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Made Sriasih, Made
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INOVASI MINUMAN HERBAL MIDANG UNTUK MENINGKATKAN NILAI TAMBAH TANAMAN OBAT KELUARGA DAN KESEHATAN MASYARAKAT DESA GONDANG KECAMATAN GANGGA KABUPATEN LOMBOK UTARA Astuti, Nanik Nia Puji; Cahyani, Utari Nur; Rahmi, Ulya; Sriasih, Made
Jurnal Wicara Vol 2 No 3 (2024): Jurnal Wicara Desa
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/wicara.v2i3.4204

Abstract

Herbal drinks have become a popular alternative in maintaining people's health and well-being, especially in the modern era filled with the need for a healthy lifestyle. In Gondang village, Gangga sub-district, North Lombok district, the abundant potential of family medicinal plants (TOGA) has not been fully utilized. To address this challenge, an innovative herbal drink for Gondang village (MIDANG) aimed at increasing the added value of TOGA and improving public health was introduced to the Gondang village community. With a holistic approach, this activity covers the technical aspects of MIDANG production and the socio-economic implications of this product development. It is hoped that the MIDANG Herbal Drink will be a positive step towards sustainable prosperity for the people of Gondang Village and its surroundings.
Performa Rapid Diagnostik Tes Hepatitis B dengan ELISA sebagai Gold Standar Andayani, I Gusti Ayu Sri; Rizki, Mohammad; Sriasih, Made; Tauhida, Ika Nurfitria
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9035

Abstract

The ELISA (Enzyme Linked Immunoassay) method has high sensitivity and specificity, qualitatively and quantitatively detecting Hepatitis B Surface Antigen (HBsAg) levels, but the process is long, expensive and requires special expertise, compared to the Rapid Diagnostic Test (RDT) as a rapid test which is more practical and cheaper. The aim of this study was to compare the sensitivity and specificity of the Entebe Rapid Test Diagnostic (ERTD) produced by PT. Hepatika Mataram, with ELISA as the gold standard in detecting HBsAg. The requirement for interpreting sensitivity and specificity results according to World Health Organization (WHO) standards is a minimum of 95%. Testing with a negative panel of 00 samples and a positive panel of 1 samples from Papua in 012 at -80℃ storage. Testing in April 023 at the Molecular Diagnostics and Research Laboratory, Mataram University Hospital. Statistical analysis using MedCal software. ERD HBsAg produced by PT. Hepatika Mataram has a sensitivity of 95.45%, specificity of 99.50%, positive predictive value of 93.59%, negative predictive value of 99.65%, and accuracy of 99.21%.
Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif Andayani, I Gusti Ayu Sri; Iswaini, Dedy; Depamede, Sulaiman N; Kisworo, Djoko; Bulkaini, Bulkaini; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14304

Abstract

This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
Hanimal: Inovasi Website Pemanggilan Dokter Hewan untuk Edukasi Masyarakat dan Penanganan Kesehatan Hewan Rohim, Abdul; Sriasih, Made; Ilham, Moh. Rozi Fatwa; Adawiyah, Rabiatul; Ulya, Uswatul; Septiyawan, Pradino
Jurnal Pengabdian Inovasi Masyarakat Indonesia Vol. 3 No. 2 (2024): Edisi Agustus
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpimi.v3i2.5087

Abstract

Kesehatan hewan mencakup perlindungan sumber daya hewan, lingkungan, kesejahteraan hewan, dan ketahanan pangan asal hewan. Gangguan kesehatan hewan yang muncul saat ini dapat mempengaruhi kesehatan manusia, baik melalui konsumsi maupun interaksi langsung. Oleh karena itu, menjaga kesehatan hewan menjadi sangat penting, baik secara mandiri maupun dengan bantuan dokter hewan. Dokter hewan adalah garda terdepan dalam pelayanan kesehatan hewan, namun mereka menghadapi tantangan dalam penyampaian informasi yang efektif jika hanya menggunakan media WhatsApp. WhatsApp memungkinkan komunikasi cepat tetapi memiliki keterbatasan dalam penyampaian informasi secara terstruktur dan komprehensif. Banyak pemilik hewan mengalami kesulitan membawa hewan mereka ke dokter hewan karena masalah seperti sulitnya membuat janji, kurangnya pemahaman, dan jarak yang jauh. Survei menunjukkan bahwa 62% pemilik hewan tidak tahu apa yang harus dilakukan ketika hewan mereka sakit. Dr. Munawaroh dari PDHI menyatakan bahwa penyebaran dokter hewan di Indonesia masih kurang merata, lebih banyak di kota dibandingkan di desa, dengan jumlah dokter hewan yang tidak mencapai setengah dari yang diperlukan. Menanggapi masalah ini, Tim mengembangkan Hanimal, sebuah produk pelayanan dokter hewan berbasis website yang menawarkan jasa dokter hewan dengan harga terjangkau dan informasi lengkap serta berita terbaru melalui kerjasama dengan berbagai stakeholder, untuk meningkatkan akses dan efisiensi layanan kesehatan hewan.
Detection and Antibiotic Resistance of Salmonella spp. in Peranakan Etawa Goats Rosyidi, Anwar; Ali, Muhammad; Sriasih, Made; Depamede, Sulaiman N.; Wariata, Wayan; Yusuf, Muhammad Deni
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11787

Abstract

Diseases in goats reduce productivity and may cause reproductive disorders and zoonotic risks. Salmonellosis, caused by Salmonella spp., is an important livestock disease that can infect ruminants and poultry and contaminate animal products, affecting animal and human health. The purpose of this study was to determine the presence and the prevalence of Salmonella in Peranakan Etawa (PE) goats and to evaluate the antibiotic resistance profile of the isolates. Fecal samples were collected from 20 Peranakan Etawa goats and examined for the presence of Salmonella. Data on Salmonella cases and inhibition zone diameters (mm) obtained from antibiotic susceptibility testing were analyzed descriptively. The goats were managed under a semi-intensive system. They were fed odot grass supplemented with concentrate and silage additives, provided with well water as a drinking source, with feed troughs cleaned once daily and manure removed weekly. No anthelmintic treatment had been administered during the previous eight months. The results indicated that Salmonella infection was detected in 5% of the PE goats. The isolates showed the highest sensitivity to ciprofloxacin (25 mm inhibition zone), followed by chloramphenicol (8 mm) and tetracycline (7.33 mm), while resistance was observed against ampicillin.  
Co-Authors Abdul Rohim, Abdul Adji Santoso Dradjat Aini, Aini Aminurrahman Andayani, I Gusti Ayu Sri Anggraini, Syarifa Ayu Anwar Rosyidi Ardiana Ekawanti ASTUTI, NANIK NIA PUJI Azis, M Abdul Berliana, Cok Gea Bulkaini (Bulkaini) Cahyani, Utari Nur Depamede, Sulaiman N Djoko Kisworo Hasma Hasma Ilham, Moh. Rozi Fatwa Ima Arum Lestarini Imron Hadi Ine Karni Iswaini, Dedy Kalia, Ragil Alfarizi Khairil Anwar Lestari, Nur Ayu Mardi, Husnul Maulidia Umami Mizwar, Zulham Muhamad Ali MUHAMMAD ALI Pratama, Iman Surya Putra, Ryan Aryadin Rabiatul Adawiyah Rahmi, Ulya Ranesa, Sulhan Salma Rezki Amalyadi Rina Andriati, Rina Rizki, Mohammad Rosyidi, Muhammad Subhan Bahruddin Sahrani, Rani Sanhuri, Sanhuri Septian, I Gede Nano Septiyawan, Pradino Sukarne Sukarne Sulaiman Ngongu Depamede Suryadi, Muh. Aodil Fitriyan Fadjar Taqiuddin, Moh. Tauhida, Ika Nurfitria Ulya, Uswatul Unsunnidhal, Lalu Wariata, Wayan Widianti, Heny Yunita Sabrina, Yunita Yusuf, Muhammad Deni Zaid Al Gifari