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All Journal JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) JURNAL ILMU DAN TEKNOLOGI PERIKANAN TANGKAP
Ixchel F Mandagi
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Mechanical Engineering

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Study of e-DNA Quality at Fishing Ground of Manado Bay, North Sulawesi Province. Zebua, Nistiarni; Mandagi, Ixchel F.; Masengi, K.W.A; Luasunaung, Alfret; Rumengan, Inneke F. M.; Wulur, Stenly; Makapedua, Daisy M.; Masengi, E. I. K.G.; Sumilat, Deiske A.; Masengi, Akira W. R.; Manoppo, Victoria
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.57404

Abstract

We used the Nansen Bottle Sampler to collect water samples in the deepsea area, ranging from 150 meters to 175 meters in six water points around Manado Bay, to test the quality of e-DNA water samples to detect target species in the fishing area. Therefore, the basis of the case study method with a sampling technique was carried out on July 29 2023 using Power Water Sterivex Kits, water samples were then stored at -250C and were then taken to TBRC, University of the Ryukyus for further laboratory works, such as; eDNA extract, eDNA quality testing, 1st and 2nd PCR and Electrophoresis eDNA analysis processes using MiFish-U primers with a target of 163–185bp and 375 bp, following the MiFish protocol.  Based on the results of the eDNA extract solution, it is known that the quality of eDNA from the 6 sampling sites locations ranged between 2.8 µg/mL – 4.4 µ g/mL, which means a good quality of eDNA. Moreover, it showed that the presence of DNA fragments at Kappa 60◦C Gelelectrophoresis 1st-PCR, 12S rRNA gene product (163–185bp), and Kappa 60◦C and 65◦C Geklelectrophoresis 2nd-PCR Products according to the target amplicon 375 bp. This means we can conduct the next step, the PCR sequence analysis.  Then, eDNA quality testing, 1st and 2nd PCR, and Electrophoresis of e-DNA analysis process were using MiFish-U F/R primers with a target of 375 bp, it is known that the concentration of Nanodrop from the 6 sampling locations ranges between 2.8 µg/mL – 4.4 µg/mL while the core or quality eDNA ranged from 1.56 µg/mL – 2.50 µg/mL. Based on identification results, five types of species were detected; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus and Homo sapiens were generated using eDNA metabarcoding on the mitochondria genome database MitoFish. Keywords: e-DNA, eDNA quality, species target, fishing area, Manado Bay   Abstrak Kami menggunakan Nansen Bottle Sampler untuk mengambil sampel air pada laut dalam berkisar 150meter sampai 175 meter di enam titik perairan Sekitar Teluk Manado, untuk menguji Kualitas e-DNA sample air yang digunakan untuk mendeteksi target spesies pada daerah penangkapan. Selanjutnya dasar metode studi kasus dengan teknik pengambilan sampel secara sampling dilakukan pada tanggal 29 Juli 2023 menggunakan Power Water Sterivex Kits, sapel air disimpan pada -250C yang selanjutnya dibawa ke TBRC, University of the Ryukyus untuk pnelitian laboratorium lanjutan seperti ekstrak eDNA, Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U dengan target 375 bp, mengikuti MiFish protokol. Berdasarkan hasil pengujian larutan ekstrak eDNA diketahui bahwa kualitas eDNA dari 6 titik lokasi sampling berkisar antara 2.8 ng/mL – 4.4 ng/mL dan menunjukkan adanya fragment DNA pada Kappa 60◦C Geklelectrophoresis 1st-PCR Produk 12S rRNA gene (163–185bp, dan Kappa 60◦C dan 65◦C Geklelectrophoresis 2nd-PCR Produk sesuai amplikon target 375 bp. Hal ini berarti dapat dilanjutkan pada tahap analisis sekuens PCR. Pengujian kualitas eDNA, 1st and 2nd PCR dan Elektrophoresis proses analisis eDNA menggunakan primer MiFish-U F/R dengan target 375 bp, diketahui bahwa konsentrasi Nanodrop dari 6 titik lokasi sampling berkisar antara 2.8 µg/mL – 4.4 µg/mL sedangkan kemurnian atau kualitas eDNA berkisar antara 1.56 µg/mL – 2.50 µg/mL. Hasil identifikasi menyatakan lima jenis spesies terdeteksi; Myctophum lychnobium, Selar crumenophthalmus, Photonectes sp., Oreochromis sp. Thunnus obesus, Homo sapiens dihasilkan dengan menggunakan eDNA metabarcoding pada MitoFish database genom mitokondria. Kata kunci : e- DNA, kualitas eDNA, target spesies, daerah penangkapan, Teluk Manado.
Adaptation Strategies of Traditional Handline Fishermen to Coastal Reclamation in Sario District Makaluas, Marselino M.; Manoppo, Victoria; Mamangkey, Noldy G. F.; Luasunaung, Alfret; Mandagi, Ixchel F.; Malalantang, Sjenny S.; Sumilat, Deiske A.
Jurnal Ilmiah PLATAX Vol. 13 No. 2 (2025): ISSUE JULY-DECEMBER 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i2.65929

Abstract

Coastal reclamation in Sario District since 1995 has affected the socioeconomic conditions of coastal communities, particularly handline fishermen. This study aims to identify the socioeconomic conditions of fishermen, analyze internal and external factors using PESTEL and SWOT analyses, and determine appropriate adaptation strategies. The research was conducted from August to November 2025 in three villages within Sario District using a descriptive qualitative and quantitative approach. Results show that fishermen are mostly of productive age with low education levels. Declining income due to distant fishing grounds has driven diversification into side jobs. The PESTEL analysis revealed impacts of reclamation, rising costs, and weak marketing, while SWOT placed fishermen in an aggressive strategy position with strong internal capacity but serious external threats. Adaptive strategies involve pursuing side jobs while preserving traditional fishing livelihoods. Keywords: adaptation strategy; traditional fishermen; coastal reclamation; handline fishing; PESTEL analysis; SWOT analysis Abstrak Reklamasi pantai di Kecamatan Sario sejak 1995 berdampak pada kondisi sosial ekonomi masyarakat pesisir, terutama nelayan pancing ulur. Penelitian ini bertujuan mengidentifikasi kondisi sosial ekonomi nelayan, menganalisis faktor internal dan eksternal menggunakan PESTEL dan SWOT, serta menentukan strategi adaptasi yang tepat. Penelitian dilakukan selama Agustus–November 2025 di tiga kelurahan di Kecamatan Sario dengan pendekatan deskriptif kualitatif dan kuantitatif. Hasil menunjukkan nelayan didominasi usia produktif dan berpendidikan rendah. Penurunan pendapatan akibat jauhnya area tangkap mendorong diversifikasi pekerjaan. Analisis PESTEL mengungkap dampak reklamasi, biaya tinggi, dan lemahnya pemasaran, sementara SWOT menempatkan nelayan pada strategi agresif dengan kekuatan internal baik namun menghadapi ancaman eksternal. Strategi adaptasi dilakukan melalui pekerjaan sampingan tanpa meninggalkan profesi tradisional. Kata kunci: strategi adaptasi; nelayan tradisional; reklamasi pantai; pancing ulur; analisis PESTEL; analisis SWOT
Co-Authors Akira W.R. Masengi Alfred Luasunaung Alfret Luasunaung Alfret Luasunaung Alfret Luasunaung Asano Ishikawa Daniel F. Mokodongan Deiske Adeliene Sumilat, Deiske Adeliene Dien, Heffry Veibert Effendi P. Sitanggang Elvan Sembiring Ernawati Paputungan Fajri Salote Fanny Silooy Frangky E. Kaparang Frangky Ernes Kaparang Fransisco P. T. Pangalila Fransisco P. T. Pangalila Heffry Veibert Dien Hernita Paputungan Inneke F. M Rumengan Ivor L. Labaro Ivor Labaro Javier Montenegro Johny Budiman Jun Kitano K. W.A. Masengi K.W. Alex Maseng Kawilarang W. A Masengi Kawilarang W.A. Masengi Kawilarang W.A. Masengi Kazunori Yamahira Koji Mochida Lefrand Manoppo Lefrand Manoppo Lusia Manu Makaluas, Marselino M. Makapedua, Daisy M. Malalantang, Sjenny S. Mamangkey, Noldy G.F Masamitsu Iwata Masamitsu Iwata Masengi, Akira W. R. Masengi, E. I. K.G. Masengi, K.W.A Mulis Pamikiran, Revols D. Ch. Patrice N.I. Kalangi Renny K. Hadiaty Sandrina Nellah Worang Shanice Rotama Sitorus Shingo Fujimoto Sridelianti Liulondo Taketoshi Sue Takuma Kaito Tirza H. Samosir Tirza H. Samosir Victoria E. N. Manoppo Vivanda O. J. Modaso Vivanda O.J. Modaso Welhelmina Patty Wilhelmina Patty Wulur, Stenly Zebua, Nistiarni