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All Journal Journal of Midwifery and Nursing Best Journal (Biology Education, Sains and Technology) Jurnal Penelitian Farmasi dan Herbal International Journal Of Science, Technology & Management (IJSTM) Jurnal EduHealth FORTE JOURNAL JIFI (Jurnal Ilmiah Farmasi Imelda) Jurnal Pengabdian Masyarakat Putri Hijau Sustainable Applied Modification Evidence Community Science Get Journal
Harnis, Zola Efa
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Phytochemical Screening and Formulation of Hand Sanitizer Gel Preparations from Temu Hitam Rhizome Extract (Curcuma aeruginosa Roxb) Sari, Rika Puspita; Efa Harnis, Zola; Irmayanti Harahap, Nina
International Journal of Science, Technology & Management Vol. 3 No. 6 (2022): November 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i6.684

Abstract

Temu Hitam (Curcuma aeruginosa Roxb) is one of the most widely grown plants in Indonesia at an altitude of 400–750 m asl and contains many secondary metabolites, including flavonoids, alkaloids, saponins, tannins, and curcuminoids, which in the test had antibacterial activity and growth inhibitors. worm larvae in the gastrointestinal tract and the effect of increasing the body's immunity The purpose of this study was to identify the content of secondary metabolites contained in the rhizome of black turmeric, which would then be formulated into gel hand sanitizer preparations. Secondary metabolite testing was conducted on alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides. Three different hand sanitizer gel formulations were created using different extract concentrations, 5%, 10%, and 15%. Evaluation tests were then run on the preparations, including stability, organoleptic, homogeneity, pH, and dispersion tests. Alkaloids, flavonoids, tannins, saponins, and steroids were found in the temu hitam rhizome, according to the results of secondary metabolite testing. The dosage form was semi-solid, had a light brown to blackish brown hue, a distinct spice scent, was homogeneously prepared, and had a pH of 7.0, according to the examination of the formulation results 4,1 ± 0,17; 4,4 ± 0,19; 4,9 ± 0,08; 4,9 ± 0,09, viscosity 2378 ± 209,9; 3340 ± 220,7; 4509 ± 285,6; 4881 ± 276,7; and spreadability test 5; 6,2; 5,4; 4,9 cm . The conclusion of this study is that the results of the phytochemical screening of simplicia and the ethanolic extract of the Temu Hitam rhizome showed positive results, containing secondary metabolites of alkaloids, flavonoids, tannins, saponins, glycosides, and steroids/triterpenoids that can be formulated into hand sanitizer gel preparations that meet the requirements for preparation evaluation.
Exploration of CRISPR-Cas9 Gene Editing System Utilization for Targeted Induction of Apoptosis in Cancer Cells Resti Ariani; Eka Cahya Muliawati; Zola Efa Harnis
Science Journal Get Press Vol 3 No 1 (2026): January, 2026
Publisher : CV. Get Press Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69855/science.v3i1.436

Abstract

The CRISPR-Cas9 gene editing system represents a powerful approach for precision targeting of genetic determinants involved in cancer cell survival. This study evaluated the ability of CRISPR-Cas9 to induce apoptosis in cancer cells through targeted disruption of anti-apoptotic genes. Acute myeloid leukemia (HL-60 and KG-1) and anaplastic thyroid cancer (8505C and C643) cell lines were used to knockout BIRC5 (survivin) and MADD using specific single-guide RNAs. Efficient genome editing was achieved, with indel formation exceeding 50% across all models. Targeting BIRC5 and MADD significantly reduced cell viability to 39.8–54.6% of control levels and induced substantial apoptosis. Total apoptotic populations reached 52.8–60.1% following BIRC5 knockout and 45.3–50.6% following MADD knockout. Apoptosis induction was confirmed by caspase-3/7 activation, increased Sub-G1 accumulation, and cleavage of apoptosis-related proteins, indicating activation of the intrinsic apoptotic pathway.Notably, BIRC5 disruption consistently produced stronger pro-apoptotic effects than MADD across all cancer models. Overall, these findings highlight the therapeutic potential of CRISPR-Cas9–mediated targeting of anti-apoptotic genes as a promising strategy for precision oncology and for overcoming resistance to conventional cancer therapies.
Co-Authors amar, ageng azhar Anggun Syahfitri ASWAN PANGONDIAN Athaillah, Athaillah Ayu Meta Sari Br. Perangin-angin Bunga Rimta Barus Chindy Umaya Delisma Marsauli Simorangkir Eka Cahya Muliawati Facrunisa, Dian Farcrunisa, Dian Facrunisa Fitrhyyah, Hazimah Fitrhyyah Harahap, Nina Irmayanti Harahap, Nina Irmayanti Harahap Hayati, Silmi Hayati, Silmi Hayati Irmayanti Harahap, Nina Jepanya Tio Maranantha Bangun Lilik Septiana Mardatillah, Hazimah Fithryyah Melawatito Sitanggang Murdiani Naibaho, Legawati Nina Irmayanti Harahap Nina Irmayanti Harahap Nina Irmayanti Harahap Ovalina Sylvia Br. Ginting Palas Tarigan Purba, Jhan Saberlan Puspita, Rika Putri Andini Resti Ariani Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rika Puspita Sari Rissa Dewi Ramadhani Saberlan, Jhan Saberlan Saddam Husein Sarviana Lubis Septi Viantri Kurdaningsih Silmi Hayati Simorangkir, Delisma Simorangkir Sofia Eliasari Br Bangun Sri Rezeky sulasmi sulasmi Syafitri, Anggun Syafitri, Anggun Syafitri Sylvia, Sylvia Tamba, Agnes Clara Zul Azhar Safruna