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PENGARUH EKSTRAK ETANOL DAUN GAMBIR (UNCARIA GAMBIR) DIBANDINGKAN DENGAN CHLORHEXIDINE GLUCONATE 0,2% TOPIKAL TERHADAP PENYEMBUHAN LUKA MUKOSA PALATUM TIKUS GALUR WISTAR: EFFECT OF ETHANOL GAMBIR LEAVES (UNCARIA GAMBIR) COMPARED WITH CHLORHEXIDINE GLUCONATE 0,2% TOPICAL FOR WOUND HEALING ON PALATE MUCOSAL GALUR WISTAR RAT Dita Septiani; Euis Reni Yuslianti; Saskia Lenggogeni Nasroen
Dentika: Dental Journal Vol. 18 No. 3 (2015): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.945 KB) | DOI: 10.32734/dentika.v18i3.1973

Abstract

Gambir (Uncaria gambir) digunakan masyarakat secara empiris dalam membantu penyembuhan luka karena memilikikemampuan antibakteri, antiinflamasi dan antioksidan. Penelitian ini bertujuan mengetahui pengaruh ekstrak etanol daungambir topikal terhadap penyembuhan luka mukosa palatum tikus galur wistar dibandingkan chlorhexidine gluconate0,2% ditinjau dari luas permukaan luka dan lama penyembuhan luka serta mengetahui dosis efektif ekstrak etanol daungambir secara topikal untuk penyembuhan luka optimal. Metode penelitian menggunakan rancangan penelitianeksperimental murni laboratoris bersifat analitik. Tikus dibagi acak menjadi tujuh kelompok yaitu kelompok kontrolnegatif, kelompok chlorexidine gluconate 0,2%, kelompok ekstrak etanol daun gambir dosis 1,75 mg, 3,5 mg, 7 mg, 14mg dan 28 mg. Luka diamati hari ke-0, 3, 7 dan 14. Hasil penelitian menunjukkan ekstrak etanol daun gambirberpengaruh terhadap penyembuhan luka berhubungan dengan kandungan daun gambir yaitu katekin, asamcathechutannat, quersetin, tanin, alkaloid, saponin dan gambiriin berperan sebagai antibakteri, antiinflamasi danantioksidan. Hasil tes statistik luas permukaan luka dengan uji Kruskal-Wallis menunjukkan nilai p=0,00 dan hasil tesstatistik efektivitas dosis dengan uji Friedman menunjukkan nilai p=0,00. Sebagai kesimpulan, ekstrak etanol daungambir dapat lebih cepat memperkecil luas dan lama penyembuhan luka dibandingkan chlorhexidine gluconate 0,2%serta didapatkan dosis efektif yaitu 28 mg.
Aplikasi gel serbuk membran telur ayam ras 10% (Gallus gallus domesticus) terhadap penyembuhan luka sayat gingiva tikus galur WistarEffect of 10% chicken egg membrane gel powder (Gallus gallus domesticus) on gingival wound healing time in Wistar strain rats Florence Meliawati; Nadya Paramitha; Atia Nurul Sidiqa; Saskia Lenggogeni Nasroen
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 2 (2022): Agustus 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i2.36010

Abstract

ABSTRAKPendahuluan: Luka merupakan gangguan kontinuitas lapisan epitel kulit atau mukosa. Gingiva merupakan mukosa mulut yang seringkali mengalami luka yang dapat timbul karena trauma pada perawatan penyakit periodontal ataupun ekstraksi gigi. Membran telur ayam mengandung bahan bioaktif karbohidrat kompleks yang dapat berfungsi mempercepat perbaikan jaringan. Tujuan penelitian menganalisis pengaruh aplikasi gel serbuk membran telur ayam 10% terhadap penyembuhan luka sayat gingiva tikus galur Wistar pada hari ke 3,7, dan 14. Metode: Jenis penelitian eksperimental murni dengan jumlah sampel 30 ekor tikus galur Wistar. Luka sayat sepanjang 3 mm arah horizontal kedalaman 0,25 mm dibuat pada gingiva cekat anterior rahang atas. Kelompok uji terdiri dari 3, yaitu apikasi gel membran telur 10% (n=10), aplikasi povidone iodine 10% sebagai kontrol postitif (n=10), dan aplikasi aquades sebagai kontrol negatif (n=10). Pengamatan panjang luka seluruh sampel dilakukan pada hari ke-0, 3, 7, dan 14. Data yang diperoleh kemudian dianalisis dengan uji Kruskal Wallis dan dilanjutkan uji post hoc Mann Whitney dengan p<0,05. Hasil: Seluruh kelompok mengalami pengurangan panjang luka sayat dimulai dari hari ke 3, 7, hingga 14. Kelompok perlakuan aplikasi gel membran telur 10% dapat menyembuhkan luka sayatan lebih cepat dibandingkan kelompok kontrol positif pada hari ke-3 (1,74±0,14)mm, hari ke-7 (0,81±0,16)mm dan hari ke-14 (0,07±0,14)mm. Pengukuran panjang luka sayat di hari ke-3 dan ke-7 kelompok aplikasi gel membran telur 10% signifikan berbeda dibandingkan kontrol positif (p<0,05). Simpulan: Gel serbuk membran telur 10% dapat mempercepat penyembuhan luka sayat gingiva tikus galur Wistar pada hari ke-3, ke-7 dan ke-14.Kata kunci: luka sayat; membran telur ayam; penyembuhan luka; galur wistar ABSTRACTIntroduction: The epithelial layer of the skin or mucosa is disrupted when there is a wound. The gingiva is the oral mucosa that frequently sustains wounds from trauma that can happen during the treatment of periodontal disease or tooth extraction. Complex carbohydrate bioactive ingredients found in chicken egg membranes, it can speed up tissue repair. This study aimed to analyzed the impact of application egg membrane gel 10% on the length of the wound on Wistar strain on day 3, 7, and 14. Methods: A (3x0.25)mm incision was made on the maxillary anterior attached gingiva Wistar strain rats. There were 3 groups, egg membrane gel 10%, povidone iodine 10% as a positive control, and aquades as a negative control. Observation of wound length for all samples was carried out on days 0, 3, 7, and 14. The data obtained were then analyzed by the Kruskal Wallis test and followed by the Mann Whitney post hoc test with p<0.05. Results: All groups experienced a reduction in the length of the incision starting from days 3, 7, to 14. The egg membrane gel 10% application treatment group healed the incision faster than the positive control group on day 3 (1.74±0.14)mm, day 7 (0.81±0.16)mm, and day 14 (0.07±0.14)mm. The measurement of the length of the incision on days 3 and 7 of the egg membrane gel 10% application group was significantly different from the positive control (p<0.05). Conclusion: egg membrane gel 10% can accelerate the healing of gingival incisions in Wistar strain rats started on days 3 to 14.Keywords: cut wounds; wound healing; chicken egg membrane; wistar strain
CLASSIFICATION OF WINTER IMPACTION OF MANDIBLE THIRD MOLAR ON THE DISTANCE OF THE MANDIBULAR CANALS ON PANORAMIC RADIOGRAPHS AT RSGMP UNJANI Mutiara Sukma Suntana; Saskia Lenggogeni Nasroen; Ilham Fadhilah
Journal of Health and Dental Sciences Vol. 2 No. 3 (2023): Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Impacted teeth are teeth obstructed during the eruption process; the high prevalence of impacted teeth, especially third molars, accompanied by complaints that arise, requires omentectomy. Winter's classification shows various positions of impacted third molars based on angulation. This classification is judged to be describe the relationship of the apical position of the mandibular third molar tooth to the mandibular canal more clearly. The odontectomy procedure requires an excellent preoperative approach to consider the structures around the mandibular third molar, one of which is the mandibular canal, because the canal's proximity to the third molar root can increase the risk of nerve injury during the odontectomy procedure. This study aims to determine the relationship between the third mandibular impaction Winter classification and the mandibular canal distance on panoramic radiographs. The samples of this study were 53 and were grouped according to the position of the impaction vertically, mesioangularly, horizontally, and distoangularly. The study was conducted cross-sectionally by measuring the apical distance of the mandibular third molar to the upper border of the canal on a panoramic radiograph using a manual caliper. The space obtained was statistically analyzed using the eta correlation test. The results showed that 38 and 48 impactions were found to have the most mesioangular impaction, followed by horizontal, vertical, and distoangular paping the least. The mesioangular position measured the distance from the apical third molar of the impacted mandible to the closest distance to the mandibular canal, followed by the horizontal and the longest distance in the vertical position. Statistical tests were conducted to determine the relationship between the mandibular third molars and the mandibular canal. In the test results, the calculated F value on tooth 38 (2.64) and tooth 48 (0.507) was smaller than the F table value (2.794); this indicates there is no significant relationship between the two variables. The absence of a close relationship between the mandibular third molar distance and the mandibular canal indicates the possible low risk of complications in paresthesia after the odontectomy procedure. DOI : 10.54052/jhds.v2n3.p455-466
THE INCIDENCE OF SYSTEMIC DISEASES IN DENTAL PRACTICE AND THE READINESS OF DENTISTS IN THEIR MANAGEMENT Tichvy Tammama; Saskia Lenggogeni Nasroen; Raeny Mutiara Dewi
Journal of Health and Dental Sciences Vol. 2 No. 3 (2023): Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

Systemic disease is a medical condition that can affect dental care and cause complications during and after dental treatment, so it needs special attention and treatment by dentists. Systemic diseases consist of heart disorders and hypertension, diabetes, blood disorders, and others. The purpose of this study is to determine the readiness of dentists to treat patients with systemic diseases. The study was conducted by distributing questionnaires in an electronic form to dentists in West Bandung Regency (KBB) and its surroundings in the form of experience in finding patients with systemic diseases in practice and readiness in their management. Questionnaires were obtained from 83 dentists, with the results describing that 98.8% of dentists had treated patients with systemic diseases. Systemic diseases that were often found in practice are hypertension (96.2%), then diabetes (89.2%), heart disorders (65.1%), asthma (47%), allergies (45.8%), stroke (37.3%), blood disorders (27.7%), kidney disorders (25.3%), thyroid disorders (24.1%), liver disorders (14.5%), and others (2.4%). The results show that dentists in the KBB area were well-prepared to treat patients with systemic diseases. Dental and oral care in patients with systemic diseases were found in daily practice. Based on the research results, most dentists have encountered patients with systemic diseases and are ready to treat patients with controlled systemic diseases. However, when the systemic disease conditions are not governed, the dentist chooses to consult the patient with a general practitioner or internal medicine specialist first. Most dentists in the West Bandung Regency and its surroundings have found patients with systemic diseases who were well-prepared for their management plans at their practice sites. DOI : 10.54052/jhds.v2n3.p489-500
Optimizing examination of methylenetetrahydrofolate reductase gene promoter methylation in cleft lip with or without cleft palate non-syndromic patients using the pyrosequencing method Maskoen, Ani Melani; Rahayu, Nurul Setia; Nasroen, Saskia Lenggogeni
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 3 (2024): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i3.p184-188

Abstract

Background: Cleft lip with or without cleft palate (CL/P) is the most common congenital anomaly found in Indonesia. CL/P is caused by hereditary (genetic) and environmental factors. Environmental factors can result in methylation in the promoter of the methylenetetrahydrofolate reductase (MTHFR) gene, affecting its expression. Methylation takes place at the CpG site found at chromosome 1, coordinates 11,805,406–11,806,509. Pyrosequencing technology can detect the percentage methylation of a gene quickly, simply, and accurately. Purpose: The aim of the study is to optimize detection of methylation of the MTHFR gene using the pyrosequencing method. Methods: Methods used in this study were DNA extraction from blood, DNA bisulfite conversion, polymerase chain reaction (PCR), and methylation detection using CpG pyrosequencing assay. Samples were taken from 20 CL/P patients (C) and 44 normal patients (N). Results: The pyrosequencing method was successful in detecting methylation at three MTHFR gene sites at coordinates 11,805,507–11,805,529. The methylation level at the third site was higher in group C than in group N, while at the first and second positions, group C had a lower methylation level than group N. In general, the percentage of methylation for both groups was low or hypomethylated (less than 5%). Conclusion: The pyrosequencing method can be used to determine methylation levels in the MTHFR gene with the results presented as percentages (quantitative data). Hypomethylation occurs in groups C and N at the coordinates 11,805,507–11,805,529 of the MTHFR gene promoter.
Analysis of FOXE1 rs4460498 and GSTP-1 I105V associated with non syndromic cleft lip and palate among Deutero Malay Subrace in Indonesia Nasroen, Saskia Lenggogeni; Meliawaty, Florence; Tammama, Tichvy; Prasdiaz, Putri Indriani; Choirunnisa, Tias; Maskoen, Ani Melani
Padjadjaran Journal of Dentistry Vol 34, No 3 (2022): November 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no3.39296

Abstract

ABSTRACTIntroduction: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are suspected of having a role in some of the non-syndromic cleft lip and palate (NS CLP) populations worldwide. This study aims to analyze FOXE1 rs4460498 and GSTP-1 I105V polymorphisms associated with NS CLP as the risk factor among Deutero Malay Subrace in Indonesia. Methods: This study was a case-control design, using samples from the venous blood of 102 NS CLP subjects and 102 healthy control subjects. After DNA was extracted, the PCR-RFLPs method was performed using TasI restriction enzyme on 100 blood samples of FOXE1 rs4460498 group and Alw26I restriction enzyme on 105 blood samples of the GSTP-1 I105V group. The Chi-Square test was used with the Kolmogorov Smirnov and Exact Fisher alternatives. Results: T mutant allele (OR= 0.926, p>0.05) and CT genotype (OR= 0.0, p>0.05) of FOXE1 rs4460498 and the G mutant allele (OR= 0.988,p>0.05) and AG genotype (OR= 0.675,p>0.05) of the GSTP-1 I105V are not the risks of NS CLP. Conclusion: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are not associated with non-syndromic cleft lip and palate among Deutero Malay Subrace in the Indonesian population.Key words: deutero malay; FOXE1 rs4460498; GSTP-1 I105V; NS CLP
Polymorphisms of RS7055763 and RS41307258 in TBX22 Gene Haplotype as Risk Factors for Non-syndromic Cleft Palate Indonesian Deutero-Malay Population Susanti, Nita; Ullah, Irfan; Sulaksono, Haura Labibah Salsabil; Nasroen, Saskia Lenggogeni; Maskoen, Ani Melani
Global Medical & Health Communication (GMHC) Vol 12, No 3 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i3.13677

Abstract

Non-syndromic cleft palate (NS-CP) is a multifactorial congenital malformation affected by genetic and environmental factors. The incidence of non-syndromic cleft lip with or without cleft palate (NS-CLP) varies considerably between ethnic groups and geographical regions. TBX22 is a crucial determinant for the formation of intramembranous bone in the posterior hard palate. Therefore, TBX22 is fundamental to palatogenesis and supports normal palate progress. The rs7055763 and rs41307258 polymorphisms in the TBX22 gene are associated with risk factors for NS-CP in the Indonesian Deutero-Malay population. In the previous study, NS-CP still needed to be investigated in the Deutero-Malay population. However, there are different races, mainly for the Deutero-Malay population. This study aims to determine whether rs7055763 and rs41307258 polymorphisms in the TBX22 gene are risk factors for NS-CP in the Deutero-Malay population. This study was conducted in Terpadu Laboratory, Faculty of Dentistry, Universitas Padjadjaran, from February until June 2023. The design of this study was a case-control study. The DNA patient samples were obtained from saliva and whole blood. Moreover, DNA is extracted, and the rs7055763 and rs41307258 segments are analyzed using PCR and Sanger sequencing. PCR data was analyzed by chi-square testing. In this study analysis, polymorphisms of rs7055763 (G>A) and rs41307258 (T>A) in the TBX22 gene show no significant differences between case and control groups, namely 0.911 and 0.645, respectively. However, the genotype in the rs41307258 shows the p-value as 0.027, indicating substantial differences and the OR is 1.390. In conclusion, the rs7055763 and rs41307258 polymorphisms in the TBX22 gene do not appear to be risk factors for developing NS-CP in the Indonesian Deutero-Malay population.
The Role of Transforming Growth Factor-Beta, Fibroblast Growth Factor, Platelet-derived Growth Factor, Epidermal Growth Factor, Insulin-like Growth Factor, Vascular Endothelial Growth Factor, and Sonic Hedgehog in the Non-syndromic cleft lip with or without cleft palate development: A Scoping Review Ullah, Irfan; Khan, Muhammad Salman; Nasroen, Saskia Lenggogeni; Maskoen, Ani Melani; Sarilita, Erli
Chimica et Natura Acta Vol 12, No 3 (2024)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v12.n3.51493

Abstract

Non-syndromic cleft lip with or without cleft palate (NSCLP) birth defect, it imposes an enormous stress on society and requires nutrition, dental, speech, behavioural, and surgical therapies. The NSCLP multifactorial aetiology, including the environment and genetic factors. The environment and genetic factors affect the cellular mechanism, cell proliferation, cell differentiation, and cell migration and signalling pathways. Genetic growth factors including Transforming Growth Factor-Beta (TGF-β), Fibroblast Growth factors (FGFs), Platelet-derived Growth factors (PDGFs), Epidermal Growth factor (EGF), Insulin-like growth factors (IGF), Vascular Endothelial Growth factor (VEGF), Sonic Hedgehog (SHH). The study aims to understand the role of the growth factors “TGF-β, FGFs, PDGFs, EGF, IGF, VEGF, and SHH” in NSCLP development. Preferential Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) standards were followed when performing this scoping review. The 942 articles were extracted, and the following inclusion and exclusive criteria 43 articles were eligible for review. Twenty-seven studies identify 26 genes and 25 single-nucleotide polymorphisms (SNPs)/variants of the growth factors that are a significant risk for NSCLP development. In conclusion, the analysis of diverse populations and growth factors including TGF-β, FGFs, PDGFs, EGF, IGF, VEGF, and SHH were associated with NSCLP. The growth factors were involved in the cellular mechanism, cell proliferation, cell differentiation cell migration and signalling pathways that lead to the pathogenesis of NSCLP.
The effects of IRF6 rs2235373 polymorphism on mRNA expression changes in non-syndromic cleft lip and palate with various phenotypes Nasroen, Saskia Lenggogeni; Maskoen, Ani Melani; Soedjana, Hardisiswo; Soeria Soemantri, Eky Setiawan; Hilmanto, Dany
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.552 KB) | DOI: 10.24198/pjd.vol30no3.17949

Abstract

Introduction: The IRF6 rs2235373 (C/T) is a polymorphism in the intron region that has been associated with non-syndromic cleft lip and palate (NS CLP) among some populations. Polymorphism in introns can also affect the transcription that should be detected through some changes in IRF6 mRNA expression. This study was aimed to evaluate the effects of IRF6 rs2235373 polymorphism on mRNA expression changes in NS CLP with its phenotypes which include complete unilateral (CU) CLP, complete bilateral (CB) CLP, cleft lip only (CLO), and cleft palate only (CPO). Methods: This study was a laboratory analytical study, using samples from 198 individuals in Indonesia (136 individuals with NS CL/P consisted of CU CLP (n=42), CB CLP (n=34), CLO (n=33), CPO (n=27), and 62 individuals as controls). DNA was extracted from venous blood, and the segment of rs2235373 was evaluated by PCR and Sanger sequencing. mRNA expressions analysis preceded by RNA extraction from oral epithelium, then real-time (RT) PCR was performed. mRNA expression changes were analysed by Livak method and Kruskal Wallis test followed by the Mann Whitney test through CC, GT, and TT genotype. Result: The mRNA expression of TT genotype from NS CU CLP and control was 3.36 higher value than CC genotype (p=0.008), and the mRNA expression of CT genotype from NS CB CLP and control was 0.16 lower value than CC genotype (p=0.000). Conclusion: The IRF6 rs2235373 polymorphism of TT genotype tend to cause mRNA overexpression in NS CU CLP, and CT genotype tend to cause mRNA underexpression in NS CB CLP.Keywords: Non-syndromic cleft lip and palate, NS CLP phenotype, IRF6, rs2235373, mRNA expression.
Penyuluhan Kesehatan Gigi Mulut untuk Meningkatkan Pengetahuan Keluarga Pasien Celah Bibir dan Langit-Langit sebagai Implementasi Program Merdeka Belajar Kampus Merdeka Nasroen, Saskia Lenggogeni; Yuslianti, Euis Reni; Meliawaty, Florence; Ardan, Rachman; Isnaeni, Rheni Safira; Irsyad, Widya; Irfianti, Isti
Jurnal Abdimas Kartika Wijayakusuma Vol 3 No 1 (2022): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v3i1.172

Abstract

Masyarakat yang keluarganya mengalami kondisi bibir sumbing atau celah bibir dan langit-langit mayoritas berasal dari kalangan ekonomi lemah. Kelainan bawaan yang bermanifestasi dari bibir atas ke gusi, dan langit-langit yang tidak sepenuhnya menyatu atau berkembang secara terpisah yang dapat disebabkan oleh faktor genetik dan lingkungan. Keluarga yang memiliki riwayat dengan celah bibir dan langit-langit penting mengetahui pengetahuan tentang celah bibir dan langit-langit. Kegiatan pengabdian masyarakat ini dilakukan sebagai implementasi program Merdeka Belajar Kampus Merdeka (MBKM) dosen dan mahasiswa Fakultas Kedokteran Gigi Unjani untuk membantu masyarakat luar kampus yang tidak terjangkau edukasi dari pelayanan kesehatan dengan intensif. Selain itu dilakukan penelitian dengan metode kuesioner kepada para orang tua penderita di Yayasan Pembina Penderita Celah Bibir dan Langit-langit Bandung. Data dianalisis T-test dependent (p<0,05). Hasil menunjukkan terdapat pengaruh signifikan terhadap peningkatan pengetahuan setelah penyuluhan dibandingkan dengan sebelum penyuluhan pada keluarga pasien celah bibir dan langit-langit (p=0,00). Dapat disimpulkan bahwa program MBKM yang dilaksanakan dapat berperan pada perubahan pengetahuan keluarga mengenai kesehatan gigi dan mulut pasien dengan riwayat celah bibir dan langit-langit setelah dilakukannya penyuluhan.