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Journal : Science Midwifery

Magnesium and insulin leaf combination on glucose, low-density lipoprotein and malondialdehyde levels in wistar rats with diabetes mellitus Astari Nurisani; Mamay Mamay; Ana Hidayati Mukaromah; Purwanto AP
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1226

Abstract

Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia due to impaired insulin secretion, insulin receptor sensitivity and/or both. Hyperglycemia can trigger oxidative stress conditions that can increase the risk of micro and macrovascular complications. Preventive efforts need to be done such as giving supplements and traditional therapies that have low side effects, namely magnesium and insulin leaves. This research is experimental type with post test only control group. The samples in this study were 30 male wistar rats weighing 170-300g which had healthy conditions adapted for 7 days, given standard feed and given ad libitum drinking water. The results showed that the glucose level in the treatment group 3 when compared to the negative control group had a percentage decrease of 26.49%. LDL and MDA levels were lower in the combination treatment group of magnesium and insulin leaves when compared to the negative control group. The ANOVA results showed that there was a significant difference between the groups in the glucose test results (p < 0.05), and there was no significant difference between the groups in the LDL and MDA results (p > 0.05). The conclusion of this study was that the best results when compared to negative controls were the combination of magnesium at a dose of 300 mg and insulin leaves at a dose of 300 mg/KgBB had a decreased percentage of LDL levels by 52.38% and MDA levels of 29.11%, while for glucose levels the combination of magnesium doses 300 mg and insulin leaves 75 mg/KgBW had a percentage reduction of 26.49%
Isolation and identification of escherichia coli in well water located in Garut Regency Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1236

Abstract

Water is one of the most important needs for daily life, especially well water. In the Garut district area well water is used for daily activities ranging from washing, drinking, bathing, ablution to being used for drinking water consumption. This study aims to isolate and identify well water from 10 villages located in the Garut Regency. Sampling was done using purposive sampling method. The test was followed by a presumptive test using Lactose Broth (LB), a reinforcing test using 2% BGLB media and a complementary test consisting of EMB agar media and biochemical tests. The results showed that of the 10 well water samples, 7 met the requirements of proper water quality and 3 did not meet the requirements of clean water with MPN values from three villages, namely Kampung Tanjung kamuning 200/ml, Kampung salaawi 241/100ml and Kampung Cipepe 241/100ml. EMB agar media is characterized by the formation of a metallic green color with black spots colonies and biochemical tests that show the well water has been contaminated with Escherichia coli bacteria.