Neswati Netty Sri Indeswari Nia Boru Ritonga Novelina Novizar Nazir Puja Nadia Sukma Purnama Dini Hari Rafifa Putria Dewita Ratni Prima Lita Reina Kurnia Reni Koja Rhenia Valeni Rilvi Muhammad Tio Rina Yenrina Ririn Fatma Nanda Risa Meuthia Fiana Rizkyanto, Ridwan Rudi Alfiansyah Sahadi Didi Ismanto Salsabila, Unik Hanifah Sayuti, Kesuma Tuty Anggraini Umar Ahmed Viony Derosya Wellyalina, Wellyalina Welya Refdi, Cesar Wenny Surya Murtius Yasmin Azzahra