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Journal : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

Spermatozoa Viability of Filial Ettawa Goat After Sexing Process Kusumawati, Enik Dwi; Leondro, Henny; Susilawati, Trinil; Isnaini, Nurul
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

The use of albumin derived from egg white is a method that is easy to apply, cheap, effective for sexing semen process of separating the spermatozoa X and Y. The viability of Filial Ettawa goatspermatozoa after the sexing process using different percentages of albumin density egg white withcoconut water diluent and egg yolk diluents was examined. The egg white albumin densitytreatments were 3 gradients of 10%, 30% and 50%. Laboratory experiments were performed sexingspermatozoa using the 3 density gradients of egg white albumin with coconut water diluent and eggyolks diluents with a 20 minute incubation time. Each treatment was replicated 10 times. Data wasstatistically analyzed using analysis of variance model (ANOVA). The addition of coconut waterdiluent and egg yolk diluent provide a highly significant difference (p<0.01) in the spermatozoaseparation of the top and bottom layers, but the average value there is no significant difference. Thespermatozoa viability after sperm sexing found that using density gradient egg whites showed betterresults using egg yolk diluent compared to coconut water diluent. The top layer had a mean percentlive sperm of 78.52 ± 8.65% and the bottom layer had mean percent live sperm of 80.29 ± 8.735%.Further research is needed into the  quality of spermatozoa sexing results using varying gradients ofegg whites.Key Words: Sexing, Egg White Albumin Density, Diluent, Egg Yolk, Goat
Differences in The Quality Of Feed on Blood Glucose Levels, Production and Quality Of Milk in Dairy Cattle Leondro, Henny
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

This study aims to determine the effect of different feed quality on blood glucose levels,production and quality of milk in dairy cattle. The material used in this study are 9 Friesian Holsteindairy cows with a weight range of 350-400 kg , lactation levels  I-III , divided  into 3 treatment groupsfeed  are: T0 = 70% forage of corn + concentrate 30% (14% CP); T1 = Forage of Corn 60% + 40%concentrate ( 17% CP); T2 = Forage of Corn 50% + 50% concentrate (20% CP). This research methodused a Randomized Block Design. Data were analyzed by analysis of variance. The averageconsumption of dry matter T0, T1 and T2 respectively 11,95, 11,08 and 10,29 kg. The average bloodglucose concentration at T0, T1 and T2 respectively 53,00, 49,33 and 57,67 mg/dl. The averageproduction of milk at T0, T1 and T2 respectively was 13,60, 16,67 and 17,37 kg. The average fatlevels and fat content of milk on the T0, T1 and T2 respectively 4,8; 4,67; 3,68%  and 0,65, 0,63 and0,64 kg. Average protein levels and protein content of milk on the T0, T1 and T2 respectively was3,56; 3,48; 3,45% and 0,48; 0,57 and 0,59kg. Average lactose levels and lactose content of milk onT0, T1 and T2 respectively was 4,85; 4,69; 4,58% and  0,60, 0,84 and 0,78kg.The conclusion from this study is the feeding with different quality (crude protein content of 14%,17% and 20%) did not significantly affect blood glucose levels, milk production and milk quality indairy cowsKey Words: Feed, Blood Glucose, Production Milk, Quality of Milk
The Quality of Fresh Semen of Bulls at 5 0 C and 24 0 C With or Without Diluent Kusumawati, Enike Dwi; Leondro, Henny
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

The aim of this study was to determine the quality of fresh sperm cattle at 5C and roomtemperature and used of diluent. The study was conducted at the Laboratory Animal HusbandryFaculty of Kanjuruhan University. Research material used was fresh sperm cattle that obtained fromthe Institute for Artificial Insemination (BIB) Singosari Malang. Decrease in motility and viabilitywithout extender were higher (P <0.01) compared with extender. Motility of spermatozoa decreasedto 10% at 12 hours without diluent. While the decrease in motility with diluent was 10% at 24 hours.It is also demonstrated motility of 0% at 96 hours without diluent while the semen with extenderhad 12.3 ± 2.16%. Also on the viability, the reduction reached 20% in diluent usage at 9 hours andwithout diluent at  3 hours. Decrease in motility and viability without diluent were higher (P <0.01) compared with diluent. Motility of spermatozoa decreased to 10% at 6 hours without diluent. Whilemotility with diluent decreased 6% at 6 hours. It is also demonstrated motility 1% at 42 hourswithout the diluent while the semen with diluent had 14.5 ± 0.53%. Also on the viability, on thereduction reached 20% in the use of diluent at 15 and 57 hours without diluent, 0% viability whilethe diluent at 57 hours was 31.171±0.37%. Abnormality in six hours without a diluent has shown adecrease of 20% whereas the diluent is still down 15%. The conclusion of this study is the quality ofspermatozoa in the storage temperature 5oC higher than storage at room temperature. The qualityof spermatozoa at 5oC temperature and room temperature with diluent is higher than withoutdiluent.Key Words: Sperm Quality, Time Storage, Temperature, Fresh Sperm, Diluent o