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All Journal The Indonesian Biomedical Journal Paediatrica Indonesiana Acta Biochimica Indonesiana Jurnal Biomedika dan Kesehatan Majalah Kedokteran Neurosains Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia World Nutrition Journal SOSIOEDUKASI : JURNAL ILMIAH ILMU PENDIDIKAN DAN SOSIAL Muhammadiyah Journal of Geriatric Indonesian Journal of Medical Chemistry and Bioinformatics Neurona Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Ninik Mudjihartini
Department Of Biochemistry And Molecular Biology, Faculty Of Medicine, Universitas Indonesia, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Effect of intermittent fasting on fat mass and fat free mass among obese adult: A literature review Hadiyati Fudla; Ninik Mudjihartini; Helda Khusun
World Nutrition Journal Vol. 4 No. 2 (2021): Volume 04 Issue 2, August 2021
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V04.i2.0008

Abstract

Introduction Obesity is a serious hurdle facing by the world nowadays. Even though so many efforts have been done, yet the prevalence is keep rising. Intermittent fasting is seen as an effective and optimal approach for improving nutrition status without undesirable side effect. Objective to identify the effect of intermittent fasting on fat mass and fat free mass among obese adult. Methods: a literature exploration was conducted from January to October 2020 by searching the relevant studies from several databases. Results: many human clinical trials recommended that IF affects beneficial on body composition and body weight. Consuming calorie only in a certain time frame per day for 4-12 months put the body into a fast metabolism which influence the reduction of fat mass from 0.03–16.4% intervention and increasing of fat free mass for around 0.64 to 0.86%. Conclusion: intermittent fasting may reduce fat mass and increase fat free mass in obese adult through the reduction of energy intake (fasting) and the benefit to adipose tissue, liver, pancreas, skeletal muscle, and the brain.
The relationship between vitamin E and C intake with total activity of erythrocytes and breast milk superoxide dismutase in lactating mothers Ninik Mudjihartini; Diyah Eka Andayani; Sheira Taflah Putri Handana
Acta Biochimica Indonesiana Vol. 4 No. 2 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.15

Abstract

Background: Human milk contains many components, one of them is superoxide dismutase (SOD). Vitamin E and C, together with SOD, can prevent oxidative stress. Objective: This study investigated the correlation between vitamin E and vitamin C intake, with total SOD activity, in erythrocyte and breast milk among lactating mothers in Jakarta, Indonesia. Methods: Sixty lactating mothers aged 20–40 years were recruited in 1–6 months postpartum in Grogol Petamburan and the Cilincing Public Health Centre from March 2019 until April 2019. Vitamins E and C dietary intake were collected using a semi-quantitative food frequency questionnaire. SOD total activity of erythrocyte and breast milk was measured using the Ransod kit 125. Results: The median value of vitamin E intake was 6.50 mg/day, showing 91.7% of patients do not meet recommended daily intake (RDA) (19 gram/day), and the median of vitamin C intake was 120.05 mg/day with 70% participants fulfilling RDA. SOD total activity in erythrocyte and breastmilk showed a median value of 423.73 U/mL and 58.34 U/mL, respectively. The correlation between vitamin E intake with total SOD activity in erythrocyte (r = 0.143 p > 0,05) and breast milk (r = 0.041, p > 0,05) was not significant. Vitamin C intake was also not significantly correlated with SOD total activity in the erythrocyte. Conclusion: There is no significant correlation between vitamin E and vitamin C intake with the total activity of SOD of erythrocyte and breast milk in lactating mothers.
Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Abstract

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.
Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats Ninik Mudjihartini; Reni Paramita; Astrid Mariam Khairani Siregar; Estiana Filzadiyanti; Pungguri Ayu Nega Sarsanti; Erni Purwaningsih
Acta Biochimica Indonesiana Vol. 5 No. 1 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.79

Abstract

Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats. Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively. Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats. Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.
Comparison of Serum Albu-min Levels in The Breast Milk of Breastfeeding Infants Aged 1-3 Months and 4-6 Months Mudjihartini, Ninik
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 1, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstract: Background: Serum albumin is the most abundant plasma protein in the blood, contributing to maintaining colloid osmotic pressure and binding substances that are poorly soluble in plasma so that they can be distributed throughout the body. Serum albumin levels in breast milk can vary, influenced by various factors such as the lactation phase (age of the baby), number of parities, age and body mass index (BMI) of the mother. This study aims to determine the comparison of serum albumin levels in breast milk of mothers who breastfeed infants aged 1-3 months and 4-6 months and to find the relationship with the number of parities, age and BMI of the mother. Method: Cross-sectional design experimental study, breast milk samples as stored biological fluids were obtained from 58 mothers at the Petamburan and Cilincing Health Centers. Serum albumin levels were measured with the Bromocresol Green (BCG) kit. Results: The results showed that breast milk in the earlier lactation period, namely at 1-3 months, had significantly higher serum albumin levels compared to the serum albumin levels in the 4-6 months age group (p=0.002). Serum albumin levels in breast milk for infants aged 1-3 months did not correlate with mother's parity (p=0.428) and mother's age (p=0.881), but had a significant positive correlation with mother's BMI (p=000). Serum albumin levels in breast milk in the 4-6 months age group did not correlate with mother's parity (p=0.823) and mother's age (p=0.581) but had a strong positive correlation with maternal BMI (p=0.000). Conclusion: Breast milk serum albumin levels are affected by the lactation phase (age of the baby), namely at the age of 1-3 months the baby increases significantly compared to the age of 4-6 months. The level of serum albumin in breast milk is related to the mother's BMI, which increases with increasing mother's BMI.
Zinc as an inhibitor of NMDA receptor can exhibit antidepressant effect Novi Dewi Tanjung; Ninik Mudjihartini
World Nutrition Journal Vol. 6 No. 2 (2023): Volume 06 Issue 2, February 2023
Publisher : Indonesian Nutrition Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25220/WNJ.V06.i2.0006

Abstract

Background : New antidepressant strategies are needed, due to unsatisfactory clinical efficacy and many side effects of commonly used drugs. Recent studies linking the pathophysiology of depression with glutamatergic imbalance. There is hyperactivity of the main excitatory system (glutamatergic) to its inhibition (GABAergic). N-methyl D-aspartate (NMDA) receptors as a part of glutamatergic synapses are potential targets for intervention. Antagonist administration for glutamatergic systems, such as zinc, can exhibit antidepressant effects. Objective : To observe the effect of zinc administration on NMDA receptors in depressed subjects Methods : In this paper, we provide a literature review. The method to achieve the objective consists of using literature exploration, which was conducted from February to June 2022 by searching the relevant studies from several databases. Results : Study trials both in human and animal subjects reveal that depression is associated with a lower concentration of zinc. Comparison between the lowest zinc intake with the highest zinc intake had significantly lower incidence of developing depression. Dietary zinc deficiency induces depression along with upregulation of the NMDA receptor complexes. Zinc’s antidepressant effects might be mediated through its action reducing NMDA channel-opening frequency. Conclusions : The presence of zinc may downregulate the glutamate response in binding to NMDA receptors. Because of numerous studies about the connection between zinc and depression, it seems that zinc may have the potency to develop new antidepressants. Since the capability of zinc administration to reduce depressive symptoms, it is expected leading to increased medication adherence, lower costs and better outcomes.
Kadar Neuroglobin dan Sitoglobin dalam Plasma, Cairan Serebro Spinalis, dan Jaringan Otak Pasien Strok Hemoragik Mudjihartini, Ninik; Saekhu, Mohamad; Jusman, Sri Widia A.; Sadikin, Mohamad
Muhammadiyah Journal of Geriatric Vol 3, No 1 (2022): Muhammadiyah Journal of Geriatric
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mujg.3.1.1-8

Abstract

Latar belakang: Otak memerlukan oksigen yang banyak selain glukosa. Hipoksia iskemik karena strok hemoragik atau Spontaneous intracerebral hemorrhage (sICH) dapat mengganggu suplai oksigen dan nutrisi ke otak berakibat produksi energi di otak akan menurun. Deplesi energi ini menyebabkan kerusakan dan kematian sel otak terjadi lebih cepat. Neuroglobin (Ngb) dan sitoglobin (Cygb) merupakan protein golongan globin yang terdapat di otak dan berperan sebagai protein pengikat oksigen di mitokondria. Tujuan: Penelitian ini bertujuan mendapatkan gambaran/profil kadar Ngb dan Cygb, di plasma, cairan serebro spinal (CSS), dan jaringan otak pasien strok hemoragik. Metode: Penelitian ini merupakan penelitian lanjutan menggunakan sampel plasma, CSS, dan jaringan otak yang diperoleh saat kraniotomi evakuasi hematoma pasien strok hemoragik sICH di rumah sakit Cipto Mangunkusumo dan rumah sakit lainnya di Jakarta. Kadar protein Ngb dan Cygb dari plasma, CSS, dan jaringan otak diukur dengan metode ELISA. Hasil: Rerata kadar Ngb otak adalah 0,058 ng/mg protein otak, sedangkan di plasma dan CSS masing-masing adalah 0,017 ng/mg protein otak dan 0,013 ng/mg protein otak atau 29,31% dan 22,41% dari rerata kadar Ngb otak. Rerata kadar Cygb otak adalah 4,943 ng/mg protein otak, di CSS adalah 1,685 ng/mg protein otak, atau 25,26% dari rerata Cygb otak, sedangkan di dalam plasma hampir tidak terdeteksi. Simpulan: Pada keadaan hipoksia oleh karena strok hemoragik sICH, protein Ngb dan Cygb dapat diukur di plasma, CSS, dan jaringan otaknya.
Efek Hipoksia Sistemik Kronik Terhadap Aktivitas Spesifik Enzim Kreatin Kinase Otot Rangka Tikus Mudjihartini, Ninik; Harmelia, Dwi; Widia AJ, Sri
Muhammadiyah Journal of Geriatric Vol 4, No 1 (2023): Muhammadiyah Journal of Geriatric
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mujg.4.1.1-9

Abstract

Latar Belakang: Hipoksia adalah hasil dari ketidakseimbangan antara suplai oksigen dan kebutuhan oksigen. Pada kondisi hipoksia, otot rangka dituntut untuk menghasilkan ATP dalam jumlah yang cukup untuk mempertahankan fungsi kontraksi dengan O2 yang terbatas. Beberapa penyakit terkait penuaan sering disertai dengan kondisi hipoksia, demikian juga penyakit yang disebabkan oleh kelainan jantung dan paru. Hipoksia kronik sering terjadi pada populasi lanjut usia oleh karena cedera iskemik yang menginduksi sejumlah respons adaptif terhadap lingkungan mikro selnya. Tujuan: Penelitian ini untuk melihat bagaimana fenomena perubahan metabolisme salah satu sumber energi pada otot rangka pada kedaan hipoksia sistemik kronik, dengan mengukur aktivitas spesifik CK dan kadar kreatinin otot rangka pada tikus. Metode: Tikus hipoksia dibuat dengan cara memasukkan tikus ke dalam hypoxia chamber yang dialiri campuran gas oksigen 10% dan nitrogen 90%. Aktivitas spesifik CK diukur secara spektrofotometri menggunakan kit Creatine kinase N-acetyl-L-cysteine (Randox®), kadar kreatinin dengan metode Folin, sedangkan protein total otot tikus diukur dengan metode Christian-Warburg. Hasil: Aktivitas spesifik CK otot tikus kelompok 1H, 3H, 5H, 7H, dan 14H menurun secara bermakna dibandingkan dengan kelompok K (Kruskal-Wallis) (P0,05). Kadar kreatinin otot tikus kelompok 1H dan 14H menurun secara bermakna dibanding dengan kelompok K, sedangkan kelompok 3H, 5H, 7H  meningkat secara bermakna dibanding dengan kelompok K (Kruskal-Wallis) (P0,05). Simpulan: Hipoksia sistemik kronik menyebabkan penurunan aktivitas spesifik CK otot tikus semua kelompok dibanding kontrol. ATP untuk membentuk kreatin fosfat menurun menyebabkan kreatin bebas meningkat. Hasil oksidasi kreatin bebas menjadi kreatinin meningkat terlihat pada kelompok 3H, 5H, dan 7H.
Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta Iswanti, Febriana Catur; Mudjihartini, Ninik; Paramita, Reni; Purwosunu, Yuditiya; Prijanti, Ani Retno
Acta Biochimica Indonesiana Vol. 7 No. 2 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.179

Abstract

Background: Hypertensive disorders, particularly preeclampsia, are major contributors to maternal mortality and neonatal morbidity. Angiogenic imbalance plays a critical role in placental ischemia, a hallmark of preeclampsia. Purpose: This study aimed to investigate the roles of endoglin, soluble endoglin (sEndoglin), and matrix metalloproteinase-14 (MMP-14) in the angiogenic imbalance observed in preeclampsia placentas compared to normal-term placentas. Method: A cross-sectional study was conducted using 68 placental samples: 34 from normal-term pregnancies and 34 from preeclampsia cases. Concentrations of endoglin, sEndoglin, and MMP-14 were measured using the sandwich ELISA method, and protein levels were determined using the Christian Warburg method. Data were analyzed using SPSS version 20. Results: The concentration of endoglin in preeclampsia placentas was slightly higher (1.37 [0.2–2.2] ng/μg protein) compared to normal placentas (1.12 [0.6–14.1] ng/μg protein), although the difference was not statistically significant. In contrast, sEndoglin (0.05 [0.0–0.01] ng/μg protein vs. 0.02 [0.0–0.3] ng/μg protein) and MMP-14 (0.14 [0.1–0.6] ng/μg protein vs. 0.11 [0.1–1.3] ng/μg protein) concentrations were significantly higher in preeclampsia placentas compared to normal placentas. All parameters showed a gradual decrease with advancing gestational age. sEndoglin and MMP-14 demonstrated a strong positive correlation (r = 0.658, p < 0.001), while endoglin and MMP-14 exhibited a moderate positive correlation (r = 0.554, p < 0.001). Conclusion: Endoglin, sEndoglin, and MMP-14 were differentially expressed in preeclampsia placentas, with sEndoglin and MMP-14 significantly elevated. These findings highlight their potential role in angiogenic imbalance and may provide insight into the pathophysiology of preeclampsia.
Hubungan Kadar Plasma Leptin dan Fungsi Kognitif pada Lanjut Usia di Jakarta Pratiwi, Sandi Puspita; Mudjihartini, Ninik; Lestari, Wiji; Prawirohajo, Pukovisa
Majalah Kedokteran Neurosains Perhimpunan Dokter Spesialis Saraf Indonesia Vol 40 No 3 (2024): Volume 40, No 3 - Juni 2024
Publisher : PERDOSNI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52386/neurona.v40i3.544

Abstract

Introduction: The increasing of elderly population often accopanied by a rise in cognitive disorders. Leptin is known to have a protective function against cognitive dectine in elderly. However, current finding regarding the role of leptin in cognitive function is vary, and has not been extensively discussed in Indonesia. Food record and antropometric assesment is conducted as an aditional analyses on subject. Aim: To determine the relationship between plasma leptin levels and cognitive function in the elderly in Jakarta. Methods: This is a cross sectional descriptive analytical research using purposive sampling as the sampling method. Subject are elderly residing in social welfare home, and being assessed using MOCA-INA instrument, IADL and GPAQ questionnaire, plasma leptin, body composition, antropometric and Food Record are measured. The data is analyzed through univariate, bivariate and multivariate analysis. Results: Subject characteristic of age ranging from 60-74 years, with female as the majority (67.5%), normal nutritional status (46.2%), body fat ranging from 18.10 to 57.10 %, muscele mass 20.20 to 57.50 kg. Majority of subject do not smoke, have a low education level (≤ 12 year of education), engage in moderate physical activity, and having independent functional capacity. A total of 96.2% of the subject experience cognitive impairment. Plasma leptin levels ranging from 1.4 to 8.5 ng/mL, with median of 5.9 ng/mL. There is a significant relationship between leptin levels, body mass index, body weight, total body fat, education and IADL with cognitive function in the elderly in Jakarta. Discussion: The correlation between plasma leptin levels and cognitive function in the elderly in Jakarta, shows a moderately positive correlation with r value of 0.47 and a significance of 0.000. Keywords: Cognitive function, Elderly, Leptin
Co-Authors Abd. Rasyid Syamsuri Achmad Mulawarman Jayusman Ahmad A. Jusuf Ahmad Aulia Almira Devina Gunawan Andriani Andriani Ani Retno Prijanti Astrid Mariam Khairani Siregar Chandra, Dian Novita claresta diella Devana, Natasha Dianasari Diana Sunardi Diyah Eka Andayani Diyah Eka Andayani Ekawati, Maria Endrico Xavierees Erni Purwaningsih Estiana Filzadiyanti Febriana Catur Iswanti Hadiyati Fudla Hajar, Siti Sarah Harmelia, Dwi Helena Fabiani Ika Superti Daruningrum Inge Permadhi Jusman, Sri Widia Azraki Karin Wiradarma Khusun, Helda Krisnamurti, Desak G.B. Livia Kurniati Saputra Mohamad Sadikin Mohamad Sadikin Mohamad Saekhu Muchtaruddin Mansyur Mulyana Mulyana Nani Dharmasetiawani Novi Dewi Tanjung Nurdina, Nazlah Nurhadi Ibrahim Nurul Ratna Mutu Manikam Pratiwi, Issabella Nisrina Pratiwi, Sandi Puspita Prawirohajo, Pukovisa Pungguri Ayu Nega Sarsanti Purwaningsih, Erni H. Purwosunu, Yuditiya Rahmawaty Ridwan, Rahmawaty Raphael Kosasih Ratna, Nurul Ray Sugianto, Ray Reni Paramita Reni Paramita Ria Andreinie Rini Sekartini Rita Lahirin, Rita Sadikin, Abdul Halim Saptawati Bardosono Saptawati Bardosono Setyarini, Indah Bachti Sheira Taflah Putri Handana Sheira Taflah Putri Handana Sianipar, Imelda M. Siti Farida Sri W.A. Jusman Sri Widia A Jusman Wahyu Ika Wardhani Wawan Mulyawan Widia AJ, Sri Wiji Lestari Wiji Lestari Wiwoho, Yudi Yuwono Yulhasri Yulhasri