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All Journal Journal of Food and Pharmaceutical Science Pharmascience Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Window of Health : Jurnal Kesehatan Jurnal Mandala Pharmacon Indonesia Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia Mejuajua Pharmaceutical Reports Journal Microbiology Science Journal of Food and Pharmaceutical Sciences Indonesian Journal of Jamu MPSJ
Selpida Handayani, Selpida
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Journal : Pharmaceutical Reports

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Determination of Flavonoids Content of Gringsingan Stems (Mesosphaerum suaveolens (L) Kuntze) Extracted by Microwave Assisted Extraction Handayani, Selpida; Aminah, Aminah; Badu, Nurul Fadillah
Pharmaceutical Reports Vol 4, No 1 (2025): (March) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i1.357

Abstract

Gringsingan plant (Mesosphaerum suaveolens (L) kuntze) is an plant belonging to the lamiaceae family originating from Sinjai district, South Sulawesi. This plant is empirically used by the people of Sinjai district as a medicine to relieve stomach aches. The modern extraction method used is microwave-assisted extraction, which is an extraction method that uses microwave radiation to speed up extraction by heating the solvent quickly and efficiently. The aim of this research was to determine the value of total flavonoid content in Gringsingan (Mesosphaerum suaveolens (L) kuntze) stems extract based on microwave-assisted extraction. This research was carried out experimentally in the laboratory. The stages of this research began with preparing test samples, testing the quality of flavonoid compounds, and ended with determining the total flavonoid content of gringsingan stems, which then measured their absorption using a UV – Vis spectrophotometer. In this study, the results were obtained that the Gringsingan stem extract was positive for containing flavonoids based on identification results using the Wilstater reagent. This sample was also declared positive for containing flavonoids based on the thin layer chromathography (TLC) test using the AICI3 specific reagent. Determination of the total flavonoid content of Gringsingan stem extract based on microwave assisted extraction. Obtained was 17.949 mgQE/g.
Determination of Tannin Content of Gringsingan Stem Extract (Mesosphaerum suaveolens (L) Kuntze) by the UV-Vis Spectrophotometry Handayani, Selpida; Aminah, Aminah; Nurliadi, Indah
Pharmaceutical Reports Vol 4, No 2 (2025): (October) Pharmaceutical Reports
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/pharmrep.v4i2.378

Abstract

The Gringsingan plant (Mesosphaerum suaveolens (L.) Kuntze) is widely used in traditional medicine and is known for its diverse pharmacological properties. Traditionally, it has been utilized to treat various ailments such as tumors, malaria, headaches, cancer, fever, stomach pain, yellow fever, rheumatism, constipation, urethritis, diarrhea, and kidney disorders, as well as to act as an analgesic, antiseptic, antispasmodic, and depurative agent. The present study aimed to determine the total tannin content in the stem extract of Gringsingan obtained through Microwave-Assisted Extraction (MAE) and to evaluate its potential as a bioactive compound source. Microwave-assisted extraction was selected because of its advantages, including shorter extraction time, reduced solvent consumption, and efficient recovery of active compounds compared to conventional methods. This experimental research was conducted in the laboratory and consisted of several stages: sample preparation, qualitative testing for tannin presence, and quantitative determination of total tannin content. Qualitative identification using 1% FeCl₃ reagent produced a dark blue to greenish-black coloration, confirming the presence of tannins in the Gringsingan stem extract. The result was further supported by thin-layer chromatography (TLC) testing with the same reagent, which yielded consistent positive results. Quantitative analysis of the total tannin content was carried out using UV–Visible spectrophotometry, with absorbance measured at the maximum wavelength for gallic acid. The analysis revealed that the tannin concentration in the Gringsingan stem extract was 7.487 mg GAE/g extract, indicating that this plant possesses measurable levels of tannins that contribute to its traditional medicinal value.
Co-Authors Abdul Rasyid, Faradiba Amaliah Dahlia, Andi Aminah Aminah Amran, Farizah Dhaifina Anasya, Andi Fara Andina, Velda Annisa, Khairun Aulia Wati, Aulia Badu, Nurul Fadillah Fahirah, Jihan Faradiba Hasnaeni Hasnaeni hasnaeni, Hasnaeni Herwin Herwin, Herwin Hossani, Rahni Ida Kurniawati, Ida Imani, Fitril Indah Indah Indah Pratiwi Lalo, Ahmad Majid, Nini Nirmala Mamat Pratama, Mamat Nonci, Faridha Yenni Nurliadi, Indah Nurlina Nurlina Nurlina Putri, Andra Audina Putri, Aulia Dinda Rahmawati, Junita Eka Rais Razak, Rais Rezki Amriati Syarif, Rezki Amriati Risda Waris, Risda Sari, Felisa Ananda Syahrana, Nur Azizah Tahir, Karlina Amir Utami, Fadhillah Resky Virsa Handayani Wenni, Asyam Hafidz Wisdawati Wisdawati, Wisdawati