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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar AL KAUNIYAH BERKALA SAINSTEK REKAYASA UNEJ e-Proceeding REINWARDTIA Jurnal Biodjati BIOEDUKASI Jurnal Malikussaleh Mengabdi Life Science and Biotechnology Biosaintifika: Journal of Biology & Biology Education Reinwardtia Jurnal Penelitian Sains dan Teknologi Indonesia
Fuad Bahrul Ulum
Jurusan Biologi, FMIPA, Universitas Jember, Jl. Kalimantan No.37, Jember, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Journal : Life Science and Biotechnology

 1 
Epiphytic ferns and orchids adaptation mechanism based on stomatal structure and chlorophyll content in Urban Forest of Jember University, Indonesia Ulum, Fuad bahrul; Setyati, Dwi; Su'udi, M.
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39678

Abstract

Epiphytic orchids and ferns in urban forests simulating a lowland tropical forest ecology were studied. This study aims to provide information about the epiphytes' adaptation to living in a dynamic microclimate of the tree canopy. Five epiphytes from the two taxa (orchids and ferns) were observed in their stomatal structure and chlorophyll contents. The stomatal type, size, and density were analysed with microscopic observation. The chlorophyll content was measured using a spectrophotometer. The orchids developed different mechanisms for water conservation compared to the ferns. Orchis develop smaller sizes and denser stomata to support photosynthesis rate as they had water absorbers and storage organs. On the other hand, ferns tend to avoid drought stress by developing fewer numbers and sparse stomata. The higher chlorophyll content in ferns was for photosynthesis efficiency purposes
The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids Su'udi, Mukhamad; Setyati, Dwi; Khoirunnisa, Gita Ayu; Afidah, Yusfi; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39851

Abstract

matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.
Poiploidy induction of Indonesian Black Rice Oryza sativa L. Var. Cempo Ireng with Bio-catharantine Kurniawan, Ludfi; Laili, Alvina Nur; Angraeni, Devi Silvia; ‘Ain, Salsabila Qurrotu; Wulandari, Dyah Retno; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43753

Abstract

High demand on black rice Oryza sativa L. due to their high antioxydant content and better nutrition than the white rice. On the other hand this high price rice still less interest for the farmet to cultivate it. Therefore, improvement of plant characters was carried out through polyploid induction using the natural anti-mitotic compound bio-catharanthine. The purpose of this study was to test the effectiveness of bio-catharanthine in inducing polyploidy in Cemp ireng black rice. This study used a two-factor completely randomized design (CRD) method with three replications. The treatment factor of bio-catharanthine concentration was 1%, 1.5 %, 2 %, 2 %, 2.5 % and 3 % with the soaking time of 48 hours. The germination rate, ploidy level, stomatal size and density, and antioxidant were measuret to observed the characteristic of the mutan. The results showed that the treatments of 3 % bio-catharanthine enhanced the chromosome number of black rice Cempo ireng. Biocatharantine did not affected the germination rate through the treatments. Out mutants shows alternation on the stomatal size and density. The antioxidant of leaf sample did not changed after the treatment. Bio-catharanthine on high concentration migh be possible to be applied on polyploidy induction in black rice
In Silico Study of Genus Marchantia using matK loci for DNA Barcoding Farhan, Abdillah Maulana; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53527

Abstract

Marchantia is a genus of liverworts that can be found in the Asia-Europe Region. Some of the types that can be found are Marchantia polymorpha subsp. montivagans, Marchantia polymorpha subsp. ruderalis, Marchantia paleacea subsp. diptera. All of these species have different levels of kinship, this is due to differences in their nucleotide structure. The purpose of this study was to identify the intolerance between Marchantia species in genomic studies using the NCBI Database. The method used in this research is the collection of nucleotides on NCBI and then construction with Mega 11 software. The marker used in this study is Maturase K (matK), this is because the marker is very optimal in the identification of a molecular-based species. The results of this study are Marchantia paleacea subsp. diptera matK (MP 58 and MP 54) has the highest level of kinship with a score of 99 (Neighbor-Joining Tree) and 100 (Maximum Likelihood Tree) and the outgroup of this phylogenetic is Dumortiera hirsuta. Keywords: Marchantia, matK, NCBI, Phylogenetic.
In Silico Biological Activity Prediction of Bioactive Compounds from Dumortiera hirsuta (Sw.) Nees. Using Way2Drug PASS Online Farhan, Abdillah Maulana; Rosida, Waki'atil; Ulum, Fuad Bahrul
Life Science and Biotechnology Vol. 3 No. 1 (2025): May 2025
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

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Abstract

The liverwort Dumortiera hirsuta (Sw.) Nees. is recognized as a potential source of pharmacologically active metabolites. Bioactive compounds from D. hirsuta have been previously reported through in vitro metabolomic analyses using gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS). In this study, the biological activities of the GC-MS-identified metabolites were evaluated in silico using the Way2Drug PASS Online platform. The results indicated that ten metabolites from D. hirsuta exhibit medicinal potential, with Pa values greater than 0.7, suggesting a high probability of biological activity. Among these compounds, stigmasterol (Pa = 0.970) and lathosterol (Pa = 0.960) demonstrated the strongest antihypercholesterolemic potential, indicating their role as natural agents for reducing cholesterol levels. These findings highlight the pharmacological potential of D. hirsuta metabolites and warrant further validation through in vitro cholesterol-lowering assays to confirm their predicted activities.
Phylogenetic Analysis of Manihot sp. Based on matK and rbcL Markers Rosida, Wakiatil; Ulum, Fuad bahrul; Su'udi, Mukhamad
Life Science and Biotechnology Vol. 3 No. 1 (2025): May 2025
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

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Abstract

Cassava (Manihot sp.) is a staple crop of major economic and ethnomedicinal importance. Understanding the phylogenetic relationships within the genus Manihot is essential for clarifying its taxonomy and supporting genetic improvement programs. This study aimed to reconstruct the phylogeny of several Manihot species using two chloroplast DNA markers, matK and rbcL. Sequence data were retrieved from the GenBank database, and Ricinus communis was designated as the outgroup. Phylogenetic analyses were performed using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods, each with 1,000 bootstrap replications to assess branch support. The resulting trees consistently clustered Manihot esculenta within a well-supported monophyletic group, indicating a close genetic relationship with other congeners. Distinct clades corresponding to genetic similarity were also observed among the analyzed species. These findings enhance current knowledge of Manihot systematics, providing molecular evidence that supports its taxonomic classification. Furthermore, the results contribute valuable insights for cassava breeding and conservation efforts through improved understanding of its evolutionary relationships.
Co-Authors Afiatri Putrika AFIATRI PUTRIKA AFIATRI PUTRIKA, AFIATRI Afidah, Yusfi Angraeni, Devi Silvia Ardiyansah, Muhammad Arif Mohammad Siddiq Arsetyo Rahardianto ‘Ain, Salsabila Qurrotu DIAN APRIANI DIAN APRIANI DWI INDAHNING ROHMAH Dwi Setyati Dwinianti, Edia Fitri Eny Yuniati Fadri, Firda FADZILAH AG FADZILAH AG. KANAK FADZILAH AG. KANAK, FADZILAH AG. Farhan, Abdillah Maulana Fitri, Edia Fitri, Nurfajri Eka Gilani, Noor Azizah Hayyinah, Asa Hanifatul Hidayat Teguh Wiyono Hidayatullah, Alhabsy Indah Wahyuni INDAH WAHYUNI KANAK KANJANA WONGKUNA KANJANA WONGKUNA KANJANA WONGKUNA, KANJANA Khoirunnisa, Gita Ayu Kurniawan, Ludfi Laili, Alvina Nur LESLEY C. LUBOS LESLEY C. LUBOS LESLEY C. LUBOS, LESLEY C. LUONG THIEN TAM LUONG THIEN TAM LUONG THIEN TAM, LUONG THIEN Miatin Alvin Septianasari Mika Rizki Puspaningrum MIKA RIZKI PUSPANINGRUM MIKA RIZKI PUSPANINGRUM, MIKA RIZKI MOHD RAWIYANI PG. HJ. SERUDIN MOHD RAWIYANI PG. HJ. SERUDIN MOHD RAWIYANI PG. HJ. SERUDIN, MOHD RAWIYANI PG. MONICA SULEIMAN MONICA SULEIMAN MONICA SULEIMAN, MONICA Mudinillah, Adam Mukhamad Su'udi Musyarofah Zuhri MUSYAROFAH ZUHRI Musyarofah Zuhri NG AIK MIN NG AIK MIN NG AIK MIN, NG AIK NURLISMA JUNITA NURLISMA JUNITA NURLISMA JUNITA, NURLISMA Nursahara Pasaribu NURSAHARA PASARIBU Nursahara Pasaribu Ratnasari, Tri Rendy Setiawan Rizqoni, Moh Ikmal Alfi ROBBERT GRADSTEIN ROBBERT GRADSTEIN ROBBERT GRADSTEIN, ROBBERT Rosida, Waki'atil Rosida, Wakiatil SATRIYAS ILYAS SOONTHREE KORNOCHALERT SOONTHREE KORNOCHALERT SOONTHREE KORNOCHALERT, SOONTHREE Su'udi, M. Su'udi, Mukhamad Sudarmadji, S. Sulistiyowati, Hari Su’udi, Mukhamad Ulaa, Munaa Aqidatul Veren Yuliana Saputri Wulandari, Dyah Retno YONG KIEN–THAI, YONG YONG KIEN–THAI YONG KIEN–THAI