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Journal : Biopendix: Jurnal Biologi, Pendidikan dan Terapan

LITERATURE REVIEW: APLIKASI PENANDA MOLEKULER UNTUK ANALISIS KEANEKARAGAMAN GENETIK HEWAN Mudaningrat, Ajeng; Umaya, Flora; Syahriza, Farah Ayu Afdhila; Anggraito, Yustinus Ulung; Setiati, Ning
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page11-25

Abstract

Background: Molecular markers (DNA markers) are DNA sequences that are easily detected to characterize or detect certain genetic diversity, one of which is in animals. There are various marker systems used with their respective characteristics. Some of the molecular markers include RFLP, RAPD, ISSR, SSR, and SNP. The purpose of this study was to analyze genetic diversity in animals using molecular marker applications.Methods: The method used is literature review using reputable national and international journals.Results: Based on the results of a literature review, the genetic diversity of various species such as catfish, deer, tilapia, crabs, seahorses, ducks, cattle and buffaloes can be analyzed for genetic diversity using various molecular markers either by using PCR-RFLP, RAPD, ISSR, SSR, or SNPs.Conclusion: The application of molecular markers can be used to analyze animal genetic diversity.
LITERATURE REVIEW: METALLOTHIONEIN SEBAGAI PROTEIN PENGIKAT LOGAM UNTUK BIOREMEDIASI LOGAM BERAT Ma'rifah, Amriyah Ummi; Anggraito, Yustinus Ulung; Setiati, Ning
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 2 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue2page186-193

Abstract

Background: The purpose of bioremediation is to remove or reduce dangerous compounds to improve environmental quality, one of which is heavy metals. Metal-binding proteins can minimize the impact of heavy metal pollution from the environment. This metal binding protein can accumulate metals by microorganisms. One of the known metal-binding proteins is metallothioneins (MTs). This protein can minimize the effects of heavy metals in the environment by creating protein-metal ion complex bonds. This review article provides a comprehensive insight into the role of metallothionein as a metal-binding protein for heavy metal accumulation, and the mechanisms involved in heavy metal bioremediation Methods: The method used is a literature review using reputable national and international journals. Results: Based on the results of a literature review, metallothionein can be found in many organisms such as bacteria, humans, plants, invertebrates, and mammals. Metallothionein can accumulate heavy metals that contain highly conserved cysteine residues. These residues allow MTs to bind, transport, and store various essential (Zn and Cu) and non-essential (Cd and Hg) heavy metals via thiolate bonding. Conclusion: Heavy metals are dangerous for the survival of living things and can cause damage to vital functions. One of the metal-binding proteins is metallothionein, which is involved in the organism's tolerance to heavy metal content and can accumulate it, so that the effects of heavy metals can be minimized.
LITERATUR REVIEW: PEMANFAATAN METABARCODING DNA LINGKUNGAN UNTUK MENGANALISIS KEANEKARAGAMAN HAYATI IKAN Anggraeni, Diah Arum; Anggraito, Yustinus Ulung; Setiati, Ning
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 2 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue2page178-185

Abstract

Background: Environmental DNA (eDNA) metabarcoding is a new method for assessing biodiversity in which samples are taken from the environment via water, sediment or air from which DNA is extracted, and then amplified using common or universal primers in a polymerase chain reaction and sequenced using next-generation DNA. sequencing to produce thousands to millions of sequences. From this data, the presence of species can be determined, and overall biodiversity can be assessed.. Methods: The method used is a literature review using reputable national and international journals Results: Based on the results of the literature review, the use of eDNA metabarcoding can be used to analyze fish biodiversity. eDNA metabarcoding can be analyzed with primary data, databases, and bioinformatic pipelines. Conclusion: eDNA metabarcoding has been widely used to study fish communities in lakes and rivers, as well as coastal fish communities. Despite its considerable ability to detect fish species in large aquatic systems, many methods still need to be understood to improve the quality of primers, databases, and pipelines for analyzing fish biodiversity
TINJAUAN PUSTAKA SISTEMATIS: KARAKTERISASI KEANEKARAGAMAN GENETIK SPESIES JERUK (Citrus sp.) MENGGUNAKAN PENANDA SIMPLE SEQUENCE REPEAT (SSR) Oktaviana, Eryka; Setiati, Ning; Anggraito, Yustinus Ulung
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 2 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue2page202-212

Abstract

Background: The biodiversity of citrus plants is very abundant and many are cultivated, creating various types. However, the high demand makes orange farmers feel overwhelmed. This is what causes some orange cultivars to become rare. Characterization of the genetic diversity of citrus germplasm at the molecular level is an important and crucial step that can assist in the breeding and conservation process. The aim of this literature review is to provide information regarding molecular markers regarding the application of SSR molecular markers to analyze the genetic diversity of citrus. Method: The method used is a systematic literature review with the literature sources used coming from articles taken from national journals and international journals indexed by Scopus. Results: The application of molecular markers using the SSR method is useful for identifying and characterizing the genetic diversity of citrus germplasm so that it can assist in breeding and conservation actions. SSR has many advantages both in terms of its use and the results obtained. Conclusion: One application of molecular markers is to analyze plant genetic diversity. Simple Sequence Repeat (SSR) molecular markers can help to analyze the characteristics and genetic diversity of oranges so that they can assist in plant breeding and conservation purposes.
Co-Authors Abdullah Muamar Adinda Berliana Putri Afifah, Diana Iffatul Afifah, Diana Iffatul Agus Prasetyo, Ahmad Ahmad Agus Prasetyo Aida Fariroh, Aida Amin Retnoningsih Andin Irsadi Andreas Priyono Budi Prasetyo Anggraeni, Diah Arum Ardiansari, Anidya Arumsasi, Fitri Astiti, Ikhsana Nuri Ayuwaningsih, Melina Siska Cendikia Hesti Ariyani Citra Tania, Melika Dewi Mustikaningtyas Elin Rahmawati Endah Peniati Enni Suwarsi Rahayu Fatimah Dini Pamungkas Siwi, Fatimah Dini Pamungkas Febriyanto Febriyanto Fitri Arum Sasi, Fitri Arum Fransiska Harahap Hapsari Hidayah, Farida Hidayah, Farida Hidayat Trimarsanto Ibnul Mubarok Isnahardiyanti, Fadhila Istiawan, Edy Izzah, Noor Kris D, Yermia Dita Krispinus Kedati Pukan Kurniahtunnisa Kurniahtunnisa Lail Isro, Abda Ma'rifah, Amriyah Ummi Mahlail, Febrina Icha Marthani, Queen K. A Martien Herna Susanti Maulibulung Sinaga, Na Dame Melati, Dinda Milatuzzahroh, Laily Mohamad Ikhsan Nurulloh Muamar, Abdullah Mudaningrat, Ajeng Mukh Syaifudin Nana Kariada Trimartuti Nikmah, Nailatun Ning Setiati Noor Aini Habibah Nugrahaningsih Wahyu Harini Nugraini, Anisa Ratna Oktaviana, Eryka Pa’ee, Furzani Peryana, Riska Pit’ay, Marisa Fonny Pramesti Dewi Prihandoko, Arief Priskila, Erin Priyantini Widiyaningrum Purnami, Sri Wahyu Putri, Fiky Auliyah Putri, Fiky Auliyah R Susanti R. Susanti, R. Susanti Ragilia Novitasari, Ragilia Rahayu, Enni S. Rani, Bone Kartika Retno Sri Iswari Rizqiyati, Ni'mah Rizqiyati, Ni'mah Rohmah, Sifa Wahdatun Saiful Ridlo Samsul Nizar Septiana Anggraini Septiana Anggraini, Septiana Sigit Saptono Sinaga, Na Dame Maulibulung Siti Alimah Siti Harnina Bintari Siti Rosidah Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ngabekti Sriyadi Sriyadi, Sriyadi SUTIKNO Suwarsi Rahayu, Enni Syahriza, Farah Ayu Afdhila Talitha Widiatningrum Triana, Dwita Umaya, Flora usumawardani, Rini K Yanti Lusiyanti Yuliyani, Rahma