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Hendra Budi Sungkawa, Hendra Budi
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Hubungan Paparan Pestisida Dengan Kejadian Goiter Pada Petani Hortikultura Di Kecamatan Ngablak Kabupaten Magelang Sungkawa, Hendra Budi; Setiani, Onny; Suhartono, Suhartono
Jurnal Kesehatan Lingkungan Indonesia Vol 6, No 2 (2007): Oktober 2007
Publisher : Master Program of Environmental Health, Faculty of Public Health, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkli.6.2.41 - 46

Abstract

ABSTRACT Background: Pesticides are toxic material  or a substance or mixture of substances used to kill a pest or  intended for preventing, destroying, repelling or mitigating any pest.[2] A pesticide may be a chemical substance, biological agent (such as a virus or bacterium), antimicrobial, disinfectant or device used against any pest. Pests in agriculture  include insects, plant pathogens, weeds, molluscs, birds, mammals, fish, nematodes (roundworms.  Although there are benefits to the use of pesticides, there are also drawbacks, such as potential toxicity to humans and environment. Usage pesticide which do not well  managed  may  generate negative impact. Chronic poisoning of pesticides may produce adverse effect on health, including, cancer, genetic mutation, thyroid diasease, reproductive disorders and neurodegenerative disases. Preliminary researh showed that farmer in district of Ngablak   98 %  have experience of pesticide exposure. Result of study indicate that 16,5 % farmer of horticulture that have pesticide exposure  district of Ngablak  have a disorder of  thyroid and manifest as goiter. This research objective was to find out the relation between pesticides exposure and the incidence of goitre on farmer exposed to pesticides. Method: this research used a case control design with  68 case and 68 control. The variable of the research include age, education, work duration, time of activity per day, pesticide type, pesticide dose, spraying frequency, time of  spraying, farmer position to wind direction while spraying and using of personal protective equipment. Result: Research result indicated  that variables that related to the incidence of endemic goitre were age (OR = 3,83; CI 95%= 1,88 – 7,81), work duration (OR = 12, 79; CI 95% = 2,85 – 57,53), time of activity per day (OR = 2,47; CI 95% = 1,16 – 5,23), pesticide type (OR = 5,86; CI 95% = 2,73 – 12,56), pesticide dose (OR = 2,96; CI 95% = 1,37 – 6,42), spraying frequency (OR = 4,69; CI 95% = 2,28 – 9,69), farmer position to wind direction while spraying (OR = 3,07; CI 95% = 1,39 – 6,77), using of personal protective equipment (OR = 3,18; CI 95% = 1,57 – 6,41). Conclusion: Farmer’s risk factor to the incidence of endemic goitre is  working  time time of activity per day, pesticide type, spraying frequency, farmer position to wind direction while spraying  and  the use of personal protective equipmetn, in its  contribute 33,78%. Probability to the incidence of goitre on farmer exposed to pesticides. Keyword : pesticide exposure,  pesticides type, work duration, incidence of goitre, farmer
Perbedaan Kadar Protein Daging Sapi Dengan Perendaman Sari Buah Nanas (Ananas Comocus I.) Dan Sari Jahe (Zingiber Officinale Rose.) Metode Kjeldahl Sungkawa, Hendra Budi; Nurhayati, Widya; Djohan, Herlinda
Jurnal Laboratorium Khatulistiwa Vol 5, No 1 (2021): November 2021
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i1.946

Abstract

Protein merupakan asam amino rantai panjang yang diperlukan untuk memelihara jaringan, pertumbuhan dan sebagai sumber energi. Protein terdiri dari protein hewani dan nabati. Salah satu cara yang dapat digunakan untuk menaikkan pola konsumsi protein hewani, dapat menggunakan suatu enzim yaitu protease. Protease adalah salah satu enzim yang dapat digunakan untuk meningkatkan kadar protein pada bahan makanan. Nanas merupakan tanaman yang mengandung enzim protease yaitu enzim bromelin. Selain pada tanaman, enzim protease juga dimiliki oleh rempah-rempah, yaitu jahe yang dinamakan dengan enzim zingibain. Nanas dan jahe tersebut diolah menjadi sari, yang kemudian sari buah nanas dan sari jahe yang telah dibuat, direndam ke dalam bahan makanan selama waktu tertentu. Penelitian ini bertujuan untuk menjelaskan perbedaan kadar protein pada daging sapi dengan perendaman sari buah nanas (Ananas comocus 1.) dan sari jahe (Zingiber officinale Rosc. Metode penelitian yang digunakan adalah quasi experiment dengan teknik pengambilan sampel purposive sampling. Populasi dalam penelitian ini adalah daging sapi, dengan sampel daging sapi sebelum perendaman dan direndam sari buah nanas dan sari jahe sebanyak 20 ml, 30 ml, 40 ml dan lama perendaman 30 menit. Jumlah sampel yang digunakan adalah 28 sampel, dengan 7 perlakuan dan replikasi sebanyak 4 kali. Kriteria sampel yang digunakan adalah daging sapi yang di peroleh dari 4 tempat berbeda, buah nanas golongan queen yang segar dan masak, rimpang jahe putih kecil yang segar. Pengukuran sampel dilakukan dengan menggunakan metode kjeldahl. Hasil pengukuran diperoleh nilai rata-rata kadar protein sebelum perendaman dan sesudah perendaman sari buah nanas sebanyak 20 ml, 30 ml, 40 ml secara berturut-turut adalah 22,35%, 22,76%, 21,38 %, 20,86%. Hasil pengukuran nilai rata-rata kadar protein sebelum perendaman dan sesudah perendaman sari jahe sebanyak 20 ml, 30 ml, 40 ml secara berturut-turut adalah 22,35%, 20,80%, 21,82%, 20,16%. Berdasarkan uji T independent menggunakan program SPSS 26 diperoleh hasil signifikansi 0,438 > 0,05, yang menyatakan bahwa Ha ditolak, sehingga dapat disimpulkan tidak terdapat perbedaan kadar protein daging sapi dengan perendaman sari buah nanas (Ananas comocus 1.) dan sari jahe (Zingiber officinale Rosc). 
Analisis Kadar Bilangan Peroksida Pada Minyak Kelapa Yang Ditambahkan Daun Sirsak (Annona muricata Linn) Dengan Metode Iodometri manik, desima; Sungkawa, Hendra Budi; Indrawati, Ratih
Jurnal Laboratorium Khatulistiwa Vol 5, No 2 (2022): Mei 2022
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v5i2.976

Abstract

Processing of coconut oil among the people is still mostly done traditionally by heating. The oil produced has poor quality and is easily rancid. This is because heating can change the oil structure. To overcome this, we need a way that can produce quality oil with a long shelf life. Therefore, there is a need for substances that can improve the quality of oil, named antioxidants. One of the natural antioxidants that can be utilized is soursop leaves. Antioxidants in soursop leaves are acetogenin, steroids, flavonoids, glycosides, interquinone and other active substances.This study aims to analyze the effect of adding soursop leaves to the value of peroxide in coconut oil with iodometry method. The method used in this study was quasi experimental with purposive sampling. The population in this study was coconut oil. The samples in this study are coconut oil without the addition of soursop leaves and coconut oil which was added to the soursop leaves with a concentration of 1.25%, 2.5% and 5%. Based on the results of the statistical analysis of linear regression, it was found that p value = 0.038 <a 0.05 so that there was an effect of adding soursop leaves to the value of peroxide in coconut oil with a contribution of 92.5%. 
Analisis Antibakteri Sediaan Sabun Cair Ekstrak Etanol Batang Bajakah (Spatholobus littoralis Hassk) Terhadap Bakteri Staphylococcus aureus Secara In Vitro Aprisca, Meisya; Sungkawa, Hendra Budi; Wahdaniah, Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1542

Abstract

Bajakah adalah salah satu keragaman hayati yang tumbuh liar di tengah hutan, batang bajakah diketahui memiliki lebih dari satu kandungan senyawa kimia yang memiliki aktivitas farmakologi yang baik. Berdasarkan hasil pengujian fitokimia yang dilakukan pada ekstrak batang bajakah menunjukkan bahwa tumbuhan ini memiliki kandungan metabolit sekunder seperti alkaloid, flavonoid, tanin, saponin dan terpenoid yang dapat menghambat pertumbuhan bakteri sehingga dapat dimanfaatkan untuk menjadi alternative bahan pembuatan sabun cair. Tujuan dari penelitian ini adalah untuk menetapkan potensi aktivitas sediaan sabun cair ekstrak batang bajakah formula 1 (konsenstrasi 30%), formula 2 (konsentrasi 40%) dan formula 3 (konsentrasi 50%) terhadap diameter zona hambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakah metode difusi secara in vitro. Desain penelitian ini adalah (Quasi experiment). Populasi pada penelitian ini adalah sabun cair ekstrak etanol batang bajakah. Sampel pada penelitian ini adalah sabun cair ekstrak etanol batang bajakah formula 1 (konsenstrasi 30%), formula 2 (konsentrasi 40%) dan formula 3 (konsentrasi 50%) dengan 9 kali replikasi sehingga jumlah sampel yang digunakan adalah 27 sampel yang diuji kekuatannya terhadap Staphylococcus aureus. Hasil penelitian menunjukkan pada formula 1 (konsentrasi 30%) didapatkan diameter zona hambat sebesar 14,44 mm, pada formula 2 (konsentrasi 40%) didapatkan diameter zona hambat sebesar 15,22 mm, pada formula 3 (konsentrasi 50%) didapatkan diameter zona hambat sebesar 16,22 mm. Berdasarkan hasil penelitian disimpulkan bahwa pada formula 1 (konsentrasi 30%), formula 2 (konsentrasi 40%), dan formula 3 (konsentrasi 50%) adalah berpotensi kuat maka dapat disimpulkan bahwa hipotesis (H1) diterima.
Evaluasi Robustness Metode Deteksi Cepat Berbasis MPN untuk Legionella pneumophila terhadap Gangguan Mikroflora Non-Target pada Sampel Air dari Menara Pendingin Ulfia, Phisa Isyah; Sungkawa, Hendra Budi; Fatayati, Imma
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1887

Abstract

Legionellosis is an infection caused by Legionella pneumophila, commonly transmitted through artificial water systems such as cooling towers. Early detection of this pathogen is crucial, particularly in high-risk facilities. This study aimed to assess the robustness of a rapid detection method based on the Most Probable Number (MPN) principle, focusing on the effectiveness of pretreatment in reducing interference from non-target microorganisms. A total of 30 positive trays from Legiolert™ tests were subcultured on Tryptic Soy Agar (TSA) to identify any surviving non-target microbes. Robustness was defined by the proportion of trays without non-target microbial growth. Results showed a robustness rate of 93.33%, with full robustness (100%) observed in low and moderate microbial load groups, and 75% in the high-load group. A non-parametric binomial test using SPSS was performed to evaluate the statistical significance of the deviation from the robustness cut-off. The resulting p-value was 0.446 (p > 0.05), indicating no statistically significant difference. These findings demonstrate that the Legiolert™ method maintains reliable performance even in complex sample matrices, supporting its use in routine water quality monitoring in accordance with Indonesian Ministry of Health Regulation No. 2 of 2023.
Evaluation of the accuracy and precision of the microlab 300 device for total cholesterol test on the CHOD-PAP method Ananda, Fanny Rizki; Sungkawa, Hendra Budi; Kamilla, Laila; Fatayati, Imma
ELECTRON (Journal of Science and Technology) Vol. 7 No. 1 (2025): ELECTRON (Journal of Science and Technology)
Publisher : Borneo Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/electron.v7i1.516

Abstract

Internal quality assurance (PMI) is a routine preventive and monitoring activity in the laboratory to minimize errors and produce accurate tests. Quality accuracy and precision are important indicators in assessing PMI. Accuracy indicates the closeness of the test results to the true value, while precision describes the closeness of the results of repeated tests on the same sample. Total cholesterol examination using a spectrophotometer, especially the CHOD-PAP method, is often carried out at the Clinical Laboratory of the Poltekkes Kemenkes Pontianak due to its stability. This observational study aims to evaluate the accuracy and precision of the Microlab 300 tool for total cholesterol examination using the CHOD-PAP method at the Integrated Laboratory of the Poltekkes Kemenkes Pontianak. The study population was the assayed control serum solution, with samples in the form of normal control serum solution dialab which was divided into 100 vials containing 50 µL. A total of 30 vials were used for the preliminary period and 30 vials for the control period. The parameter examined was total cholesterol by CHOD-PAP method. The results showed the average accuracy of the Microlab 300 tool was 96.08% and precision was 99.21%. The daily graph using Westgard's rule shows that the control material is within the control limits. Thus, it can be concluded that the Microlab 300 tool shows good accuracy and precision in the examination of total cholesterol CHOD-PAP method in the laboratory
POTENSI JUS BAYAM MERAH (AMARANTHUS TRICOLOR L) SEBAGAI ALTERNATIF PEWARNAAN TELUR CACING SOIL TRANSMITTED HELMINTH Artanti, Lina Yunda; Sungkawa, Hendra Budi; Djohan, Herlinda; Alfianita, Riska
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 2 (2024): AGUSTUS 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i2.30402

Abstract

Cacing yang ditularkan dari tanah adalah cacing yang menginfeksi manusia melalui tanah. Identifikasi telur cacing dilakukan secara mikroskopis menggunakan teknik pewarnaan. Pewarna yang digunakan dalam memeriksa telur cacing menggunakan 2% eosin. Namun, eosin memiliki kekurangan karena tidak mudah terurai, sehingga diperlukan pewarna alternatif yang lebih ramah lingkungan. Tanaman bayam merah berpotensi untuk digunakan sebagai pewarna alami karena mengandung senyawa antosianin. Penelitian ini bertujuan untuk mengetahui potensi sari bayam merah (amaranthus tricolor L) sebagai alternatif pewarna telur cacing yang ditularkan melalui tanah. Desain penelitian eksperimen semu (quasi experiment) dengan teknik purposive sampling. Sampel yang digunakan adalah sari batang amaranthus tricolor L (amaranthus tricolor L) dengan perbandingan 1:1, 1:2, 1:3, 1:4, dan 1:5 menggunakan metode langsung dan diulang sebanyak 5 kali. Berdasarkan hasil penelitian, rasio 1:1 jus batang bayam merah menunjukkan hasil 60%, rasio 1:2 menunjukkan hasil 53%, rasio 1:3 menunjukkan hasil 53%, rasio 1:4 menunjukkan hasil 40% dan rasio 1:5 menunjukkan hasil 40%. Kesimpulan dari penelitian ini adalah konsentrasi 1:1 memiliki kualitas tertinggi yang dianggap berpotensi sebagai alternatif pewarnaan.
Pemberian Susu Kambing Prebiotik terhadap Kadar Hb pada Anak Stunting Sungkawa, Hendra Budi; sopiyandi, sopiyandi; Jaladri, Iman; R Purba, Jonni Syah; Festilia, Shelly; Suaebah, Suaebah; Mulyanita, Mulyanita
Pontianak Nutrition Journal (PNJ) Vol 7, No 1 (2024): Maret 2024
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/pnj.v7i1.1320

Abstract

Kalimantan Barat adalah provinsi di Indonesia yang memiliki masalah prevalensi stunting yang tinggi dibandingkan dengan prevalensi stunting nasional yaitu sebesar 29,8%. Kabupaten di Kalimantan Barat yang memiliki prevalensi kasus stunting yang tinggi pada tahun 2021 yaitu terdapat di Kabupaten Melawi sebesar 37,2% dan di Kabupaten Sintang sebesar 38,2%. Asupan protein menjadi faktor yang berhubungan dengan kejadian stunting. Asupan protein dapat diperoleh dari konsumsi susu setiap hari sebagai alternatif. Konsumsi jenis susu yang berasal dari hewani dapat diperoleh dari susu sapi dan susu kambing. Susu kambing lebih tidak menyebabkan alergi dan mudah dicerna dibandingkan dengan susu sapi. Susu kambing dengan penambahan probiotik pada kasus stunting dapat meningkatkan sintesis dan penyerapan mikronutrien, memediasi inflamasi, dan menyebabkan kenaikan atau penurunan berat badan, meningkatkan sensitivitas insulin, memengaruhi nafsu makan, dan meningkatkan penyerapan mineral. Jenis penelitian deskriptif analitik dengan pendekatan cohort study dimana penelitian akan dilakukan dengan membandingkan antara dua kelompok yaitu kelompok perlakuan dan kelompok control dengan rasio 1:1.  Tujuan untuk memantau perbedaan pertumbuhan anak stunting dengan asupan susu kambing dan susu kambing fortivikasi (susu kambing probiotik) di Kabupaten Kabupaten Melawi, Kalimantan Barat, yang akan dilaksanakan selama 90 hari dengan melakukan pemeriksaan darah lengkap, pemeriksaan kadar tiroid, dan kadar seng serta standar antropometri.
Potensi Air Perasan Batang Bayam Merah (Amaranthus Tricolor L) Sebagai Alternatif Pewarnaan Telur Cacing Soil Transmitted Helminth Artanti, Lina Yunda; Sungkawa, Hendra Budi; Djohan, Herlinda; Nuswantoro, Ari; Alfianita, Riska
Innovative: Journal Of Social Science Research Vol. 4 No. 4 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i4.13043

Abstract

in the examination of worm eggs uses 2% eosin. However, eosin has shortcomings because it is not easily decomposed, so an alternative coloring that is more environmentally friendly is needed. Red spinach plants have the potential to be used as a natural dye because they contain anthocyanin compounds. This study aims to determine the potential of red spinach stem juice (amaranthus tricolor L) as an alternative to staining soil transmitted helminth eggs. Research design of quasi experiment with purposive sampling technique. Samples used red spinach stem juice (amaranthus tricolor L) in a ratio of 1:1, 1:2, 1:3, 1:4, and 1:5 by direct method and repeated 5 times. Based on the results of the research, the juice of red spinach stems in the ratio of 1:1 showed 60% results, the ratio of 1:2 showed 53% results, the ratio of 1:3 showed 53% results, the ratio of 1:4 showed 40% results and the ratio of 1:5 showed 40% results. The conclusion of this study is that the concentration of 1: 1 has the highest quality which is considered to have potential as an alternative coloring.
The Effect of Contact Time Variations of Activated Carbon from Coconut Shell on the Peroxide Value in Used Cooking Oil Djohan, Herlinda; Sungkawa, Hendra Budi; Chitra, Fara; Ningsih, Nur Reza
JURNAL INFO KESEHATAN Vol 22 No 4 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss4.1856

Abstract

Cooking oil is a commonly used food product in daily life, both in households and commercially, especially for frying purposes. The repeated use of cooking oil can lead to an increase in peroxide value, which may pose health risks. High peroxide values are carcinogenic and can trigger various health problems, such as elevated cholesterol levels and heart disease. One method to reduce peroxide value is by using activated carbon, such as activated carbon derived from coconut shells. This study aims to analyze the effect of contact time variations of activated carbon from coconut shells on the reduction of peroxide value in used cooking oil. The research design used was a quasi-experiment with an iodometric testing method. A total of 24 samples of used cooking oil were treated with activated carbon from coconut shells for different contact times: 20 minutes, 30 minutes, and 40 minutes. The peroxide value was then measured. The results showed that varying the contact time with activated carbon from coconut shells resulted in the following average peroxide values: 15.57 meq O2/kg, 7.57 meq O2/kg, 6.58 meq O2/kg, and 5.82 meq O2/kg. The percentage reduction in peroxide value was 0%, 51.41%, 57.06%, and 62.64%, respectively. In conclusion, the study found a significant effect of contact time variations of activated carbon from coconut shells on the reduction of peroxide value in used cooking oil, with a probability value of 0.000 < 0.05.
Co-Authors Alfianita, Riska Ananda, Fanny Rizki Aprisca, Meisya Ari Nuswantoro Artanti, Lina Yunda Chitra, Fara Djohan, Herlinda Festilia, Shelly Imma Fatayati Indrawati, Ratih Indrawati, Ratih Jaladri, Iman Laila Kamilla manik, desima Mulyanita, Mulyanita Ningsih, Nur Reza Nurhayati, Widya Onny Setiani Pusaka, Semerdanta Qatrunada, Putri Norfa R Purba, Jonni Syah Ratnawaty, Gervacia Jenny Sopiyandi, Sopiyandi Suaebah, Suaebah Supriyanto Supriyanto Ulfia, Phisa Isyah Wahdaniah Wahyudi