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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Prosiding Seminar Biologi Biology, Medicine, & Natural Product Chemistry Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Sains Alami (Known Nature) Berkala Penelitian Hayati Jurnal Sains Alami (Known Nature) KOPEMAS

Tintrim Rahayu, Tintrim

Universitas Islam Malang

Author-ID : 515261

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Uji Antagonis Jamur Gliocladium sp dalam Menghambat Pertumbuhan Jamur Fusarium sp Penyebab Penyakit Layu Pada Tanaman Pisang (Musa paradisiaca L.) Taufiq Hidayat; Ahmad Syauqi; Tintrim Rahayu
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 5 No 2 (2020): Fase Pembentukan Tubuh dan Sebaran Makhluk
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.078 KB)

The fungus Gliocladium sp. is the microbial soil that can be isolated from the root zone of banana plant (Musa Paradica L.) Those fungi are included in the group of potentially antagonistic microbes in the growth inhibiting of Fusarium sp fungus which infects the plants and it cause disease on banana crops withered. This research aim was to know the difference of growth inhibiting percentage of Gliocladium sp, to the Fusarium sp. fungus at pH 5.5 and pH 7. This research uses experiment methods. The results of the growth inhibiting percentage in the last day was higher at pH 5.5 of 35.2% whereas at pH 7 of 14%. This indicates that the growth of the fungus is more suitable in pH acidic approach because of its nature which is able to adjust to the fungal metabolism. The inhibition mechanism of the Gliocladium sp. on fungus inhibiting of Fusarium sp. has the stages of the competition, lysis and mycoparasit. Keywords: Gliocladium sp., Fusarium sp, banana plants, pH ABSTRAK Jamur Gliocladium sp. merupakan mikroba tanah yang dapat diisolasi dari daerah perakaran tanaman pisang (Musa Paradica L.) Jamur tersebut termasuk dalam kelompok mikroba antagonis yang berpotensi dalam menghambat pertumbuhan jamur Fusarium sp yang menginfeksi tanaman yang menyebabkan penyakit layu pada tanaman pisang. Penelitian ini bertujuan untuk mengetahui perbedaan persentase daya hambat jamur Gliocladium sp. dalam menghambat pertumbuhan jamur Fusarium sp. pada pH 5,5 dan pH 7. Penelitian ini menggunakan metode deskriptif dan eksperimen. Hasil dari persentase daya hambat hari terakhir lebih tinggi pada pH 5,5 sebesar 35,2% sedangkan pada pH 7 sebesar 14%. Hal ini menandakan bahwa pertumbuhan jamur lebih cocok di pH mendekati asam karena sifatnya yang mampu menyesuaikan dengan metabolisme jamur. Mekanisme Penghambatan jamur Gliocladium sp. kepada jamur Fusarium sp. mempunyai tahap kompetisi, lisis dan mikoparasit. Kata kunci: Gliocladium sp., Fusarium sp., tanaman pisang, pH

Pola Pertumbuhan Pseudomonas aeruginosa untuk Retting-Embun Serat Kasar pada Tanaman Lidah Mertua (Sansevieria trifasciata) Eka Prasetyowati; Ahmad Syauqi; Tintrim Rahayu
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 5 No 1 (2019): Keragaman Populasi Makhluk
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Sansivieria trifasciata has a cell wall consisting of high cellulose components which are reinforced by lignin, pectin and hemicellulose. Separation of fibers from bonds that strengthen fibers is still a major problem because the cell walls that are owned are very strong and stiff. In the activity of separating selolusa fiber there needs to be a process called retting. Residues from the retting process with chemicals can pollute the environment, separation by mechanical means produces fibers that are still rigid, and with soaking requires a lot of water and causes odor. The purpose of this study is how long the Pseudomonas earuginosa used can show cell proliferation; get a graph of growth patterns in Sansevieria trifasciata leaf preparations by adding nutrients and fiber quality from dew retting. This study uses an experimental method; there are 2 treatments, namely the addition of nutrition and without nutrition with 6 replications time 5 days, 10, days, 15 days, 20 days and 30 days. The results of this study were the number of treated cells with the addition of nutrients and without the addition of nutrients experiencing the lag phase of the 5th to 10th day and the exponential phase starting from the 10th day to the 30th day. Pseudomonas aeruginosa can soften the cell wall with dew-retting ability obtained by 3.0 nutritional treatment scores and a score of 1.5 treatments without nutrition. In the regression analysis â‰¥ , where there is a significant influence between the number of cells per unit and the addition of nutrients and without the addition of nutrients. Keywords: growth pattern, Pseudomonas aeruginosa, coarse fiber, Sansevieria trifasciata ABSTRAK Sansivieria trifasciata memiliki dinding sel yang terdiri dari komponen selulosa tinggi yang diperkuat oleh lignin, pektin dan hemiselulosa. Pemisahan serat dari ikatan yang memperkuat serat masih menjadi masalah utama karena dinding sel yang dimiliki sangat kuat dan kaku. Dalam kegiatan pemisahan serat selolusa perlu adanya proses yang dinamakan retting. Residu dari proses retting dengan bahan kimia dapat mencemari lingkungan, pemisahan dengan cara mekanik menghasilkan serat yang masih kaku, dan dengan perendaman membutuhkan air yang cukup banyak serta menimbulkan bau. Tujuan dari penelitian ini adalah berapa lama Pseudomonas earuginosa yang digunakan dapat menunjukkan perkembangbiakan sel, mendapatkan grafik pola pertumbuhan pada sediaan daun Sansevieria trifasciata dengan penambahan nutrisi dan kualitas serat dari retting-embun. Penelitian ini menggunakan metode eksperimen, terdapat 2 perlakuan yaitu penambahan nutrisi dan tanpa nutrisi dengan 6 ulangan waktu 5 hari, 10, hari, 15 hari, 20 hari dan 30 hari. Hasil dari penelitian ini jumlah sel perlakuan dengan penambahan nutrisi dan tanpa penambahan nutrisi mengalami fase lag hari ke-5 sampai hari ke-10 dan fase eksponensial dimulai dari hari ke-10 sampai hari ke-30. Pseudomonas aeruginosa dapat melunakkan dinding sel dengan kemampuan retting-embun diperoleh skor 3,0 perlakuan nutrisi dan skor 1,5 parlakuan tanpa nutrisi. Pada analisa regresi â‰¥ , dimana adanya pengaruh yang signifikan antara jumlah sel setiap unitnya dengan penambahan nutrisi dan tanpa penambahan nutrisi. Kata kunci: Pola Pertumbuhan, Pseudomonas aeruginosa, Serat Kasar dan Sansevieria trifasciata

Kajian Etnobotani dan Reproduksi Tumbuhan Obat Di Desa Jagalan Kecamatan Kwanyar Kabupaten Bangkalan Riadul Jannah; Ari Hayati; Tintrim Rahayu
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 2 (2022): Januari 2022
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Ethnobotany is a botanical science that studies the use of plants in the needs of daily life and tribal customs. The use of plants as traditional medicine (herbal) has been known for a long time by the people in Desa Jagalan. This study aims to identify medicinal plants, utilization of medicinal plants, and reproduction of medicinal plants by the people of Jagalan Village, Kwanyar District, Bangkalan Regency. This research was conducted in May-2019 using qualitative methods. Qualitative methods are used to collect data through interviews. Respondents were selected using purposive sampling, namely community members who knew about medicinal plants, especially for parents with an age range (35-80 years). Research shows the average data of respondents encountered 150 respondents. There are 14 types of medicinal plants used in Jagalan Village, namely Bingbuluh (Belimbing wuluh), jeih (ginger). Kencur (kencor), Sere (Sirih), Molabek Temulawak), Moereng (Temuireng), Binahong, Meronggih (Kelor), Konyek (turmeric), Mores (Soursop), Jembuh (Guava), Orange Porot (Lime), Kodduk (Noni), Blimbing (Starfruit). Reproduction is divided into two parts, namely asexual and sexual, bingbuluh, merongguh, gembuh, orange porous, mores, codduk, and blimbing including the generative and vegetatf reproduction, jihor, kencor, molabek, moereng, binahong, and konyek including rhizoma reproduction (rhizome, live root) is a stem that grows horizontally in the soil resembling roots and then in sere including artificial vegetative reproduction by grafting and ducking.

Analisis Kadar Isolat Albumin Pada Daging Ikan Glodok Segar Spesies Boleophthalmus boddarti Garnis Mufarrohah Rohmah; Ari Hayati; Tintrim Rahayu
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.49 KB)

Tanaman kunyit(Curcuma) relatif mudah ditemukan dan penggunannya sudah meluas tidak hanya untuk keperluan memasak namun juga untuk kesehatan. kunyit (Curcuma) atau disebut Konyek dalam Bahasa Madura adalah tanaman yang sudah sering dimanfaatkan oleh masyarakat Desa Klabetan sebagai bahan pangan maupun obat tradisional yang sering disebut Jhemoh dalam Bahasa Madura. Secara umum minum jamu diracik dari tumbuhan telah terjadi mulai sejak turun-temurun oleh masyarakat Madura. Penelitian bertujuan untuk mengetahui persepsi masyarakat tentang pemanfaatan kunyit (Curcuma) di Desa Klabetan kecamatan Sepuluh kabupaten Bangkalan. Menggunakan metode deskriptif esploratif: studi pustaka, pengamatan di lapang, wawancara, analisis data dan dokumentasi persebaran tanaman kunyit (Curcuma) di Desa Klabetan Kecamatan Sepuluh Kabupaten Bangkalan. Hasil penelitian menunjukkan terdapat dua karakteristik kunyit yaitu kunyit (Curcuma) dengan kunyit pada pada organ sebagai bahan pangan 51% , obat obatan 46% dan ritual adat 3%. Bagian organ tanaman (Curcuma) jumlah daun 38%, rimpang 50% batang 12%. Jumlah kunyit (Curcuma) yang ditemukan sebanyak 7 titik di Dusun Bindeng dan 6 titik Dusun Bilarangan dari tiga Dusun.

In silico exploration the phenolic compound of olive leaves as acetylcholinesterase enzyme (AChE) inhibitor for Alzheimer’s disease therapy Nurul Jadid Mubarakati; Oktavia Rahayu Puspitarini; Tintrim Rahayu; Alik Maulidiyah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 2 (2019): June 2019
Publisher : The East Java Biological Society

Olive (Olea europaea) have been cultivated and grown well in tropical climates such as Indonesia. Indonesia local community have used olive as herbal medicines due to its active compounds known as oleuropein that has many biological activities including as neurotherapy in Alzheimer's disease. The purpose of this study is to explore the potential phenolic compounds of olive and examine the acetylcholinesterase (AChE) inhibitory activity displayed by different olive polyphenols through a silico approach. The bioactive compounds of olive which had been analyzed in this study were phenolic compound included oleuropein, demethyl-oleuropein, ligstroside, oleoside, verbascoside, luteolin 7-glucoside, and hydroxytyrosol. Interaction of bioactive compounds with acetylcholinesterase (AChE) was analyzed through molecular specific docking using AutoDock Vina with Pyrx Software. The result elucidate that olive contain potential biological activities as antioxidant, anti-inflammatory, antineoplastic, free radical scavenger, antibacterial, antifungal, expression TP53 enhancer, caspase 8 stimulant, platelet adhesion inhibitor, treatment for lipoprotein disorder, antiviral and dementia treatment/Alzheimer disease. The highest bioactivity percentage of olive phenolic compound are as an antioxidant of 82%, anti-inflammatory of 73%, and anti-cancer (antineoplastic) of 70% respectively. Based on molecular docking analysis show one of olive phenolic compound of the dimethyl-oleuropein has strong interaction with AChE as pointed in the binding affinity of demethyl-oleuropein +AChE of -8.9 kcal/mol has closed to galanthamine binding affinity of -10.3 kcal/mol. Along with glutamate acid 202, tyrosine 133 and tyrosine 124 are the major contributors in the target-ligand interactions. The selected demethyl-oleuropein ought to be tested in clinical studies to discover new neuro-therapeutic candidates.

Scavenging activity nano complex compounds of kelor (Moringa oleifera Lamk.) leaves and seeds Rafida Azizah; Tintrim Rahayu; Ari Hayati; Gatra Ervi Jayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 1 (2020): December 2020
Publisher : The East Java Biological Society

Moringa oleifera Lamk. is a good source of natural antioxidants because it contains various types of antioxidant compounds such as ascorbic acid, flavonoids, phenolics, and carotenoids. Those antioxidant components forming complex structure have transitional metal as central compound, which have free radical scavenging activity. This study aims to determine the active compounds that act as scavenger in leaves and seeds of M. oleifera. The possible compound found in leaves-seeds is elaborated by in silico analysis, using Dr. Duke's Phytochemical and Ethnobotanical Databases, by mean Pass online, and HitPick software. The results of in silico analysis 3 compounds identified in the leaves that had a high antioxidant role, namely beta-carotene, kaempferol, quercetin, and 2 compounds in seeds that had a high antioxidant role, namely alpha-tocopherol, beta-carotene. The results of this study indicate that the antioxidant activity of the 3 treatments had differences effectiveness of antioxidants. All of these antioxidants has ability to bind transitional metal to form free radical scavenger.

DETEKSI SENYAWA ISOFLAVON DAIDZEIN DAN GENISTEIN PADA KULTUR IN-VITRO KALUS KEDELAI (Glycine Max Merr.) Tintrim Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

The aim of this research is to identify the isofl avon compounds in the in-vitro cultured callus of soybean (Glycine max Merr.). This is an explorative research, in which callus were cultured in the B5 medium supplemented with 2 ppm 2,4 D. The friable callus were found when it was cultured in the solid medium containing 8 g/l agar and 20 g/l sucrose. When the callus and soybean were extracted with ethanol, a yellow colored substance appeared. If further analysis was done with thin layer chromatography (TLC) method employing 0,2 mm thin layer silica gel 60 F254 (DC-Plastikfolien Schicht-dicke), and eluent consisting n-Butanol - HCL 0.1 N (1:1), six light bluecolor nodes appeared under 366 nm UV light. The nodes have the following Rf: 0,14; 0,30; 0,52; 0,63; 0,79 and 0,92 respectively. This TLC result is comparable with the TLC result from soybeans since they have two nodes with the same Rf and color, namely blue color at Rf 0,81 and 0,92 respectively. Further confi rmation using HPLC (High Performance Liquid Chromatography) equipped with UVvis detector and Lichrospher 100RP–18, (10 μm) colom, as well as Hitachi D–2500 Chromato-integrator indicated that those similar two nodes identifi ed in the TLC were either daidzein or genistein. They can be detected by HPLC at 250 nm and 260 nm, when theywere eluated at the 80% metanol. The HPLC quantitative calculation indicated that concentration of daidzein is four time higher as it was compared with the daidzein concentration in the bean. The concentration of daidzein in the callus remained high up to 4–5 weeks after plantation. It’s concentration will decrease when the callus reached 6 weeks after plantation. Genistein as another component of isoflavon is not appear upon callus, while on soybean seeds extracts, both daidzen and genistein are detected.

Influence of smoking rate on ultrafine particle emission of cigarette smoke Arinto Y.P Wardoyo; Dionysius J.D.H Santjojo; Tintrim Rahayu; Saraswati Subagyo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 2 (2018): June 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/547

Ultrafine particles have been attached the attention for researchers due to their impacts on human health. Ultrafine particles can be emitted fromburning process, such as forest burning, agriculture waste burning, cigarette, etc. In this study, ultrafine particles produced by cigarette smokes hasbeen investigated as a function of smoking rate. The samples consisted of different types of Indonesia cigarette called Kretek cigarette. Thequantification of emission factors was conducted by the burning of the cigarette samples, then the smoke that was sucked with a different flow rateusing an adjustable pump. The flow rate was chosen to correspond as close as the variation of the rate that people smoke. The measurements ofultrafine concentrations were carried out using an ultrafine particle counter P-Trak TSI 8525 capable of measuring particles with the diameter in therange of 20 to 1000 nm. The results showed that the emission factor of ultrafine particles significantly depended on the smoking rate. A highersmoking rate produced higher average ultrafine particle emission factor.

Karakterisasi Bunga Tetua Anggrek Dendrobium dalam Menghasilkan Variasi Fenotipe Baru Melalui Teknik Hibridisasi Muhammad Ni'amul Albab; Tintrim Rahayu; Gatra Ervi Jayanti
Buletin Anatomi dan Fisiologi Volume 6, Nomor 2, Tahun 2021
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.6.2.2021.203-211

Peningkatan mutu pada tanaman anggrek juga dapat ditingkatkan pada teknik persilangan dan perbanyakan biji hasil persilangan. Kegiatan persilangan banyak menggunakan varietas-varietas dengan tetua yang sama sehingga menyebabkan variasi genetik pada hibrida yang terbentuk menjadi terbatas. Tujuan penelitian ini adalah untuk mendeskripsikan karakteristik bunga tetua anggrek Dendrobium yang digunakan sebagai indukan dan menganalisis bunga pada generasi hasil persilangan. Penelitian ini menggunakan metode kualitatif dengan cara mendeskripsikan karakteristik bunga tetua anggrek dengan variasi berbeda serta bunga pada anggrek hibrida hasil persilangan. Analisis dilakukan melalui pendekatan morfologi secara langsung. Hasil yang diperoleh adalah anggrek hibrida hasil persilangan yang telah berbunga memiliki morfologi bunga yang mirip seperti kedua tetua persilangannya seperti ukuran bunga, warna bunga, bentuk dan warna sepal maupun petal, dan warna dari labellum tergantung dari karakteristik bunga yang terdapat pada kedua tetua persilangannya. Kesimpulan yang didapat berupa pemilihan tetua persilangan ditentukan sesuai karakter bunga yang dikehendaki sehingga menghasilkan generasi yang memiliki variasi bunga baru dan bunga dari anggrek hibrida yang dihasilkan dari pemuliaan anggrek memiliki campuran karakteristik bunga dari kedua tetua anggrek yang digunakan sebagai indukan persilangan, tetapi tidak semua karakter bunga dari kedua tetua muncul, ada beberapa karakter bunga yang muncul dari salah satu tetua. Improving the quality of orchid plants can also be improved on the technique of crossing and seed propagation from crosses. Crossing activities use a lot of varieties with the same parents, causing genetic variation in the hybrids formed to be limited. The purpose of this study was to describe the characteristics of the flower of the Dendrobium orchid parent used as a parent and to analyze the flower in the generation of crosses. This study uses a qualitative method by describing the characteristics of the flower of elder orchids with different variations and the flowers on hybrid orchids from crosses. The analysis was carried out through a direct morphological approach. The results obtained are hybrid orchids that have flowered from crosses that have flower morphology that is similar to the two parents of the cross, such as flower size, flower color, shape and color of the sepals and petals, and the color of the labellum depending on the characteristics of the flowers found in the two parents of the cross. The conclusion obtained in the form of the selection of the crossing parent is determined according to the desired flower character to produce a generation that has new flower variations and flowers from hybrid orchids produced from orchid breeding have a mixture of floral characteristics from the two orchid elders used as cross breeders, but not all flower characters. from the two elders who appeared, several flower characters emerged from one of the elders.

PENGARUH SUHU TERHADAP AKTIVITAS ANTIOKSIDAN DALAM DAUN ZAITUN (Olea europaea L.) DENGAN METODE DPPH Ulfi Abdul Rahman Oey; Tintrim Rahayu; Gatra Ervi Jayanti
Jurnal SAINS ALAMI (Known Nature) Vol 5, No 1 (2022)
Publisher : FMIPA UNISMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/j.sa.v5i1.15927

Causes of degenerative diseases are caused by activities and unhealthy lifestyle. unhealthy lifestyle which in turn triggers free radicals. free radicals are moleculs that are not oxidized which cause the formation of new molecules that can damage body cells. free radicals can be overcome by giving natural antioxidants, one of which is form the olive plant (Olea europaea L.).The purpose of this study was to determine the effect of temperature treatment on antioxidant activity in olive leaves based on leaf age by calculating the IC50 value. The method used in this research is the DPPH method. Based on the results of the study, it can be concluded that the temperature of 31.6°c in young leaves according to calculations is categorized as a strong antioxidant because it provides a calculated value of 82.778 ppm and 165.093 ppm on old leaves and classified as moderate antioxidants, while temperature of 44°c give dominant level under 31.6°c with IC50 values 123.78 ppm for young leaves and 165.170 ppm for old leaves and classified as moderate antioxidants according to the theory that compounds that have very strong antioxidant activity values IC50 values are less than 50 ppm, strong if the IC50 are between 50 and 100, moderate antioxidant if the IC50 values are feasible 100-250 ppm, weak if the IC50 value is 250-500 ppm and inactive if the IC50 value is more than 500 ppm. and vice versa related to the selection of leaf age also affects the level of antioxidant activity seen from the comparison of results from the two treatments that young leaves have a higher level of antioxidant activity than old leaves in counteracting free radicals.Keywords : Temperature, Antioxidant, Olive (Olea europaea L.), DPPH.ABSTRAKPenyebab penyakit degeneratif disebabkan karena aktivitas dan pola hidup yang kurang sehat. Pola hidup kurang sehat yang pada akhirnya memicu radikal bebas. Radikal bebas adalah molekul yang tidak teroksidasi yang menyebabkan terbentuk molekul baru yang dapat merusak sel tubuh. Radikal bebas dapat diatasi dengan pemberian antioksidan salah satunya dari tumbuhan zaitun (Olea europaea L.). Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perlakuan suhu terhadap aktivitas antioksidan pada daun zaitun berdasarkan umur daun melalui perhitungan nilai IC50. Metode yang digunakan dalam penelitian ini adalah metode DPPH. Berdasarkan hasil penelitian dapat disimpulkan bahwa perlakuan suhu 31.6°C pada daun muda menurut perhitungan IC50 dikategorikan sebagai antioksidan kuat karena memberikan nilai hasil perhitungan IC50 sebesar 82.778 ppm dan 165.093 ppm pada daun tua dan tergolong antioksidan sedang, sementara pada perlakuan suhu 44°C memberikan pengaruh lebih rendah dibandingkan dengan perlakuan suhu 31.6°C dengan nilai IC50 sebesar 123.78 ppm untuk daun muda dan 165.170 ppm untuk daun tua dan tergolong antioksidan sedang sesuai teori bahwa senyawa yang memiliki nilai aktivitas antioksidan sangat kuat jika nilai IC50 kurang dari 50 ppm, kuat apabila nilai IC50 bernilai antara 50 sampai 100, antioksidan sedang apabila jika nilai IC50 bernilai 100-250 ppm, lemah jika nilai IC50 bernilai 250-500 ppm dan tidak aktif jika nilai IC50 bernilai lebih dari 500 ppm, begitupun sebaliknya terkait pemilihan umur daun juga berpengaruh terhadap tinggi rendahnya aktivitas antioksidan dilihat dari perbandingan hasil dari kedua perlakuan bahwa daun muda memiliki tingkat aktivitas antioksidan lebih tinggi dibandingkan dengan daun tua dalam menangkal radikal bebas.Kata Kunci : Suhu, Antioksidan, Zaitun (Olea europaea L.), DPPH.

Co-Authors AA Sudharmawan, AA Abd Chalim Asnawi Afif Hilmi Ahmad Syauqi ahmad syauqi, ahmad Ainur Rofiq Ainur Rofiq Ainur Rohmah Alifia, Ersa Alik Maulidiyah Ana Li'atul Mufidah Anisa, Khoiria Ari Hayati Ari Hayati Arifyani, Laily Febrian Arinto Y.P Wardoyo Asnawi, Abd Chalim Aza Zunairoh Budi Santoso Devi Sugiarto Dewi Anjar Setyowati Dewi Rahayu Saraswati Dewi, Lina Purnama Dionysius J.D.H Santjojo Dita Agisimanto Dita Agisimanto Dita Agisimanto Dwi Budi Santoso Edi Santoso Edi Santoso Eka Prasetyowati Emeliya, Emeliya Fadhil, Ilnia Farah Aida Qotrun Nada Fitri, Hanin Rahma Garnis Mufarrohah Rohmah Garnis Mufarrohah Rohmah Gatra Ervi Jayanthi Gatra Ervi Jayanti Gatra Ervi Jayanti Gatra Ervi Jayati Gumiwang, Wulan Dari Neng Hadi, Muhammad Sholikhul Haikal, Muhammad Wildan Hasanah, Rochmatul Hesti Nofanda Hesti Nofanda, Hesti Hilmi, Afif Hosnia Sari Ilnia Fadhil Istifadah, Hayati Istiqomah, Nuri Lailatul Khoiria Anisa LAILATUL MAGHFIROH Lailatul Maghfiroh Lailatul Mufairoh Lutfi Niam Lutfi Niam, Lutfi Lutfiah sudarmaji Ma'rifatul As'adah Mai Suroh Majida Ramadhan Marista, Bilqis Mawardi, Azis Mochamad Ircham Firmansah Mufairoh, Lailatul Mufidah, Ana Liatul Muhammad Ni'amul Albab Mustakim, Moch Nada, Farah Aida Qotrun Nilam Firdausi Nilam Firdausi, Nilam Nindi Afifa Nisa Nisa, Kholisatun Nur Ainiah Nurul Jadid Mubarokati Oey, Ulfi Abdul Rahman Oktavia Rahayu Puspitarini Pratiwi, Radita Intan Aisyah Purnamasari, Viska Puspa, Andria Putri, Nela Vede Rhofa Radita Intan Aisyah Pratiwi Rafida Azizah Reza Priski Dwi Jayanti Riadul Jannah Saimul Laili Salsabilla, Dyah Ajeng Saraswati Subagyo Sari, Hosnia Setyowati, Dewi Anjar Sirojuddin M. Rochmat Siti Marhumah Siti Marhumah, Siti Siti Milatil Hasanah Siti Rahmah Suhnin, Zuhria binti Suroh, Mai Syafitri, Fitria Indah Taufiq Hidayat Ulfi Abdul Rahman Oey Vira Ruainiah Ruswandari Winda Tul Habibah Wulan Dari Neng Gumiwang Yaqutun Nafisah Yuliana Ratna Sari, Yuliana Ratna Zuhria binti Suhnin

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