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All Journal Jurnal Kedokteran Brawijaya e-Journal Pustaka Kesehatan Jurnal Ilmu Kesehatan Masyarakat STOMATOGNATIC- Jurnal Kedokteran Gigi Majalah Kedokteran Gigi Indonesia Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Health Notions Journal of Dentomaxillofacial Science Padjadjaran Journal of Dental Researchers and Students Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Jurnal Industri Pariwisata Health Dynamics

Rendra Chriestedy Prasetya

Fakultas Kedoktean Gigi Universitas Jember

Author-ID : 488478

Religion Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis Rendra Chriestedy Prasetya; Nunuk Purwanti; Tetiana Haniastuti
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Periodontitis adalah inflamasi kronis yang disebabkan oleh bakteri periodontopatogen. Pada periodontitis terjadi peningkatan infiltrasi neutrofil yang berfungsi untuk memfagositosis bakteri yang menginfiltrasi jaringan gingiva. Kulit manggis mempunyai bahan aktif yaitu xanton yang mempunyai efek antiinflamasi dengan jalan menghambat sintesis PGE2 sehingga akan menurunkan infiltrasi sel inflamasi. Penelitian ini bertujuan untuk mengetahui infiltrasi sel neutrofil pada periodontitis setelah pemberian ekstrak etanolik kulit manggis. Tikus wistar jantan sebanyak empat puluh delapan ekor diinduksi periodontitis dengan ligasi pada daerah subgingiva gigi anterior rahang bawah selama 7 hari. Setelah hari ke-7, ligasi dilepas selanjutnya tikus dibagi menjadi 4 kelompok perlakuan yaitu ekstrak kulit manggis 30 mg/kg BB dan 60 mg/kg BB, ibuprofen dan saline. Tikus dikorbankan pada hari ke-1, 3, 5 dan 7 setelah perlakuan. Jaringan pada bagian anterior rahang bawah ditanam dalam paraffin dan dilakukan pemotongan serial kemudian diwarnai dengan hematoxylin eosin. Jumlah neutrofil dihitung di bawah mikroskop dengan perbesaran 400x. Data jumlah neutrofil dianalisa dengan Two Way Anova. Hasil Two Way Anova menunjukkan perbedaan yang bermakna rerata sel neutrofil antar kelompok perlakuan (p<0,05) mengindikasikan pemberian ekstrak etanolik kulit manggis berpengaruh terhadap jumlah sel neutrofil. Dapat disimpulkan bahwa pemberian ekstrak etanolik kulit manggis mampu menurunkan infiltrasi sel neutrofil pada tikus yang diinduksi periodontitis.Neutrophil infiltration in rats with periodontitis after the granting of Ethanolic Extract Skin Mangosteen. Periodontitis is a chronic inflammatory disease caused by periodontopathic bacteria. When periodontitis occurs are followed by neutrophil infiltration. Mangosteen rind contains xanthone, an anti-inflammatory substance which inhibits the synthesis of PGE2 and therefore reduces inflammatory cells infiltration. This research aimed to study neutrophil cells infiltration in experimental-induced periodontitis rats after mangosteen rind ethanolic extract administration. Forty-eight male wistar rats were induced the periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for seven days. After the ligation had been taken out, the rats were divided into four groups and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd, 4th, seventh day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with hematoxylin-eosin. The neutrophil cells were observed and counted under the microscope (400x). The data were analyzed using Anova. Anova result showed a significant difference among group (p<0,05) indicating that mangosteen rind ethanolic extract affected the number of neutrophils. In conclusion, mangosteen rind ethanolic extract reduced the number of neutrophil infiltration in periodontitis rats.

Deteksi Lesi Aterosklerosis Koroner pada Model Tikus Periodontitis (Detection of Coronary Atherosclerotic Lesions in Periodontitis Rat Model) Roza Nafilah; Rendra Chriestedy Prasetya; I Dewa Ayu Susilawati
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background: Recent concept istated that periodontitis can induce the formation of coronary atherosclerotic lesions but there is less experimental study about that. Purpose: Analyze the effect of periodontitis on the formation of coronary atherosclerotic lesions.The parameters are coronary artery wall thickness, collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly. Methods: This experimental laboratoris (in vivo) study used male wistar rats with a total sample of 12 animals, were divided into 2 groups: control and periodontitis treatment that were prepared by mounting wire ligature and Porphyromonas gingivalis injection (0,5 McFarland/1,5x108 CFU/ml) in the gingival sulcus left mandibular first molar teeth 3 times a week for 4 weeks. On 5th week, mice were secrificed and heart that contain the coronary arteries was taken. Heart were fixed , histological preparations were made and colored by Picrosirius Red and Oil Red O. Results: The Arterial walls of periodontitis group are significantly (p <0.05) thicker than the control group. the presence of collagen intimal disintegration, atheroma, stenosis, endothelial disintegration, lipid deposition and fatty emboly are also significantly (p<0,05) more in periodontitis group than in the control group. Conclusion: Periodontitis increase the risk of coronary atherosclerotic. Keywords: Atherosclerosis Lesion, Periodontitis

GAMBARAN PERILAKU KESEHATAN GIGI ANAK SEKOLAH DASAR DI DESA BANGSALSARI KABUPATEN JEMBER Nadie Fatimatuzzahro; Rendra Chriestedy Prasetya; Winda Amilia
Jurnal Ilmu Kesehatan Masyarakat Vol 12 No 2 (2016)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Jember

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Pendahuluan: Karies gigi merupakan penyakit dengan prevalensi tertinggi selainpenyakit periodontal. Penyakit ini menjadi masalah nasional di beberapa Negaraberkembang terutama di Indonesia.Tujuan Penelitian: Etiologi utama karies pada anak adalah pola makan. Kelompok usiasekolah dasar mempunyai kebiasan jajan yang lebih tinggi dari kelompok usia lainkhususnya makanan manis, sehingga menyebabkan meningkatnya indeks karies gigi.Kegiatan ini didanai oleh Kemenristekdikti, yang dilakukan pada 2 SD yaitu SDN 03 danSDN 04 Bangsalsari. Metode Penelitian: Jumlah responden adalah siswa kelas 3 sebanyak 70 orang di SDN 03Bangsalsari dan 51 orang di SDN 04 Bangsalsari yang diperoleh dengan menggunakanmetode total sampling. Penyuluhan dan praktek gosok gigi bersama dilakukan kepadapara siswa untuk memberi pengetahuan serta memperbaiki perilaku mengenai kesehatanrongga mulut.Hasil Penelitian: Hasil pemeriksaan menunjukkan indeks DMF-T rata-rata untuk siswaSDN 03 Bangsalsari yaitu 6,1, sedangkan rerata indeks DMF-T untuk siswa SDN 04Bangsalsari yaitu 5. Indeks gigi yang karies (D) lebih dominan yaitu sekitar 67%dibanding gigi yang telah dicabut (M) sebanyak 2% dan gigi yang telah ditambal(F) hanya 1%. Masih tinggi nya gigi yang karies (D) dibandingkan gigi yang sudahditambal (F) menunjukkan masih rendahnya pengetahuan dan kesadaran siswa SDserta orang tua dalam menjaga kesehatan rongga mulutnya. Status karies gigi padasiswa SDN 03 dan 04 Bangsalsari berada pada kategori Tinggi berdasarkan kriteriaWHO. Kata kunci : Karies gigi, Perilaku, Siswa SD, Indeks DMF-T

Ekspresi COX-2 setelah pemberian ekstrak etanolik kulit manggis (Garcinia mangostana Linn) pada tikus wistar (COX-2 expression after mangosteen rind (Garcinia mangostana Linn) etanolic extract administration in wistar rats) Rendra Chriestedy Prasetya; Tetiana Haniastuti; Nunuk Purwanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 4 (2013): December 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Cyclooxygenase is an enzyme for prostaglandins (PGs) synthesis from arachidonic acid. Cyclooxygenase have been characterized and named as COX-1 and COX-2. COX-1 is responsible for constitutive PGs production under physiological condition and maintains normal function. On the other hand, while COX-2 expression is inducible by cytokines and endotoxin. Periodontitis is a chronic inflammatory disease caused by anaerobic bacteria especially gram negative bacteria. The periodontitis occurrence is followed by increased of COX-2 expression. Mangosteen rind (Garcinia mangostana Linn) contains gamma mangostin which inhibits the synthesis of PGE2 through inhibition of COX-2 expression. Purpose: This research was aimed to study COX-2 expression in experimental-induced periodontitis in wistar rats after mangosteen rind etanolic extract administration. Methods: Forty eight male wistar rats were induced periodontitis by putting silk ligature subgingivally around the cervical of the anterior lower teeth for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline respectively. The rats were sacrificed on the 1st, 3rd, 4th, 7th day after the treatment. The rats’ anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with immunohistochemistry. COX-2 expression were observed and counted under the microscope (400x). The data were analyzed using kruskall wallis test. Results: Kruskal wallis test showed a significant difference COX-2 expression among group indicating that mangosteen rind etanolic extract affected COX-2 expression. Conclusion: Mangosteen rind etanolic extract reduced COX-2 expression in periodontitis rats.Latar belakang: Siklooksigenase adalah enzim yang mensintesis prostaglandin (PG) dari asam arakhidonat. Siklooksigenase dibagi menjadi 2 yaitu COX-1 dan COX-2. COX-1 bertanggung jawab pada sintesis PG dalam kondisi fisiologis dan mempertahankan fungsi normal, sedangkan ekspresi COX-2 dapat terinduksi oleh sitokin dan endotoksin. Periodontitis adalah penyakit peradangan kronis yang disebabkan oleh bakteri anaerob terutama bakteri gram negatif. Terjadinya periodontitis diikuti oleh peningkatan ekspresi COX-2. Kulit buah manggis (Garcinia mangostana Linn) mengandung mangostin gamma yang menghambat sintesis PGE2 melalui penghambatan COX-2. Tujuan: Penelitian ini bertujuan untuk meneliti ekspresi COX-2 pada tikus wistar jantan yang diinduksi periodontitis setelah pemberian ekstrak etanolik kulit manggis. Metode: Empat puluh delapan ekor tikus wistar jantan diinduksi periodontitis dengan meletakkan ligatur sutra pada subgingiva sevikal gigi anterior rahang bawah selama 7 hari. Setelah ligatur dilepas, tikus dibagi dalam 4 kelompok yaitu ekstrak kulit manggis dosis 60 mg/kg BB, 30 mg/kg BB, ibuprofen dan saline dengan pemberian secara peroral. Tikus didekapitasi pada hari ke-1,3, 5 dan 7 setelah perlakuan. Rahang bawah gigi depan dilakukan pemrosesan menjadi blok paraffin, dipotong serial dan dilakukan pewarnaan imunohistokimia. Ekspresi COX-2 diamati di bawah mikroskop dengan perbesaran 400x. Data pengamatan dianalisa dengan uji kruskall wallis. Hasil: Uji kruskall wallis menunjukkan terdapat perbedaan bermakna ekspresi COX-2 diantara kelompok perlakuan yang mengindikasikan bahwa ekstrak kulit manggis mempengaruhi ekspresi COX-2. Simpulan: Ektrak etanolik kulit manggis menurunkan ekspresi COX-2 pada tikus dengan periodontitis.

Ekspresi dan Peran Siklooksigenase-2 dalam Berbagai Penyakit di Rongga Mulut Rendra Chriestedy Prasetya
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 12 No 1 (2015)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Cyclooxygenase-2 is part of the cyclooxygenase enzyme that plays a role in the inflammatory process has produce of prostaglandins (PG) such as PGE2. Prostaglandin E2 plays an important role in the pathogenesis of periodontal disease because it leads to increased vascular permeability, vasodilatory, edema. This review intends to give an overview the role of COX-2 in oral diseases likely periodontitis to be better understood and the future of our research that may facilitate the development of diagnosis and therapies. Cyclooxygenase is enzyme that plays of catalyze biosynthesis of prostaglandins and contained in two forms of COX-1 and COX-2. Cyclooxygenase-2 activity has increased in inflammation especially wound formation and periodontitis. In periodontitis, invasion of lipopolysacharide can Increase COX-2 activity in smooth blood muscle that can lead atherosclerosis. COX-2 inhibition can lead healing process that characterized fibroblast formation.it is concluded that the presence COX-2 in gingival tissue and gingival crevicular fluid can be used as biomarkers about the severity of periodontal disease, healing process and risk of atherosclerosis.

Daya Hambat Ekstrak Biji Kopi Robusta (Coffea Canephora) terhadap Bakteri Porphyromonas gingivalis (in vitro) Rr. Nektara Titan Dianastri; Pudji Astuti; Rendra Chriestedy Prasetya
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 18 No 2 (2021)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v18i2.28060

Periodontitis is mostly caused by plaque and Pophyromonas gingivalis bacteria as the main cause. The outer membrane layer of the Porphyromonas gingivalis wall produces pathogenic virulence factors, such as lipopolysaccharides which will activate inflammatory cells and cause phagocytosis of antigens thereby triggering free radicals. Robusta coffee beans naturally contain caffeine, phenolic compounds, trigonellin, and chlorogenic acids as antibacterial and anti-inflammatory. The purpose of this study was to determine the inhibition of Robusta (Coffea canephora) coffee bean extract 0.5%; 0.75%; 1%; 1.25%; 1.5% and 3% on the growth of Porphyromonas gingivalis in vitro and to find out the lowest concentration of Robusta (Coffea canephora) coffee bean extract which has inhibitory effect on the growth of Porphyromonas gingivalis. In this study were divided into 8 treatment groups namely positive control, negative control, 0.5% robusta coffee bean extract, 0.75%, 1%, 1.25%, 1.5% and 3%. Petridish dishes containing TSA media that have been sterilized, added P. gingivalis suspension with density according to Mc standard. Farland Then a sterile white test blank with a diameter of 6 mm that is still sterile is placed on top of the bacterial growth media in accordance with the placement of the treatment group and dropped with all 8 treatment materials. After 24 hours incubated in a desiccator, the inhibition of robusta coffee bean extracts against the growth of Porphyromonas gingivalis bacteria was observed and data collection was done by measuring the inhibition zone using calipers. The results obtained robusta coffee bean extract at concentrations of 3%, 1.5%, 1.25% and 1%, have an antibacterial power which is suspected because Robusta coffee beans naturally contain ingredients such as caffeine, polyphenols and chlorogenic acids which have antibacterial activity while the robusta coffee bean extract with a concentration of 0.5% and 0.75% does not have antibacterial power against Pophyromonas gingivalis. Robusta coffee bean extract with a concentration of 1% is the smallest concentration of Robusta (Coffea canephora) coffee bean extract which can inhibit the growth of Porphyromonas gingivalis.

Jumlah sel makrofag gingiva tikus wistar jantan yang diinduksi periodontitis setelah pemberian ekstrak etanolik kulit manggis Amount of machropahges cells in periodontitis-induced wistar rats after mangosteen rind etanolic extract administration Rendra Chriestedy Prasetya
Journal of Dentomaxillofacial Science Vol. 12 No. 3 (2013): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v12i3.366

Periodontitis is characterized with macrophages infiltration that is used to fagocyt bacteria which infiltrated atgingiva. Mangosteen rind contains xantone, an anti-inflammatory substance which inhibits the synthesis of PGE2 andtherefore reduce inflammatory cells infiltration. This research aimed to study machropahges cells infiltration inperiodontitis-induced wistar rats after administration of mangosteen rind etanolic extract. Forty eight male wistar ratswere induced periodontitis for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treatedorally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline. The rats were sacrified on the 1st, 3rd, 4th, and 7th day after the treatment. Their anterior lower jaws were processed histologically and stained withhematoxylin eosin. Two-way anova test showed a significant difference among groups (p<0.05). It was concluded thatmangosteen rind etanolic extract reduced the number of macropaghes cells gingiva in periodontitis male wistar rats.

Efektivitas ekstrak daun cocor bebek (kalanchoe pinnata) terhadap waktu perdarahan (bleeding time) pada ekor mencit strain balb-cEffectiveness of kalanchoe pinnata leaf extract on bleeding time in the tail of balb-c mice strain Zainul Cholid; Rendra Chriestedy Prasetya; Benedicta Regina Phoebe Sukamto
Padjadjaran Journal of Dental Researchers and Students Vol 6, No 2 (2022): Juni 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v6i2.39618

ABSTRAKPendahuluan : Pembedahan dalam kedokteran gigi dapat menyebabkan suatu komplikasi seperti perdarahan. Daun cocor bebek berpotensi sebagai agen hemostasis karena mengandung flavonoid, tanin, saponin yang berperan dalam menghentikan perdarahan. Tujuan penelitian untuk mengetahui efektivitas ekstrak daun cocor bebek terhadap waktu perdarahan pada ekor mencit strain balb-c. Metode: Jenis penelitian eksperimen laboratoris dengan rancangan penelitian the post test only control group design. 20 ekor mencit diadaptasikan selama satu minggu, dibagi menjadi 3 kelompok. Kelompok kontrol negatif (K-) disondase Na-CMC 0,5%, kelompok kontrol positif (K+) disondase asam traneksamat dosis 0,065 mg/gr BB mencit, kelompok perlakuan disondase ekstrak daun cocor bebek dan dibagi menjadi 3 sub kelompok dosis 0,1 mg/gr BB mencit (P1), dosis 0,2 mg/gr BB mencit (P2), dosis 0,3 mg/gr BB mencit (P3) dalam Na-CMC 0,5%. Mencit dipuasakan selama 7 jam sebelum pemberian sondase, setelah 1 jam pemberian, dilakukan pemotongan sepanjang 0,5 cm dari ujung ekor. Darah diteteskan pada kertas whatman dengan waktu 30 detik per kotak. Data dianalisis dengan uji Kruskall Wallis dan Mann Whitney. Hasil: Rata-rata waktu perdarahan K-, K+, P1, P2, P3 adalah 7,750 menit, 3,875 menit, 5,250 menit, 3,750 menit, 4,250 menit. Hasil uji Kruskal Wallis menunjukkan signifikansi 0,015 (p<0,05) sehingga terdapat perbedaan pada seluruh kelompok. Hasil uji Mann Whitney menunjukkan perbedaan signifikan antara kelompok K+, P1, P2, P3 dengan K- (p<0,05) sedangkan pada kelompok K+, P1, P2, P3 tidak berbeda signifikan (p>0,05). Simpulan: Ekstrak daun cocor bebek efektif dalam memperpendek waktu perdarahan pada ekor mencit strain balb-cKata Kunci : Kalanchoe; waktu perdarahan ; hemostasis ABSTRACTIntroduction: Surgery in dentistry can cause bleeding complications. Cocor bebek leaves have the potential as a hemostatic agent because they contain flavonoids, tannins, saponins that can stop bleeding. This study aims to determine effectiveness of cocor bebek leaf extract on bleeding time in balb-c mice strain tails. Methods: This type of research is a laboratory experiment with the post test only control group design. 20 mices were adapted for one week then divided into 3 groups. Negative control group was given 0.5% Na-CMC (K-), positive control group was given tranexamic acid at a dose of 0.065 mg/gr BW (K+), treatment group was given cocor bebek leaf extract and divided into 3 sub-groups at a dose of 0.1 mg/gr BW (P1), 0.2 mg/gr BW (P2), 0.3 mg/gr BW (P3) in 0.5% Na-CMC. Mice were fasted for 7 hours before giving sondase, After 1 hour of administration, 0.5cm length was cut from tail tip. Blood was dripped on whatman paper for 30 seconds/box. Data were analyzed by Kruskall Wallis and Mann Whitney test. Results: Average bleeding time for K-, K+, P1, P2, P3 was 7,750 minutes, 3,875 minutes, 5,250 minutes, 3,750 minutes, 4,250 minutes. Results of Kruskal Wallis test showed a significance of 0.015 (p<0.05) so that there were differences in all groups. Results of Mann Whitney test showed a significant difference between the K+, P1, P2, P3 and K- groups (p<0.05) while the K+, P1, P2, P3 groups were not significantly different (p>0.05). Conclusion: Cocor bebek leaf extract was effective in shortening the bleeding time in the tails of balb-c mice strainKeywords: Kalanchoe; bleeding time; homeostasis

Uji antibakteri ekstrak daun singkong (manihot esculenta crantz) terhadap fusobacterium nucleatum dan aggregatibacter actinomycetemcomitans Antibacterial activity test of cassava leaves extract (manihot esculenta crantz) against fusobacterium nucleatum and aggregatibacter actinomycetemcomitans Zahara Meilawaty; Amandia Dewi Permana Shita; Rendra Chriestedy Prasetya; Agustin Wulan Suci Dharmayanti; Rido Tri Andika Firdyansyach; Dhea Ayu Dewanti
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 3 (2022): Desember 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i3.37875

ABSTRAKPendahuluan: Prevalensi periodontitis di Indonesia masih terbilang tinggi. Data Riskesdas 2018 menunjukkan persentase kasus periodontitis di Indonesia sebesar 74,1%. Periodontitis merupakan penyakit inflamasi pada jaringan periodontal yang disebabkan oleh mikroorganisme spesifik seperti Fusobacterium nucleatum dan Aggregatibacter actinomycetemcomitans. Perawatan suportif menggunakan antibiotik, seperti metronidazole, diperlukan dalam perawatan periodontitis tetapi penggunaan antibiotik dapat memberikan efek samping sehingga perlu digantikan oleh tanaman herbal yang memiliki efek samping minimal, yaitu daun singkong (Manihot esculenta crantz). Penelitian ini bertujuan untuk menganalisis daya antibakteri ekstrak daun singkong terhadap Fusobacterium nucleatum dan Aggregatibacter actinomycetemcomitans. Metode: Jenis penelitian in vitro experimental laboratories dengan rancangan penelitian post test only control group design. Daun singkong yang sudah teridentifikasi di ekstrak dengan metode maserasi. Ekstrak kasar yang didapatkan kemudian dijadikan ke dalam beberapa dosis (6,25; 12,5; 25; 50; 100; dan 200 µg/mL). Kelompok dosis tersebut kemudian diuji menggunakan metode disk diffusion dan dibandingkan dengan kontrol positif yaitu metronidazole dan kontrol negatif yaitu propilen glikol.Data hasil penelitian diuji normalitasnya menggunakan uji Shapiro Wilk dan uji homogenitas menggunakan Levene test. Selanjutnya dilakukan uji Mann Whitney untuk melihat perbedaan antara dua kelompok sampel. Hasil: Terdapat zona jernih pada sekeliling kertas cakram dengan ekstrak daun singkong dosis 200 µg/mL yang menandakan adanya hambatan pertumbuhan dari F. nucleatum dan A. actinomycetemcomitans. Hasil statistik terlihat adanya perbedaan yang bermakna antara kelompok ekstrak daun singkong dosis 200 µg/mL dan kontrol positif (p=0,009) untuk F. Nucleatum, dan p=0,05 untuk A. actinomycetemcomitans. Simpulan: Ekstrak daun singkong dapat menghambat pertumbuhan dari F. nucleatum dan A. actinomycetemcomitans.Kata kunci: ekstrak daun singkong; fusobacterium nucleatum; aggregatibacter actinomycetemcomitans; antibakteriABSTRACTIntroduction: The prevalence of periodontitis in Indonesia is relatively high. The 2018 RISKESDAS data shows that the percentage of periodontitis cases in Indonesia is 74.1%. Periodontitis is an inflammatory disease of the periodontal tissue caused by specific microorganisms or groups of microorganisms such as Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans. Supportive care using antibiotics, such as metronidazole, is required to treat periodontitis. However, antibiotics can have side effects, so they need to be replaced by herbal plants with minimal side effects, namely cassava leaves (Manihot esculenta Crantz). This study aims to analyze the antibacterial effect of cassava leaf extract against Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans. Methods: This study was an in vitro laboratory experimental study with a post-test only control group design. The identified cassava leaves were extracted by the maceration method. The crude extract obtained was then made into several doses. The dose group was then tested using the disk diffusion method and compared with the positive control, metronidazole, and the negative control, propylene glycol.The research data were tested for normality using the Shapiro Wilk test and the homogeneity test using the Levene test. Then the Mann Whitney test was carried out to see the difference between the two sample groups Results: The results showed a clear zone around the disc paper with a 200 µg/ml dose of cassava leaf extract, which indicates the growth inhibition of F. nucleatum and A. actinomycetemcomitans. The statistical results showed a significant difference between the 200 µg/ml group and the positive control(p= 0,009 for F. Nucleatum; p=0,05 for A. actinomycetemcomitans). Conclusion: Cassava leaves extract can inhibit the growth of F. nucleatum and A. actinomycetemcomitans.Keywords: cassava leaf extract; fusobacterium nucleatum; aggregatibacter actinomycetemcomitans; antibacterial

Radiographic analysis of teeth calcification of breastfed rat cubs of mothers consuming excessive Arabica coffee Apriyono, Dwi Kartika; Shita, Amandia Dewi Permana; Fatimatuzzahro, Nadie; Prasetya, Rendra Chriestedy; Umaimah, Ulfa
Majalah Kedokteran Gigi Indonesia Vol 9, No 2 (2023): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.80243

Lack of nutrient intake, especially calcium, can inhibit tooth development because calcium is the major component of the inorganic material that forms enamel and dentin. Caffeine in coffee can decrease calcium levels in the body. Arabica coffee has a very high caffeine content compared to other types of coffee. Excessive caffeine consumption can cause the inhibition of PTH secretion. As a result, the nursing mother's body cannot absorb calcium optimally. This makes the baby lack calcium intake which is used for the dental calcification process. This study analyzes radiographic images of tooth calcification of rat cubs during lactation period from rat mothers which consumed excess doses of Arabica coffee. Forty-eight breastfeeding rat mothers (n = 48) were divided into a control group and a treatment group. The control group consisted of 24 rat mothers that were given aquadest. The treatment group consisted of 24 nursing rat mothers, which were given coffee orally at a dose of 2.7 grams/200 grams/day. After the rat mothers gave birth (H + 1 to H + 18), every 4 rat cubs were euthanized, then the mandibular bones were X-rayed. Dental calcification was viewed and analyzed based on radiographic images. The teeth of rat cubs whose mothers consumed excess doses of Arabica coffee experienced a delay in the calcification stage compared to the teeth of normal rat cubs. Consumption of Arabica coffee by nursing mothers can cause a delay in the stage of dental calcification in rat cubs.

Co-Authors Agustin Wulan Suci Dharmayanti Amandia Dewi Permana Shita Annisa Furqoni Baladraf, Thabed Tholib Benedicta Regina Phoebe Sukamto Bertung Suryadharma, Bertung Depi Praharani Dhea Ayu Dewanti Dwi Kartika Apriyono Farchi, Ghafran Nailul Favinas Octa Nuri Tsalats I Dewa Ayu Susilawati Ida Bagus Suryaningrat Ishimora, Marina Erlysa Laksmi Indreswari Muhammad Nurul Amin Nadie Fatimatuzzahro Nunuk Purwanti Nurul Iffah, Astrid Ganadya Peni Pujiastuti Pintan Qorina Destianingrum Pranata, Dadin Gilang Pudji Astuti Putri, Normalita Sari Aulia Harwidyanti Rido Tri Andika Firdyansyach Rokhani Rokhani Roza Nafilah Rr. Nektara Titan Dianastri Rusdianto, Andrew Setiawan Sampurna, Hadi Shinta Puri Sri Hernawati Tantin Ermawati Umaimah, Ulfa Winda Amilia Zahara Meilawaty Zainul Cholid

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