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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Prosiding Seminar Biologi Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Jurnal Sains Alami (Known Nature) KOPEMAS
Tintrim Rahayu, Tintrim
Universitas Islam Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Aplikasi Fermentasi Limbah Padat Ampas Tahu Dan Ampas Tempe sebagai Pupuk Organik terhadap Pertumbuhan Tanaman Kenikir (Cosmos audatus): Application of Fermented Tofu Solid Waste and Tempe Dregs as Organic Fertilizer on Growth Kenikir Plant (Cosmos audatus) Arifyani, Laily Febrian; Laili, Saimul; Rahayu, Tintrim
Jurnal SAINS ALAMI (Known Nature) Vol. 7 No. 1 (2024)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v7i1.18478

Abstract

Tofu and tempeh are foods that are liked by the community. From the processing of tofu and tempeh will produce waste. Many people are thrown away, even though it can be used as organic fertilizer for plant growth. tofu Tofu dregs contain nitrogen (N) on average 16% of the protein it contains. This study aims to analyze the effect of fermenting tofu and tempeh liquid waste on the growth of kenikir (Cosmos caudatus) plants. The design used in this study was a randomized block design consisting of 2 factors. The first factor is giving fermented tofu waste which consists of 4 levels, namely 50, 100,, 200 and 300 g per plant, the second factor is giving fermented tempeh solid waste which consists of 4 levels, namely 50, 100,, 200 and 300 g, 0 grams as a control so there are 9 treatments in total. And each treatment has 3 replications. Parameters observed in this study were plant height, number of leaves, wet and dry weight of plants, leaf area and root length. The results showed that the fermentation of tofu and tempeh waste had a significant effect on the growth of kenikir plants with the parameters of plant height, number of leaves, leaf area, leaf area, wet weight and dry weight of the plant. The treatment of solid waste fermentation of 300 grams of tofu from planting is the best treatment in accelerating the growth of plant length, wet weight, dry weight, wet weight, number of leaves, leaf areainplants. Keywords: tofu dregs, tempeh dregs, kenikir plant (Cosmos caudatus) ABSTRAK Tahu dan tempeImerupakan makanan yangIdisukai Masyarakat. Dari prosesIpengolahan tahu danItempe akan menghasilkan limbah. Banyak kalanganImasyarakat yang dibuang begitu saja padahal bisa dijadikan sebagai pupuk organik untukIpertumbuhan tanaman. Ampas tahuImengandung unsure nitrogenI (N) rata-rata 16% dari protein yangIdikandungnya. Penelitian ini bertujuanIuntuk menganalisis pengaruhIpemberian fermentasi limbah cair tahuIdan tempe untuk pertumbuhanItanaman tanaman kenikir (Cosmos caudatus). RancanganIyang digunakan dalam penelitianIini adalah rancangan acak kelompokIyang terdiri dariI2 faktor. FaktorIpertama pemberian fermentasiIlimbah tahu yang terdiri atas 4 taraf yaituI50, 100, 200 dan 300 g per tanaman, faktor keduaIpemberian fermentasi limbah padat tempe yang terdiriIatas 4 taraf yaitu 50, 100, 200 dan 300 g. 0 gram sebagai controlIsehingga total terdapat 9 perlakuan. Dan setiap perlakuanIterdapat 3 kali ulanagn. Parameter yang diamatiIpada penelitian ini adalah tinggi tanaman, jumlah daun, berat basah danIberat kering pada tanaman, luasIdaun dan Panjang akar. Hasil penelitianImenunjukan pemberian fermentasiIlimbah tahu dan tempe berpengaruhInyata terhadap pertumbuhan tanamanIkenikir dengan parameter tinggi tanaman, Ijumlah daun luas daun, luas daun, beratIbasah dan berat kering tanaman. Perlakuan fermentasi limbah padat tahu 300 gIpertanaman merupakan perlakuanIterbaik dalam mempercepat pertumbuhan panjang tanaman, berat basah, berat kering, beratbasah, jumlah daun,luas daun pada tanaman. Kata kunci: ampas tahu, ampas tempe, tanaman kenikir (Cosmos caudatus).
Pengaruh Ekstrak Kulit Umbi Bawang Merah (Allium cepa) dan Bawang Putih (Allium sativum) untuk Pertumbuhan Anggrek Dendrobium: The Effect of Shallot (Allium cepa) and Garlic (Allium sativum) Skin Extracts on Dendrobium Orchid Growth Marista, Bilqis; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 6 No. 2 (2024)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v6i2.18809

Abstract

Genetic and physiological factors influence dendrobium orchids growth and flowering. In maintaining Dendrobium orchids, the cultivation process after acclimatization greatly affects growth and development, so its quality and sustainability can be threatened. Onion bulb skin and garlic bulb skin were able to increase germination and growth variables. This research aims to determine the effect of the most effective concentration on the growth of Dendrobium orchids. This research method used an experimental method with a completely randomized design (CRD) with 24 plants, research parameters included the number of new shoots, number of new shoots, number of new roots, new root length, leaf length, total number of leaves, plant height, and weight. Plants were analyzed descriptively, and multivariate correlation tests were using IBM software. The results showed that the M40: P20 treatment was the best treatment with parameters that responded, namely the number of new shoots, length of new roots, length of leaves, total number of leaves and plant height. This shows that the administration of shallot bulb skin extract and garlic bulb skin extract positively affected several parameters. Keywords : acclimatization, Dendrobium, effectiveness, parameters, plants.   ABSTRAK Anggrek Dendrobium dalam pertumbuhan dan pembungaan dipengaruhi faktor genetik dan faktor fisiologis. Dalam pemeliharaan anggrek Dendrobium proses budidaya setelah aklimatisasi sangat mempengaruhi pertumbuhan dan perkembangan, sehingga kualitas dan kelestraiannya dapat terancam. Kulit umbi bawang merah dan kulit umbi bawang putih mampu meningkatkan perkecambahan dan variabel pertumbuhan. Tujuan penelitian ini yaitu untuk mengetahui pengaruh dari konsentrasi yang paling efektif terhadap pertumbuhan anggrek Dendrobium. Metode penelitian menggunakan eksperimental Rancangan Acak Lengkap (RAL) dengan 24 tanaman, parameter penelitian meliputi jumlah tunas baru, jumlah daun tunas baru, jumlah akar baru, panjang akar baru, panjang daun, total jumlah daun, tinggi tanaman, dan berat kering tanaman yang dianalisis secara deskriptif dan uji korelasi multivariat test menggunakan software IBM. Hasil penelitian menunjukkan perlakuan M40: P20 adalah perlakuan terbaik dengan parameter yang merespon yaitu jumlah daun tunas baru, panjang akar baru, panjang daun, jumlah total daun dan tinggi tanaman. Hal ini menunjukkan bahwa pemberian ekstrak kulit umbi bawang merah dan kulit umbi bawang putih memberikan pengaruh positif pada beberapa perameter. Kata kunci: aklimatisasi, Dendrobium, efektif, parameter, tanaman.
The Effect of the Type of Planting Media and The Addition of O2 Nanobubbles on the Growth of Dendrobium burana Green × Ong Ang Ai Boon Orchid Plantlets in Vitro Purnamasari, Viska; Rahayu, Tintrim; Jayanti, Gatra Ervi; Agisimanto, Dita
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.41376

Abstract

Dendrobium orchids come in a variety of colors, shapes, and beauty that are in great demand. The process of growing orchids requires special handling of an appropriate growing environment, such as proper planting media and nutrients that can stimulate orchid growth. This type of thin liquid film media is the use of liquid media with an immersion system while vermiculite and perlite media have almost the same properties. However, vermiculite has a higher water absorption capacity than perlite. Nanobubbles are bubbles with 1-100 nm that can live more stably in water. This study aims to influence the type of media and the addition of Nanobubbles O2 on the growth of Dendrobium burana Green × Ong Ang Ai Boon orchid plantlets. This research was carried out from November to December 2022 at the PT. Java Indo Arjuna Singosari, Malang. This study used a completely randomized design (CRD) with 10 treatments, namely solid MS, gaviota solid, thin liquid film, thin liquid film + NBs O2, vermiculite, vermiculite + NBs O2, perlite, perlite + NBs O2, vermiculite + Perlite, vermiculite + perlite + NBs O2 with 4 replications. The data were analyzed descriptively and the Manova test. The results showed that the vermiculite + NBs O2 treatment had the potential to increase the length of plantlets by 13.80 mm, the wet weight of plantlets by 0.30 g, the percentage of live plantlets by 100% whereas in the vermiculite + perlite treatment has the potential for the first root growth to appear, namely 5 HST. The vermiculite treatment had the potential to increase the highest number of leaves by 5 leaves, whereas in the leaf color treatment the solid MS treatment had a more intense green color.
Potential of Ecoenzymes and N2 Nanobubbles on the Growth of Phalaenopsis sp. Orchid at the Acclimatization Stage Syafitri, Fitria Indah; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.40575

Abstract

Growth is a quantitative change during one plant’s life cycle and is irreversible.withaddition An in crease the volume or dry weight of plants or other organs is due to the adding, of new structural elements. Phalaenopsis (moth orchid) is a genus of orchids appeal. The diversity of colors, shapes, and textures, as well as its aroma, make Phalaenopsis orchids one of the charming flowers of Indonesia. Ecoenzyme is a solution of complex organic substances produced from the fermentation of organic residues, sugar, and water. Nanobubbles (NBs) are an example of nanotechnology. NBs have a diameter of 1-100 nm and are nanoscopic gas bubbles in a solution or water that can change the characteristics of water and are stable. This study aims to determine the potential of giving Ecoenzyme and NBs N2 on the growth of Phalaenopsis orchids during the acclimatization stage. This research was conducted from October to December 2022 at the Orchidology and Nursery Laboratory, Islamic University of Malang. This study used an experimental method with a completely randomized design (CRD) with 10 treatments, namely 0 mL/L (control), 1 mL/L Ecoenzyme, 2 mL/L Ecoenzyme, 3 mL/L Ecoenzyme, 4 mL/L Ecoenzyme, 4 combination NBs N2 and Ecoenzymes, and 5 mL NBs N2 with 3 replications, making a total of 30 treatment units. The research parameters included the number of leaves, leaf length, number of roots, root length, plant height, fresh weight, and plant dry weight. The results of this study showed that the treatment of 3 mL/L Ecoenzyme showed potential in increasing the number of leaves by 4 pieces: at the same time the optimal concentration of the combination of Ecoenzyme and NBs was 2 mL/L plus 5 mL NBs N2 in increasing the number of roots by 10 pieces, and the optimal concentration of NBs was 5 mL in increasing the root length by 7.07 cm, plant height by 17.10 cm leaf. length, plant weight by 4.16 g and dry weight by 0.85 g in Phalaenopsis orchids.
Analysis of Nanobubbles (NBs) Technology and Foliar Fertilization on the Growth of Phalaenopsis sp. Orchid Puspa, Andria; Rahayu, Tintrim; Jayanti, Gatra Ervi
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.39809

Abstract

Plants need nutrients which are then provided through fertilizers, but this can cause long-term problems due to limited absorption by plants so recently implemented NBs technology that can help reduce environmental pollution. This study aimed to determine the effect of NBsO2, NBsH2, NBsCO2, NBsN2 and, NBsO3 on the growth of Phalaenopsis sp. orchid plants. The research method was a complete randomized design (RAL) with 7 treatments (control without application of fertilizer and NBs, foliar fertilizer, NBsO2, NBsH2, NBsCO2, NBsN2, NBsO3) and 4 repeats. NBs were made using a nanogenerator with a flow of 5 liters/ minute and induced for 15 minutes. The data analysis used is the Multivariate Analysis of Variance (MANNOVA) test. The control and NBs treatments were given by dropping 5 ml on each orchid plant. The results showed that leaf length, number of leaves, plant weight and number of plant roots NBsN2 treatment had the best effect, while on root length, NBs O2 treatment had a better effect than other treatments. The application of foliar fertilizer is less effective to meet plant growth needs. At the same time, NBs are more effective because they are very small making it easier for plants to absorb.
EKSPLORASI PENGETAHUAN PEGAWAI SMK KEHUTANAN NEGERI SAMARINDA DALAM IN HOUSE TRAINING HERBARIUM KERING INDAH Ari Hayati; Tintrim Rahayu
Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) #5 2024 Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) 2020
Publisher : Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) #5 2024

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bagi Lembaga pertanian dan perkebunan penggunaan lembar identitas species yang memuat klasifikasi lengkap dengan deskripsi species tumbuhan sangatlah penting. Dalam hal ini spesimen berupa herbarium menjadi pelengkap lembar  identitas tersebut. SMK Kehutanan dalam  menunjang pendidikan siswanya memerlukan ketrampilan pembuatan herbarium. Tujuan kegiatan pengabdian ini adalah memberikan pelatihan pembuatan herbarium kering indah dengan tetap mempertahankan warna alami tumbuhan kepada pegawai SMK Kehutanan Negeri Samarinda. Metode pelaksanaan meliputi teknik dasar pengeringan bahan tumbuhan menggunakan bahan pengering berupa kertas buram,  kertas minyak, dan busa. Bahan tumbuhan diatur secara berlapis, kemudian disimpan di dalam plastik kedap air. Dilakukan proses pengeringan berulang-ulang sampai bahan tumbuhan menjadi  kering. Berikutnya teknik penataan herbarium di dalam pigura dengan tetap mempertahankan kekeringan bahan menggunakan cara alami dan penggunaan silika gel. Untuk mengetahui tingkat pengetahuan peserta pelatihan dilakukan analisis kuesioner tentang herbarium. Hasil kegiatan menunjukkan setiap peserta berhasil mempraktekkan teknik dasar pengeringan dan mampu menata herbarium secara indah dalam pigura. Hasil analisis kuesioner menunjukkan  sebelum dilakukan kegiatan pengetahuan tentang herbarium lebih rendah dibandingkan dengan setelah dilakukan pelatihan. Selain itu bahwa kebanyakan peserta sangat ingin mengetahui pembuatan herbarium yang tidak berubah warna dari warna aslinya. Disamping sebagai penunjang kelengkapan lembar identitas species tumbuhan, juga peluang  untuk hiasan atau asesori yang indah.
Aplikasi Fermentasi Limbah Industri Tahu Dan Tempe Sebagai Pupuk Organik Cair Terhadap Pertumbuhan Tanaman Kemangi (Ocimum basilicum): Application of Tofu and Tempeh Industrial Waste Fermentation as Liquid Organic Fertilizer for Basil Plant Growth (Ocimum basilicum) Hasanah, Rochmatul; Laili, Saimul; Rahayu, Tintrim
Jurnal SAINS ALAMI (Known Nature) Vol. 7 No. 2 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v7i2.18428

Abstract

Basil plants are vegetables and foodstuffs that are liked by the community, especially the people of Indonesia. Awareness of the importance of healthy living makes vegetables from agricultural products to be widely cultivated. This study aims to analyze the effect of fermenting tofu and tempeh liquid waste on the growth of basil plants. The design used in this study was a randomized block design consisting of two factors. The first factor is the fermentation of tofu liquid waste, which consists of four levels: 25 ml, 50 ml, 75 ml and 100 ml planting. The second factor is the fermentation of tempe liquid waste, which consists of four levels: 25 ml, 50 ml, 75 ml and 100 ml per plant and 0 ml as a control in total, there are nine treatments, and each treatment has three replications. Parameters observed in this study were plant height, number of leaves, wet and dry weight of plants, leaf area and root length. The results showed that the fermentation of tofu and tempeh liquid waste had a significant effect on the growth of the basil plant. Fermentation treatment of 100 ml tofu liquid waste from planting is the best treatment for accelerating the growth of plant length, root length, wet weight, dry weight, wet weight, number of leaves, and leaf area in plants. Keywords: fermentation, liquid waste, tofu, tempeh, basil Tanaman1kemangi merupakan sayuran1sekaligus bahan makanan1yang disenangi masyarakat1khususnya masyarakat Indonesia. Kesadaran akan1pentingnya hidup sehat menjadikan1sayur-sayuran produk1pertanian menjadi banyak1dibudidayakan. Penelitian ini1bertujuan untuk menganalisis pengaruh1pemberian fermentasi limbah cair tahu1dan tempe untuk pertumbuhan tanaman1kemangi. Rancangan yang1digunakan dalam penelitian ini adalah1rancangan acak kelompok yang1terdiri dari 2 faktor. Faktor pertama1adalah fermentasi limbah cair tahu yang1terdiri dari 4 taraf yaitu 25 ml, 50 ml 75 ml dan 100 ml1pertanaman. dan faktor kedua yaitu fermentasi limbah1cair tempe yang terdiri dari 4 taraf yaitu 251ml, 50 ml, 75 ml dan 100 ml per tanaman1dan 0 ml sebagai kontrol sehingga total1terdapat 9 perlakuan, setiap perlakuan1terdapat 3 kali ulangan. Parameter1yang diamati pada penelitian ini1adalah tinggi tanaman, jumlah1daun, berat basah dan kering pada1tanaman, luas daun dan panjang akar. Hasil penelitian1menunjukan pemberian fermentasi1limbah cair tahu dan tempe berpengaruh nyata terhadap pertumbuhan1tanaman kemangi. Perlakuan fermentasi1limbah cair tahu 100 ml pertanaman merupakan perlakuan1terbaik dalam mempercepat pertumbuhan panjang1tanaman, panjang akar, berat basah, berat1kering, berat basah, jumlah1daun,luas daun pada tanaman. Kata kunci: fermentasi, limbah cair, tahu tempe, kemangi
The New Technology for In Vitro Culture with Induction of Nanobubbles (NBsN2 and NBsO2) in Cattleya sp. Mustakim, Moch; Mawardi, Azis; Dewi, Lina Purnama; Putri, Nela Vede Rhofa; Rahayu, Tintrim; Jayanti, Gatra Ervi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.459-465

Abstract

The growth and development of orchids take a relatively long time, while the demand for high-quality orchids continues to increase significantly. Tissue culture is a technique of isolating plant parts in organs, tissues, and cells and then culturing these plants on artificial media in a sterile environment. Tissue culture techniques can reproduce plants in a relatively short time, with the same properties and quality, so efforts to optimize tissue culture with technology are the right solution, one of which is using nanogenerator technology that produces Nanobubbles (NBs). NBs are one of the nanotechnologies that are ?100 nm in size with induced gas needed by plants. This study aims to determine the effectiveness of growth and development from the induction of NBsN2 and NBsO2 in Cattleya sp. planlet in vitro culture. This research method uses in vitro culture with a Randomized Group Design. The results of the MsO treatment showed higher weight than other treatments, with a total plant weight value of 0.3550 g and a total plant height value of 1.3983 cm. Murashige-Skoog + NBsNitrogen (MsN) treatment showed higher root length than other treatments with a total value of 1.2367 cm. In the treatment of Ms, MsO, OJ and NJ, a live percentage of 83-100% was observed. The statistical test results indicate that NBs positively affect Cattleya sp. plantlets. This effect includes increasing plant weight, height, and root length and promoting new shoots growth. Additionally, nitrogen supplements play a role in colour changes, indicating the plant's health and photosynthetic efficiency. In the treatment with Induction O2, the initial colour changes from Strong Yellow Green (141 D) to Strong Yellowish Green (141 C), while in the treatment with Induction NBsN2, the initial colour changes from Strong Yellow Green (141 D) to Deep Yellow Green (141 B).
Pengaruh Spektrum Warna dan Penambahan Nanobubbles (NBs) Nitrogen (N2) terhadap Pertumbuhan Anggrek Dendrobium sp. secara In Vitro: The Influence of Color Spectrum and Addition of Nitrogen (N2) Nanobubbles (NBs) on the In Vitro Growth of Dendrobium sp. Orchids Salsabilla, Dyah Ajeng; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18811

Abstract

In in vitro culture, light and nitrogen absorption play crucial roles in the growth of Dendrobium sp orchids. Light affects plant absorption; thus, nitrogen must be added in the right amount for optimal growth. Nanobubbles (NBs) technology has been developed to convert nitrogen into smaller particles, facilitating plant absorption. This study aims to investigate the effects of color spectrum and N2 NBs on the growth of Dendrobium sp orchids. The experimental research method involved different treatments of media (MS; MS+ZPT; MS+ NBs N2; MS+ZPT+NBs N2) and color spectrum (white, red, blue, red-blue). Data on live percentage, plantlet legth and plantlet weight were analyzed descriptively and statistically (normality, homogeneity, multivariate, and Games-Howell tests). The results showed that the highest percentage of live plantlets, 97.91%, was found in the treatments with red and red-blue color spectrum. The red-blue color spectrum significantly increased the plantlet weight by 0.363 g in the MS treatment, and the application of red color spectrum potentially increased the plantlet length by 1.707 mm in the MS+ZPT treatment. The addition of NBs N2 in the subculture media showed potential for 100% live plantlet percentage in the MS+NBs N2 treatment. Keywords: Dendrobium, nanobubbles (NBs), color spectrum, in vitro, Nitrogen ABSTRAK Dalam kultur in vitro, penyerapan cahaya dan nitrogen berperan penting terhadap pertumbuhan anggrek Dendrobium sp. Cahaya mempengaruhi penyerapan tanaman, sehingga nitrogen harus ditambahkan dalam jumlah yang tepat untuk pertumbuhan optimal. Teknologi Nanobubbles (NBs) telah dikembangkan untuk mengubah nitrogen menjadi partikel yang lebih kecil agar memudahkan penyerapan oleh tanaman. Penelitian ini bertujuan untuk mengetahui pemberian spektrum warna dan NBs  terhadap pertumbuhan anggrek Dendrobium sp. Metode penelitian eksperimental dengan perlakuan media (MS; MS ₊ ZPT; MS ₊ NBs ; MS ₊ ZPT ₊ NBs ) dan spektrum warna (putih, merah, biru, merah-biru). Data persentase hidup, panjang planlet dan berat planlet dianalisis secara deskriptif dan uji statistik (normalitas, homogenitas, multivariat, dan Games Howell). Hasil penelitian menunjukkan bahwa pada persentase hidup planlet tertinggi yaitu 97,91% terdapat pada perlakuan spektrum warna merah dan merah-biru. Spektrum warna merah-biru berpengaruh dalam meningkatkan berat planlet sebesar 0,363 g pada perlakuan MS dan pemberian spektrum warna merah berpotensi meningkatkan panjang planlet sebesar 1,707 mm pada perlakuan MS + ZPT. Dalam pemberian NBs  dalam media subkultur memberikan potensi persentase hidup planlet sebesar 100% pada perlakuan MS ₊ NBs .  Kata kunci: Dendrobium, Nanobubbles (NBs), Spektrum warna, Nitrogen
Profil Senyawa Metabolit Sekunder dan Deteksi Antosianin pada Buah Stroberi (fragaria x ananassa) Menggunakan Kromatografi Lapis Tipis dan Spektrofotometri Inframerah: Profile of Secondary Metabolite Compounds and Anthocyanin Detection in Strawberry Fruit (Fragaria x ananassa) Using Thin-Layer Chromatography and Infrared Spectrophotometry Haikal, Muhammad Wildan; Rahayu, Tintrim; Jayanti, Gatra Ervi
Jurnal SAINS ALAMI (Known Nature) Vol. 8 No. 1 (2025)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v8i1.18812

Abstract

Strawberry plants are a type of plant that grows extensively in Indonesia. Some studies show that Strawberries (Fragaria x ananassa) are a source of bioactive compounds, rich in ascorbic acid, anthocyanins and phenol compounds, and have high levels of antioxidants. Strawberries have compounds that provide pigment and antioxidant activity, namely anthocyanins. This study aims to detect secondary metabolite compounds in strawberry fruit extract (Fragaria x ananassa) using Thin Layer Chromatography (TLC) with a mobile phase containing butanol, acetic acid, and aqua-distillate (4:1:1) and infrared spectrophotometry. The results of phytochemical screening tests showed that the strawberry extract contained secondary metabolite compounds, including alkaloids, flavonoids, tannins, and saponins. TLC with the Rf value in Strawberry fruit extract results in 0.38. The results of the infrared spectrophotometry analysis indicate the presence of functional groups, including C-H, CO, OH, C꓿C, and ꓿C-O-C, which corroborate the results of TLC, suggesting that the strawberry extract contains anthocyanins. Keywords: strawberry, secondary metabolite compound, TLC, infrared spectrophotometry ABSTRAK Tanaman stroberi merupakan tanaman yang banyak tumbuh di Indonesia. Tanaman stroberi memiliki banyak manfaat terutama bagian buahnya. Buah stroberi (Fragaria x ananassa) adalah sumber senyawa bioaktif, kaya akan asam askorbat, antosianin dan senyawa fenol, dan memiliki kadar antioksidan yang cukup tinggi. Buah stroberi memiliki senyawa yang memberi pigmen dan mempunyai aktivitas antioksidan yaitu antosianin. Penelitian ini bertujuan untuk mengetahui profil senyawa metabolit sekunder pada ekstrak buah stroberi dan mendeteksi senyawa antosianin pada ekstrak buah stroberi (Fragaria x ananassa) menggunakan Kromatografi Lapis Tipis (KLT) dengan fase gerak butanol-asam asetat-aquadest (4:1:1) dan FTIR. Hasil uji skrining fitokimia menunjukkan bahwa ekstrak stroberi mengandung senyawa metabolit sekunder alkaloid, flavonoid, tanin dan saponin. Hasil KLT ditunjukkan dengan nilai Rf pada ekstrak buah stroberi adalah 0,38. Hasil analisis spektrofotometri inframerah terdapat gugus fungsi O–H, C=O, C=C dan =C–O–C yang memperkuat hasil KLT bahwa ekstrak stroberi mengandung antosianin. Kata kunci: stroberi, antosianin, metabolit sekunder, KLT, FTIR
Co-Authors AA Sudharmawan, AA Abd Chalim Asnawi Afif Hilmi Ahmad Syauqi ahmad syauqi, ahmad Ainur Rofiq Ainur Rofiq Ainur Rohmah Alifia, Ersa Alik Maulidiyah Ana Li'atul Mufidah Anisa, Khoiria Ari Hayati Ari Hayati Arifyani, Laily Febrian Arinto Y.P Wardoyo Asnawi, Abd Chalim Aza Zunairoh Budi Santoso Devi Sugiarto Dewi Anjar Setyowati Dewi Rahayu Saraswati Dewi, Lina Purnama Dionysius J.D.H Santjojo Dita Agisimanto Dita Agisimanto Dita Agisimanto Dwi Budi Santoso Edi Santoso Edi Santoso Eka Prasetyowati Emeliya, Emeliya Fadhil, Ilnia Farah Aida Qotrun Nada Fitri, Hanin Rahma Garnis Mufarrohah Rohmah Garnis Mufarrohah Rohmah Gatra Ervi Jayanthi Gatra Ervi Jayanti Gatra Ervi Jayanti Gatra Ervi Jayati Gumiwang, Wulan Dari Neng Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Hasanah, Rochmatul Hesti Nofanda Hesti Nofanda, Hesti Hilmi, Afif Hosnia Sari Ilnia Fadhil Istifadah, Hayati Istiqomah, Nuri Lailatul Khoiria Anisa Lailatul Maghfiroh LAILATUL MAGHFIROH Lailatul Mufairoh Lutfi Niam Lutfi Niam, Lutfi Lutfiah sudarmaji Ma'rifatul As'adah Mai Suroh Majida Ramadhan Marista, Bilqis Mawardi, Azis Mochamad Ircham Firmansah Mufairoh, Lailatul Mufidah, Ana Liatul Muhammad Ni'amul Albab Mustakim, Moch Nada, Farah Aida Qotrun Nilam Firdausi Nilam Firdausi, Nilam Nindi Afifa Nisa Nisa, Kholisatun Nur Ainiah Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Oktavia Rahayu Puspitarini Pratiwi, Radita Intan Aisyah Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Radita Intan Aisyah Pratiwi Rafida Azizah Reza Priski Dwi Jayanti Riadul Jannah Saimul Laili Salsabilla, Dyah Ajeng Saraswati Subagyo Sari, Hosnia Setyowati, Dewi Anjar Sirojuddin M. Rochmat Siti Marhumah Siti Marhumah, Siti Siti Milatil Hasanah Siti Rahmah Suhnin, Zuhria binti Suroh, Mai Syafitri, Fitria Indah Taufiq Hidayat Ulfi Abdul Rahman Oey Vira Ruainiah Ruswandari Winda Tul Habibah Wulan Dari Neng Gumiwang Yaqutun Nafisah Yuliana Ratna Sari, Yuliana Ratna Zuhria binti Suhnin