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All Journal BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Pasir Laut Management of Aquatic Resources Journal (Maquares) AL KAUNIYAH Kreano, Jurnal Matematika Kreatif-Inovatif Jurnal Kelautan Tropis Journal of Biology Education JFMR (Journal of Fisheries and Marine Research) Acta Aquatica: Aquatic Sciences Journal Jurnal Laut Khatulistiwa Biosaintifika: Journal of Biology & Biology Education Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Makara Journal of Technology
Diah Ayuningrum, Diah
Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Other

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ISOLASI DAN PENAPISAN AKTINOMISETES YANG BERPOTENSI MENGHASILKAN ENZIM PROTEASE DARI TAMBAK IKAN BANDENG (Chanos chanos) DI KECAMATAN TUGU, SEMARANG Amanina, Farha Tsabita; Muskananfola, Max Rudolf; Ayuningrum, Diah
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 18, No 4 (2022): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.18.4.188-194

Abstract

Limbah pada sedimen tambak bandeng berupa bahan organik, bersumber dari sisa pakan dan hasil metabolisme. Limbah ini mengandung protein yang tinggi, apabila tidak diproses dengan baik dapat mencemari lingkungan perairan. Aktinomisetes merupakan organisme tanah yang memiliki peran penting dalam proses dekomposisi bahan organik dengan bantuan enzim. Penelitian ini bertujuan untuk mengisolasi aktinomisetes yang mampu menghasilkan enzim protease. Jenis penelitian yang dilakukan yaitu deskriptif eksploratif. Sampel sedimen diambil dari dasar tambak ikan bandeng Kecamatan Tugu, Semarang pada 2 titik outlet tambak dengan metode coring modifikasi. Proses isolasi dilakukan menggunakan media IM5, IM6, IM7 dan IM8 dengan metode pour plate, sedangkan purifikasi dengan media IM6 dan IM8 menggunakan metode streak plate. Isolat diskrining dengan media spesifik Skim Milk Agar untuk melihat zona hambat di sekitar koloni sehingga diketahui Indeks Proteolitik (IP). Sebanyak 11 dari 17 isolat aktinomisetes mampu memproduksi enzim protease, tiga isolat berasal dari media IM6 dan 8 isolat dari media IM8. Diperoleh tiga isolat dengan kemampuan paling baik dalam menghasilkan enzim protease dengan nilai IP diatas 1,5, yaitu isolat VTB 1.1, VTB 1.2 dan YTB 1.2. Penelitian lanjutan perlu dilakukan untuk membuktikan bahwa ketiga isolat tersebut mampu menjadi agen pendekomposisi kandungan protein berlebih pada sedimen tambak.
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol 18, No 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Identifikasi dan Analisis Filogenetik Gastropoda pada Ekosistem Mangrove Tapak Kota Semarang dengan Pendekatan DNA Barcoding Friliana Rindyaneputri, Adella; Ayuningrum, Diah; Febrianto, Sigit; Rudolf Muskananfola, Max
Buletin Oseanografi Marina Vol 14, No 2 (2025): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v14i2.63541

Abstract

Ekosistem mangrove memiliki berbagai peranan ekologis, salah satunya adalah sebagai habitat organisme gastropoda. Gastropoda ekosistem mangrove berperan sebagai bioindikator dan dekomposer. Identifikasi spesies gastropoda secara molekuler di kawasan mangrove penting dilakukan untuk mengetahui spesies-spesies gastropoda yang ditemukan secara akurat. Penelitian ini bertujuan untuk mengidentifikasi spesies gastropoda dan tingkat kekerabatannya di Kawasan Mangrove Desa Tapak Kecamatan Tugu Kota Semarang yang dilaksanakan pada bulan September - November 2023. Metode yang digunakan dalam penelitian ini yaitu deskriptif-kuantitatif. Pengambilan sampel penelitian dilakukan dengan metode purposive sampling. Penanda genetik yang digunakan dalam penelitian ini adalah gen Cytochrome Oxydase I (COI) dengan penyejajaran sekuens DNA menggunakan metode MUSCLE. Analisis filogenetik berbasis algoritma neighbor joining (NJ) 1000 bootstrap pada software MEGA X. Hasil identifikasi molekuler diperoleh bahwa sampel GT1 memiliki kemiripan sebesar 96,12% (Percent Identity) dengan Pirenella alata dan sampel GT2 sebesar 99,69% (Percent Identity) dengan Telescopium telescopium. Hasil analisis filogenetik menunjukkan kladogram kedua spesies ini terdapat pada klade yang berbeda. Klade I terdiri dari sampel GT1 beserta 4 spesies pembanding lainnya dalam 1 genus, yaitu Pirenella alata, Cerithidea microptera, Pirenella nanhaiensis, dan Pirenella incisa. Klade II terdiri dari sampel GT2 beserta 4 spesies pembanding lainnya yaitu Telescopium telescopium, Varicinassa variciferus, Laevilitorina caliginosa, dan Monoplex krebsii.  Mangrove ecosystem has many ecological roles, one is as gastropods habitat. Mangrove ecosystems gastropods act as bioindicators and decomposers. Gastropods mangroves areas molecular identification is important to accurately the types of gastropod species. This research aims to identify gastropods species and the relationship levels in the Mangrove Area of Tapak Village, Tugu District, Semarang conducted from September − November 2023. This research using descriptive-quantitative method. Research sampling using purposive sampling method. The genetic marker used in this research is the Cytochrome Oxydase I (COI) gene with DNA sequence alignment MUSCLE method. Phylogenetic analysis based on the neighbor joining (NJ) 1000 bootstrap algorithm in MEGA X. The results of molecular identification showed that GT1 sample had a similarity of 96.12% (Percent Identity) to Pirenella alata and GT2 sample was 99.69% (Percent Identity) similar to Telescopium telescopium. The results of the phylogenetic of two species analysis show that the cladograms are in different clades. Clade I consist of sample GT1 along with 4 other comparison species in 1 genus, namely Pirenella alata, Cerithidea microptera, Pirenella nanhaiensis, and Pirenella incisa. Clade II consists of sample GT2 along with 4 other comparison species, namely Telescopium telescopium, Varicinassa variciferus, Laevilitorina caliginosa, and Monoplex krebsii.
Analisis Stok Kepiting di Perairan Pekalongan"“Kendal, Jawa Tengah, Indonesia Taufani, Wiwiet Teguh; Saputra, Suradi Wijaya; Ayuningrum, Diah; Sabdaningsih, Aninditia
Jurnal Laut Khatulistiwa Vol 8, No 2 (2025): July
Publisher : Dept. Marine Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/lkuntan.v8i2.95004

Abstract

Kepiting merupakan komoditas perikanan bernilai ekonomi tinggi dan menjadi produk ekspor dari perairan utara Jawa Tengah. Berdasarkan kajian Komisi Nasional Pengkajian Stok Ikan (2022), potensi lestari kepiting di Laut Jawa diperkirakan 7.360 ton per tahun dengan tangkapan diizinkan 5.152 ton per tahun, dan tingkat eksploitasi telah mencapai 0,9. Penelitian ini bertujuan menganalisis pola pertumbuhan, ukuran tangkap pertama (〖CW〗_c), ukuran matang gonad pertama (〖CW〗_m), dan tingkat eksploitasi kepiting bakau (Scylla sp.) di perairan Pekalongan hingga Kendal sebagai upaya mendukung pengelolaan berkelanjutan. Sebanyak 5.545 individu dianalisis, terdiri dari 2.818 jantan dan 2.727 betina. Hasil menunjukkan pola pertumbuhan alometrik negatif (b
SKRINING BAKTERI PENGHASIL ENZIM AMILASE DARI SEDIMEN TAMBAK UDANG VANNAMEI (Litopenaeus vannamei) Novitasari, Diva Triza; Purnomo, Pujiono Wahyu; Jati, Oktavianto Eko; Ayuningrum, Diah; Sabdaningsih, Aninditia
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 2 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.02.15

Abstract

Tambak budidaya udang banyak memproduksi limbah organik yang berasal dari sisa metabolisme dan sisa pakan dari kultivan budidaya. Keberadaan limbah organik yang melimpah dan tidak didampingi dengan manajemen kualitas air, akan mengakibatkan dampak buruk pada kultivan seperti kematian. Proses bioremediasi termasuk salah satu cara untuk membersihkan bahan pencemar pada lingkungan tambak yang dibantu oleh agen biologis yaitu bakteri. Bakteri amilolitik dapat digunakan sebagai agen bioremediator pada lingkungan karena menghasilkan enzim amilase. Amilase merupakan enzim yang mampu menghidrolisis pati menjadi senyawa yang lebih sederhana. Penelitian ini bertujuan untuk mengisolasi bakteri dari sedimen tambak udang vanname dan melakukan skrining enzim amilase menggunakan media Starch Casein Nitrat (SCN). Sedimen diambil pada 5 lokasi tambak pasca panen di Kabupaten Rembang. Sampel diisolasi menggunakan teknik pour plate dan purifikasi dengan teknik streak plate, sedangkan skrining enzim dilakukan dengan teknik dotting. Hasil penelitian yang didapatkan menunjukkan bahwa terdapat 20 isolat bakteri yang diisolasi dari sedimen tambak udang vanname dan 9 isolat bakteri menunjukkan aktivitas enzim amilase, diketahui dari zona bening yang dihasilkan pada isolat setelah ditambahkan iodine.
Pemanfaatan DNA Barcoding untuk Identifikasi Spesies Bivalvia di Ekosistem Mangrove Desa Tapak, Kota Semarang Yustina, Nurul; Rudolf Muskananfola, Max; Ayuningrum, Diah; Febrianto, Sigit
Buletin Oseanografi Marina Vol 14, No 3 (2025): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v14i3.63696

Abstract

Ekosistem mangrove berada pada wilayah intertidal yang memiliki interaksi dinamis antara perairan laut, payau, sungai dan terestrial. Fungsi ekologis ekosistem mangrove sebagai pelindung pantai dari erosi, feeding ground, nursery ground, dan spawning ground bagi biota perairan. Biota yang beragam termasuk moluska kelas bivalvia banyak ditemui di daerah mangrove. Identifikasi spesies bivalvia secara molekuler di kawasan mangrove penting dilakukan untuk mengetahui spesies-spesies bivalvia yang ditemukan secara akurat. Tujuan penelitian ini adalah mengetahui spesies bivalvia di lokasi penelitian dan mengetahui hubungan filogenetik spesies yang telah ditemukan melalui analisis pohon filogenetik. Metode yang digunakan dalam penelitian ini adalah deskriptif kuantitatif dengan metode pengambilan sampel purposive sampling. Penelitian ini berlangsung pada bulan September – Desember 2023. Penanda genetik yang digunakan dalam penelitian ini adalah gen Cytochrome Oxydase I (COI) dengan penyejajaran sekuens DNA menggunakan metode maximum likelihood. Analisis filogenetik berbasis algoritma neighbor joining (NJ) 1000 bootstrap pada software MEGA XI. Hasil data sequencing kemudian dianalisis menggunakan BLAST dan menunjukkan sampel BM1 memiliki kemiripan sebanyak 99% dengan spesies Donax incarnatus sedangkan 99,85% pasa sampel BM2 dengan dengan spesies Perna viridis. Hasil analisis filogenetik menunjukkan kladogram kedua spesies ini terdapat pada klade yang berbeda. Klade I terdiri dari sampel BM1 beserta 4 spesies pembanding lainnya yaitu Donax incarnatus, Donax obesulus, Donax deltoides, dan Donax faba. Klade II terdiri dari sampel BM2 beserta 4 spesies pembanding lainnya yaitu Perna viridis, Perna canaliculus, Perna perna, dan Perna Picta.
Identification and Phylogenetic Analysis of Blue Swimming Crab (Portunus sp.) in The Northern Waters of Jepara Used DNA Barcoding Lazuardhi, Risqi; Sabdaningsih, Aninditia; Ayuningrum, Diah; Saputra, Suradi Wijaya; Hartoko, Agus; Haeruddin, Haeruddin
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.38705

Abstract

The Awur Bay and Kartini Beach in Jepara, Central Java, are fishing areas with blue swimming crab as a main catch. This study aims to examine the species of blue swimming crabs captured in the Northern Waters of Jepara from different locations, analyze the molecular characteristics based on gender differences, obtain GenBank accession numbers as genetic conservation, and construct their phylogenetic tree. The research used a quantitative descriptive method. Samples were collected using purposive sampling from fishermen's catches and selected based on the difference in crab claws and carapace color, with a total of 4 samples. The samples were extracted using a DNA extraction kit and tested using Polymerase Chain Reaction (PCR) to visualize mitochondrial DNA (COI gene) and processed using MEGA 11. BLAST results showed that the similarity of the samples to Portunus pelagicus ranged from 97.02% to 99.42%. The different colors of claws and carapace did not affect the DNA sequence composition, hence indicating sexual dimorphisms. The DNA sequences from this study were deposited to GenBank with accession number LC836055.1-LC836058.1. Based on the phylogenetic tree construction, the clade of all samples shows a close relationship with the species Portunus pelagicus.
Tourism suitability and community perception on development of the Cengklik Reservoir tourism, Boyolali Regency Firdaus, Nur Salsabila; Purwanti, Frida; Ayuningrum, Diah; Sulardiono, Bambang
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 10: No. 3 (December, 2023)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v10i3.12598

Abstract

Cengklik Reservoir is one of the water and waterfront tourism destinations in the Boyolali Regency which has an area of 327,44 ha. The condition of Cengklik Reservoir is currently undergoing revitalization and tourism development. The purpose of this study was to analyse the suitability of reservoir waters as a tourist attraction based on the Tourism Suitability Index, to determine the perceptions of tourists potential in the reservoir, and community perceptions of tourism development in the reservoir. The research was conducted in March - April 2023. The research methods used a case study with quantitative analysis to calculate the value of the Tourism Suitability Index and qualitative analysis to determine the perception of tourism potential from 30 tourist respondents and tourism development from 30 community respondents. Results of the TSI calculation of the Cengklik Reservoir for boat tours amounted to 2.40 (suitable) and fishing amounted to 3.00 (very suitable). The tourists perception of the Cengklik Reservoir as well as the communitys perception of tourism development were rated positively. These results indicate that the Cengklik Reservoir is suitable for tourism which is supported by the communitys perception of the tourist developmentKeywords: Cengklik Reservoir; Tourism Development; Tourism Potential; Tourism Suitability Index.
Study of the catch of white shrimp (Penaeus merguiensis) using Wangkong fishing gear in Demak Waters Nurlaela, Yulfa Rahmanissa; Ayuningrum, Diah; Prakoso, Kukuh
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 12: No. 2 (August, 2025)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v12i2.17910

Abstract

White shrimp (P. merguiensis) is the dominant catch caught in Demak waters and has high economic value. The fishing gear used by fishermen is wangkong fishing gear. This research aims to determine the composition and size structure of the catch, as well as the relationship between length and weight and sex ratio. The research was carried out in April and May 2024. The research method used was a survey method. The shrimp sampling method used is the census method. The target catch of fishermen from Menco Village, namely Penaeus merguiensis, was 87.10% and fishermen from Babalan Village, namely Penaeus merguiensis, amounted to 66.63%. The non-target catches were Mugil spp, Plotosuscanius, Rastrelliger spp, Scylla serrata, Oratosquilla oratoria, and Anguilla bicolor. The length class mode for white shrimp in Menco Village is 74.4 – 79.2 mm and Babalan Village is 96.6 – 106.5 mm. The relationship between length and weight of male white shrimp from Menco Village was obtained as W= 0.000353293L2.724 and female white shrimp from Menco Village was obtained as W= 0.000244543L2.837. The relationship between length and weight of male white shrimp from Babalan Village was obtained as W= 0.001095644L2.402 and female white shrimp from Babalan Village was obtained as W= 0.000167463L2.960. The sex ratio for white shrimp showed that the ratio between males and females in Menco Village was 1:1.10 and Babalan Village was 1:1.36. The growth pattern of P. merguiensis is negative allometric and more female shrimp are caught than male shrimp. Keywords: Catch; Demak Waters; Wangkong; WhiteShrimp (P. merguiensis)
Hydrodynamic Characteristics and Sediment Distribution Patterns in Wulan Delta Estuary, Demak, Indonesia Muskananfola, Max Rudolf; Febrianto, Sigit; Ayuningrum, Diah
Makara Journal of Technology Vol. 27, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Wulan Delta, located in Wedung, Demak, was formed due to the sedimentation process of the Wulan and Serang rivers. Sediment transport starts from rivers to the sea; the process carries nutrients and various chemicals derived from agricultural, industrial, or household activities. This study aims to analyze the characteristics of hydrodynamics, suspended sediment, and bottom sediments in the Wulan Delta waters, Demak. Field data were collected in transitional season two (October) 2022. The obtained data were then analyzed in the laboratory at the Faculty of Fisheries and Marine Science. Results showed that the speed of the surface current in the study area ranged from 0 to 0.1 m/s in spring tide conditions with a surface current pattern coming from the direction of Semarang toward Jepara and 0–0.18 m/s in neap tide conditions with a current pattern coming from the direction of Jepara toward Semarang. The Wulan Delta has a sedimentary characteristic dominated by fine silt, which is as much as 82%–98%, with a deposition characteristic dominated by wave energy that causes successful sediment sorting with a sorting index of −2.25 to 2 and strongly asymmetrical to a small size with a skewness curve value of two.
Co-Authors Agus Hartoko Agus Sabdono Amanina, Farha Tsabita Aninditia Sabdaningsih Bahry, Muhammad Syaifudien Bambang Sulardiono Bangun, M. Fadhillah Rasyid Beginer Subhan Clara, Jessika Oktaviani David Nugroho Fadhila, Zalfa Langgeng Fahlevi, Arief Reza Febrianto, Sigit Fenetiruma, Syamsudin Firdaus, Nur Salsabila Frida Purwanti Friliana Rindyaneputri, Adella Haeruddin Haeruddin Haryani, Nisrina Septi Herettrenggi, Rosa Junaedy, Ahmad Kintani, Novia Indah Kukuh Prakoso, Kukuh Kusumarani, Dheni Lazuardhi, Risqi Lundin, Kennet Malaihollo, Jantje Jacobus Max Rudolf Muskananfola Muttaqin, Ahmad Sabilul Niniek Widyorini Norma Afiati Novitasari, Diva Triza Nur Handayani Nur Hidayah Nurhuda, Izza Siti Nurlaela, Yulfa Rahmanissa Oktavianto Eko Jati Prehadi, Prehadi Pujiono Wahyu Purnomo Rahayu, Sari Sistyawati Rakhim, Lutfi Nur Ratna Diyah Palupi Raunsai, Jhoni Elias Raweyai, Oktofianus Obaja Rizaldi, Rahmat Rosari, Arinda Rudolf Muskananfola, Max Santoso Budi Widiarto Sianturi, Yenti Agustina Sombo, Hendrik Sri Mulyani Endang Susilowati, Sri Mulyani Endang Suradi Wijaya Saputra Suryanti Taufani, Wiwiet Teguh Utari, Erni Dwi Yustina, Nurul Zebua, Vanda Florist