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Journal : Biosaintifika: Journal of Biology

Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium Rahayu, Enni Suwarsi; Habibah, Noor Aini; Anggraito, Yustinus Ulung; Melati, Dinda; Sinaga, Na Dame Maulibulung; Pa’ee, Furzani
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): Inpress December 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.33383

Abstract

The conservation of  Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth.  The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf.  Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.
Co-Authors Abdullah Muamar Adinda Berliana Putri Afifah, Diana Iffatul Afifah, Diana Iffatul Agus Prasetyo, Ahmad Ahmad Agus Prasetyo Aida Fariroh, Aida Amin Retnoningsih Andin Irsadi Andreas Priyono Budi Prasetyo Anggraeni, Diah Arum Ardiansari, Anidya Arumsasi, Fitri Astiti, Ikhsana Nuri Ayuwaningsih, Melina Siska Cendikia Hesti Ariyani Citra Tania, Melika Dewi Mustikaningtyas Elin Rahmawati Endah Peniati Enni Suwarsi Rahayu Fatimah Dini Pamungkas Siwi, Fatimah Dini Pamungkas Febriyanto Febriyanto Fitri Arum Sasi, Fitri Arum Fransiska Harahap Hapsari Hidayah, Farida Hidayah, Farida Hidayat Trimarsanto Ibnul Mubarok Isnahardiyanti, Fadhila Istiawan, Edy Izzah, Noor Kris D, Yermia Dita Krispinus Kedati Pukan Kurniahtunnisa Kurniahtunnisa Lail Isro, Abda Ma'rifah, Amriyah Ummi Mahlail, Febrina Icha Marthani, Queen K. A Martien Herna Susanti Maulibulung Sinaga, Na Dame Melati, Dinda Milatuzzahroh, Laily Mohamad Ikhsan Nurulloh Muamar, Abdullah Mudaningrat, Ajeng Mukh Syaifudin Nana Kariada Trimartuti Nikmah, Nailatun Ning Setiati Noor Aini Habibah Nugrahaningsih Wahyu Harini Nugraini, Anisa Ratna Oktaviana, Eryka Pa’ee, Furzani Peryana, Riska Pit’ay, Marisa Fonny Pramesti Dewi Prihandoko, Arief Priskila, Erin Priyantini Widiyaningrum Purnami, Sri Wahyu Putri, Fiky Auliyah Putri, Fiky Auliyah R Susanti R. Susanti, R. Susanti Ragilia Novitasari, Ragilia Rahayu, Enni S. Rani, Bone Kartika Retno Sri Iswari Rizqiyati, Ni'mah Rizqiyati, Ni'mah Rohmah, Sifa Wahdatun Saiful Ridlo Samsul Nizar Septiana Anggraini Septiana Anggraini, Septiana Sigit Saptono Sinaga, Na Dame Maulibulung Siti Alimah Siti Harnina Bintari Siti Rosidah Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ngabekti Sriyadi Sriyadi, Sriyadi SUTIKNO Suwarsi Rahayu, Enni Syahriza, Farah Ayu Afdhila Talitha Widiatningrum Triana, Dwita Umaya, Flora usumawardani, Rini K Yanti Lusiyanti Yuliyani, Rahma