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Efektivitas nanoemulsi gel daun sirih hijau (Piper betle l.) terhadap penurunan ketebalan biofilm bakteri Staphylococcus aureus ATCC 6538: eksperimental laboratoris Maliky, Zaid; Abdul Kodir, Ade Ismail; Agusmawanti, Prima; Pratiwi, Rosa
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.57721

Pendahuluan: Penyakit periodontal adalah infeksi pada rongga mulut yang diakibatkan oleh bakteri dengan persentase kasus 74,1%. Salah satu bakteri yang berperan adalah Staphylococcus aureus. Bakteri ini merupakan salah satu bakteri gram positif yang berperan pada kolonisasi awal. Sirih hijau merupakan tanaman herbal yang memiliki kandungan senyawa antibakteri seperti flavonoid, alkaloid, tanin dan saponin. Teknologi nanoemulsi gel memiliki keunggulan untuk mempermudah penghantaran obat karena ukuran partikelnya yang sangat kecil. Penelitian ini bertujuan untuk menganalisis efektivitas nanoemulsi gel daun sirih hijau terhadap penurunan ketebalan biofilm bakteri Staphylococcus aureus. Metode: Metode penelitian ini menggunakan eksperimental laboratorium in vitro yang berjumlah 25 sampel dibagi menjadi 5 kelompok yang terdiri dari nanoemulsi gel daun sirih hijau konsentrasi 30%, 40%, 50%, gel metronidazole dan aquadest. Pembacaan hasil uji ketebalan biofilm diukur dengan Optical Density menggunakan ELISA-reader. Hasil: Hasil rerata nilai optical density pada kelompok formulasi nanoemulsi gel daun sirih hijau, konsentrasi 50% memiliki nilai terendah (2,732) dibandingkan 30% (3,478) dan 40% (3,352) yang artinya konsentrasi 50% paling efektif dibandingkan konsentrasi 30% dan 40%. Hasil uji One Way Anova didapatkan hasil p<0,05 menunjukkan adanya pengaruh nanoemulsi gel daun sirih hijau terhadap penurunan ketebalan biofilm bakteri Staphylococcus aureus. Simpulan: Formulasi nanoemulsi gel daun sirih hijau efektif dalam menurunkan ketebalan biofilm bakteri Staphylococcus aureus.The effectiveness of green betel leaf (piper betle l.) nanoemulsion gel on reducing of staphylococcus aureus ATCC 6538 biofilm thickness: laboratory experimentalIntroduction: Periodontal disease is an infection in the oral cavity caused by bacteria, with a case prevalence of 74.1%. One of the bacteria involved is Staphylococcus aureus. This bacterium is a Gram-positive organism that plays a role in early colonization. Green betel (Piper betle) is a medicinal plant that contains antibacterial compounds such as flavonoids, alkaloids, tannins, and saponins. Nanoemulsion gel technology has the advantage of enhancing drug delivery due to its very small particle size. This study aims to analyze the effectiveness of green betel leaf nanoemulsion gel in reducing the biofilm thickness of Staphylococcus aureus bacteria. Methods. This research used an in vitro laboratory experimental method involving 25 samples divided into 5 groups, consisting of green betel leaf nanoemulsion gel at concentrations of 30%, 40%, 50%, metronidazole gel, and aquadest. The thickness of the biofilm was measured using an ELISA reader by reading the optical density. Results. The average optical density values in the green betel leaf nanoemulsion gel formulation group showed that the 50% concentration had the lowest value (2.732) compared to 30% (3.478) and 40% (3.352), indicating that the 50% concentration was the most effective among the three tested concentrations. The One-Way ANOVA test results showed p < 0.05, indicating that the green betel leaf nanoemulsion gel had a significant effect on reducing the biofilm thickness of Staphylococcus aureus bacteria. Conclusion. It can be concluded that the green betel leaf nanoemulsion gel formulation is effective in reducing the biofilm thickness of Staphylococcus aureus bacteria.

Comprehensive Analysis of Oral Health-Related Quality of Life (OHRQoL) in Periodontitis Patients: Evaluating the Impact of Non-Surgical and Surgical Periodontal Therapy Ratnawati, Irma Dewi; Rizky, Novi Sagita; Styaningrum, Yunita; Rahman, Erwid Fatchur; Aditya, Grahita; Putri, Nabella Devyanna; Pratiwi, Rosa
Jurnal Medali Vol 7, No 2 (2025): Media Dental Intelektual August 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.7.2.84-90

Introduction: Periodontitis is a chronic inflammatory condition that affects the supporting structures of the teeth. Due to its often asymptomatic progression in early stages, timely diagnosis is frequently challenging.Objective: This study aimed to assess the impact of non-surgical and surgical periodontal treatments on the Oral Health-Related Quality of Life (OHRQoL) among individuals diagnosed with periodontitis.Methods: An observational analytic study with a cross-sectional design was conducted using a pre-test and post-test group approach. The study involved 100 patients aged 18–60 years who met predefined inclusion and exclusion criteria and underwent periodontal therapy either non-surgical or surgical at Sultan Agung Islamic Dental and Oral Hospital (RSIGM) between August and December 2024. The OHIP-14sp questionnaire was used to measure OHRQoL across seven domains using a Likert scale. Data were analyzed using descriptive statistics and paired t-tests to evaluate differences between pre- and post-treatment scores.Results: A marked reduction in OHIP-14sp scores was observed across all domains following treatment. The most pronounced improvements occurred in the domains of social disability (−46.2%) and functional limitation (−45.1%). Overall, the total OHIP-14sp score improved by 43.9%. All domains exhibited statistically significant differences between baseline and follow-up scores (p < 0.001). Conclusion: The findings indicated that both non-surgical and surgical periodontal therapies contributed significantly to enhancing patients’ quality of life by alleviating physical discomfort and improving psychological and social well-being.

EFEKTIVITAS PENINGKATAN PENGETAHUAN TENTANG BAHAYA MEROKOK PADA GIGI DAN MULUT MELALUI MEDIA BUKU SAKU Pratiwi, Rosa; Dewi Ratnawati, Irma; Ismail Abdul Kodir, Ade
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 8, No 5 (2025): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v8i5.1887-1893

Merokok telah menjadi gaya hidup masyarakat, baik pada orang dewasa maupun pada orang tua. Rokok merupakan salah satu ancaman terbesar bagi kesehatan masyarakat. Rongga mulut merupakan jalan atau tempat kontak pertama dari asap hasil pembakaran rokok. Hal ini menyebabkan rongga mulut mudah terpapar karena menyerap hasil pembakaran rokok. Gigi dan jaringan lunak seperti gusi dapat mengalami kerusakan akibat paparan asap rokok. Tujuan dari pengabdian masyarakat ini adalah untuk meningkatkan pengetahuan dan kesadaran akan bahaya merokok terutama terhadap gigi dan rongga mulut. Metode yang digunakan adalah penyuluhan tentang bahaya merokok melalui media buku saku. Hasilnya setelah 2 minggu dilakukan evaluasi melalui kuesioner lebih dari setengah jumlah responden (60%) memiliki tingkat pengetahuan baik. Hal ini menunjukkan bahwa media buku saku cukup efektif untuk meningkatkan pengetahuan warga Kelurahan Kaligawe. Buku saku dinilai dapat membantu warga memvisualisasikan materi yang diberikan. Selain itu, juga bersifat praktis dan bisa disimpan sehingga anggota keluarga atau kerabat lain yang tidak hadir dalam penyuluhan bisa ikut membaca.

Antibacterial Effectivity of Nanoemulgel Phaleria macrocarpa’s leaf on the Porphyromonas gingivalis ATCC 33277 Biofilm Thickness Pratiwi, Rosa; Ratnawati, Irma Dewi; Ismail, Ade; Nurhapsari, Arlina; Amelia, Rosalita
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.168-174

Porphyromonas gingivalis is an oppoturnistic microorganism in periodontal disease that is formed at the stage maturation of biofilm formation. One of the natural materials biofilm reduction is the Phaleria macrocarpa. Phaleria macrocarpa’s leaf contains chemical compounds such as flavonoids, tannins, alkaloids, saponins that function as antibacterials.The aim of this research is to determine the effectiveness of the nanoemulsion of the gel Phaleria macrocarpa’s leaf on the thickness of the P. gingivalis bacterial biofilm. The research design was carried out with a post test only with a total of 28 samples, then divided into 4 groups of treatment of Phaleria macrocarpa’s leaf extract nanoemulsion gel preparations with variations in concentrations of 30%, 40%, and 50%, as well as a control group of 25% metronidazole gel. The reading of the anti-biofilm thickness test results was measured by OD (Optical density) using an ELISA-reader. One way Anova parametric test among obtained p<0.000 results showing the effect of Phaleria macrocarpa’ leaf gel nanoemulsion extract on the decrease in the thickness of the Porphyromonas gingivalis bacterial biofilm. It can be concluded that the nanoemulsion gel formulation of Phaleria macrocarpa’s leaf extract concentrations of 30%, 40%, and 50% is effective, with a concentration 50% was most effective against reducing the thickness of the bacterial biofilm Porphyromonas gingivalis.

Antibacterial Effectivity of Nanoemulgel Phaleria macrocarpa’s leaf on the Porphyromonas gingivalis ATCC 33277 Biofilm Thickness Pratiwi, Rosa; Ratnawati, Irma Dewi; Ismail, Ade; Nurhapsari, Arlina; Amelia, Rosalita
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.168-174

Porphyromonas gingivalis is an oppoturnistic microorganism in periodontal disease that is formed at the stage maturation of biofilm formation. One of the natural materials biofilm reduction is the Phaleria macrocarpa. Phaleria macrocarpa’s leaf contains chemical compounds such as flavonoids, tannins, alkaloids, saponins that function as antibacterials.The aim of this research is to determine the effectiveness of the nanoemulsion of the gel Phaleria macrocarpa’s leaf on the thickness of the P. gingivalis bacterial biofilm. The research design was carried out with a post test only with a total of 28 samples, then divided into 4 groups of treatment of Phaleria macrocarpa’s leaf extract nanoemulsion gel preparations with variations in concentrations of 30%, 40%, and 50%, as well as a control group of 25% metronidazole gel. The reading of the anti-biofilm thickness test results was measured by OD (Optical density) using an ELISA-reader. One way Anova parametric test among obtained p<0.000 results showing the effect of Phaleria macrocarpa’ leaf gel nanoemulsion extract on the decrease in the thickness of the Porphyromonas gingivalis bacterial biofilm. It can be concluded that the nanoemulsion gel formulation of Phaleria macrocarpa’s leaf extract concentrations of 30%, 40%, and 50% is effective, with a concentration 50% was most effective against reducing the thickness of the bacterial biofilm Porphyromonas gingivalis.

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