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Hubungan Ekspresi LMP-1 dengan Overall Survival Pasien Diffuse Large B-Cell Lymphoma di RSUP Dr Kariadi Semarang Hamidiyah, Silpi; Sadhana, Udadi; Istiadi, Hermawan; Karlowee, Vega; Miranti, Ika Pawitra
Medica Arteriana (Med-Art) Vol 4, No 1 (2022): Juni 2022
Publisher : University of Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/medart.4.1.2022.18-25

Latar Belakang: Latent membrane protein 1 (LMP1) merupakan onkoprotein abnormal yang paling utama ditemukan pada infeksi virus Epstein-Barr (EBV). LMP1 sering terekspresi pada berbagai keganasan terkait EBV, salah satunya adalah diffuse large B cell lymphoma (DLBCL). Beberapa literatur telah menjelaskan keterlibatan EBV terhadap outcome klinis pasien namun nilai prognostik dari ekspresi LMP1 terhadap overall survival pasien DLBCL terkait EBV masih belum dapat dijelaskan.Metode: Penelitian dengan desain cross sectional ini melibatkan 50 pasien DLBCL yang didiagnosis antara Januari 2017 sampai Juni 2018. Pengamatan dilakukan selama 60 bulan. Data yang dikumpulkan meliputi usia, gejala B, stadium tumor, subtipe molekuler, dan survival 5 tahun. Analisis data menggunakan uji beda dan kurva Kaplan-Meier.Hasil: Dari 50 pasien, sebagian besar berusia diatas 40 tahun (88%), jenis kelamin laki-laki (60%), subtipe non GCB (62%), mengeluhkan semua gejala B (30%) dan stadium I (38%). Sebanyak 21 pasien meninggal (42%). Pasien dengan skor LMP1 yang tinggi menunjukkan overall survival yang lebih buruk secara bermakna dibandingkan dengan skor LMP1 yang rendah (P <0.001).Kesimpulan: DLBCL yang mengekspresikan LMP1 berhubungan dengan rendahnya overall survival 5 tahun dibandingkan dengan yang tidak mengekspresikan LMP1.

Healing of Radiation Dermatitis with Ozonated Aloe Vera Oil by Increasing PDGF and Epidermal Thickness in Sprague-Dawley Rats Utami, Widoasti Putri; , Yan Wisnu Prajoko; Prihharsanti, Christina H.N.; Sadhana, Udadi; Susilaningsih, Neni; Restiwijaya, Maryam; Nur, Muhammad
Folia Medica Indonesiana Vol. 58, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Highlights: • This study demonstrated the effect of ozonated Aloe vera oil in the healing of radiation dermatitis wound. • Groups that received no treatment was compared with groups that received treatment using 2.5% hydrocortisone ointment and ozonated Aloe vera. • Ozonated Aloe vera oil improves the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness. Abstract : The long-term use of corticosteroids as a standard treatment for skin disorders, such as radiation dermatitis, can cause many side effects. Alternatively, ozonated Aloe vera oil may replace corticosteroids due to its fewer side effects and benefits in wound healing process. Re-epithelialization and the formation of growth factors, such as platelet-derived growth factor (PDGF), play an important role in the healing of dermatitis wound. This study intended to demonstrate the effect of ozonated Aloe vera oil to improve the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness. This study used a post-test only control group design. A sample of 36 Sprague-Dawley rats was divided into 6 groups (C1=without treatment, C2=2.5% hydrocortisone ointment, P1=pure Aloe vera (AV), P2=300 mg/mL ozonated Aloe vera (OAV), P3=600 mg/mL OAV, P4=1200 mg/mL OAV. The expression of PDGF was assessed using Allred scoring with immunohistochemical staining, whereas the epidermal thickness was assessed using hematoxylin and eosin (H&E) staining at 400x microscopic magnification. The PDGF expression and epidermal thickness between the control and the treatment groups showed significant differences using a Kruskal-Wallis test (P=0.001) and one-way ANOVA test (P<0.001). The groups that was given ozonated Aloe vera oil had higher average of PDGF expression and thicker epidermis than the other groups. The Spearman's correlation test showed a strong positive relationship (p<0.001 and r=0.709) between the two variables. In conclusion, ozonated Aloe vera oil improves healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness.

ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS Ningtyas, Endah Ariyati Eko; Santoso, Oedijani; Sadhana, Udadi; Sunarintyas, Siti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

The Effects of Kretek Cigarettes and Ascorbic Acid-based Vape on IL-6, TNF-α Levels, and Pulmonary Histopathology: An Experimental Study on White Rats (Rattus novergicus) on Inflammatory Response and Pulmonary Histopathology Maftikhati, Dora; Prasetyo, Awal; Sadhana, Udadi; Istiadi, Hermawan
Diponegoro International Medical Journal Vol 6, No 2 (2025): December 2025
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dimj.v6i2.29825

Background: Smoking habits remain difficult to break, with 991 million smokers worldwide in 2020. Many have switched to vaping, which is regarded as safer than clove cigarettes. Exposure to cigarette smoke can increase pro-inflammatory cytokines and damage lung tissue.Objective: This study compared the effects of kretek cigarette smoke and ascorbic acid-based vape smoke on inflammatory response and lung tissue in male white rats (Rattus norvegicus).Methods: Twenty-one 8-week-old rats were randomized into three groups: control, exposed to kretek cigarette smoke, and exposed to ascorbic acid-based vape. The kretek cigarette group was exposed to smoke from 3 cigarettes/day, while the vape group received 0.5 ml/day of vape smoke, for 12 weeks. Interleukin-6 (IL-6) and Tumor Necrosis Factor-alpha (TNF-α) levels were analyzed using ELISA. The perimeter length of the alveolus, the degree of alveolar wall damage, and the extent of inflammatory cell distribution were also examined. Statistical analyses were accomplished using the ANOVA One-Way test, chi square test, and Kruskal-Wallis test.Results: Cigarette exposure significantly increased IL-6 levels (control: 8.43 ± 0.88 pg/ml; kretek cigarette: 11.45 ± 1.17 pg/ml; ascorbic acid vape: 11.83 ± 1.56 pg/ml; p = 0.000), the degree of alveolar damage (mean rank control: 6.21; kretek cigarette: 14.17; ascorbic acid vape: 11.64; p = 0.001), and the extent of inflammatory cell distribution (mean rank control: 4.00; kretek cigarette: 15.25; ascorbic acid vape: 12.93; p = 0.012). TNF-α levels increased in the kretek cigarette group, while the alveolar perimeter length increased in the ascorbic acid vape group; however, neither parameter was statistically significant (p > 0.05).Conclusion: Both kretek cigarette smoke and ascorbic acid-based vape smoke induced pulmonary inflammation and structural changes, with significant effects observed in IL-6 levels and histopathological damage, but not in TNF-α levels or alveolar perimeter length. There is a need for better regulation and increased public awareness about the dangers of smoking.

ROLE OF COMBINATION CASEIN AND LACTOFERRIN BOVINE’S COLLOSTRUM AS A PULP CAPPING ON MACROPHAGE EXPRESSION IN MALE WISTAR RATS Ningtyas, Endah Ariyati Eko; Santoso, Oedijani; Sadhana, Udadi; Sunarintyas, Siti
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.156-164

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.

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