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All Journal Journal of Midwifery and Nursing Best Journal (Biology Education, Sains and Technology) Jurnal Penelitian Farmasi dan Herbal International Journal Of Science, Technology & Management (IJSTM) Jurnal EduHealth FORTE JOURNAL JIFI (Jurnal Ilmiah Farmasi Imelda) Jurnal Pengabdian Masyarakat Putri Hijau Sustainable Applied Modification Evidence Community Science Get Journal
Harnis, Zola Efa
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Exploration of CRISPR-Cas9 Gene Editing System Utilization for Targeted Induction of Apoptosis in Cancer Cells Resti Ariani; Eka Cahya Muliawati; Zola Efa Harnis
Science Journal Get Press Vol 3 No 1 (2026): January, 2026
Publisher : CV. Get Press Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69855/science.v3i1.436

Abstract

The CRISPR-Cas9 gene editing system represents a powerful approach for precision targeting of genetic determinants involved in cancer cell survival. This study evaluated the ability of CRISPR-Cas9 to induce apoptosis in cancer cells through targeted disruption of anti-apoptotic genes. Acute myeloid leukemia (HL-60 and KG-1) and anaplastic thyroid cancer (8505C and C643) cell lines were used to knockout BIRC5 (survivin) and MADD using specific single-guide RNAs. Efficient genome editing was achieved, with indel formation exceeding 50% across all models. Targeting BIRC5 and MADD significantly reduced cell viability to 39.8–54.6% of control levels and induced substantial apoptosis. Total apoptotic populations reached 52.8–60.1% following BIRC5 knockout and 45.3–50.6% following MADD knockout. Apoptosis induction was confirmed by caspase-3/7 activation, increased Sub-G1 accumulation, and cleavage of apoptosis-related proteins, indicating activation of the intrinsic apoptotic pathway.Notably, BIRC5 disruption consistently produced stronger pro-apoptotic effects than MADD across all cancer models. Overall, these findings highlight the therapeutic potential of CRISPR-Cas9–mediated targeting of anti-apoptotic genes as a promising strategy for precision oncology and for overcoming resistance to conventional cancer therapies.
Co-Authors amar, ageng azhar Anggun Syahfitri ASWAN PANGONDIAN Athaillah, Athaillah Ayu Meta Sari Br. Perangin-angin Bunga Rimta Barus Chindy Umaya Delisma Marsauli Simorangkir Eka Cahya Muliawati Facrunisa, Dian Farcrunisa, Dian Facrunisa Fitrhyyah, Hazimah Fitrhyyah Harahap, Nina Irmayanti Harahap, Nina Irmayanti Harahap Hayati, Silmi Hayati, Silmi Hayati Irmayanti Harahap, Nina Jepanya Tio Maranantha Bangun Lilik Septiana Mardatillah, Hazimah Fithryyah Melawatito Sitanggang Murdiani Naibaho, Legawati Nina Irmayanti Harahap Nina Irmayanti Harahap Nina Irmayanti Harahap Ovalina Sylvia Br. Ginting Palas Tarigan Purba, Jhan Saberlan Puspita, Rika Putri Andini Resti Ariani Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rissa Dewi Ramadhani Saberlan, Jhan Saberlan Saddam Husein Sarviana Lubis Septi Viantri Kurdaningsih Silmi Hayati Simorangkir, Delisma Simorangkir Sofia Eliasari Br Bangun Sri Rezeky sulasmi sulasmi Syafitri, Anggun Syafitri, Anggun Syafitri Sylvia, Sylvia Tamba, Agnes Clara Zul Azhar Safruna